Curriculum Vitae: Cédric Orelle EDUCATION RESEARCH

Curriculum Vitae: Cédric Orelle
EDUCATION
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2004
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1999
Ph.D., Biochemistry – CNRS/University of Lyon, France
Master’s degree, Biochemistry– University of Lyon, France. Rank: 1/34.
Bachelor’s degree, Biochemistry – University of Lyon, France. Rank: 1/155.
RESEARCH EXPERIENCE
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2015-CURRENT
Scientist of the CNRS (CR1)
Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université Lyon
Laboratory of Dr. Jean-Michel Jault
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2011-15
Research Assistant Professor
University of Illinois (Chicago, IL, USA)
Laboratory of Pr. Alexander S. Mankin
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2005-10
Post-Doctoral Research Associate
Purdue University (West Lafayette, IN, USA), 5 years
Baylor College of Medicine (Houston, TX, USA), 1 year
Laboratory of Dr. Amy L. Davidson
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2000-04
Ph.D. in Biochemistry
- Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/ Université Lyon
Laboratory of Dr. Attilio Di Pietro
- 6 months spent at McGill University (Montréal, Canada).
Laboratory of Pr. Philippe Gros
PUBLICATIONS
1- Geourjon C., Orelle C., Steinfels E., Blanchet C., Deleage G., Di Pietro A. and Jault J.-M. (2001). A
common mechanism for ATP hydrolysis in ABC transporter and helicase super-families. Trends Biochem.
Sci., 26 (9), 539-544.
2- Chami M., Steinfels E., Orelle C, Jault J.-M., Di Pietro A., Rigaud J.-L. and Marco S. (2002). Threedimensional structure by cryo-electron microscopy of YvcC, an homodimeric ATP-binding cassette
transporter from Bacillus Subtilis. J. Mol. Biol., 315 (5), 1075-85.
3- Steinfels E., Orelle C., Dalmas O., Penin F., Miroux B., Di Pietro A. and Jault J.-M. (2002). Highly
efficient over-production in E. coli of YvcC, a multidrug-like ATP-Binding Cassette transporter from
Bacillus subtilis. Biochim. Biophys. Acta, 1565 (1), 1-5.
4- Orelle C., Dalmas O., Gros P., Di Pietro A. and Jault J.-M. (2003). The conserved glutamate residue
adjacent to the Walker-B motif is the catalytic base for ATP hydrolysis in the ATP-Binding Cassette
transporter BmrA. J. Biol. Chem, 278 (47), 47002-8.
5- Steinfels E.*, Orelle C.*, Fantino J.-R., Dalmas O., Rigaud J.-L., Denizot F., Di Pietro A. and Jault J.-M.
(2004). Characterisation of YvcC (BmrA), a multidrug ABC transporter constitutively expressed in Bacillus
subtilis. Biochemistry, 43 (23), 7491-7502. *Equal contribution
6- Dalmas O., Orelle C., Foucher A.-E., Crouzy S., Di Pietro A. and Jault J.-M. (2005) The Q-loop
disengages from the first intracellular loop during the catalytic cycle of the multidrug ABC transporter
BmrA. J. Biol. Chem., 280 (44):36857-64.
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7- Orelle C., Gubellini F., Durand A., Marco S., Lévy D., Gros P., Di Pietro A. and Jault J.-M. (2008).
Conformational change induced by ATP binding in the multidrug ATP-binding cassette transporter BmrA.
Biochemistry, 47 (8): 2404-12.
8- Davidson A.L., Dassa A., Orelle C. and Chen J. (2008). Structure, function and evolution of bacterial
ATP-binding cassette systems. Microbiol. Mol. Biol. Rev., 72 (2): 317-64.
9- Orelle C., Ayvaz T., Everly R.M., Klug C.S. and Davidson A.L. (2008). Both maltose-binding protein and
ATP are required for nucleotide-binding domain closure in the intact maltose ABC transporter. Proc. Natl.
Acad. Sci. USA, 105 (35): 12837-42.
10- Khare D., Oldham M.L., Orelle C., Davidson A.L. and Chen J. (2009). Alternating access in maltose
transporter mediated by rigid-body rotations. Mol. Cell., 33 (4): 528-36.
11- Alvarez F.J.D., Orelle C. and Davidson A.L. (2010). Functional Reconstitution of an ABC Transporter in
Nanodiscs for Use in Electron Paramagnetic Resonance Spectroscopy. J. Am. Chem. Soc., 132 (28): 9513–
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12- Orelle C., Alvarez F.J.D., Oldham M.L., Orelle A., Wiley T., Chen J. and Davidson A (2010). Dynamics
of α-helical subdomain rotation in the intact maltose ATP-binding cassette transporter. Proc. Natl. Acad.
Sci. USA, 107 (47): 20293-98.
