Upgrading the UltiMate 3000 RSLCnano System with ProFlow

Upgrading the UltiMate 3000 RSLCnano
System with ProFlow Technology
Quick Installation Guide
Rev. 1.0
February 2016
© Thermo Fisher Scientific
Doc. No. 4820.4105
All rights reserved.
Hystar is a trademark of Bruker Daltonics. Empower is a trademark of Waters Corp. Microsoft, Windows is a
registered trademark of Microsoft Corp. All other trademarks are property of Thermo Fisher Scientific Inc. and
its subsidiaries.
Table of Contents
1. General Information
2
2. Software Installation to Support ProFlow Technology
3
3. Firmware Verification and Upgrade
4
4. Installation of the ProFlow Flow Meter
6
5. Instrument Configuration with the ProFlow Flow Meter 9
6. ProFlow Flow Meter Purging and Calibration
13
7. Supporting Information
19
Page 1
1. General Information
The ProFlow™ flow meter is designed for Thermo Scientific™ Dionex™
UltiMate™ 3000 RSLCnano systems equipped with NCS-3500RS or NCP-3200RS
modules. Follow the steps given below to change from a Classic flow meter to the
ProFlow flow meter.
General Installation Steps
• Upgrade Chromeleon™ Chromatography Data System/ SII software
• Upgrade firmware as necessary
• Turn off the NCx pump
• Exchange the Classic flow meter with the ProFlow flow meter
• Exchange the capillaries and connections
• Install the solvent shut off valves and elbow connector solvent lines
• Turn on the NCx pump
• Re-configure (delete/add) the NCx-3x00RS module in the Chromeleon / SII
Instrument configuration
• Purge and calibrate the system
• Run the Detailed Leak Test for the NCx pump to check flow connections are
tight
Parts required to upgrade NCS-3500RS or NCP-3200RS modules
Description
Part No.
ProFlow flow meter, with thermal flow sensors for nano LC
6041.7850
Upgrade kit for ProFlow flow meter
Including accessories required to upgrade an NCS-3500RS or NCP3200RS module with the ProFlow flow meter:
Solvent lines, elbow tubes, shut-off valves
Capillaries for the NC pump (nanoViper, MP35N)
Chromeleon 7.2 SR4 or later software DVD
SII for Xcalibur™ software DVD
Fitting plugs, Viper
6041.3003
Note:
•
•
Firmware 1.40 or later is required to operate the ProFlow flow meter with
NCx-3x00RS modules.
Thermo Standard Instrument Integration (SII) for Xcalibur 1.2 and DCMSLink
for Xcalibur can coexist on one PC.
Chromeleon 6.80 SR15b or later is needed to control RSLCnano systems
with ProFlow technology (this is not included in the upgrade kit).
Page 2
2. Software Installation to Support ProFlow Technology
Note: Install the software in accordance with the installation guidelines for the relevant
software package and the operation instructions for the respective NCx-3x00RS
module.
2.1
LC Setup
The following full Chromeleon license software versions are available
•
Chromeleon 6.80 SR15b or later
•
Chromeleon 7.2 SR4 or later
2.2
LCMS Setup
For LC-MS installations with the ProFlow flow meter and Thermo Scientific Mass
Spectrometry (MS) instruments, the following software options are available:
•
SII 1.2 for Xcalibur or later (an upgrade with Chromeleon 7.2 SR4 or later is
required)
Note: SII can only be used for Thermo Scientific MS detectors which operate
under Thermo Scientific Foundation 3.x or later.
Note: DCMSLink for Xcalibur does not support the ProFlow flow meter and
cannot be used to control an UltiMate 3000 RSLCnano system fitted with
ProFlow technology which is coupled to a Thermo Scientific MS detector.
•
Chromeleon 7.2 SR4 or later for UltiMate 3000 RSLCnano systems coupled
with:
o
o
o
o
o
Thermo Scientific Exactive series instruments
ISQ™ single quadrupole instruments
TSQ 8000™ triple-quadrupole GC/MS instruments
TSQ Endura™ quadrupole MS instruments
TSQ Quantiva™ quadrupole MS instruments
Note: The Thermo Scientific MS detector is directly controlled by Chromeleon in
contrast to SII for Xcalibur.
