ANNUAL REPORT MARCH 2009 – FEBRUARY 2010
Transcription
ANNUAL REPORT MARCH 2009 – FEBRUARY 2010
An Bè Jigi1: Use of Local Diversity for Enhancing Nutrition (especially Fe/Zn bioavailability) in sorghum/pearl millet consuming communities ANNUAL REPORT MARCH 2009 – FEBRUARY 2010 Researchers: • • • • • • Marjon Tuinsma, Country Director, HKI Mali Letitia SAM, Country deputy Director Programs, HKI Mali Zoumana Berthe, SAN+ Deputy Coordinator, HKI Mali Coulibaly Salimata Sidibé, LTA, IER Fred Rattunde, Principal Researcher, ICRISAT, Mali Vera LUGUTUAH, ICRISAT,Mali. Funded by : 1 An Bè Jigui: <<Hope for all>> in Bambara language TABLE OF CONTENTS LIST OF ACRONYMS __________________________________________________ 3 SUMMARY____________________________________________________________ 4 1. BACKGROUND AND RATIONALE ___________________________________ 5 2. PROJECT BACKGROUND __________________________________________ 7 3. PROJECT OBJECTIVES AND ACTIVITIES PLANNED _________________ 8 4. RESULTS OF THE FOURTH PROJECT YEAR ________________________ 11 4.1 Culinary tests __________________________________________________________ 11 4.2 Micronutrient Testing of Sorghum Varieties ________________________________ 11 4.3 Analyses of Decortication Yields: Culinary Test______________________________ 12 4.4 Analysis of Fe, Zn and Phytate in whole- and decorticated grains: Culinary Test __ 14 4.5 Analyses of Fe and Zn content of On-Farm Participatory Variety Trial samples ___ 16 4.6 Training of trainers on transformation techniques that assure bioavailability of iron and zinc in meals __________________________________________________________ 20 4.6.1 Millet malting technique ______________________________________________________ 20 4.6.2 Enriched flours _____________________________________________________________ 21 4.6.3 Soya soumbala production ____________________________________________________ 21 4.6.4 Soya milk preparation ________________________________________________________ 22 4.7 Training manual development ____________________________________________ 22 4.8 Nutrition improved meals by adding Moringa Oleifera and Okra Baobab leaves. _ 22 4.9 Training of trainers to conduct group discussion _____________________________ 25 4.10 The production and broadcasting of radio programs on local radio the 3 sites ___ 25 4.11 The Development of M&E guideline for field agents _________________________ 26 4.12 The production of educational materials for field agents ______________________ 26 4.13 Theater as a communication and education tool _____________________________ 26 4.14 Participation in CoP____________________________________________________ 26 4.15 The organization of a final nutrition survey (using KAP method) ______________ 26 5. TEAM REPORT SECTION ___________________________________________ 27 5.1 Integrated activities _____________________________________________________ 27 5.2 Insights and lessons learned ______________________________________________ 28 5.3. Conclusion and the way forward __________________________________________ 28 6. FINANCIAL REPORT SECTION ______________________________________ 29 7. WORKPLAN FOR MARCH AND APRIL 2010 (No-cost extension) ___________ 29 ANNEX 1 Four-year workplan ___________________________________________ 30 ANNEX 2 Succes stories or most significant change. _________________________ 38 Annex 3. Training materials Transformation _______________________________ 40 2 LIST OF ACRONYMS ABJ ACOD AOPP BCC CCRP CD ECOWAS EDSM FAO FEWSNet FY GAA GIS HKI ICRISAT IEC IER IFA IMR ITA KPC L LTA M&E MOH MOU MRTC NGO PVO TADD ULPC UN UNICEF USAID VA VAC VAD WA WCBA An Bè Jigi Association Conseil pour le Développement Association des Organizations Professionnelles Paysannes Behavior Change Communication Collaborative Crops Research Program Country Director Economic Community of West African States Étude Démographique et de la Santé au Mali Food and Agriculture Organization Famine Early Warning System Network (USAID-funded project) Fiscal Year German Agro Action Geographic Information System Helen Keller International International Crops Research Centre for the Semi-Arid Tropics Information Education Communication Institut d’Economie Rurale iron+folic acid Infant Mortality Rate Institut de Technologie Alimentaire Knowledge, Practice and Coverage (Survey) Leveraged Laboratoire de Technologie Alimentaire Monitoring and Evaluation Ministry of Health Memorandum of Understanding Malaria Research and Training Centre Non-Governmental Organization Private Voluntary Organization Tangential Abrasive Dehuller Device Local Union of Cereal Producers (Union Locale des Producteurs de Céréales) United Nations United Nations Children’s Fund United States Agency For International Development Vitamin A Vitamin A Capsule Vitamin A Deficiency West Africa Women of Child Bearing Age 3 SUMMARY The An Bè Jigi project (Hope for all in the local language) received its first year funding at the beginning of March 2006. The project has been implemented by three key partners: Helen Keller International (HKI), Institute of Rural Economy (IER) and the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) who have collaborated closely with several more partners: German Agro Action, Association Conseil pour le Développement ACOD, Local Union of Cereal Producers ULPC, Malaria Research and Training Centre MRTC). HKI is the project coordinating agency. The project is ending after 4 four successful years of accomplishments in the area of nutrition. The general objective of the project was to: enhance nutrition through sustainable bio fortification of sorghum and millet using participatory variety development. The key target groups were: children under 5 years of age and women of child bearing age (WCA). The ABJ project works in synergy with the ALIVE project on legume intensification and participatory variety enhancement (joint baseline survey, organization of planning meeting) and the Seed Systems project (seed production). The last years of the implementation of project activities have seen the continuation of crops research particularly on the bioavailability of iron and zinc in different varieties of millet and sorghum in the three zones of the project. Samples were sent to laboratory in Mali and in India for the identification of millet and sorghum varieties. The level of acceptability and uptake by the communities were also tested in the three interventions zone. The HKI team organized several workshops with trainers of trainers and women groups on improved Nutrition, using the newly developed Picture Book Aid/Image Box. The IER team also organized training sessions for trainers in several cereal transformation techniques and utilisation of Moringa and Baobab leaves in recipes. The present report describes in detail progress and findings achieved by the AN BE JIGI team. While the project achieved much progress, there were also weaknesses identified or areas where more research would be needed. The activities during the 4rd year of the project have been conducted well in general. However some activities have known a delay due to availability of staff and/or consultants. A no-cost extension until the end of April has been approved by the McKnight foundation to enable the AnBeJigi team to finalize all planned activities of the project. Therefore a final report will be submitted by the end of May 2010. 4 1. BACKGROUND AND RATIONALE Mali ranks 173th out of 177 countries in the Human Development Index2. Malnutrition prevalence is high (34% of children under five are stunted, 32% underweight and 13% are wasted) and is the single most important cause of child mortality (more than 50% of deaths)3. Furthermore, only 32% of children less than six months are exclusively breastfed and 30% of children between 6-9 months receive complementary feeding. Most Malian children 6 to 59 months of age are anemic (82%) with iron deficiency as the most probable cause. Evidence from another West African country indicates that approximately half of anemia is due to iron deficiency.4 Women of reproductive age are anemic (63%), the frequency being even higher among pregnant women (73%)5. It is estimated that 33% of the world population lives in countries with high risk of zinc deficiency (including Mali). In developing countries, zinc deficiency ranks 5th among the leading 10 risk factors for disease; WHO attributes 800,000 deaths worldwide each year to zinc deficiency and the loss of over 28 million healthy life years6. Rates of iron deficiency anemia may be used as suggestive evidence of the risk of zinc deficiency7. Micronutrient deficiencies may even be increasing in sorghum/millet producing zones of West Africa (WA) with a population doubling every 30 years8, and with decreasing access to the best source of bioavailable iron - meat. Expansion of agricultural activities, intense hunting and decreased wildlife populations have decreased wild game in diets. Also, per capita availability of animal products is reduced due to human population growth, and previous droughts reducing livestock numbers (small ruminants are only now reaching levels that existed prior to the droughts of the 1980s, and large ruminant populations are still recovering). Thus, populations in semi-arid West Africa are increasingly dependent on plant sources for micronutrient sufficiency, even though these sources have much lower bioavailable iron and zinc. New, sustainable sources or techniques of providing required levels of bioavailable micronutrients are necessary for human development, productivity, and well-being in the region. These sources need to be affordable, and locally available. Thus the challenge is to improve utilization of local diversity to meet nutritional needs. Sorghum and pearl millet constitute the main staple foods for over 100 million people living in the semi-arid tropics of West and Central Africa. These crops are also an important source of micronutrients in this region, providing one third to one half of Fe/cap/day9. 