biographical sketch

BIOGRAPHICAL SKETCH
NAME
POSITION TITLE
Dufrane Denis
Chief Scientific Officer/Co-founder Novadip
Biosciences
Professor – Université catholique de Louvain (UCL)
EDUCATION/TRAINING
INSTITUTION AND LOCATION
Catholic University of Louvain
Catholic University of Louvain
DEGREE
M.D.
M.Sc.
YEAR(s)
2000
2001
Catholic University of Louvain
Ph.D.
2006
FIELD OF STUDY
Medicine
Cellular and Molecular
Biology
Cell Transplantation/
Encapsulation
A. Positions and Honours.
2000-2002:
Research and Development assistant - University Tissue Bank (Prof. C Delloye,
University Clinical Hospital St Luc, Catholic University of Louvain).
2003-2004:
Research Assistant at the Department of Surgery, Catholic University of Louvain.
Research in the “Experimental surgery Laboratory” (Prof. P. Gianlello).
2004-2007:
Teaching Assistant at the Department of Physiology, Catholic University of Louvain.
Research in the “Endocrinology and Metabolism Unit” (Prof. JC. Henquin) and in the
“Experimental surgery Laboratory”.
2007-2009:
Medical director of Beta cell Laboratory – University Clinical Hospital Saint-Luc;
Catholic University of Louvain .Research and Development position – Bone Tissue
bank; University Clinical Hospital St-Luc, Catholic University of Louvain.
2008-2009:
Operational Manager - Bone Tissue bank; University Clinical Hospital St-Luc, Catholic
University of Louvain.
2008-2015:
Principal Investigator of the UCL-HIA-002 clinical study - University Clinical Hospital St
Luc, Catholic University of Louvain.
2009-2015:
Consultant and Islets Transplant Program Responsible, Abdominal Transplantation Unit
- University Clinical Hospital St Luc, Catholic University of Louvain.
2009-2015:
Medical director of the Endocrine Cell Therapy Unit (Islets and Adipose Stem cells
Bank) – University Clinical Hospital Saint-Luc; Catholic University of Louvain.
2009-2015:
Head of Bone Tissue Bank; University Clinical Hospital St-Luc, Catholic University of
Louvain.
2011-2015:
Head of Tissue/Cells Therapy Center, University Clinical Hospital St-Luc, Catholic
University of Louvain.
2014-2015:
Head of Skin Bank; University Clinical Hospital St-Luc, Catholic University of Louvain.
2013-present: Co-founder and Chief Scientific Officer of Novadip Bioscience
2014-present: Member of Belgium Superior Health Council
2014-present: Professor – Université catholique de Louvain (UCL)
B. Awards:
2000:
2005:
2007:
2007:
Prix de la” BELGIUM ANIMAL CELL TECHNOLOGY SOCIETY »
Prix:”Frezenius Poster Prize” de l’ “EUROPEAN SOCIETY OF TRANSPLANTATION”
Young investigator Award de la “INTERNATIONAL PANCREAS AND ISLET
TRANSPLANT ASSOCIATION”.
Prix Claude Simon – Faculté de Médecine UCL
2007:
2009:
2011:
PhD thesis Award de la “Belgium Transplant Society”
Prix Vandevelde de la Faculté de Médecine-UCL
Prix spécial du jury de la Fédération Wallonie-Bruxelles : « Fonds de Biotechnologie »
- FBBF Award 2011 – Research on Advanced Cell Therapy.
C. Field of expertise:
Islets transplantation
Islets encapsulation
Tissue engineering
Adipose and bone marrow mesenchymal stem cells
Biomaterials: biocompatibility, cytotoxicity
Good Manufacturing Practice
Good Clinical Practice
Cell and Tissue banking (Muskulo-skeletal tissues, Skin tissues, Stem cells)
Advanced cell therapy
Xenotransplantation
Pre-clinical diabetic models
D. Scientific society membership:
Biomaterial Swiss Society; International Pancreatic and Islets Transplantation Society; Belgian Transplantation
Society; European Society of Transplantation; Tissue Engineering and Regenerative Medicine International
Society; Membre du Collège des Alumni de l’Académie Royale Belge des Sciences,Des lettres et des Beauxarts; European Pancreas and Islets Transplantation Association; Cell Transplantation Society; International
Xenotransplantation Association.