Note: Please check the list of supported hardware options in the software
release notes or Chromeleon Help. A Chromeleon license is required to control
the LC-MS system. Check the compatibility of Thermo Foundation and Xcalibur
versions and Chromeleon 7.x for LC-MS control.
•
DCMSLink 2.15 for HyStar™ or later
•
DII for Empower™ 2.14 or later
Page 3
3. Firmware Verification and Upgrade
Note: Software screenshots and examples below show the system configuration in
Chromeleon 7 or SII.
1. Start the Instrument Configuration Manager under Thermo Chromeleon 7 in the
Windows® Start menu.
2. Click Add Instrument and create an Instrument Name. If an existing instrument
configuration is to be modified to accommodate the ProFlow flow meter, go to the
next step.
Fig. 1: New instrument name window in Chromeleon Instrument Configuration
Manager
3. Add (or delete and add for existing Instrument configuration) the relevant NCx3x00RS module to the instrument configuration.
Fig. 2: The Add module to instrument dialog in the Chromeleon Instrument
Configuration Manager
Page 4
4. Verify that the correct firmware version is installed in the Messages Instrument
Controller (Audit Trail) of the Instrument Configuration Manager. If the firmware
version is 1.40 or later, proceed to step 6.
Fig. 3: Instrument configuration manager with the added NCS-3500RS module
5. Open the NCx Configuration window and go to the General tab. Select firmware
version 1.40 or later from the dropdown menu and click on Install to upload the
firmware. Wait until the module reboots and the Audit Trail reports successful
completion of the firmware download before proceeding.
Fig. 4: The General tab for the Pump Device showing the firmware update
6. Save the modified configuration to the Instrument controller (server).
Page 5
4. Installation of the ProFlow Flow Meter
Important:
To avoid damage from electrostatic discharge, wear appropriate
earthing protection during the replacement procedure.
1. Turn off the NCx-3x00RS pump module by switching off the power switch on the
rear of the module.
2. Disconnect the capillaries connected to the flow meter inlets and outlet.
Fig. 5: NC pump with Classic flow meter (detailed view)
No.
Description
1
Leak sensor
2
Rear seal wash system detector
3
Peristaltic pump
4
Purge screw
5
Flow meter inlet
6
Pump lights (hidden by the front panel door in this figure)
7
Connector for the pressure transducer cable (working pressure of the pump head)
8
Flow meter outlet
9
Purge outlet
10
Pump head
3. Unplug the pressure transducer cables.
Page 6
4. Loosen the two screws that attach the flow meter and pull the flow meter out toward
the front.
5. Install the ProFlow flow meter.
Note: Be careful not to pinch any tubing.
6. Fasten the attaching screws on the flow meter and plug in the pressure transducer
cables.
7. Exchange the capillaries and U tubes on the NC pump, with the capillaries from the
upgrade kit. This includes:
− Capillaries from the working cylinder to the equilibration cylinder (U-tube) on both
pump heads of the NC pump.
Fig. 6: U-tube for the NC pump of NCx-3x00RS module
− The capillary from the right pump head to the flow meter inlet.
− The capillary from the left pump head to the flow meter inlet
Fig. 7: Capillary from the pump head to the flow meter inlet
Tip:
The new capillaries can also be used with a Classic flow meter.
Page 7
8. Install the solvent shut off valves and elbow pieces on solvent lines A and B.
Fig. 8: Solvent shut-off valve and example schematic of an elbow piece
Tip: With the ProFlow flow meter, the shut off valves are required during the
Zero Balance Adjustment. The shut off valves allow the solvent flow
through the system to be shut off at the solvent reservoir, for example, to
prevent solvent from flowing through the system when a pump flow of zero
is required for a long period, such as during calibration.
9. Turn on the module by pressing the power switch on the rear of the module.
Fig. 9: Detail view of NCS-3500RS pump module with installed ProFlow flow meter
Page 8
5. Instrument Configuration with the ProFlow Flow Meter
5.1
System Configuration
1.
Start the Instrument configuration manager under Thermo Chromeleon 7 in
the Windows Start menu.
2.
Remove the NCx-3x00RS pump module from the configuration and then add
it again. This will ensure that all new features are available for the ProFlow
flow meter.
3.
Verify the following settings:
− The flow meter type (flow meter tab) is set to ProFlow.