2 UNDP 2007 Human Development Index EDS IV 2006 4 Asobayire et al, 2001. Prevalence of iron deficiency anemia with and without concurrent anemia in population groups with high prevalence of malaria and other infections: a study in Cote d’Ivoire. Am. J. Clin. Nutr. 2001; 74: 776-82 5 M/DHS, 2001 6 International Zinc Association, 2004 Zinc for Better Health 7 IZiNCG, 2004, Technical document #1. Food and Nutrition Bulletin vol. 25 no 1, supplement 2 8 UN Population Division, 1997 (web site paper) 9 FAO Yearbook. Per capita availability of various nutrients, extent of iron deficiency, and the per capita availability of iron through various crops in Africa and Latin America and HarvestPlus Workshop, 2001 3 5 Mineral components of sorghum grain vary widely10. Varietal diversity is important, with for example contents in whole grains of 99 cultivated sorghum varieties range from 4.714.1 mg/100g Fe and 2.5-6.8 mg/100g Zn11. Sorghum and millet are indigenous, with WA being the center of origin and diversity, and large genetic diversity exists for most traits of agronomic importance. Initial research on exploring diversity of WA sorghum/millets for micronutrient contents with HarvestPlus12 is ongoing but work has been limited to research station studies examining whole grain. In order to achieve impact, it became necessary to examine nutrient availability at actual farmers’ field level, and in grain products actually consumed. Some of the issues were that: • grain mineral content vary greatly depending on mineral composition of soils and conditions under which varieties are grown13; • grain decortication (removal of the outer layer) prior to producing flour or grits for actual food preparation. As the majority of minerals occur in the outer layers (69% germ, 11% in seed coat) major amounts of Fe/Zn may be lost even before the food is prepared. Differences between varieties have been observed for decortication losses and ease of decortication. Yet to date ,there have been no systematic efforts to understand varietal differences for decortication traits, nor methods available to exploit these differences to improve Fe/ Zn availabilitye. • Antinutritional factors in grains, such as phytate, can bind Fe/Zn and may have larger consequences on bioavailability of Fe/Zn than content per se. There is limited information on genetic diversity for phytate contents in decorticated grains. 10 Hulse et al. 1980. Sorghum and the Millets: their composition and nutritive value. Academic Press, New York. 11 Subramanian V and Jambunathan V. 1984. Chemical composition and food quality of sorghum. Pages 32-47 in Nutritional and processing quality of sorghum (Salunkhe DK, Chavan JK and Jadhav SJ, eds.). Oxford & IBH Publishing Co., New Delhi. 12 HarvestPlus is an international, interdisciplinary, research program that seeks to reduce micronutrient malnutrition by harnessing the powers of agriculture and nutrition research to breed nutrient dense staple foods. www.harvestplus.org 13 Klopfenstein, C.F. and r. C. Hoseney. 1991. Nutritional properties of sorghum and the millets. In Sorghum and Millets: Chemistry and Technology, ed. David A.V. Dendy, pg. 125-168 6 2. PROJECT BACKGROUND An Be Jigi (ABJ) is a project funded by the McKnight Foundation’s Collaborative Crops Research Program (CCRP). The project was funded for four years for the amount of 420,000$. The aim of the project is to bring agricultural research and nutrition aspects closer. The principal partners in the consortium are Helen Keller International (HKI), International Crops Research Institute of the Semi-Arid Tropics (ICRISAT) and Institut d’Economie Rurale (IER) with HKI as the principal partner. Aside from the three main partners, the project works in collaboration with German Agro Action (AAA) in the northern region (Tenenkou), Association Conseil pour le Developpement (ACOD) in the Mande zone and Union Locale des Producteurs de Cereales (ULPC) in Dioila. . Table 1 shows the respective roles of the ABJ partners. Table 1: Roles of ABJ project partners ORGANIZATION ROLE HKI Coordination and technical nutrition assistance ICRISAT Genetical research and integrated natural resource management IER Agronomical research and food technology GAA/ACOD/ULPC Participatory field work with farmers on site 7 3. PROJECT OBJECTIVES AND ACTIVITIES PLANNED The overall goal of the project is to: Enhance health, productivity, well-being through improved nutrition. The general objective is to: Enhance nutrition through sustainable biofortification of sorghum and millet using participatory variety development Key target groups are: Children under 5 years of age and women of child bearing age (WCA). The Key outputs over the 4 year life span were: 1) The enhancement of the nutritional contribution of staple cereal grains by tapping available diversity • The selection of methodology which integrates nutrition into participatory breeding • Superior farmer preferred varieties 2) The identification of critical determinants/opportunities for improved nutrition within local diet and locally grown foods • Identification of key nutritional patterns, feeding practices and beliefs that determine nutritional status • Identification of nutrient dense foods, enhancers and inhibitors (dark green leafy vegetables, legumes) • Understanding of critical determinants on Fe/Zn bioavailability (processing, cooking, feeding and hygiene practices, environmental sanitation). 3) The formulation, testing and promotion of combinations of practices that assure synergy and, sustainability • Promising methods formulated • Testing/documentation/promotion of most promising methods and practices • Community Education materials developed • Models/methods/guides available for development actors (research and development) to incorporate nutrition The table below summarizes the overall planned activities during the project lifespan. Table 2: planned activities for the ABJ project YEAR PLANNED ACTIVITIES Year 1 Start up workshop, collection of required basic information on grains, village nutrition surveys (practices/knowledge/attitudes/resources available), input of expertise, development of methods, and strengthen capacity of IER for analysis. Obtain basic information about sorghum and millet grain traits necessary to establish effective, feasible selection methods for improved nutritional value. Year 2 – 3 Participatory Learning and Action: iterative process of Assessment, Analysis enabling identification of key interventions, and Action (Triple A), supervision and monitoring. A 8 Year 4 Year 5 selection protocol is elaborated and tested, participatory breeding that incorporates key grain traits for nutrition, development of IEC materials Endline survey, nutrition impact assessment, preparation of materials/guides for educating community of practice members, for target populations in common agroecological zone, for NGO/Research organizations operating in common zones. Model for region: ways for scaling up and out. ABJ partners will continue to assess sustainable adoption of behavioral change after the project has ended through leveraged resources Activities are implemented in Mali. The point of entry was the already existing and strong participatory sorghum/millet variety development partnerships in two contrasting, agroecological and cultural zones of importance for each staple cereal crop. Activities in sorghum-based systems were conducted in the Dioila district, 160km east of Bamako, and the Mandé district 60km southwest of Bamako. Whereas the Dioila district is more commercially oriented, with cotton production and mechanization being much more advanced, the Mandé district is less market oriented, and agricultural intensification and cotton production is proceeding at slower pace. The farming systems are cereal (and cotton) based, with primarily pure-stand cultures of sorghum, with pearl millet and increasingly maize as secondary cereal crops (and rice in low-lands). Groundnut is the predominant legume crop, but its area of production is markedly lower than that of cereals. Both zones represent the main “Savanah sorghum belt” that extends from Senegal across Mali, and Burkina Faso. Activities in millet-based systems are being conducted in the more northerly Tenenkou Cercle (Mopti Region) (400mm rainfall), which is thinly populated (Bambara and Tamachek ethnic groups). Tenenkou provides a sample of the ecological, social and infrastructure conditions important in the Sahelian zone that extends from Northern Senegal, across Mali, northern Burkina Faso and Niger. (see map below for the 3 sites). 9 Zone of ABJ Project This project is based on the vision of integrating research and development, agriculture and nutrition, scientists and farmers, corresponding to the concept of Participatory Learning and Action. The analyses of key constraints and positive deviance will provide the basis for applied interventions. 10 4. RESULTS OF THE FOURTH PROJECT YEAR Activities for Year four were conducted in collaboration with all consortium partners. The Partner organization with expertise in a given area was the lead in the implementation of the activity providing an opportunity to learn from each other. 4.1 Culinary tests The Culinary test was conducted in a total of 4 villages in March 2009. A total of 15 varieties and four local checks were tested by a total of 25 participants in each village involved in the “blind” taste-testing. The test had three replications, with three grain samples that were each decorticated, milled and cooked by a separate group of women. Two varieties, Oki and Kakou, were rejected due to the poor consistency of To, and the excess bran leading to lower “food yield”. The culinary tests of the 2009 Participatory Trials are being conducted from March 26 to April 2, 2010. 