name : current position title

Carole Di Poi Broussard, Ph.D.
Current position:
Postdoctoral Researcher in Marine Ecotoxicology & Ecophysiology
Université de Caen Basse-Normandie
CERMN UPRES EA 4258 - FR CNRS 3038 INC3M
Phone: +33-629-668-274
UMR BOREA; MNHN, UPMC, UCBN, CNRS-7208, IRD-207
Email: [email protected]
Esplanade de la Paix CS14032, F-14032 Caen, Cedex 5
RESEARCH FIELDS
Invertebrate and Fish Ecology • Animal Behavior • Physiology and Neurobiology of Stress
Aquatic Ecotoxicology • Physiology/Biochemistry of Detoxification and Oxidative Stress Regulation
MODEL ORGANISMS
 Invertebrates: freshwater crustacean (e.g., daphnia), marine molluscs (e.g., bivalves, cephalopods)
 Vertebrates: fishes (e.g., sea bass, threespine stickleback), marine mammals (e.g., sea lion, harbor seal)
TECHNICAL SKILLS
 Husbandry: rearing of marine/freshwater fishes and invertebrates, algae cultures.
 Behavioral analysis: field- and laboratory based studies (social and fitness-related behaviors, antipredator
and predator behaviors).
 Organismal bioassays: embryotoxicity and metamorphosis bioassays, immobilization and reproduction
bioassays, physiological responses (e.g., in ovo ventilatory rate in cuttlefish embryos).
 In vivo toxicity bioassays: biomarkers of cell oxidative stress and detoxification (catalase, GST),
peroxidation of membranes (TBARS), neurotransmitter (monoamines, metabolites and receptors) and stress
hormone (cortisol, corticosterone) quantification.
 In vitro toxicity bioassays: cell viability (MTT test), stability of cell membranes (Neutral Red Retention
assay), immunotoxicity (phagocytic activity, ROS production).
 Technical skills: enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), High
Performance Liquid Chromatography (HPLC), flow cytometry, RNA extraction and PCR, qRT-PCR.
 Image analysis: GIMP and ImageJ softwares, Jwatcher (ethograms).
 Bio-statistics: Statview, Statistica, StatXact, R soft
OTHER RELEVANT SKILLS
 Management of research project, grant and funding proposal writing (in French and English)
 Teaching and mentorship
 Languages: English (fluent), French (native language)
EDUCATION
2005-2008
Ph.D. in Neuro-Ethology of Fish
ENES UMR 8195
Université Jean Monnet, Saint-Etienne (France)
Funding: French Ministry of Higher Education and Research
Supervisors: Pr. N. Mathevon and Dr. J. Attia
Grade: Honors with oral congratulations
2004-2005
Master’s degree (2nd yr) in Physiology in Extreme Environments
Université Claude Bernard, Lyon 1 (France)
2005
Training course in Cetology
École Pratique des Hautes Études (EPHE), Montpellier (France)
2003-2004
Master’s degree (1st yr) in Marine Biology and Ecology
Université de la Méditerranée, Marseilles (France)
2002-2003
License’s degree (3rd yr) in Marine Biology and Ecology
Université de la Méditerranée, Marseilles (France)
2000-2002
License’s degree (2 yrs) in Life Sciences
Université Joseph Fourier, Grenoble (France)
RESEARCH EXPERIENCE
January 2015-present
Research fields: Marine Ecotoxicology, Ecophysiology
CERMN UPRES EA 4258 - FR CNRS 3038 INC3M
UMR BOREA; MNHN, UPMC, UCBN, CNRS-7208, IRD-207
Université de Caen Basse-Normandie, Caen (France)
April 2012-March 2014
Research fields: Marine Ecotoxicology, Behavioral Biology,
Ecophysiology
GMPc EA4259
UMR BOREA; MNHN, UPMC, UCBN, CNRS-7208, IRD-207
Université de Caen Basse-Normandie, Caen (France)
Dec. 2010-Dec. 2011
Research field: Behavioral Ecotoxicology, Ecophysiology
GMPc EA4259
Université de Caen Basse-Normandie, Caen (France)
Oct. 2009-Nov. 2010
Research field: Genomics of Behavior
Nadia Aubin-Horth’s Lab
Institute of Integrative and Systems Biology
Laval University, Quebec City (Canada)
Nov. 2008-Sept. 2009
Research fields: Behavioral Ecology, Endocrinology
Shannon Atkinson’s Lab
School of Fisheries and Ocean Sciences
University of Alaska Fairbanks, Juneau (USA)
FACULTY APPOINTMENTS
Sept. 2005-Sept. 2008
Sessional lecturer (242 hrs of teaching in Biology)
Université Jean Monnet, Saint-Etienne (France)
Sept. 2011-June 2012
Sessional lecturer (30 hrs of teaching in Psychophysiology)
Université de Caen Basse-Normandie, Caen (France)
RESEARCH FUNDING, GRANTS & AWARDS
2015-2016
DARM project (Pharmaceuticals, Personal Care Products and
ecotoxicology in marine bivalves) funded by the Région BasseNormandie (principal investigator: Dr. M.-P. Halm UCBN).