− The solvent lists show the predefined solvents (Water, ACN_100%,
ACN_80%, MeOH).
− The signals page shows the signal channels for the ProFlow flow meter
as Enabled (NC_Pump_Flow, NC_Pump_Flow_LeftBlk,
NC_Pump_Flow_RightBlk).
Fig. 10: NCS-3500RS module configuration window with the Flowmeter tab active in the
Chromeleon Instrument Configuration Manager
Page 9
4.
Customize the configuration of your module with the ProFlow flow meter as
required.
Fig. 11: NCS-3500RS module configuration window with active Oven/Valves tab in
the Chromeleon Instrument Configuration Manager
5.
Add the other relevant modules of your RSLCnano system to reflect the
installed hardware.
6.
Save the modified configuration to the Instrument controller (server).
Page 10
5.2
Client Access in SII (no LC-MS control)
If Chromeleon is used for LC-MS control, proceed to section 5.3, page 12.
1.
Start the instrument configuration under the Thermo Foundation 3.x folder in
the Windows Start menu and add SII for Xcalibur to Configured Devices.
Fig. 12: Thermo Foundation Instrument Configuration window
The image above shows the Thermo Foundation Instrument Configuration
window: Left side displays installed drivers (available devices) for DCMSlink
for Xcalibur, Thermo Scientific SII, VelosTM Pro mass spectrometer and Q
ExactiveTM – OrbitrapTM mass spectrometer; right side displays the devices
configured for operation.
2.
Click on the Configure button.
Page 11
3.
Select the configured RSLCnano system with ProFlow flow meter.
Fig. 13: SII for Xcalibur Configuration window with selected RSLCnano system
4.
Open Xcalibur in the Thermo Xcalibur folder in the Windows Start menu.
5.
Click on the Direct Control button to open the ePanels for direct control of
the RSLCnano system.
Fig. 14: Xcalibur window segment with the configured RSLCnano system and Direct
Control button to access the Chromeleon ePanels
6.
Select the NCx-3x00RS Pump ePanel in the Chromeleon Console.
5.3
Client Access in Chromeleon
1.
Start Chromeleon 7 in Thermo Chromeleon 7 folder in Windows Start menu.
2.
Click on Instruments in Category Bars (lower left corner in the Console).
3.
Select the NCx-3x00RS Pump ePanel.
Page 12
6. ProFlow Flow Meter Purging and Calibration
1. Purge both pump heads of the NC pump for about 15 minutes.
Fig. 15: NC_pump segment of NCx-3x00RS ePanel with installed ProFlow flow meter
Note: Follow the instructions in the Ready Check Results window and open
both purge screws.
Tip: The partial flows for channel A and B are displayed along with the
total flow on the NC_Pump segment of the ePanel.
Fig. 16: A part of the NC_pump segment of an NCx-3x00RS ePanel
Page 13
Fig. 17: Ready Check Results window appears after the NC pump blocks purge is
executed
2. After purging the pump heads, purge the ProFlow flow meter for about 10 minutes.
Note: Follow the instructions in the Ready Check Results window and close both
purge screws.
Fig. 18: Ready Check Results window appears after the flow meter purge function is
executed
Page 14
3. Start the Adjust Zero Balance test on the NC Pump Wellness tab. A wizard will
guide you through the procedure.
Fig. 19: Wellness window in NC_pump segment and Zero Balance wizard
Page 15
4. Set the solvent types for the left and right block to match your application in the
More Options tab of the NC_pump group of NCx-3x00RS ePanel.
Fig. 20: More Options window of the NC_Pump segment in the NCx-3x00RS module
ePanel
Tip:
The ProFlow flow meter comes pre-calibrated for the four standard
solvents: Water, 100% Acetonitrile, 80% Acetonitrile with 20% Water,
and 100% methanol (MeOH). The calibration values for these solvents
are valid for the entire life time of the ProFlow flow meter. A recalibration of the solvent types is not necessary if the ProFlow flow
meter is removed from the system and installed in another NCx-3x00RS
pump module.
Tip:
Separate solvent calibrations are not required with the addition of up to
2% of a solvent additive (e.g. for 2% acetonitrile / 98% water, the solvent
type ‘_Water’ can be used. Similarly, small amounts of phase modifiers
(<1%) e.g TFA, FA do not need to be taken into account for the solvent
calibration.