E. Scientific responsibility:
- Membre of editorial board of journals: Transplantation » (01/2012-…), Scientifica (2012-….), World Journal
of Gastroenterology (2013-…).
- Reviewer of international journals: Biomaterials; Methods and Findings in Experimental and Clinical
Pharmacology; Pancreas; American Journal of Transplantation; Transplantation; Journal Of Materials
Science: Materials In Medicine; Xenotransplantation; Journals of Biomaterials Applications; Tissue
engineering; New England Journal of Medicine; Nature Protocols; Cytotherapy; Diabetes; Annals of
surgery; Regenerative Medicine; Cell & Tissue Research.
- Reviewer for international fund: Programme de recherche d’initiative régionale (Bretagne –
France, 2006) ; Diabetes UK Foundation (2008,2014) ; Foundation for Polish Science (2010);
Diabetes (2013).
- Reviewer of international congress: International Congress of the Transplantation Society
(Sydney, 2008); International Pancreas and Islets Transplantation Association (Geneva, 2005;
Minneapolis, 2007; Prague, 2011, Monterey 2013, IPITA/IXS/CTS Melbourne 2015), European
Association of Tissue banking (Brussels, 2013), European surgery for surgical research
(Budapest, 2013).
F. Scientific activity:
Publications – Communications- Patents
N total of citations
H-Index global (Google Scholar)
112
1240
20
(I) 43 published manuscripts on Pubmed
Human Adipose-Derived Mesenchymal Stem Cells in Cell Therapy: Safety and Feasibility in Different
"Hospital Exemption" Clinical Applications.
Vériter S, André W, Aouassar N, Poirel HA, Lafosse A, Docquier PL, Dufrane D. PLoS One. 2015 Oct
20;10(10)
Dynamics of glucose-induced insulin secretion in normal human islets. Henquin JC, Dufrane D, KerrConte J, Nenquin M. Am J Physiol Endocrinol Metab. 2015 Epub ahead of print]
Autologous adipose stromal cells seeded on a human collagen matrix for dermal regeneration in
chronic wounds: clinical proof of concept. Lafosse A, Desmet C, Aouassar N, Andre W, Hanet MS,
Beauloye C, Vanwijck R, Poirel HA, Gallez B, Dufrane D. Plast Reconstr Surg. 2015 Apr 24. [Epub
ahead of print]
Local complications of massive bone allografts: an appraisal of their prevalence in 128 patients.
Delloye C, van Cauter M, Dufrane D, Francq BG, Docquier PL, Cornu O. Acta Orthop Belg. 2014
Jun;80(2):196-204.
Bioengineered sites for islet cell transplantation.Vériter S, Gianello P, Dufrane D. Curr Diab Rep. 2013
Oct;13(5):745-55.
Critical size bone defect reconstruction by an autologous 3D osteogenic-like tissue derived from
differentiated adipose MSCs. Schubert T, Lafont S, Beaurin G, Grisay G, Behets C, Gianello P, Dufrane
D. Biomaterials. 2013 Jun;34(18):4428-38.
Improvement of subcutaneous bioartificial pancreas vascularization and function by coencapsulation of
pig islets and mesenchymal stem cells in primates. Vériter S, Gianello P, Igarashi Y, Beaurin G,
Ghyselinck A, Aouassar N, Jordan B, Gallez B, Dufrane D. Cell Transplant. 2014;23(11):1349-64.
Galactosyl-knock-out engineered pig as a xenogenic donor source of adipose MSCs for bone
regeneration. Schubert T, Poilvache H, Galli C, Gianello P, Dufrane D. Biomaterials. 2013
Apr;34(13):3279-89.
Macro- or microencapsulation of pig islets to cure type 1 diabetes. Dufrane D, Gianello P. World J
Gastroenterol. 2012 Dec 21;18(47):6885-93.