4.2 Micronutrient Testing of Sorghum Varieties A total of 1120 Grain samples of the 70 variety sorghum trials under low- and high-P conditions at IER-Kolombada and ICRISAT-Samanko from 2008 were evaluated for decortication yield and grain size and sent to ICRISAT-India for Fe and Zn analyses. This set of 70 varieties represents the diversity of varieties adapted to the Sudanian zone of West-Africa, and includes materials of differing racial backgrounds and corresponding grain types. The iron and zinc values were received in February 2010 and initial analyses of the 2008 grain samples were conducted. Combined analyses of the three year data set (2006, 2007 and 2008) is underway to a) characterize these varieties for micronutrient content and identify varieties and source materials with superior nutritional value, b) assess genotypic and environmental sources of variation for Fe and Zn contents, and c) assess genotype x environment interactions and relationships among grain traits to guide designing of a selection methodology for biofortified sorghum variety development. Grain samples of the Tall-variety and the Short-variety Participatory Trial of 2008 were collected from all 14 participating farmers. The 900 samples (32 varieties, 14 farmers, 2 reps per farmer) were assessed for mechanical decortication yields and sent for Fe and Zn analyses. The knowledge that grain produced in women’s sorghum fields is more targeted for feeding children in the “fourth meal” offers a potential pathway for delivering micronutrient rich varieties to children’s plates. A total of 35 “3 Variety” trials were conducted by women farmers in 2009 to begin assessing this potential pathway and the actual micronutrient levels in grains produced under the low-fertility conditions of women’s fields. The trials included the variety Tieble which shows promise of having higher iron and zinc content in the grain. Data on varietal performance and women’s varietal preferences were collected and are being entered into a database. Grain 11 samples obtained from 15 trials are currently being assessed for decortication yield and prepared for Fe and Zn analyses. The costs for laboratory analyses of Fe and Zn are shared with the European Union INSTAPA Project. The INSTAPA project also provides support for the scientific staff time contributing to the work reported here. Statistical analyses: The assistance of Roger Stern and the Statistical Services Centre of Reading University was invaluable in helping us progress on data analysis in a way that answers the most pertinent research questions. This work has both provided more clarity of our results as well as strengthened capacity for continuing the analytical work. We present in this report some of the current analyses. Although some of this data has already been presented, the nature and clarity of the findings warrant being recorded. 4.3 Analyses of Decortication Yields: Culinary Test The 2006 and 2007 Culinary Tests data sets were analyzed to gain insights into the key sources of variation for decortication yields, and the consequences for grain and micronutrient losses. The 2006 data set consists of four contrasting varieties cultivated in 9 villages, and the 2007 data set is based on five varieties grown in 8 villages. Varieties differed significantly for decortication yield with both the traditional manual decortication with wooden mortar and pestle and the mechanical decortication with the TADD (Tangential Abrasive Decortication Device) in 2006 as well as 2007 (Table 3). The differences for decortication yields between varieties were substantial, ranging from 60 to 87% in 2006 and 67 to 89% in 2007. These varietal differences were much greater than the differences between villages (Tables 4 and 5). Furthermore, the differences among varieties were quite stable over villages. The variety x village interactions for mechanical decortication yields can be explained by the inconsistency of varieties with the lowest decortication yields whereas varieties with high decortication yields were stable across villages. The mechanical decortication yields were generally lower but reasonably predictive of the manual decortication yield (Tables 6 and 7). However, two of the low decortication varieties, Kalaban and Dougouba, showed considerably reduced yields with mechanical decortication. This is likely due to the fact that mechanical decortication is for a constant period of time (4minutes) whereas manual decortication is stopped when women consider the grain sufficiently clean. However, since these varieties showed low decortication yields with both methods, this inconsistency may not seriously affect the choice of varieties with lower decortication losses (Table 3). Analysis of variance for decortication yield of both manually (MAN) and mechanically (TADD) decorticated grains for the 2006 and 2007 culinary test 12 Table 3. Analysis of variance for decortication yield of both manually (MAN) and mechanically (TADD) decorticated grains for the 2006 and 2007 culinary test Variety Village Variety*Village Decortication yield_ MAN F pr. <0.001 <0.001 0.46 2006 Decortication yield _TADD F pr. <0.001 <0.001 <0.001 2007 Decortication Decortication yield_ MAN yield _TADD F pr. F pr. <0.001 <0.001 0.003 <0.001 0.224 0.013 Table 4.Variety and village means of decortication yield (%) of mechanically (TADD) decorticated grains of the 2006 culinary test Village Seyla Tonga Teneya Kegnero Kafla Siby Magnambougou Siranikoro Seribila Mean Variety Tieble 86.0 87.7 87.6 90.7 87.2 85.1 87.2 88.0 87.8 87.5 Guana 79.6 79.3 75.9 82.6 77.4 78.0 73.0 70.4 76.9 77.0 Weli 64.2 80.6 74.2 76.4 62.6 65.7 61.6 57.7 66.1 67.7 Kalaban 79.3 59.9 65.1 52.9 66.2 61.1 60.0 56.5 41.5 60.3 Mean 77.3 76.9 75.7 75.6 73.3 72.5 70.5 68.2 68.1 73.1 Table 5. Variety and village means of decortication yield (%) of mechanically (TADD) decorticated grains for the 2007 culinary test Variety Village Kafla Kalague Keniero Kenié Seribila Siranikoro Teneya Wacoro Mean Tieble 88.3 90.9 89.7 87.7 89.5 88.9 90.3 86.9 89.0 Djelefi 89.2 90.6 88.1 87.9 90.3 88.6 87.3 87.7 88.7 Tamia 85.0 85.5 85.2 83.2 86.9 87.2 86.3 78.0 84.7 Tiandougou Coura 75.9 83.7 78.8 77.0 79.3 74.9 80.0 75.5 78.1 Dougouba 70.0 73.0 73.1 64.4 72.5 63.8 61.5 58.2 67.1 Mean 81.7 84.7 83.0 80.0 83.7 80.7 81.1 77.3 81.5 13 Table 6. Variety means of decortication yield of manually decorticated grains of the 2006 culinary test Variety Tieble Guana Kalaban Weli Mean Manual (%) 82.5 73.9 68.7 68.0 73.3 Mechanical (%) 87.5 77.0 60.3 67.7 73.1 Table 7: Variety means for decortication yield of manually and mechanically (TADD) decorticated grains of the 2007 culinary test Variety Tieble Djelefi Tamia Tiandougou Coura Dougouba Mean Manual (%) 77.2 72.9 69.5 Mechanical (%) 73.6 76.7 73.3 65.2 64.7 69.9 71.1 52.1 69.3 4.4 Analysis of Fe, Zn and Phytate in whole- and decorticated grains: Culinary Test Grain decortication causes major losses of both Fe and Zn, with only 40 to 60% of the original whole grain micronutrient contents still being retained after traditional manual decortication (Tables 8 and 9). Thus exploiting varietal differences for Fe and Zn retention or options for using whole grains could be of great importance for enhancing micronutrient nutrition. The percentage retention of Fe, Zn and phytate differed significantly by variety for both manual as well as mechanical decortication in 2006 and 2007 analyses (Table 10). The varietal differences for Fe and Zn retention were very stable over villages, with basically no significant variety by village interactions. Fe and Zn retention therefore can be effectively assessed without sampling over many villages. The actual magnitude of micronutrient retention after manual decortication did not differ substantially between varieties except for a few of the varieties that had the lowest decortication yields. Retention of Fe and Zn was generally lower with manual- relative to mechanical decortication (Tables 8 and 9). Estimates of Fe and Zn retention were inconsistent between manual and mechanical decortication for some varieties with lowest decortication yields. The varieties “Kalaban” and “Dougouba” had increased Fe retention with manual decortication, but did not have superior Fe contents of decorticated grain due to the low Fe content of their whole grains. Likewise for Zn, Kalaban had the lowest decorticated grain zinc content despite its higher Zn retention (Table 8). Therefore, assessing micronutrient contents of either decorticated grain or 14 whole grain seems to be more predictive of nutritional value than estimating micronutrient retention per se. Table 8. Variety means of phytate (IP6), Fe, Zn contents of whole grain (WG), manually (MAN) and mechanically (Mec) decorticated grains, and percent retention (Ret) in decorticated grain relative to whole grain for 2006 culinary test samples. IP6 WG Variety (ppm) Tieble 16 Guana 13 Weli 10 Kalaban 9 Mean 12 IP6 MAN (ppm) 9 7 5 5 6 Ret IP6 (%) 55 49 52 51 52 Ret Fe Fe Fe Fe WG MAN Mec Man (ppm) (ppm) (ppm) (%) 27 13 17 50 24 12 13 50 24 12 15 52 19 12 12 64 24 12 14 54 Ret Fe Mec (%) 64 57 62 65 62 Ret Zn Zn Zn Zn WG MAN Mec MAN (ppm) (ppm) (ppm) (%) 18 9 12 46 21 9 12 43 17 7 10 49 12 6 8 54 17 8 10 48 Ret Zn Mec (%) 69 58 60 68 64 Table 9. Variety means of phytate (IP6), Fe, Zn contents of whole grain (WG), manually (MAN) and mechanically (Mec) decorticated grains, and percent retention (Ret) in decorcticated grain relative to whole grain for 2007 culinary test samples. Ret IP6 IP6 Ret Fe Fe Fe Fe WG MAN IP6 WG MAN Mec Man (ppm) (ppm) (%) (ppm) (ppm) (ppm) (%) 8.6 2.7 32 37 15 25 41 7.7 2.0 26 38 14 26 39 7.4 2.7 36 37 15 23 42 Variety Tieble Djelefi Tamia Tiandougou Coura 7.1 Dougouba 8.3 Mean 7.8 2.0 3.0 2.5 29 38 32 32 36 36 12 15 14 20 19 22 38 44 41 Ret Fe Mec (%) 67 68 63 Ret Zn Zn Zn Zn WG MAN Mec MAN (ppm) (ppm) (ppm) (%) 20 9 15 44 21 7 16 36 17 8 13 46 Ret Zn Mec (%) 74 75 72 63 54 63 17 21 19 69 53 69 7 9 8 12 11 13 15 44 47 43 Table 10. Variety and village main effects and interactions for percent retention of Fe, Zn and phytate (IP6) after traditional manual (MAN) and mechanical (Mec) decortication. Year Fe_MAN Fe_Mec 2006 Zn_MAN Zn_Mec IP6_MAN Fe_MAN Fe_Mec 2007 Zn_MAN Zn_Mec IP6_MAN F pr. Variety 0.003 0.04 0.003 <0.001 0.17 0.18 <0.001 0.02 <0.001 <0.001 Village 0.001 0.36 0.14 0.07 0.21 <0.001 <0.001 <0.001 <0.001 <0.001 Variety*Village 0.27 0.14 0.46 0.24 0.06 0.29 <0.001 0.79 0.00 0.23 4.5 Analyses of Fe and Zn content of On-Farm Participatory Variety Trial samples The grain samples collected from the On-farm “Short Variety” and “Tall Variety” Participatory Sorghum Variety Trials provides an opportunity to assess the importance of varietal variation and variety by environment interaction for Fe and Zn contents over a larger sampling of both genotypes and environments. The Tall and Short Variety Trials were conducted by two farmers each in each of 8 villages in 2007 and 2008, with each farmer having 2 repetitions. The Tall and the Short trials each consisted of 16 varieties, which were the same in 2007 and 2008. The complete Fe and Zn analysis of 2007 samples was received and will be reported here. The first two villages of 2008 samples have also just been obtained. The Fe and Zn contents were estimated at the ICRISAT-Patancheru lab on mechanically (TADD) decorticated grains to minimize risks of contamination by soil14 and to relate as closely as possible to the food actually being consumed. Grains were decorticated for 4 minutes, using duplicate 20g samples. The Fe and Zn determinations were made as duplicate (2007) or single analysis with repeat of questionable samples (2008). Initial analyses by individual village indicate that the results were highly repeatable with relatively low error for Fe and Zn contents, as shown by the heritability estimates on entry-mean basis of the 2007 Short Variety Trial results (Table 11). Varieties differed significantly for both Fe and Zn contents over villages for the 2007 Short Variety Trial (Table 12). The differences among varieties were very large, with varietal means ranging from 15.5 to 25.0 ppm for Fe (Table 13) and from 10.1 to 15.3 ppm for Zn (Table 14). 