New-procedurefor-the-diagnosis-and-prognosis-of

New procedure for the diagnosis and prognosis of endometriosis
Abstract ID : 1549
Soumis par : JULIA VALLVE JUANICO Le 2016-03-04 10:29:08
Nom de la catégorie : SEUD CONGRESS
Typologie : Communication orale / Oral communication
Statut : validé
Autorisation de diffusion : Yes/Oui
------------------------------------Endometriosis is a benign estrogen dependent disease that affects around 15% of the women at reproductive age. It is
defined as extra uterine growth of endometrial tissue and it causes pelvic pain and/or infertility. There are no diagnostic
tools, except from surgery. Therefore, our objective is to develop a non-invasive diagnostic test for the disease.
In a previous study, endometrial cells from GFP mice were introduced into peritoneal cavity of RFP mice. GFP+ cells
were found in the stromal compartment of eutopic endometrium of RFP mice three months after surgery, suggesting that
they migrated from the endometriosis lesion. Arrays and immunofluorescence (IF) showed that GFP+ cells co-expressed
cytokeratin (CK, epithelial marker) and Leucine-rich Repeat Containing G protein-Coupled Receptor 5 (LGR5, stem cell
marker of the intestine). We hypothesize that these cells play a role in the genesis of endometriosis through the
Epithelial Mesenchymal Transition process.
IF analysis on paraffin embedded tissue of mice confirmed that GFP+ cells co-localize with LGR5 and E-cadherin
(ECAD, epithelial marker). LGR5+ cells in epithelium and stroma of human eutopic endometrium from patients and
donors by FACS and IF. LGR5+ cells co-localize aberrantly with ECAD and CK in the stroma from 17 and 22 of 26
endometriotic patients respectively, whereas there is no colocalization in none of 11 donors.
We performed a pilot study using RNA-sequencing of the LGR5+/- cells from uterine biopsies from four patients of each
type of endometriosis and healthy donors to identify a genetic signature of the disease and its subgroups. Our results
show that LGR5+ cells have hematopoietic origin and they are not involved in the development of the disease.
Moreover, we studied the gene expression of the total cell population of the uterine biopsies from the same patients. We
developed a classifier of the most significant differentiated genes between the groups excluding the genes that vary
along the menstrual cycle. It has to be verified with another technique and further validated increasing the sample size.
This project would led us to improve the diagnosis of the endometriosis and maybe to distinguish between the disease
subtypes.
------------------------------------Mots clefs : endometriosis, biomarkers, diagnosis
Auteurs :
Références : , , ,
Auteurs
Vallvé Juanico Júlia 1, Suárez Maria Elena 2, Castellví Josep 3, Gil Moreno Antonio 4, Santamaria Costa Xavier 5,
1. Gynecology, IVI Barcelona, Barcelona, SPAIN
2. Department f Gynecology, Vall d'Hebron University Hospital, Barcelona, SPAIN
3. Department of Pathology, Vall d'Hebron University Hospital, Barcelona, SPAIN
4. Group of Biomedical Research in Gynecology, Vall d'Hebron University Hospital, Barcelona, SPAIN
5. G, IVI Barcelona, Barcelona, SPAIN
Auteurs (raw format)
Júlia Vallvé Juanico - email : [email protected] Etablissement : IVI Barcelona Service : Gynecology Ville : Barcelona
Pays : SPAIN Présentateur : Oui
Maria Elena Suárez - email : [email protected] Etablissement : Vall d'Hebron University Hospital Service :
Department f Gynecology Ville : Barcelona Pays : SPAIN Présentateur : Non
Josep Castellví - email : [email protected] Etablissement : Vall d'Hebron University Hospital Service :
Department of Pathology Ville : Barcelona Pays : SPAIN Présentateur : Non
Antonio Gil Moreno - email : [email protected] Etablissement : Vall d'Hebron University Hospital Service : Group
of Biomedical Research in Gynecology Ville : Barcelona Pays : SPAIN Présentateur : Non
Xavier Santamaria Costa - email : [email protected] Etablissement : IVI Barcelona Service : G Ville : Barcelona
Pays : SPAIN Présentateur : Non