Improvement of pig islet function by in vivo pancreatic tissue remodeling: a "human-like" pig islet
structure with streptozotocin treatment. Vériter S, Aouassar N, Beaurin G, Goebbels RM, Gianello P,
Dufrane D. Cell Transplant. 2014;22(11):2161-73.
Beware of the commercialization of human cells and tissues: situation in the European Union. Pirnay
JP, Vanderkelen A, Ectors N, Delloye C, Dufrane D, Baudoux E, Van Brussel M, Casaer MP, De Vos D,
Draye JP, Rose T, Jennes S, Neirinckx P, Laire G, Zizi M, Verbeken G. Cell Tissue Bank. 2012
Aug;13(3):487-98.
Pig islet for xenotransplantation in human: structural and physiological compatibility for human clinical
application. Dufrane D, Gianello P. Transplant Rev (Orlando). 2012 Jul;26(3):183-8.
The enhanced performance of bone allografts using osteogenic-differentiated adipose-derived
mesenchymal stem cells. Schubert T, Xhema D, Vériter S, Schubert M, Behets C, Delloye C, Gianello P,
Dufrane D. Biomaterials. 2011 Dec;32(34):8880-91.
The impact of hyperglycemia and the presence of encapsulated islets on oxygenation within a
bioartificial pancreas in the presence of mesenchymal stem cells in a diabetic Wistar rat model. Vériter
S, Aouassar N, Adnet PY, Paridaens MS, Stuckman C, Jordan B, Karroum O, Gallez B, Gianello P,
Dufrane D. Biomaterials. 2011 Sep;32(26):5945-56.
Beta-5 score to evaluate pig islet graft function in a primate pre-clinical model. Igarashi Y, D'hoore W,
Goebbels RM, Gianello P, Dufrane D. Xenotransplantation. 2010 Nov-Dec;17(6):449-59.
Alginate macroencapsulation of pig islets allows correction of streptozotocin-induced diabetes in
primates up to 6 months without immunosuppression. Dufrane D, Goebbels RM, Gianello P.
Transplantation. 2010 Nov 27;90(10):1054-62.
A genomic-based approach identifies FXYD domain containing ion transport regulator 2
(FXYD2)gammaa as a pancreatic beta cell-specific biomarker. Flamez D, Roland I, Berton A, Kutlu B,
Dufrane D, Beckers MC, De Waele E, Rooman I, Bouwens L, Clark A, Lonneux M, Jamar JF, Goldman
S, Maréchal D, Goodman N, Gianello P, Van Huffel C, Salmon I, Eizirik DL. Diabetologia. 2010
Jul;53(7):1372-83.
Formalin fixation could interfere with the clinical assessment of the tumor-free margin in tumor
surgery: magnetic resonance imaging-based study. Docquier PL, Paul L, Cartiaux O, Lecouvet F,
Dufrane D, Delloye C, Galant C. Oncology. 2010;78(2):115-24.
In vivo selection of biocompatible alginates for islet encapsulation and subcutaneous transplantation.
Vériter S, Mergen J, Goebbels RM, Aouassar N, Grégoire C, Jordan B, Levêque P, Gallez B, Gianello P,
Dufrane D. Tissue Eng Part A. 2010 May;16(5):1503-13.
Pig islets for clinical islet xenotransplantation. Dufrane D, Gianello P. Curr Opin Nephrol Hypertens.
2009 Nov;18(6):495-500.
Native pancreatic alpha-cell adaptation in streptozotocin-induced diabetic primates: importance for pig
islet xenotransplantation. Dufrane D, Maillart JF, Aouassar N, Goebbels RM, Guiot Y, Gianello P.
Xenotransplantation. 2009 May-Jun;16(3):152-63.
Human tissue allograft processing: impact on in vitro and in vivo biocompatibility. Fawzi-Grancher S,
Goebbels RM, Bigare E, Cornu O, Gianello P, Delloye C, Dufrane D. J Mater Sci Mater Med. 2009
Aug;20(8):1709-20.