14 Latunde-Dada, G.O. Contaminant iron in water and soil samples and in vitro availability studies. Food and Nutrition Bulletin. www.unu.edu/unupress/food/8f144e/8f144e0j.htm 16 Although farmers within village were also significant sources of variation, these variance components were relatively smaller than those for variety. There was a significant variety by village interaction for both Fe and Zn contents (Table 12), although the rank of varieties was relatively consistent across all villages. These results, based on a larger sample of varieties, provide further support of the major importance of varietal differences for Fe and Zn contents as was found in the Culinary Tests. The short plant height test varieties “Djelfi” and “Boulen” both had high Fe and Zn contents. These levels were comparable to that of the tall landrace check variety “Tieble” (Tables 13 and 14). These results suggest that stable varietal differences for Fe and Zn contents can be exploited under the complex and varied soil-, rainfall- and management practices of farmer’s field conditions. The micronutrient range of the short plant height test entries points to the importance of screening new breeding materials to avoid dissemination of exceedingly low micronutrient content varieties. The relationship between degree of Guinea-race genetic background with higher micronutrient contents is apparent in both the “Short” and “Tall” Variety Trial results of 2007 (Table 15). This result, if confirmed with additional data sets, will be critical for guiding future breeding work to enhance the micronutrient density of this staple cereal for West Africa. These analyses are on-going. Table 11. Heritability estimates for Fe and Zinc of “Short Variety Trial” by village in 2007 Village Kalague Teneya Wacoro Keniero Kenié Kafla Seribila Heritability Fe_TADD 0.64 0.67 0.85 0.88 0.88 0.90 0.94 Siranikoro 0.95 Mean 0.84 for Heritability Zn_TADD 0.78 0.77 0.90 0.86 0.80 0.83 1.00 for 0.92 0.86 Table 12. Variance components and respective standard errors for Fe and Zn contents of mechanically decorticated grains of the 2007 Participatory Short Variety Trial. Fe VarComp Village 1.56 Variety 8.72 Village/Paysan 1.87 Village*Variety 4.25 (Village/Paysan)*Variety 0.91 s.e. 1.64 3.48 1.07 0.92 0.61 Zn VarComp 0.918 2.769 0.583 0.693 0.424 s.e. 0.716 1.077 0.338 0.221 0.216 17 Table 13. Mean Fe contents (ppm) of mechanically decorticated grains of 16 varieties from the 2007 Short-Variety Trial, averaged over two farmers per village. Variety Djelefi Tieble Boulen Tamia Oki Tieba Marakanio Tiandougou Coura Lebo Kouladji Gagna Koule Kakou Grinkan Tiguila Drasa Mean Seribila 24 24 23 24 20 18 16 Siranikoro 26 25 23 25 20 19 17 Kenié 26 25 27 23 24 23 19 Wacoro 25 23 27 19 26 23 20 Kalague 23 25 23 22 23 22 24 Keniero 27 26 23 24 21 22 22 Teneya 23 24 25 22 24 25 24 Kafla 25 27 26 24 24 24 24 Mean 25.0 24.9 24.8 22.9 22.7 21.8 20.6 24 18 18 16 14 15 15 15 14 18.5 20 18 17 16 16 16 16 15 15 19.1 19 18 18 19 18 15 17 17 15 20.1 18 19 19 18 18 21 18 18 15 20.3 16 20 21 18 21 19 17 17 16 20.5 22 20 18 18 21 19 18 16 16 20.9 19 21 24 20 19 20 21 19 17 21.7 19 22 21 20 19 20 18 19 17 21.8 19.7 19.5 19.5 18.3 18.2 18.1 17.4 17.0 15.5 20.4 Table 14. Mean Zn contents (ppm) of mechanically decorticated grains of 16 varieties from the 2007 Short-Variety Trial, averaged over two farmers per village. Village Variety Djelefi Tieble Boulen Tieba Oki Tamia Lebo Tiandougou Coura Marakanio Grinkan Kouladji Koule Gagna Tiguila Drasa Wacoro 13 13 13 14 12 11 11 Seribilia 14 14 13 12 12 13 11 Kenié 17 15 16 13 13 13 12 Teneya 15 14 15 14 13 13 13 Kalague 16 17 15 13 13 13 13 Siranikoro 16 16 16 15 13 14 13 Kafla 15 15 16 15 14 13 13 Keniero 17 17 16 16 14 14 14 Mean 15.3 15.2 14.9 14.0 12.9 12.8 12.3 10 10 10 10 11 9 9 10 12 10 10 11 10 10 10 10 12 11 11 11 11 11 12 11 11 12 13 11 11 12 11 11 13 13 11 11 12 11 11 12 12 12 12 12 11 11 11 11 11 13 11 12 11 12 12 12 14 13 12 12 12 12 11 12 11.9 11.8 11.2 11.2 11.2 11.1 11.0 11.0 18 Kakou Mean 9 10.8 9 11.4 9 12.3 11 12.6 10 12.7 10 12.7 11 12.9 11 13.6 10.1 12.4 Table 15.. Mean Fe, Zn and mechanical decortication yields of varieties in the 2007 “Short” and “Tall” Variety Trials, averaged over eight villages. Short varieties Tall Varieties Variety Palo Kassoroka Yalama Fe Origin* (ppm) G_Pop 13.4 G_LR 13.2 G_Pop 13.0 Zn (ppm) 8.7 8.9 8.8 Dect yld (%) 73 69 70 64 68 50 60 58 Tieble Bamouka Yamasa Sekounioni Bandokablen G_LR G_LR G_Hyb G_LR G_LR 13.0 12.6 12.1 11.7 11.4 8.1 8.1 8.6 8.1 7.2 73 68 70 69 67 5.4 57 Yoka G_Pop 11.2 6.6 69 9.1 5.5 52 Niakafa 11.0 7.2 72 9.0 4.6 55 Yebagasago G_Hyb Interracial 10.7 7.5 66 8.9 4.5 43 Nionifing G_LR 9.5 6.4 61 8.6 4.6 56 Koroba G_LR 9.4 5.7 56 8.4 5.2 41 Bibagalawili 9.4 6.2 68 8.1 4.9 51 Sobani G_LR Interracial Interracial 8.5 6.2 66 Grinkan 8.0 4.7 51 Dougouba 6.2 4.1 Mean 10.0 5.8 56 Mean 11.0 7.3 * G_Pop, G_LR, G_HYb, Inter-racial denote Guinea Poplulation derivative, Guinea Landrace, Guinea Hybrid, and Guinea-Caudatum interracial derivative. 50 67 Variety Tamia Tieble Oki Local Check Djelefi Gagna Boulen Marakanio Tiandougou Coura Kouladji Lebo Kakou Koule Tiguila Drasa Fe Origin* (ppm) G_Pop 13.0 G_LR 12.5 G_Pop 12.0 Zn (ppm) 7.2 7.4 7.0 Dect yld (%) 62 72 59 G_LR G_Pop G_Pop G_Pop G_Pop Interracial Interracial Interracial Interracial Interracial Interracial Interracial Interracial 11.9 11.4 10.2 10.1 9.8 7.5 6.5 5.9 5.5 6.0 9.3 19 4.6 Training of trainers on transformation techniques that assure bioavailability of iron and zinc in meals Training sessions were organized by the IER team on the process of malting, the production of enriched flour, soya milk, and soya soumbala. In addition to 25 women trained in the 3 project areas, a total of 62 women at Bancoumana, 50 at Kéniéro, 46 at Diaratoula, 50 at Alamaniana, 43 at Banco and 44 women at Séribila were trained by the trainers. 4.6.1 Millet malting technique The Malting process consists of using sprouting millet so amylase could be activated, following these steps in the malting process: Picture 1: millet grain washing Picture 3: Millet sprouts Picture 2 : millet spinning Picture 4: sprouted millet 20 Sprouted millet grains are dried in the sun, cleaned to remove roots and leaves. The flour obtained after processing this product is called malt. It is used in small quantities, 5% in a porridge thereby increasing the energy content of the porridge. 4.6.2 Enriched flours These are obtained by combining millet flour (75%), bean flour (25%) and malt flour (5%) The three flours are produced separately before been being mixed according to the prescribed proportions. 4.6.3 Soya soumbala production Soya soumbala production follows several steps. It starts with dry cleaning the soya seeds and dry cooking with continuous steering till they turn brown. This is one of the key steps in soya soumbala production. The second step is to cook the clean seeds with local potassium. The cooked seeds are left to ferment for 3 nights. The product is pounded and formed into bullets. These bullets are used by the women to enrich their meals but are also sold by the women and thus help to increase their income. See pictures 7 and 8. Picture 7 :Before fermentation Picture 8 : After fermentation 21 4.6.4 Soya milk preparation Soya milk is obtained after boiling clean soya seeds in water. The boiled seed is pounded and added with water in the proportion of 1 measure for 3 measures of water. The product obtain is filter and boilt during 10 minutes, after cooling the milk can be drank by children more than 7 months and adults. Picture 9: Boiling soya seeds Picture 11: Filtration Picture 10: Dough of Soya Picture 12: Drinking soya milk 4.7 Training manual development The training manuals on the production of enriched flour, soya milk and soya soumbala and on millet malting was developed. Every manual is organized in topics. Key training steps are recorded with pictures that will be shown to trainees 4.8 Nutrition improved meals by adding Moringa Oleifera, Okra and Baobab leaves. 4.8.1 Moringa Oleifera The Moringa tree was introduced in the project and the porridge will be introduced during the extension phase of the project. The enriched porridges were developed in the Food 22 technology laboratory. They are obtained by mixing decorticated Sorghum powder with moringa leaf powder (5 to 10%) baobab fruit powder (5 to 15%) and okra seed (5 ,10 or 15%). Picture 13. Porridge with Moringa and Baobab leaves The tested variables were : the taste, the color and acceptance: • 90% sorgho+10% of moringa powder • 95 % sorgho +5% of moringa powder • 95% (farine mil+niébé) + 5 % malt • 90% sorgho + 10 % baobab fruit powder • 75% sorgho+10% baobab fruit powder + 15 % soya • 75% sorgho+15% baobab fruit powder +10 put nut • 90 % sorgho +5 % baobab fruit Powder + 5 % moringa powder • 95 % sorgho+ 5 % okra seed powder • 90 % sorgho + 10 % okra seed Powder • 85 % sorgho+ 15 % okra seed Powder Drink based Moringa, okra seed powder and baobab fruit powder: After several tests of drink the formulae, it was found that most of the developed powders had a low lipid content. In general they could be kept for a while without fermentation. However it will be necessary to add lipids to increase the energy value. 