13- Orelle C., Szal T., Klepacki D., Shaw K.J., Vázquez-Laslop N. and Mankin A.S. (2013). Identifying the
targets of aminoacyl-tRNA synthetase inhibitors by primer extension inhibition. Nucleic Acids Res., 41
(14):e144.
14- Orelle C., Carlson S., Kaushal B., Almutairi M.M., Liu H., Ochabowicz A., Quan S., Pham V.C., Squires
C.L., Murphy B.T. and Mankin A.S. (2013). Tools for characterizing bacterial protein synthesis inhibitors.
Antimicrob. Agents Chemother., 57 (12): 5994-6004.
15- Orelle C.*, Carlson E.D.*, Szal T., Florin T., Jewett M.C. and Mankin A.S. (2015). Protein synthesis by
ribosomes with tethered subunits. Nature, 524: 119-124. *Equal contribution
→ Highlighted in Nature, Nature Methods and Chemistry & Biology
16- Alvarez F.J.D., Orelle C.*, Huang Y., Bajaj R., Everly R.M., Klug C.S. and Davidson A.L. (2015). Full
Docking of the Maltose-bound Binding Protein Stabilizes a Semi-open ATP-binding Cassette Dimer in the
Maltose Transporter. Mol. Microbiol. 98 (5), 878–894. *Corresponding author
17. Marks J.P., Kannan K., Roncase E.J., Klepacki D., Kefi A., Orelle C., Vázquez-Laslop N., Mankin A.S.
(2016). Context-specific inhibition of translation by ribosomal antibiotics targeting the peptidyl transferase
center. Proc. Natl. Acad. Sci. USA, 113 (43): 12150-55.
→ Highlighted in Nature Reviews Microbiology
BOOK CHAPTERS
Cédric Orelle*, Michael L. Oldham* and Amy L. Davidson (2014). The maltose ABC transporter: where
structure meets function. “Membrane Transport Mechanism: 3D structure and beyond” in the Springer
Series in Biophysics, Vol. 17: 181-205. *Equal contribution
Cédric Orelle and Jean-Michel Jault (2016). Structures and transport mechanisms of the ABC efflux pumps.
“Efflux-Mediated Antimicrobial Resistance in Bacteria: Mechanisms, Regulation and Clinical
Implications” in the Springer series, pages 73-98
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PATENT
Alexander Mankin, Michael Jewett, Cédric Orelle, Erik Carlson, Teresa Szal. “Tethered Ribosome and
Methods of Making and Using thereof”. International Pub. No. WO/2015/184283, published 12 March 2015.
RESEARCH FUNDING
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2016-17: Grant from FINOVI foundation (140,000 €) to Cédric Orelle and Jean-Michel Jault
2015: Grant from Fondation pour la Recherche Médicale (45,000 €) for a Research Assistant (1 year)
2012-13: Grant NIH R03 DA035191 ($78,651 total cost) to Alexander S. Mankin and Cédric Orelle
2004-05: 1-year Post-doctoral fellowship, Fondation pour la Recherche Médicale (27,600 €)
2003-04: 6-month extension Ph.D. fellowship, Fondation Pour la Recherche Médicale (7500 €)
2003: Travel award granted by the FEBS (Federation of European Biochemical Societies) for the 4th
FEBS Advanced Lecture Course, Gosau, Austria, 1-8 March 2003.
2002: Travel award granted by the SFBBM for the “Forum des Jeunes Chercheurs” (Dijon, France)
2001: EURODOC travel fellowship granted by the “Région Rhône-Alpes” for six months in the
laboratory of Pr. Philippe GROS, McGill University, Montréal, Canada (3811 €).
2000 : 3-year PhD fellowship from « Ministère de la Recherche »
OTHERS
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Supervision: 15 students, 4 research assistants, 2 PhD students (50% and 30%)
Oral presentations: 9
Poster presentations: 10
Professional services: reviewer for scientific journals (J. Biol. Chem., Mol. Microbiol., J. Gen. Physiol.,
Res. Microbiol., J. Appl. Microbiol., Lett. Appl. Microbiol.) ; reviewer for grants (IDEX Sorbonne Paris
Cité, 2015 ; Chercheur(se)s d'Avenir Languedoc-Roussillon, 2015; "Equipe mixte laboratoire-entreprise"
de la Région Hauts-de-France, 2016) ; Science Fair Judge, Gunsaulus Elementary Scholastic Academy,
Chicago (2012, 2013); PhD advisory committee, Denis Lacabanne (2015-17)
Awards: 2014 Best Poster ($300), Molecular Biology Research Building Research Day, University of
Illinois at Chicago; 2001 Life Sciences Award (760 €) from University of Lyon
Certifications and trainings: Radioactive safety training (McGill University, 2002; Purdue University,
2006; University of Illinois, 2011); “Grant Writing” course, passed with grade A, Purdue University,
2007; Mammalian cell culture training (Purdue University, 2007); CNRS training “Polar lipids and
liposomes” (2016)
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