(Post-doc salary for 15 months)
2011-2015
Pharm@Ecotox project (Pharmaceutical residues and marine
ecotoxicology) funded by the Agence Nationale de la Recherche ANR
(principal investigator: Dr. M.-P. Halm UCBN).
(Post-doc salary for 24 months)
2010-2011
Postdoctoral Research Fellowship (Antidepressant residues and marine
ecotoxicology in cephalopods) funded by the Université de Caen BasseNormandie - 32.000 euros
(principal investigator)
2010
Travel grant of the Canadian Society of Zoology - US$500
2009-2010
Government of Canada Postdoctoral Research Fellowship (PDRF)
(Behavioral and molecular divergence in threespine stickleback) funded
by the Government of Canada - CAD$32.000
(principal investigator)
2008-2009
Postdoctoral Fellowship (Maternal buffering of stress response in
pinniped pups) funded by the Fyssen Foundation - 25.000 euros
(principal investigator)
2008
Travel grant of the French Society for the Study of Animal Behavior
(SFECA) - 250 euros
2007
Award for Ph.D. Excellence, Women in Sciences Program from L’Oréal
France-UNESCO-French Academy of Science - 10.000 euros
OTHER RESEARCH ACTIVITIES
2013: Participation to the COST FA1301 “A network for improvement of cephalopod welfare and husbandry
in research, aquaculture and fisheries (CephsInAction)”.
2011-2013: Student and Postdoc representative at the GMPc lab council (Université de Caen Basse-Normandie,
Caen, France)
2010: Organization of the 5th Annual Meeting of the Canadian Society for Ecology and Evolution (Quebec City,
Canada)
2005-2008: Participation to the COST 867 WELLFISH “Welfare of Fish in European Aquaculture”
Scientific Journal Reviewer: Biology Letters, Behavioural Brain Research, Aquatic Toxicology, etc.
Member to scientific societies: French Society for the Study of Animal Behavior (SFECA), Association for the
Research in Toxicology (ARET), Society for Experimental Biology (SEB).
LIST OF PUBLICATIONS
 production: 1.6 articles/year
Forthcoming articles
5. Bidel F., Di Poi C. et al. Changes in cuttlefish brain following early postembryonic exposure to Venlafaxine
(antidepressant): Neurodevelopmental and behavioral outcomes. In preparation.
4. Di Poi C., Halm-Lemeille M-.P., Deft P., Budzinski H., Darmaillacq A.-S., Bellanger C. Acute toxicity of the
antidepressant fluoxetine in cuttlefish embryos. In preparation.
3. Di Poi C., Bélanger D., Amyot M., Rogers S., Aubin-Horth N. Evolution of four regulatory molecular
networks associated with behaviour divergence in sticklebacks. Submitted (Current Biology).