23 Table 16 : Powder composition table Humidity Flours (%) 90%sorghum+10% moringa powder 10,25 Proteins (%) Lipids (%) Ashes (%) Energy Kcal/100ml 9,81 4,12 1,67 3878,01 95% sorgho +5% moringa powder 10,10 8 3,22 1,11 3845,05 FC + 5 % malt 9,50 10,06 3,22 0,22 3800,60 90% sorgho + 10 % baobab fruit 10,05 7,87 2,50 0,78 4707,64 75% sorgho+10% baobab fruit+ 15 % soya 9,05 16,25 8,98 0,99 4109,10 75% sorgho+15% baobab fruit +10 put nut 9,35 8,06 8,82 0,88 3992,47 90 % sorgho +5 % baobab fruit + 5 % moringa powder 95 % sorgho+ 5 % okra seed powder 9,85 8,31 3,83 0,89 3045,04 10,10 6,75 2,90 1, 11 3779,20 90 % sorgho + 10 % okra seed powder 9,75 8,06 4,01 1,55 3817,50 85 % sorgho+ 15 % farine okra seed powder 9,69 10,06 4,01 4,89 3881,30 7,45 27,25 8,75 8,43 4389,38 11 19 2,64 8,09 3160 9,50 6,75 3,65 0,88 3728,87 moringa powder Poudre graines de gombo Farine de sorghum 24 4.8.2 Okra Okra seed coffee tastes very good and can be drank with or without milk. The test results were as follows: Tableau 17 : Evaluation of taste of Okra coffee with and without common powder milk. Paramètres d’évaluation Goût Café sans lait de graines de gombo Très Bien Passable Mauvais Bien (%) (%) (%) (%) 13 53 34 Couleur 20 40 40 Odeur 20 53 20 7 Café au lait de graines de gombo Très Bien Passable Bien (%) (%) (%) 20 60 20 40 47 13 40 40 20 4.9 Training of trainers to conduct group discussion A group of 6 women were identified from each of the 6 villages. They were mainly chosen among women association members by their peers. The women were trained as trainers in how to improve Nutrition, based on the outcomes of previous Triple A sessions, the Food Consumption surveys, and other research outcomes. The approach of the Essentials Nutrition Actions was used including how to conduct BCC sessions. In a second training they learned how to use the Picture Aids for the training of mothers in Improved Nutrition. A total of 300 women participated in the disseminaton of training sessions organized in the 6 villages of Bancoumana, Kéniéro, Banco Diarratoula and Allamaniana . The dissemination sessions were organized by 36 trainers. HKI had the main responsibility for this activity. 4.10 The production and broadcasting of radio programs on local radio the 3 sites HKI developed and produced messages to promote Improved Nutrition for broadcasting on local radios. One radio broadcaster was trained per radio station to increase the broadcasters’ understanding of messages in order to better explain messages in group discussions. 25 4.11 The Development of M&E guideline for field agents Guidelines were developed for field agents who conducted group discussion training in the communities. Also, M&E guidelines were developed that were used to monitor mother group activities. 4.12 The production of educational materials for field agents Picture Book Aids were developed and distributed to mothers’ group trainers as well as to community health volunteers living in the An Be Jigi project .It covers all the Essential Nutrition Actions. The picture books aids were developed in close collaboration with the Ministry of Health (Nutrition Division), the National Center for Information and Education for Health (CNIECS), UNICEF, and tested in the project area. This picture book has been introduced to a wider area where HKI has Nutrition Activities, like in the whole region of Koulikoro, two districts in Kayes, one district in Mopti and one in Gao. The advantage of the picture book is that new or local adapted messages and pictures can be added. 4.13 Theater as a communication and education tool HKI team worked again with a Comedian group for the development of themes related to Essential Nutrition Action and other health and nutrition practices. The theater was produced in each of the six villages and regrouped community leaders, elder persons, health center staff, men, women and children. Following the theater, the comedian supported by the health staff questioned the public about the main messages and gave further explanations. The forum theater allowed to inform more people in the target group and to raise further the awareness of the decision makers at the community and household level on the advantages of health and nutrition practices. 4.14 Participation in CoP The 3 An Be Jigi team members participated in the CoP organized in Ouagadougou, where project activities that had taken place since the last CoP, were presented, The five-day workshop was useful for the all participants in terms of information sharing. 4.15 The organization of a final nutrition survey (using KAP method) A Knowledge, Attitudes and Practices (KAP) survey is ongoing and the report will be available by the end of April. The Terms of Reference have been developed by AnBeJigi partners. 26 5. TEAM REPORT SECTION 5.1 Integrated activities During the reporting period the three consortium partners met regularly to discuss the planning of the activities and to organize missions to the field together. Due to time and calendar constraints, joint missions or supervisions were not always feasible. HKI assisted in the supervision of some of IER’s training activities with women groups in the target communities. IER and ICRISAT assisted in the theatre activities in the communities. Findings from the activities of all partners were taken into account as in the development of the BCC messages for target communities. In addition, all consortium partners provided input for development of the training manuals. The consortium has established and adjusted the impact pathway that gives an opportunity to each NGO to determine the key actions to be accomplished, the factors that may affect their accomplishment and to identify the specific research questions. Cultural practices; local nutrition knowledge Approches: Pathway to improved Nutrition Status Voies pour un état Nutritionnel amelioré Sorghum & millet yield Quantity consumed (volume) Ash analysis Methodology to improve selection of Fe/Zn varieties Improved sorghum & millet varieties (Fe/Zn & IP6) Fe, Zn & IP6 analysis Improved sorghum & millet varieties (higher decortication yield) Increased Fe/Zn content in grain Higher decortication yield Improved nutritional status ? Fermentation Knowledge Acceptance, time, motivation Which one is the best? Vit C in porridge Reduced molar ratios (Fe, Zn, IP6) Nutrition education Development of recipes (baobab leaves, okra) Quality of food (Fe & Zn content) Malting Use of new recipes (baobab leaves, okra) Quality of food (Bioavailability) Increased consumption of Fe/Zn rich products Tea consumption Vit C rich products 27 5.2 Insights and lessons learned Identifying the few, most important, research questions to address is of major importance for An BeJigi and the West African CoP for effectively contributing to improved nutrition in the sorghum and millet consuming community. Discussion of team members on this is ongoing, and was further advanced with at the CoP Ouagadougu meeting. The communication materials to be developed, practices and guidelines to be identified and documented for scaling up and out should be guided by these. 5.3. Conclusion and the way forward The ABJ project has achieved some significant results during its last year of implementation. Trainees really appreciate the joint activities which were conducted in their communities. Their increased knowledge of the essential nutrition actions improved their nutrition and hygiene practices, and helped them to better feed their children. At all community levels, trainers continue project activities, BCC sessions, and interpersonal communication. Enriched flour production, soya milk and soya soumbala production provide improved nutrition and at the same time incomes for the women groups in Bancoumanan and women in Seribila Women who planted the Moringa tree are in the hurry to see these trees growth so they could improve their household nutritional status. In order to be able to build these achievements and to continue research for improved nutrition (see 5.2), a concept note has been developed and submitted to the McKnight foundation, for a follow-up phase of the project. The general goal of the follow-up project is to help to improve the health status of children under five and women of child bearing age through biofortified millet and sorghum varieties and improved food processing methods to more effectively deliver micronutrients through staple cereals and other locally available complementary sources. Specific objectives are to (a) quantify nutritional benefits, acceptability, and “food yield” of biofortified staple cereal sorghum and millet varieties; (b) determine the nutritional benefits and acceptability of complementary foods as micronutrient sources; (c) characterize uptake pathways for improved varieties and test approaches for commercialization and scaliing-up biofortified food products in contrasting social and agro-ecological conditions; and (d) improve Behavior Change Communication (BCC) in target communities to ensure that research findings and results are correctly communicated to target communities through the use of tools such image boxes, culinary demonstrations, theater and focus group discussion, etc. 28 6. FINANCIAL REPORT SECTION The financial reports of HKI, ICRISAT and IER will be submitted with final report. 7. WORKPLAN FOR MARCH AND APRIL 2010 (No-cost extension) The most important activity will be the end line Knowledge Attitude Practices (Nutrition) survey. The Terms of Reference are developed and the start of the survey is planned for the second week of March and be able to have the final report by mid-April. It will be conducted by an external consultant. In addition we are working on renewing the contract with local radio stations in the project area and we are planning to offer them contract to cover a period of 6 months. The finalizing (review and printing) of the training modules need to be done. The monitoring of the activities will continue, including the meetings with the consortium members. 