Dissecting the instant blood-mediated inflammatory reaction in islet xenotransplantation. Goto M,
Tjernberg J, Dufrane D, Elgue G, Brandhorst D, Ekdahl KN, Brandhorst H, Wennberg L, Kurokawa Y,
Satomi S, Lambris JD, Gianello P, Korsgren O, Nilsson B. Xenotransplantation. 2008 JulAug;15(4):225-34.
Pig islet xenotransplantation into non-human primate model. Dufrane D, Gianello P. Transplantation.
2008 Sep 27;86(6):753-60.
[Correction of a diabetes mellitus type 1 on primate with encapsulated islet of pig pancreatic
transplant]. Gianello P, Dufrane D. Bull Mem Acad R Med Belg. 2007;162(10-12):439-49; discussion
449-50. French.
Regeneration of abdominal wall musculofascial defects by a human acellular collagen matrix. Dufrane
D, Mourad M, van Steenberghe M, Goebbels RM, Gianello P. Biomaterials. 2008 May;29(14):2237-48.
Non-heart-beating donor, 10-year experience in a Belgian transplant center. Malaise J, Van Deynse D,
Dumont V, Lecomte C, Mourad M, Dufrane D, Squifflet JP, Van Ophem D, Verschuren F, Meert P, Thys
F, El Gariani A, Wittebole X, Laterre PF, Hantson P. Transplant Proc. 2007 Oct;39(8):2578-9.
Nutrient control of insulin secretion in perifused adult pig islets. Dufrane D, Nenquin M, Henquin JC.
Diabetes Metab. 2007 Dec;33(6):430-8.
Nutrient control of insulin secretion in isolated normal human islets. Henquin JC, Dufrane D, Nenquin
M. Diabetes. 2006 Dec;55(12):3470-7.
[Treatment diabetes by transplantation of insulin secreting cells. Part II--prevention of rejection of the
transplanted cells]. Pokrywczyńska M, Drewa T, Włodarczyk Z, Dufrane D. Pol Merkur Lekarski. 2006
May;20(119):619-21. Review. Polish.
[Treatment diabetes by transplantation of insulin secreting cells. Part I]. Pokrywczyńska M, Drewa T,
Włodarczyk Z, Dufrane D. Pol Merkur Lekarski. 2006 May;20(119):615-8. Review. Polish.
In vitro evaluation of acute cytotoxicity of human chemically treated allografts. Dufrane D, Cornu O,
Verraes T, Schecroun N, Banse X, Schneider YJ, Delloye C. Eur Cell Mater. 2001 Jan 10;1:52-8;
discussion 58.
Parameters favouring successful adult pig islet isolations for xenotransplantation in pig-to-primate
models. Dufrane D, D'hoore W, Goebbels RM, Saliez A, Guiot Y, Gianello P. Xenotransplantation. 2006
May;13(3):204-14.
Six-month survival of microencapsulated pig islets and alginate biocompatibility in primates: proof of
concept. Dufrane D, Goebbels RM, Saliez A, Guiot Y, Gianello P. Transplantation. 2006 May
15;81(9):1345-53.
The influence of implantation site on the biocompatibility and survival of alginate encapsulated pig
islets in rats. Dufrane D, Steenberghe Mv, Goebbels RM, Saliez A, Guiot Y, Gianello P. Biomaterials.
2006 Jun;27(17):3201-8.
Low molecular weight dextran sulfate: a strong candidate drug to block IBMIR in clinical islet
transplantation. Johansson H, Goto M, Dufrane D, Siegbahn A, Elgue G, Gianello P, Korsgren O,
Nilsson B. Am J Transplant. 2006 Feb;6(2):305-12.
Streptozotocin-induced diabetes in large animals (pigs/primates): role of GLUT2 transporter and betacell plasticity. Dufrane D, van Steenberghe M, Guiot Y, Goebbels RM, Saliez A, Gianello P.
Transplantation. 2006 Jan 15;81(1):36-45.
Non-heart-beating donor, renewal of an old procedure: a Belgian experience. Van Deynse D, Dumont
V, Lecomte C, Squifflet JP, Mourad M, Verschuren F, Dufrane D, Hantson P, Malaise J. Transplant
Proc. 2005 Jul-Aug;37(6):2821-2.