2. Bidel F., Le Pabic C., Imarazene B., Koueta N., Di Poi C. et al. Biochemical and predatory behavior changes
in juvenile cuttlefish (Sepia officinalis) following exposure to environmental concentrations of
Venlafaxine (antidepressant). Submitted (Aquatic Toxicology; special issue: Behavioural Aquatic
Toxicology).
1. Di Poi C., Lacasse J., Rogers S., Aubin-Horth N. Habitat-associated evolution of stress reactivity in
sticklebacks. Submitted (Biology Letters).
Published articles
16. Bidel F., Di Poi C., Imarazene B., Koueta N., Budzinski H., Van Delft P., Bellanger B., Jozet-Alves C. In
press. Pre-hatching fluoxetine-induced neurochemical, neurodevelopmental, and immunological changes
in newly hatched cuttlefish. Environmental Science and Pollution Research.
15. Di Poi C., Hoover-Miller A., Blundell G., Atkinson S. 2015. Maternal buffering of stress response in freeranging Pacific harbor seal pups in Alaska. Marine Mammal Science 31(3):1098-1117 (IF: 2.128).
14. Di Poi C., Lacasse J., Rogers S., Aubin-Horth N. 2014. Extensive behavioural divergence following
colonisation of the freshwater environment in threespine sticklebacks. PLOS One 9(6):e98980 DOI:
10.1371/journal.pone.0098980 (IF: 4.09).
13. Di Poi C., Evariste L., Séguin A., Mottier A., Pedelucq J., Lebel J.-M., Serpentini A., Budzinski H., Costil
K. 2014. Sub-chronic exposure to fluoxetine in juvenile oysters (Crassostrea gigas): uptake and
biological effects. Environmental Science and Pollution Research DOI 10.1007/s11356-014-3702-1 (IF:
2.618).
12. Minguez L., Di Poi C., Farcy E., Ballandonne C., Benchouala A., Bojic C., Cossu-Leguille C., Costil K.,
Serpentini A., Lebel JM., Halm-Lemeille M.-P. 2014. Comparison of the sensitivity of seven marine and
freshwater bioassays as regards antidepressant toxicity assessment. Ecotoxicology 23(9):1744-1754 (IF:
2.773).
11. Di Poi C., Bidel F., Dickel L., Bellanger C. 2014. Cryptic and biochemical responses in young cuttlefish
Sepia officinalis exposed to environmentally relevant concentrations of fluoxetine. Aquatic Toxicology
151:36-45 (IF: 3.761).
10. Di Poi C., Bellanger C. 2014. Response to commentary on “Are some invertebrates exquisitely sensitive to
the human pharmaceutical fluoxetine?”. Aquatic Toxicology 146:261-263 (IF: 3.730).
9. Di Poi C., Evariste L., Serpentini A., Halm-Lemeille M.-P., Lebel J.-M., Costil K. 2014. Toxicity of five
antidepressant drugs on the embryo-larval development and metamorphosis in the Pacific oyster,
Crassostrea gigas. Environmental Science and Pollution Research 21(23):13302-13314 (IF: 2.618).
8. Di Poi C., Darmaillacq A.-S., Dickel L., Boulouard M., Bellanger C. 2013. Effects of perinatal exposure to
waterborne fluoxetine on memory processing in the cuttlefish Sepia officinalis. Aquatic Toxicology 132133:84-91 (IF: 3.761).
7. Di Poi C. 2012. The lament of Alaskan harbor seal pups: a story about maternal support. Annales de la
Fondation Fyssen 27:62-77.
6. Attia J., Millot S., Di Poi C., et al. 2012. Demand feeding and welfare in farmed fish. Fish Physiology and
Biochemistry 38:107-118 (IF: 1.607).
5. Di Poi C., et al. 2011. Biodiversity matters in a changing world. Biology Letters 7:4-6 (IF: 3.521).
4. Das K., Siebert U., Gillet A., Dupont A., Di Poi C., et al. 2008. Mercury immune toxicity in harbour seals:
links to in vitro toxicity. Environmental Health 7:52 (IF: 2.480).