29 ANNEX 1 Four-year workplan GENERAL OBJECTIVE (GOAL) SPECIFIC OBJECTIVES 1. Understand general nutritional patterns and food habits, for children under 5 years and mothers, in key agricultural systems OUTPUTS 1.1 Comprehensive report On Sorghum/Pearl Millet Nutrition available based on existing literature and key directions for survey identified 1.2 Nutrition Survey In Test Sites Implemented and Survey Results Analyzed ENHANCED NUTRITION THROUGH SUSTAINABLE BIO-FORTIFICATION OF SORGHUM USING PARTICPATORY VARIETY DEVELOPMENT ACTIVITIES INDICATORS RESPONSIBLE ORGANIZATION AND INDIVIDUAL IER (Salimata Sidibe) Existing document 1.1.1 Participatory analyze reviewed and updated HKI/ICRISAT existing documentation with regard to (mal) nutrition in Reference document elaborated Mali in general and the project sites specifically, # of participants micronutrient content of millet and sorghum and write Workshop technical document available a comprehensive reference document on millet/sorghum Hard or e-copies available nutrition (key number of nutrition/agric workers will of existing information conduct 2-day working session to validate document) (will be combined with start-up workshop) 1.2.1 Implement nutrition 1 baseline survey in 3 of HKI survey (during hungry the 4 sites conducted IER/ AAA/ULPC/AOPP/MRTC season) (this includes: KAP, W/H IN SYNERGY WITH ALIVE nutritional consumption, H/A PROJECT biological data collection) W/A BMI Hb level Serrum ferritin Infection by helminths and AND MILLET CALENDAR YEAR 2 YEAR 1 ANNEX 1 Four-year workplan GENERAL OBJECTIVE (GOAL) SPECIFIC OBJECTIVES 2. Improve understanding of determinants contributing to iron/zinc malnutrition and bioavailability at the household level OUTPUTS 2.1 Key determinants for Fe/Zn bioavailabilty identified and their consequences on target groups assessed 2.2 Improved knowledge of communities on importance of Fe/Zn ENHANCED NUTRITION THROUGH SUSTAINABLE BIO-FORTIFICATION OF SORGHUM USING PARTICPATORY VARIETY DEVELOPMENT ACTIVITIES INDICATORS RESPONSIBLE ORGANIZATION AND INDIVIDUAL Plasmodium infection prevalence Food Consumption data Food processing practices Access and use of health services Hygiene practices (hand washing, access to safe water) 1.2.1 Conduct 24 hour in# 24 hour observation HKI (Marjon Tuinsma) home observation during conducted in all 3 sites ICRISAT (Fred Rattunde) harvest and hungry period 1.2.2 Implement endline Endline survey conducted IER survey, analyze results and in 3 sites HKI/ AAA/ULPC/AOPP/MRTC IN SYNERGY WITH ALIVE produce final document Results analyzed PROJECT Report drafted 2.1.1 Organize 3 community Conceptual HKI (Marjon Tuinsma) based Triple A workshops framework/site available IER/ICRISAT/AAA/ULPC/AOPP (contains presentation of # of participants survey results) 3 workshop documents AND MILLET CALENDAR YEAR 3 YEAR 4 YEAR 1 YEAR 3 YEAR 5 2.2.1 Develop educational materials and organize IEC sessions # of IEC materials produced # of IEC materials distributed HKI (Marjon Tuinsma) AAA/ULPC/AOPP IN SYNERGY WITH ALIVE PROJECT YEAR 3 31 ANNEX 1 Four-year workplan GENERAL OBJECTIVE (GOAL) SPECIFIC OBJECTIVES OUTPUTS nutrition ENHANCED NUTRITION THROUGH SUSTAINABLE BIO-FORTIFICATION OF SORGHUM USING PARTICPATORY VARIETY DEVELOPMENT ACTIVITIES INDICATORS RESPONSIBLE ORGANIZATION AND INDIVIDUAL % of members of focus groups that have heard the message % of members of focus groups that can correctly repeat message % of members of focus groups # of agric workers trained 2.2.2 Ensure comparison of Final evaluation document IER nutrition KAP data at available HKI baseline and endline 2.2.3 Creation of women’s # of women’s groups HKI groups created AND MILLET CALENDAR YEAR 4 YEAR 3 YEAR 4 32 ANNEX 1 Four-year workplan GENERAL OBJECTIVE (GOAL) SPECIFIC OBJECTIVES OUTPUTS 3.1 Base line information on range of decortication losses in farmer preferred varieties as decorticated in villages and soil conditions characterized for participatory trial sites 3.2 Methods/capacity for measuring ease of decortication, decortication yield, ENHANCED NUTRITION THROUGH SUSTAINABLE BIO-FORTIFICATION OF SORGHUM USING PARTICPATORY VARIETY DEVELOPMENT ACTIVITIES INDICATORS RESPONSIBLE ORGANIZATION AND INDIVIDUAL 3.1.1 Grain of diverse Participatory trial results ICRISAT (E. Weltzien) sorghum varieties produced IER-Sor/ULPC/ACOD under farmer-managed conditions in 4 villages in Mandé (AcoD) and 4 villages in Banico (ULPC) AND MILLET 3.1.2 Grain of diverse millet varieties produced under farmer-managed conditions in participatory trials 3.1.3 Observations of decortication traits in participatory breeding trial; sampling whole and decorticated grains in participatory breeding trials; 3.1.4 Physical and chemical lab assessments of whole and decorticated grains 3.1.5 Soil analyses for phosphorus, pH Participatory trial results ICRISAT (E. Weltzien) IER-Cz/AAA AS OF YEAR 1 Statistical analyses, reports IER-LTA (Salimata Sidibe) with ICRISAT, IER-Sor, AAA YEAR 1 Continuing 3.2.1 Refine/Develop field and lab methods for assessing ease-, yield- and embryo loss of decortication CALENDAR YEAR 1, continuing YR1 funding BMZ Yr1 funding BMZ reports IER-LTA YEAR 1 Continuing phosphorus in soil ICRISAT (F. Rattunde) Reports on methods, observation data, consultancy report IER-LTA (Salimata Sidibe) ICRISAT/IER-Sor/IER-Cz/AAA AS OF YEAR 1 Funded through ALIVE YEAR 1 33 ANNEX 1 Four-year workplan GENERAL OBJECTIVE (GOAL) SPECIFIC OBJECTIVES OUTPUTS loss of embryo 4. Characterize and understand general impact of decortication on nutrient losses / availability 4.1 Decortication losses (weight, ash, Fe, Zn, Phytate) using different methods characterized (at point of acceptable grain quality, over range encountered at household level) 4.2 More easily measures of nutrient retention/loss identified 4.3 Fe, Zn, Phytate ENHANCED NUTRITION THROUGH SUSTAINABLE BIO-FORTIFICATION OF SORGHUM AND MILLET USING PARTICPATORY VARIETY DEVELOPMENT ACTIVITIES INDICATORS RESPONSIBLE CALENDAR ORGANIZATION AND INDIVIDUAL (IER-LTA); sampling/observing sorghum grain (IER-Sor/ICRISAT) and millet grain (AAA/IERCz/ICRISAT); Food Science consultant advise on grain die techniques (ICRISAT) 4.1.1 Decortication at Reports on results of IER-LTA (Salimata Sidibe) YEAR 1 different levels via Manual, parameters tested TADD and Mechanical methods of sorghum, millet, maize 4.2.1 Establish grain sampling protocol (IERLTA/ICRISAT) that avoids contamination, preliminary samples analysed for soil contaminants (Al,Ti,Cr) (ICRISAT-Waite Univ.), protocol revised based on results 4.3.1 Analyses for Fe, Zn, Protocol IER-LTA (Salimata Sidibe) ICRISAT YEAR 1 Harvest plus funding Fe, Zn Phytate values; Year 1-2, ICRISAT (lab to AS OF YEAR 1 34 ANNEX 1 Four-year workplan GENERAL OBJECTIVE (GOAL) SPECIFIC OBJECTIVES 5. Elaborate <<nutrition friendly>> participatory variety selection methodology OUTPUTS contents of whole-, decorticated-grain and bran, sources of variation (G, E, GxE), estimates of predicted gains from alternative selection criteria 4.4 Strengthened capacity of IER for micronutrient analyses 5.1 Selection protocol for participatory variety improvement programs; first superior varieties identified ENHANCED NUTRITION THROUGH SUSTAINABLE BIO-FORTIFICATION OF SORGHUM USING PARTICPATORY VARIETY DEVELOPMENT ACTIVITIES INDICATORS RESPONSIBLE ORGANIZATION AND INDIVIDUAL Phytate of wholegenetic correlations identify) (F. Rattunde) decorticated-grain, bran of 5 varieties contrasting for Year 3-4, IER-LTA decortication yield from 4 villages per zone; genetic correlation of direct with indirect measures of Fe and Zn contents (lab to confirm) 4.4.1 Training IER-LTA staff Training report IER-LTA (Salimata Sidibe) in techniques for iron and zinc analysis at ITA, Senegal AND MILLET 5.1.1 a) On-Farm varietal trials of sorghum and millet; b) Evaluation of larger number of varieties for key physical grain traits previously identified; c) analysis micronutrient/Phytate contents; d) Bio-available Fe and Zn levels estimated based on Fe/Zn and Phytate values; e) Multi-variate analyses of contribution of physical (indirect) grain observations to bio-available AS OF YEAR 2 reiterative process On-farm variety trials; number of varieties analysed, direct and indirect measures of MN value, selection protocol. ULPC (M. Diarra) AAA (S. Cissoko) ACOD (A. Sangaré) IER-Sor, IER-Cz, IER-LTA (D. Dramé) ICRISAT, HKI CALENDAR Harvest plus funding for Yr 1 YEAR 1 35 ANNEX 1 Four-year workplan GENERAL OBJECTIVE (GOAL) SPECIFIC OBJECTIVES 6. Introduce changes in cooking methods, meal preparation, use of specific foods, behaviours for improving micronutrient nutrition in children and mothers OUTPUTS 6.1 Processing/cooking/ feeding method improvements identified, implemented, monitored and accepted ENHANCED NUTRITION THROUGH SUSTAINABLE BIO-FORTIFICATION OF SORGHUM USING PARTICPATORY VARIETY DEVELOPMENT ACTIVITIES INDICATORS RESPONSIBLE ORGANIZATION AND INDIVIDUAL Fe/Zn contents; f) Draft selection protocol for micronutrient enhancement based on quantitative genetic analyses; g) feedback sessions with participating farmers 6.1.1Based on outcome 2.1, 3 key determinants/site AAA (S. Cissoko) reinforce/introduce use of identified ULPC (M. Diarra) specific foods, practice and ACOD (A. Sangaré behavioural changes by IER (D. Dramé) season for improving HKI/ICRISAT micronutrient nutrition in U5 children and mothers AND MILLET CALENDAR AS OF YEAR 2 reiterative process 36 ANNEX 1 Four-year workplan GENERAL OBJECTIVE (GOAL) SPECIFIC OBJECTIVES 7. Institutionalize participatory sorghum and millet selection for nutritional value OUTPUTS 7.1 Documentation of selection protocol and guidelines; NARS capacities enhanced; 7.2 Documentation of key interventions and “Packages of practices” for synergy in micronutrient enhancement ENHANCED NUTRITION THROUGH SUSTAINABLE BIO-FORTIFICATION OF SORGHUM USING PARTICPATORY VARIETY DEVELOPMENT ACTIVITIES INDICATORS RESPONSIBLE ORGANIZATION AND INDIVIDUAL 6.1.2 Regularly monitor # of visits to participating AAA (S. Cissoko) implementation of improved communities ULPC (M. Diarra) action (this is a reiterative Mission reports ACOD (A. Sangaré) process) IER (Salimata Sidibé) HKI/ICRISAT 6.1.3 Verify acceptance of # of households still AAA (S. Cissoko) improvements by the practising