Nucleic Acid Isolation System

Nucleic Acid Isolation System
For Genetic Analyses Of Blood Samples
Smart Purification Tool for Genetic
The QuickGene-610L can perform DNA isolation of 6 whole bl
High throughput in routine analytical work on demand.
QuickGene-610L Nucleic Acid Isolation System is semi-automated, optimized for genetic
STEP 1: Large-scale Analysis
1 Process valuable samples simultaneously
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Sample
STEP 2: Quick Isolation
2 6 samples within 12 min
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Isolation
2ml*
×
12min
6 samples
* In case of whole blood
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QuickGene - 610L
Revolutionary Porous Membrane
Scale up for Genetic Research Studies
2ml of sample
Revolutionary Porous Membrane
Proprietary 80 m thick porous
membrane of the cartridge
effectively absorbs
Scale up
×10
nucleic acids in hydrophobic
solvents, separating from
80 m
proteins and lipids.
The integration achieves
stable and reproducible
membrane separation-
A novel cartridge of QuickGene-610L enables the processing of a
large volume of samples, ten times (×10) more than QuickGene810 cartridges, for nucleic acid isolation.
isolation performance.
Analysis Research
lood samples (2ml each), within 12 min.
For research use only
c analyses of blood samples.
STEP 3: Quality Evaluation
STEP 4: No Separate Purification Process
3 Mild isolation provides DNAs of higher quality,
4 The DNA samples are suitable for downstream
fewer cleavage.
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Analysis
Results
Sequencing
Genotyping
PCR
SNP Validation
DNA Sequencer
Genetic Analysis
Real Time PCR
Linkage Analysis
DNA Microarrays
Cancer Research
Fewer-cleaved, High-purity
50ѥ g*
Genomic DNA 50
*Yeild may vary according
to samples.
Genetic Disease
Longer DNA Samples
Q
S
DNA isolated by QuickGene has
1.0
remarkable lengths of 97 kbp
through its gentle separation
0.8
process under low pressure
0.6
conditions.
97 kbp
OD
M
High -purity
Traditional spin-column isolation
0.4
method with a centrifuge has been
0.2
HLA Typing
QuickGene-610L
known to yield shorter DNA
24.5 kbp
fragments than QuickGene due to
the cleavage of DNA molecules.
0.0
220
260 300 340 380
WaveLength (nm)
420 A spectrum of DNA sample
purified by QuickGene-610L
DNA samples obtained by QuickGene-610L is of such high
quality that the purification process is not required to remove
M: Midrange PFG maker II
Q: QuickGene-610L
S: Spin Column
proteins and chaotropic salts and can be directly submitted to
analysis such as PCR, etc.
High purity and high yield without centrifugation
Three pressurizing stages-binding, ■ Isolation of DNA from whole blood
washing and elution -occur automatically
P re t re a t m e n t
in QuickGene-610L
in the unit. Because of the outstanding
Pressurizing process ( no need for centrifugation )
adsorptive and desorptive properties
Addition of
reagent
of the membrane, high-purity nucleic acid
Binding
Washing
can be obtained easily at low pressure
without any complex processes such as
Sample
（2mL）
centrifugation.
＋
Elution
Protease
■ Adsorption of nucleic acids
Owing to their hydrophilic
properties, nucleic acids get
adsorbed onto the membrane,
while proteins and lipids, which
are comparatively hydrophobic,
tend to seep out of the membrane.
Membrane
DNA
Hydrophilic surface
Waste
Waste
RNA
Lipid
Surfactant
High purity DNA
（ca. 50μg）
Protein
Isolation Kits for QuickGene-610L
QuickGene DNA whole blood kit L
QuickGene isolation kit (for 48 samples) contains all reagents and tubes necessary
for DNA isolation from whole blood in a single package.
QuickGene isolation kit
(for 48 samples)
Lysis buffer
QuickGene DNA
whole blood kit L
Reference code DB-L
Pretreatment enzyme
○
Lysis buffer
○
Wash buffer
○
Elution buffer
○
Cartridges
○
Waste tubes
○
DNA Yield
FDƄJZKROHEORRGPO
Pretreatment enzyme
Wash buffer
Elution buffer
Waste tube
Cartridge
Specifications of QuickGene-610L
Overview
Physical specifications
Automated stages: Sample binding, washing, and elution
Throughput: 1 to 6 samples per run
Display: LCD (16 characters x 1 line)
Operating conditions
Supply voltage: 100 ∼ 240V
Power supply frequency: 50/60Hz
Operating conditions: Temperature: 15 - 30℃
Humidity: 30 - 80% (non-condensing)
Power consumption: 100W
448
125
Dimensions: 573(W) × 332(D) × 398 (H)mm
Weight: approx. 24kg
398
Isolation kit
QuickGene DNA whole blood kit L (for 48 samples)
For research use only
332
573
Application Guides Available on our website.
Unit :mm
h t t p : / / w w w. ku rab o . co . j p / b i o / E n g l i s h /
KURABO INDUSTRIES LTD.
Bio-Medical Department
Kurabo Neyagawa Techno Center 3F,
14-5, Shimokida-Cho, Neyagawa, Osaka 572-0823, Japan
TEL :+81-72-820-3079 FAX: +81-72-820-3095
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Fax +41 44 456 33 30
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P
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