Tip:
A solvent calibration is only necessary for a solvent composition that
differ significantly from pre-calibrated standard solvents and has not
previously been calibrated for the ProFlow flow meter. Once a solvent
type calibration has been made, the solvent calibration is valid for the
lifetime of the ProFlow flow meter. Click Solvent calibration on the
Wellness tab in NC_pump section of NCx-3x00RS module to access a
Wizard that will guide you through the procedure. The procedure will
take around 1 hour and will require manipulation with fluidics.
Page 16
5. Calibrate the working piston pressure transducers of the left and right blocks as
follows:
−
Click on the Wellness tab In the NC_pump group.
−
Select either the left of the right NC pump block one of the Calibrate Work.
Press. Transducer buttons to start the procedure.
−
A Ready Check message is displayed.
Fig. 21: Ready check results window appeared after commencing the working
pressure transducer calibration
−
Close the flow meter outlet with the Viper plug.
−
Close the purge valves.
−
Press Execute despite warnings.
Tip:
Successful completion of the WP calibration will be reported on
the instrument display panel and in the Chromeleon Audit Trail.
Fig. 22: The message that shows the result of Working Pressure transducer
calibration on the NCx-3x00RS module
Fig. 23: Audit trail zoom in that confirms successful calibration of working pressure
transducers
Page 17
6. Repeat the Calibrate Work. Press. Transducer routine for the second block (repeat
the procedure described above).
7. Perform a Detailed Leak Test to check that all NC_Pump flow connections are tight.
−
Click on the Wellness tab in the NC_pump group.
−
Select the Detailed Leak Test and follow the instructions given in the wizard.
The system is now ready for operation!
Page 18
7. Supporting Information
Documents that are available for further reference.
•
•
•
•
•
•
Operating Instructions for the UltiMate 3000 modules
Installation Qualification manual for installation of the UltiMate 3000
RSLCnano system
RSLCnano Standard Applications Manual
Viper™ Capillaries and Finger Tight Fitting System Installation and Operation
Guide
Nano Connector for UltiMate 3000 RSLCnano Systems Installation
Instructions
Application/technical notes provided on the Thermo Scientific website
Table 1. Spare Parts
Item
Part No.
Quantity
in the kit
Upgrade kit for ProFlow flow meter, including
6041.3003
Capillary, nanoViper, MP35N (0.25 x 155 mm I.D. x L)
To be used, for example, for calibrating solvents with
the ProFlow flow meter or establishing the solvent
viscosity with the Classic flow meter or for purging the
flow meter.
6041.7892
1
Capillary from working cylinder to equilibration
cylinder (U-tube)
6041.7888
2
Capillary from left pump block to flow meter inlet
6041.7890
1
Capillary from right pump block to flow meter inlet
6041.7893
1
6040.2303
2
Solvent shut off valve
2265.0010
2
PTFE elbow tubing piece 1.0x3.0x100mm ID x AD x L
6040.8122
2
SII for Xcalibur Service Release DVD
4780.0384
1
Chromeleon 6.80 Service Release DVD*
4580.0316
1
Chromeleon 7 Service Release DVD
4580.0720
1
NCS Pump head Capillaries
Viper Fitting Plugs
Fitting plug (Viper, titanium, biocompatible)
Solvent Shutoff Valves
Software
*This item is not included as part of the upgrade kit.
Page 19
Table 2. nanoViper tubing matrix
I.D.[Color code] and corresponding part number
Length
(mm)
20 µm
[Orange]
50 µm
[Brown]
75 µm
[Black]
100 µm
[Red]
150 µm
[Purple]
70
6041.5120
6041.5123
6041.5126
6041.5810
6041.5817
150
6041.5121
6041.5124
6041.5127
6041.5811
6041.5818
250
-
-
6041.5730
6041.5812
6041.5819
350
6041.5240
6041.5540
6041.5735
6041.5813
6041.5820
450
-
-
-
6041.5814
6041.5821
550
6041.5260
6041.5560
6041.5760
6041.5815
6041.5822
650
6041.5275
6041.5575
6041.5775
-
-
750
6041.5280
6041.5580
6041.5780
6041.5816
6041.5823
950
6041.5122
6041.5125
6041.5128
-
-
Page 20