improved ULPC (M. Diarra) participating communities behaviour one year after ACOD (A. Sangaré) end of project IER (Salimata Sidibe) HKI/ICRISAT 7.1.1 a) On-Farm varietal On-farm trials, guidelines ULPC (M. Diarra) selection for MN value, b) ACOD (A. Sangaré) draft and validated IER-Sor, IER-LTA, IER-Cz guidelines for selection (Salimata Sidibe) programs, c) link with “Seed AAA (S. Cissoko) Systems” for seed and ICRISAT, HKI information dissemination 7.2.1 a) develop/test ways of #communications HKI (Marjon Tuinsma) combining superior varieties (presentations, bulletins, & processing techniques, b) articles, websites), # develop and disseminate workshop participants mass media presentations on importance/ways of improving MN nutrition c) NARS / Development Organization training AND MILLET CALENDAR AS OF YEAR 2 on a monthly basis YEAR 5 AS OF YEAR 3 YEAR 4 37 ANNEX 2 Succes stories or most significant change. • The village of Seribila. A newborn stays 72 hours after birth without breastfeding because tgehe mother contracted mastitis, The village found alternative solutions to feed the newborn when HKI proposed that baby be breastfed by another mother. It was s not a rule in their community for a baby to be breast fed by another mother. HKI undertook to explain how important it was to breast feed this baby instead of giving him cow milk or milk powder. After the meeting with the Head of the household and , advocacy addressed to the chef of village, it was accepted that another mother in the same household breastfeed the baby till to his mother was cured. Because we were able to change an old behavior which was in this community according of which it was forbidden for a woman to breastfeed a baby if it was not her won, we saw this as a significant change. • The second significant change was noticed in Banko village:pen? During the dissemination of the trainers’ training session, a grandmother asks this question: How should I feed??my daughter’s baby since she is pregnant, with a baby who is only 6 months old? A trainer answered her and showed an example of a child whose mother got pregnant 6 months after giving birth and whose baby was very healthy and well nourished: The trainer advised the grandmother to tell her daughter to continue to breastfed her baby until she was 8 months pregnant but she should add complementary feeding after 6 months, Sshe advised pregnant womennot do stop breast feeding because of pregnancy. The grandmother argued that breastfeeding is forbidden for a pregnant woman because the milk becomes warm and baby would fall sick. The trainer explained that it not the mother milk which is responsible for diarrhea but the food wwas given to the baby after breastfeeding was stopped. This is a big change in the behaviors of this community. • The third significant change we noticed after project interventions (training of trainers, BCC sessions, and theater forum sessions), Community health agents noticed the increasing number of pregnant women seeking ante natal care in their village. Also in Seribila villages , women trainers decided to conduct BCC session at the CSCom level, this also increase the number ante natal care seekers. • Enriched Flour : In Keniero village a mother who had no milk to feed her 6 monts old baby sought help. because of the baby’s constant crying, , she was not able to work as the other mothers, after participating in the training on enriched flour preparation for children, she undertook to prepare and feed her baby with porridge made from enriched flour. Since this time her baby has been healty, happy and well nourished.,. making it possible for her to work. • One woman cooked the enriched flour for her sick husband, After he ate the porridge the man started eating and regained his health, To continue the joint work of soya milk and soya soumbala, womens group member’s decided to plant soya in a field, One woman reported that since the date her husband ate sauce cooked with soya soumbala he had decided to stop to eating any sauce cooked in his household without soya soumbala. The most significant change is that the women group members are now organized to prepare and sell enriched flour, soya soumbala which will also provide them with money. 39 Annex 3. Training materials Transformation MINISTERE DE L’AGRICULTURE MALI =-=-=-=-= BUT - UNE FOI INSTITUT D'ECONOMIE RURALE =-=-=-=-= CENTRE REGIONAL DE RECHERCHE AGRONOMIQUE DE SOTUBA =-=-=-=-= LABORATOIRE DE TECHNOLOGIE ALIMENTAIRE REPUBLIQUE DU UN PEUPLE - UN TRANSFORMATION DES CEREALES ET LEGUMINEUSES ALIMENTAIRES (dans les zones de Mandé et Doïla du 10 au 21 Janvier 2008) COULIBALY Salimata SIDIBE DIALLO Fatimata CISSE KONE Mariam DEMBELE TOURE Fadimata MAÏGA Janvier 2008 40 Jour 1 9 h-9h 30 10 h-11h 11 h- 13 h Jour 2 Jour3 Jour 4 8h 30- 9 h Arrivée, Installation, dans les 2 sites présentation des participants et des fiches techniques et identification des attentes Notions d’hygiène Trempage du sorgho ou du mil pour maltage pendant 7 heures Préparation du soumbala et farine de soja - Triage, torréfaction du soja, lavage - cuisson d’une partie du soja dépelliculé pour soumbala - séchage de l’autre partie pour farine infantile - Mise en fermentation des grains de soja cuits - Décorticage du niébé et séchage TP maltage LTA, ICRISAT, HKI ACOD/ULPC LTA, ICRISAT, HKI ACOD/ULPC LTA, ICRISAT, HKI ACOD/ULPC LTA, ICRISAT, HKI ACOD/ULPC LTA, ICRISAT, HKI ACOD/ULPC 9h- 11h Rappel de la journée précédente TP décorticage et ou lavage des céréales (sorgho ou mil ou maïs) TP mouture et séchage des farines de céréale 11h- - Sensibilisation sur la malnutrition, avantage de la bouillie enrichie LTA, ICRISAT, HKI ACOD/ULPC 8 h 30-9h TP maltage LTA, ICRISAT, HKI ACOD/ULPC 9h 11h LTA, ICRISAT, HKI ACOD/ULPC 8h30-9h Rappel de la journée précédente Triage et torréfaction de l’arachide pour farine infantile Séchage (suite) TP maltage 9h-9h30 TP lait de soja LTA, ICRISAT, HKI ACOD/ULPC 9h30-13 h TP lait de soja LTA, ICRISAT, HKI ACOD/ULPC 15h Torréfaction de la farine de sorgho (ou mil) et de celle du niébé LTA, ICRISAT, HKI ACOD/ULPC LTA, ICRISAT, HKI ACOD/ULPC 41 Jour 5 Mélange des farines (3 parts sorgho+1 part niébé) Mélange des farines (3 sorgho+1 soja+1/2 arachide) Séchage du soumbala 8h30- 9h 30 Fin maltage, séchage des grains germés Elimination des pédicelles et racines par léger pilage et vannage - Mouture du grain germé et tamisage de la farine (malt) 9h30-12h Préparation de la bouillie maltée : Mélange des farines (3 mesures de farine de céréale+1mesure de niébé+1/4 mesure de malt). LTA, ICRISAT, HKI ACOD/ULPC 42 HYGIENE ET QUALITE 1. Généralités sur l’hygiène alimentaire 1.1. Définitions de l’Hygiène L’hygiène est l’ensemble de règles et pratiques relatives à la conservation de la santé (Petit Larousse). L’hygiène des aliments représente l’ensemble des conditions et mesures nécessaires à l’assurance de la sécurité et de la salubrité des aliments à toutes les étapes de la chaîne alimentaire. La qualité hygiénique d’un aliment désigne la non toxicité de l’aliment à court et moyen terme. Définition de la qualité La qualité se définit d’abord par rapport à une attente du consommateur mais aussi par rapport à diverses contraintes du producteur et/ou fournisseur. Il en existe plusieurs définitions parmi lesquelles on peut noter celles ci dessous : La qualité est l’ensemble des propriétés et caractéristiques d’un produit ou d’un service qui lui confèrent l’aptitude à satisfaire des besoins exprimés ou implicites (AFNOR,1994). Selon le Dictionnaire Petit Larousse, c’est la manière d’être, bonne ou mauvaise, d’une chose ; état caractéristique (Aptitude à satisfaire le client). Pour les produits alimentaires, on peut scinder la qualité en plusieurs volets : La qualité nutritionnelle : aptitude de l’aliment à bien nourrir le consommateur ; La qualité hygiénique : la non toxicité de l’aliment à court et long terme ; La qualité organoleptique : caractéristiques se rapportant aux sensations gustatives, olfactives, tactiles, visuelles,… La qualité de service : la commodité d’emploi du produit La qualité technologique : c’est l’aptitude d’une matière première à un traitement technologique donné ; La qualité réglementaire : elle correspond aux standards minimums fixé par les règlements ou les normes en vigueur dans le but d’assurer au consommateur une protection minimale quant aux différentes qualités évoquées ci-dessus. 2. Hygiène 2. 1. Pourquoi l’hygiène C’est pour prévenir contre un certain nombre de dangers pouvant avoir de lourdes conséquences aux plans social et économique : dangers biologiques (microbes et autres parasites) dangers physiques (corps étrangers, morceaux de fer, de ver, de bois ….) dangers chimiques (métaux lourds, pesticides, résidus de médicaments, toxines). 2.2. Principes d’hygiène - Réduire ou minimiser les contaminations - Éviter ou ralentir leur augmentation, voire les détruire 43 Les règles de base consistent essentiellement en la mise en application régulière et correcte des mesures suivantes : Veiller rigoureusement et en permanence à la propreté des locaux , des matériels de production et du personnel ; Prendre toujours des mesures préventives aux différentes étapes de la chaîne de production et contrôler leur bonne application. 2.3. Les enjeux de l’hygiène Les conséquences du non- respect de l’hygiène se font sentir avec plus ou moins d’acuité à plusieurs niveaux : Pour les aliments : on peut aboutir à une altération de la qualité marchande (modifications des caractéristiques organoleptiques et physico-chimiques sans dangers pour le consommateur) et/ou de la qualité hygiénique (prolifération microbienne, présence de mycotoxines) rendant l’aliment dangereux pour le consommateur. Pour le consommateur : les intoxications et maladies d’origine alimentaire telles que les salmonelloses, hépatites, diarrhées, …sont nombreuses. Les conséquences peuvent aller d’une simple indisposition passagère à des cas graves entraînant la mort. Les coûts humains et médicaux de ces intoxications et maladies sont souvent très élevés. Pour l’entreprise : les conséquences économiques sont très importantes ; elles commencent souvent par une perte de clientèle pour atteindre chute brutale de l’image de marque pour finir par la faillite avec parfois des poursuites judiciaires pour les responsables de l’entreprise. La maîtrise de l’hygiène de la production à la consommation finale du produit est donc essentielle pour éviter les conséquences négatives sur la santé publique et l’économie. L’hygiène et la sécurité des aliments intéressent de plus en plus les pouvoirs publics et les consommateurs sont désormais plus vigilants et plus exigeants. Chacun pour ce qui le concerne a un rôle à jouer Nettoyage, entretien et hygiène du personnel Des installations et procédures appropriées devraient être mises en place pour assurer que: - toutes les opérations nécessaires de nettoyage et d’entretien soient conduites efficacement; et - un degré approprié d’hygiène corporelle soit maintenu. 