Is the expression of Gal-alpha1,3Gal on porcine pancreatic islets modified by isolation procedure?
Dufrane D, Goebbels RM, Guiot Y, Gianello P. Transplant Proc. 2005 Jan-Feb;37(1):455-7.
A simple method using a polymethylpenten chamber for isolation of human pancreatic islets. Dufrane
D, Goebbels RM, Guiot Y, Squifflet JP, Henquin JC, Gianello P. Pancreas. 2005 Apr;30(3):e51-9.
Impact of porcine islet size on cellular structure and engraftment after transplantation: adult versus
young pigs. Dufrane D, Goebbels RM, Fdilat I, Guiot Y, Gianello P. Pancreas. 2005 Mar;30(2):138-47.
Indirect cytotoxicity evaluation of pseudowollastonite. Dufrane D, Delloye C, McKay IJ, De Aza PN, De
Aza S, Schneider YJ, Anseau M. J Mater Sci Mater Med. 2003 Jan;14(1):33-8.
Clinical application of a physically and chemically processed human substitute for dura mater. Dufrane
D, Marchal C, Cornu O, Raftopoulos C, Delloye C. J Neurosurg. 2003 Jun;98(6):1198-202.
Physical and chemical processing for a human dura mater substitute. Dufrane D, Cornu O, Delloye C,
Schneider YJ. Biomaterials. 2002 Jul;23(14):2979-88.
(II) 124 Presentations=
79 : Oral Presentations
45 : Poster Presentations
77: First author
01: Co-author
49: Last author
(III) 37 lectures on invitation
- “Islets Transplantation for the treatment of Type 1 Diabetes”. Dufrane D. Chronic Care Center
Foundation; Beyrouth (Liban), Juin, 2004.
- “Correction d’un diabète de type 1 chez le primate par une greffe d’îlots pancréatiques porcins
encapsulés ». Gianello P-Dufrane D, Académie Royale de Médecine ; Bruxelles, Octobre, 2007.
- “Encapsulation where we are?”. Dufrane D. 25 years of Pancreas Transplantation on Belgium;
Liège, Novembre 2007.
- “Islets encapsulation to cure Type 1 diabetes”. Dufrane D and Gianello P, Roche Diagnostics,
Symposium « Diabetes : From Research to Medical Practice »; La Hulpe, Avril, 2008.
- « Dons de tissus ». Dufrane D, B. Pelleriaux, C. Delloye. “Don d’organes et Transplantation au
quotidien. Cliniques universitaires de Mont-Godinne, Avril 2008.
- “Diabetes correction by pig islets monolayer device in non-human primates”. Gianello P and
Dufrane D. Tissue Engineering and Biomaterials technologies. Juvenile Diabetes Research
Foundation ; San Diego (USA), Décembre, 2008.
- “Islets cell xenotransplantation: update on recent progress and future perspectives”. Dufrane D
and Gianello P. International symposium xenotransplantation – DFG- Transregio research group
xenotransplantation together with the 12th minisymposium xenotransplantation of the German
working group xenotransplantation. Berlin, June, 2009.
- “L’encapsulation d’îlots xenogéniques”. Dufrane D et Gianello P. 15th NAT – Beta cell replacement
in diabetes. Nantes, Juin, 2009.
- “Encapsulated pig islets transplants in non-human primates”. Dufrane D. TTS new key opinion
leader (nKOL) meeting 2009. Jackson Hole. June, 2009.
- « Pig islets encapsulation : Pre-clinical to clinical studies ». Dufrane D et Gianello P. 2009
Transplantation center and transplantation research institute joint symposium. Seoul, Corée du
Sud, 12 Septembre 2009.
- “Pig islets xenotransplantation”. Dufrane D et Gianello P. American Transplant Congress 2010.
San Diego, USA, April 2010.
- “Bone substitute and osteoinduction » Dufrane D. 8ème Journée dentaire de l’UCL. Bruxelles,
Belgique, 28-29 Mai 2010.
