abstract

EFFECT OF BONE MICROENVIRONMENT IN JAW OSTEOSARCOMA
DEVELOPMENT
Abstract ID : 1158
Submitted by : Françoise Redini the 2016-02-08 17:15:49
Category : Others
Typology : Communication orale / Oral communication
Status : Validated
Authorisation to disclose : Yes/Oui
------------------------------------Background: Osteosarcoma is the most frequent malignant bone tumor in children and adolescents. Jaw osteosarcoma
(JOs) differs from the long bones osteosarcoma (LBOs) in an onset two decades later and a lower metastatic potential
with better survival. The treatment of the JOs remains complicated because of the aesthetic and functional disabilities
due to the surgery. The aim of our work is to develop for the first time a preclinical model of JOs in order (i) to investigate
the potential role played by the bone microenvironment in the JOs development, and (ii) to understand a different clinical
behavior as compared with LBOs.
Materials and methods: Using syngenic and xenogenic models from mouse and human osteosarcoma cell lines, and a
tumor xenograft induced by patient derived JOs biopsy, we developed jaw osteosarcoma models in mice. The jaw model
was compared to the tibia induced osteosarcoma model and characterized according to clinical parameters (tumor
growth, metastasis dissemination), microCT imaging and histologic settings. A comparative analysis of bone markers at
transcriptomic and immunohistochemical levels was carried out between the two locations. The protein expression in
bone microenvironment was comparatively studied in human biopsies from jaw and long bones osteosarcomas by using
tissue micro-array (TMA). Zoledronic acid targeted therapy was realized in jaw osteosarcoma model looking for a
possible effect on the tumor growth and the metastatic development.
Results: The tumor growth was higher in the tibia induced osteosarcoma site than in the jaw site mostly in the xenogenic
model. Lung metastases were found in both models without correlation with the tumor volume. The morphometric
characterization by microCT showed mixed osteogenic and osteolytic lesions as described in human disease and no
histologic difference was found regardless of the model studied. Immunohistochemical, transcriptomic and TMA
comparative studies are still in progress. Zoledronic acid was shown to be an effective therapy to reduce the tumor
growth in mice jaw osteosarcoma.
Conclusion: We describe the first animal model of jaw osteosarcoma in non-GM mice and its potential use in specific
therapeutic targeting.
------------------------------------Keywords : osteosarcoma, microenvironment, bone resorption, angiogenesis
Authors :
References : , , ,
Authors
Françoise REDINI 1, Hélios BERTIN 1, Romain GUIHO 1, Malika GANTIER 1, Jérôme AMIAUD 4, Julie LONGIS 5,
Benoit PIOT 5, Pierre CORRE 5, Anne BROUCHET-GOMEZ 6, Dominique HEYMANN 1,
1. faculté de médecine, INSERM UMR957, Nantes, FRANCE
2. Faculté de Médecine, INSERM UMR957, Nantes, FRANCE
3. Faculté de médecine, INSERM UMR957, Nantes, FRANCE
4. Faulté de médecine, INSERM UMR957, Nantes, FRANCE
5. stomatology, CHU Hôtel Dieu, Nantes, FRANCE
6. Pathology-CRB, Oncopôle, Toulouse, FRANCE
Authors (raw format)
REDINI Françoise - email : [email protected] Institution : INSERM UMR957 Department : faculté de
médecine City : Nantes Country : FRANCE Speaker : Yes
BERTIN Hélios - email : [email protected] Institution : INSERM UMR957 Department : Faculté de Médecine City :
Nantes Country : FRANCE Speaker : No
GUIHO Romain - email : [email protected] Institution : INSERM UMR957 Department : Faculté de médecine
City : Nantes Country : FRANCE Speaker : No
GANTIER Malika - email : [email protected] Institution : INSERM UMR957 Department : Faculté de
médecine City : Nantes Country : FRANCE Speaker : No
AMIAUD Jérôme - email : [email protected] Institution : INSERM UMR957 Department : Faulté de
médecine City : Nantes Country : FRANCE Speaker : No
LONGIS Julie - email : [email protected] Institution : CHU Hôtel Dieu Department : stomatology City : Nantes
Country : FRANCE Speaker : No
PIOT Benoit - email : [email protected] Institution : CHU Hôtel Dieu Department : stomatology City : Nantes
Country : FRANCE Speaker : No
CORRE Pierre - email : [email protected] Institution : CHU Hôtel Dieu Department : stomatology City : Nantes
Country : FRANCE Speaker : No
BROUCHET-GOMEZ Anne - email : [email protected] Institution : Oncopôle Department : Pathology-CRB
City : Toulouse Country : FRANCE Speaker : No
HEYMANN Dominique - email : [email protected] Institution : INSERM UMR957 Department :
Faculté de médecine City : Nantes Country : FRANCE Speaker : No