44 Le lavage des mains Moments de lavage des mains Avant de commencer le travail Avant et après avoir mangé Après avoir manipulé des objets sales (une poubelle par exemple) Après usage des toilettes Après avoir éternué ou toussé Comment doit on se laver les mains ? Mouiller les mains Prendre le savon Frotter bien les mains Sécher bien les mains Dosage de l’eau de javel Pour le rinçage Avant chaque journée de production procéder à un rinçage du matériel avec de l’eau javellisée en prenant 3 gouttes pour 1 litre d’eau potable. 45 4. Les Bonnes Pratiques de Fabrication Elles reposent sur les principes suivants : Avoir toujours présent à l’esprit que le client est le juge final de la qualité de nos produits et qu’il ne faut prendre aucun risque en matière de qualité et de sécurité sanitaire des denrées alimentaires Bracelets et autres bijoux devraient être retirés avant de rejoindre son poste de travail Contrôler régulièrement la qualité à son poste de travail fait partie des consignes à suivre Démonter si nécessaire le matériel de travail en fin de journée pour le nettoyage Entreposer les produits et les matières premières en prenant soin de les identifier clairement Fumer dans un atelier de fabrication est formellement interdit Gardez-vous des habitudes, un changement du produit est toujours possible Habillez-vous seulement avec des vêtements propres qui sont mis à votre disposition Ignorer les Bonnes Pratiques de Fabrication et d’Hygiène est une faute grave car il ne faut prendre aucun risque Jeter immédiatement tous les produits défectueux dans les contenants placés à cet effet dans les ateliers Lavez vous toujours les mains avant de rejoindre votre poste de travail Manger dans un atelier de fabrication est formellement interdit Nettoyer les équipements et le matériel de production avec les produits et détergents approuvés Oter rapidement toute accumulation de déchets près d’une ligne de production Porter toujours le filet ou casque protecteur mis à votre disposition avant de rejoindre votre poste Questionner sans délai votre chef d’équipe si quelque chose vous parait anormale Tenir son poste de travail propre et ordonné c’est respecter ses compagnons de travail Utiliser seulement des produits qui portent une identification, c’est éviter l’erreur Veiller à ce que rien ne puisse tomber dans le produit ou dans son emballage et vérifier toujours le produit fini avant de l’expédier car le prochain contrôleur c’est le client X’tra c’est ce que nous voulons entendre des clients lorsqu’ils parlent de nos produits, des hommes et femmes qui les ont fabriqués 46 Yaka est l’avenue ouverte à tous les risques d’erreur, n’improvisez pas Zéro défaut est l’objectif que nous devons avoir toujours présent à l’esprit pour satisfaire les clients 47 FICHE THECHNIQUE SUR LA PRODUCTION DE LA FARINE COMPOSEE MALTEE La farine composée maltée est un aliment de complément destiné aux enfants de plus de 6 mois. Elle est à base de mil, de niébé et de malt. Cette production comporte deux principales phases : la production de la farine composée mil+niébé, et le maltage du mil. A celles-ci s’ajoute la préparation de la bouillie. 1. PREPARATION DE LA FARINE COMPOSEE MIL+NIEBE OPERATIONS DESCRIPTION/REMARQUES Nettoyage Débarrasser la matière première (mil et niébé) de toutes les impuretés par un nettoyage mécanique ou manuel. Décortiquer le mil et le niébé à l’aide d’un décortiqueur à disque abrasif ou de type engelberg ou manuellement au mortier et pilon. Décorticage Vannage et lavage Eliminer le son par vannage et le sable par lavage à grande eau. Séchage Torréfaction Sécher au soleil ou à l’aide d’un séchoir le mil et le niébé. Torréfier (griller) les grains de mil et de niébé, à l’aide d’un torréfacteur ou dans une marmite, pour réduire l’humidité et les facteurs anti-nutritionnels. Mélange Mélanger le mil et le niébé respectivement à 75% et 25% du poids final du mélange. Mouture Faire la mouture avec un broyeur à marteau muni de grille de 0.7 mm d’ouverture de maille, puis tamiser avec un tamis d’ouverture ≤ 0.3 mm . Conditionnement Emballer la farine dans des sachets en polypropylène ou polyéthylène épaisse. 48 2. MALTAGE DU MIL Le maltage est un processus de germination contrôlée du mil. Les principales opérations de production du malt de mil sont les suivantes : OPERATIONS DESCRIPTIONS/REMARQUES Nettoyage du mil Nettoyer par vannage et lavage du mil pour éliminer les impuretés (sable, autres). Trempage Tremper les grains de mil jusqu’à submersion totale dans de eau tiède pendant 7 heures. Essorage et mise en germoir Essorer les grains à l’aide d’un tamis pour réduire l’excès de l’eau. Mettre les grains dans un panier en osier ou sur un lit de germination en inox et couvrir. Germination er 1 jour : 2 ème 3 ème 5 ème jour : 4 ème jour : Laver les grains à grande eau le matin, à midi puis le soir sans les frotter, les essorer puis mettre en germoir. e e Du 2 au 4 jour, arroser les grains en germoir sans les inonder matin et soir. e Au 5 jour, interrompre la germination par un séchage solaire ou à l’aide d’un séchoir. Elimination des gemmules Les grains séchés sont légèrement pilés puis vanner pour éliminer les gemmules. Mouture Faire la mouture à l’aide d’un broyeur à marteau Puis tamiser pour éliminer le son. Conditionnement Le malt ainsi obtenu est conditionné en sachets plastiques ou dans un récipient hermétique avant son utilisation dans la préparation de la bouillie. 3. PREPARATION DE LA BOUILLIE Mélanger la farine composée sorgho ou mil+niébé et le malt (farine de mil germé) respectivement à 95% et 5% du poids final de la farine composée maltée ou 1 mesure du mélange mil+niébé et ¼ de mesure de malt ; Délayer 1 mesure de ce mélange dans 3 ou 4 mesures d’eau tiède contenue dans une casserole, remuer et poser sur le feu et laisser cuire pendant 7-10 minutes. Ajouter du jus de citron en fin de cuisson et éventuellement un peu de sel, du sucre ou un peu d’huile ou de beurre pour plus d’énergie. 49 FICHE THECHNIQUE SUR LA PRODUCTION DE LA FARINE COMPOSEE La farine composée est un aliment de complément destinée aux enfants de plus de 6 mois. Elle est à base de céréale (sorgho, maïs ou mil), de soja et d’arachide. OPERATIONS Nettoyage du mil ou du sorgho, du soja et de l’arachide DESCRIPTION/REMARQUES Débarrasser la matière première (mil, soja arachide) de toutes les impuretés par un nettoyage mécanique ou manuel puis les trier Décorticage/ vannage et lavage - Décortiquer la céréale (mil, ou maïs ou sorgho) à l’aide d’un décortiqueur à disque abrasif ou de type engelberg ou manuellement au mortier et pilon. Eliminer le son des céréales par vannage et le sable par lavage à grande eau. - Torréfier soja jusqu’à ce que les pellicules des graines comment à craquer - les verser dans l’eau tiède puis frotter pour éliminer les pellicules - Faire sécher les graines de soja dépelliculées Séchage Sécher au soleil ou à l’aide d’un séchoir le mil ou le maïs et le soja. Dans le cas où la mouture sèche n’est pas possible, procéder à la mouture humide du mil puis sécher la farine. Torréfaction - Torréfier (griller) les grains de mil de maïs ou de sorgho à l’aide d’un torréfacteur ou dans une marmite, pour réduire l’humidité et les facteurs anti-nutritionnels. Dans le cas d’une mouture humide, faire sécher la farine de mil puis la torréfier. - Torréfier l’arachide Mélanger 3 mesures de mil ou de sorgho + 1 mesure de soja + ½ mesure d’arachide. Mélange Mouture Faire la mouture avec un broyeur à marteau muni de grille de 0.7 mm d’ouverture de maille, ou manuellement tamiser avec un tamis d’ouverture ≤ 0.3 mm (nylon). Conditionnement Emballer la farine dans des sachets en polypropylène ou polyéthylène épaisse. FICHE THECHNIQUE SUR LA PRODUCTION DU SOUMBALA DE SOJA OPERATIONS Nettoyage du soja DESCRIPTION/REMARQUES Débarrasser le soja de toutes les impuretés par un triage manuel 50 Torréfaction et lavage - Torréfier soja jusqu’à ce que les pellicules des graines comment à craquer - les verser dans l’eau tiède puis frotter pour éliminer les pellicules Cuisson - Cuire les graines de soja en ajoutant de la potasse locale jusqu’à leur ramollissement (environ 2 à 3 heures) - A la fin de la cuisson, éliminer l’excès d’eau et refroidir légèrement Fermentation - Les graines cuites sont placées dans un sac en polyéthylène puis recouverte pour favoriser la fermentation qui dure 2 à trois nuits selon les saisons. Séchage - Les graines fermentées sont séchées directement ou broyer pour former une pâte qu’on peut modeler comme dans le cas du soumbala de néré. 51 FICHE THECHNIQUE SUR LA PRODUCTION DU LAIT DE SOJA Ingrédients 1 mesure de soja 4 mesures d’eau Sucre et sel selon le goût Préparation OPERATIONS OBSERVATIONS Nettoyage Triage des graines De soja impuretés et les avariées puis laver. Cuisson - Verser les graines dans 6 mesures d’eau bouillante et laisser cuire pendant 20 minutes. Ne pas couvrir. - Laver pour éliminer la pellicule et égoutter les graines cuites Mouture des graines Moudre les graines jusqu’à l’obtention d’une pâte lisse. - Ajouter 4-5 tasses d’eau bouillante puis filtrer le mélange à l’aide d’un tissu blanc et propre. Cuisson du lait - Ajouter du sucre et du sel selon votre goût. - Faire cuire le lait pendant 15 minutes. Ne pas couvrir. Refroidissement et ensachage Conservation Refroidir le lait dans un récipient. REMARQUES ET RELEVE DES DONNEES Eviter la caramélisation des graines Eviter l’humidité - Le lait peut être conservé pendant 12 heures hors du réfrigérateur. - Le résidu peut être séché et utilisé dans d’autres plats (soupes, akara) 52