- “Pig islets xenotransplantation”. Dufrane D et Gianello P. American Transplant Congress 2010.
San Diego, USA, April 2010.
- “Pig islets xenotransplantation”. Gianello P et Dufrane D. 16th NAT – Xenotransplantation.
Nantes, Juin, 2010.
- “Encapsulated islets transplantation”. Dufrane D. Polish Transplantation Society. Varsovie 22th23th October 2010.
- “Transplantation d’îlots”. Dufrane D. Groupement Belge des Pédiatres de Langue Francaise. 19
November 2010. Charleroi.
- “Don de tissus”. Dufrane D. Actualités infirmières en soins intensifs. Bruxelles, Belgique. 27
January 2011.
- “La transplantation d’îlots dans le traitement du diabète”. Journée scientifique de l’ASMA.
Bruxelles, 23 February 2011.
- “La transplantation d’îlots encapsulés dans le traitement du diabète ». 8ème congrès UCL
d’Endocrino-Diabétologie Société francophone d endocrinologie-diabétologie. Bruxelles, 5 March
2011.
- «Islets xeno- and allotransplantation with alginate sheet”. Dufrane D. Juvenile Diabetes Research
Foundation encapsulation workshop, New York, USA, 22-23 March 2011.
- « Le point sur la biologie osseuse. De la Cellule au substitut osseux ». Dufrane D. 3rd
International congress of minimally invasive foot and ankle surgery. Brugge, 26-28 May 2011.
- « Islet Microencapsulation ». Dufrane D. 13th World Congress of International Pancreas and
Islets Transplantation Society. Prague, Czech Republic, 01-04 June 2011.
- « La transplantation d’îlots de Langerhans dans le traitement du diabète de type 1 ». Dufrane D.
Société Francophone du Diabète Paramédicale, Paris 25 November 2011.
- « Adipose mesenchymal stem cells for bone reconstruction ». Dufrane D. Stem cells and
regenerative Medicicine – International conference. Uppsala 28-29th November 2011.
- “Encapsulated pancreatic islets”. Dufrane D. et Gianello P. Stem cells and regenerative
Medicicine – International conference. Uppsala 28-29th November 2011.
- « Le futur de la greffe d’îlots ». Dufrane D. Alternatives biologiques et innovations dans le diabète
de type 1. Lyon 11 Mai 2012.
- «Étude du potentiel clinique d’une greffe d’îlots de Langerhans encapsulés ». Dufrane D. Séance
de l’Académie Royale de Médecine. Bruxelles 16 Juin 2012.
- « Challenges pf porcine islets isolation». Dufrane D. Pre-Congress Symposium 2: Islet Isolation IPITA Monterey September 24-27th september 2013.
- «Encapsulation of xenogenic islets ». Dufrane D. IPITA Monterey USA 24-27th september 2013.
- « Adipose stem cells for bone defect reconstruction. Dufrane D. EATB, Brussels 20-22 th
November 2013
- « Encapsulation xenogenic islets ». Dufrane D. ADPIT. Igls Austria 26-28th January 2014.
- « Utilisation des allogreffes ostéo-tendineuses », Dufrane Journées 2014 - 20èmes journées de
formation – Association francaise des coordinateurs hospitaliers, Touquet, France, 4-6 June 2014.
- « 3D printing tissue engineering », Dufrane D, QED conference – 3D printing revolution, Brussels
10 June 2014.
- « Bone tissue reconstruction: from tissue to cell transplantation ». 9th Yound European Scientist
meeting, Porto, 18-21th september 2014.
- “Potential of Autologous 3D Adipose Stem Cells Graft for Bone Reconstruction”. Translational
Regenerative Medicine. Leipzig, 21-22th October 2014.
- « 3D printing tissue engineering in medicine», Dufrane D, QED conference – 3D printing
revolution, Brussels 04th November 2014.
- “Encapsulation designs for cell transplantation : macroencapsulation, microencapsulation and
conformal coating », Dufrane D, Tbilisi Cell Therapy Symposium 9-10 October, Tbilisi, Georgia.