3. Millot S., Bégout M.-L., Person J., Breuil G., Di Poi C., et al. 2008. Feed demand behavior in sea bass
juveniles: effects on individual specific growth rate variation and health (inter-individual and inter-group
variation). Aquaculture 274:87-95 (IF: 1.925).
2. Di Poi C., et al. 2008. Effects of high food-demand fish removal in groups of juvenile Sea bass
(Dicentrarchus labrax). Canadian Journal of Zoology 86:1015-1023 (IF: 1.254).
1. Di Poi C., et al. 2007. Behavioral and neurophysiological responses of European sea bass groups reared
under food constraint. Physiology & Behavior 90:559-566 (IF: 3.295).
COMMUNICATIONS
Invited talks
2. Di Poi C. 2014. Sub-lethal effects of psychotropic drugs on aquatic organisms: linking behaviour to
neurobiological correlates. Public seminar at Lakehead University in the framework of a job interview to
a Canada Research Chair position (Thunder Bay, Canada).
1. Di Poi C. 2013. The Study of individual variability in feeding behaviour and its structuration: Examples in
Fish and Cephalopods. French Society for the Study of Animal Behaviour (Dijon, France).
Conference talks
23. Di Poi C., Minguez L., Costil K., Lebel J.-M., Budzinsky H., Bureau R., Bidel F., Bellanger C., CossuLeguille C., Serpentini A., Halm M.-P. 2015. Pharm@ecotox: Impact écotoxicologique des résidus
pharmaceutiques au niveau dulcicole et marin. SEFA (Bordeaux, France).
22. Bidel F., Bellanger C., Boulourd M., Darmaillacq A-S., Dickel L., Di Poi C., Jozet-Alves C., Olley R. 2015.
Antidepressant exposure during early postembryonic development modulates brain plasticity, brain
architecture and behaviours in cuttlefish (Sepia officinalis). PRIMO18 (Trondheim, Norvège).
21. Minguez L., Di Poi C., Costil K., Lebel J.-M., Budzinsky H., Bureau R., Bidel F., Bellanger C., CossuLeguille C., Serpentini A., Halm M.-P. 2015. Pharm@ecotox : Impact écotoxicologique des résidus
pharmaceutiques au niveau dulcicole et marin. ECOBIM (Québec, Canada).
20. Bidel F., Di Poi C., Arini A., Basu N., Boulouard M., Darmaillacq A-S., Dickel L., Olley R., Jozet-Alves
C., Bellanger C. 2014. Venlafaxine exposure during early postembryonic development is associated with
neurochemical and behavioral changes in cuttlefish (Sepia officinalis). SETAC (Vancouver, Canada).
19. Bellanger C., Di Poi C., Bidel B., Jozet C., Dickel L., Boulouard M., Darmaillacq A.-S. 2014. Behavioural
and cerebral changes occuring in the cuttlefish with perinatal exposure to antidepressant. 11th
International Congress of Neuroethology (Sapporo, Japan).
18. Séguin A., Di Poi C., Evariste L., Mottier A., Serpentini A., Lebel J.-M., Costil K. 2014. Effets des
antidépresseurs sur les jeunes stades de l’huître creuse, Crassostrea gigas. ECOBIM (Plouzané, France).
17. Di Poi C., Bidel F., Darmaillacq A.-S., Jozet C., Dickel L., Bellanger C. 2014. Brain and behaviour
ontogenesis of the cuttlefish: impact of environmental pollution by pharmaceutical residues. COST
FA1301 (Barcelona, Spain).
16. Bellanger C., Di Poi C. 2013. Etude écotoxicologique des psychotropes sur un céphalopode : approches
comportementale et neurobiologique. Workshop « Les médicaments dans l’environnement » (Albi,
France).
15. Di Poi C., Darmaillacq A.-S., Boulouard M., Bellanger C. 2013. Psychotropic drugs as behavioural
disruptors in a non-target marine organism: What does the cuttlefish on Prozac tell us? Society for
Experimental Biology's Annual Meeting (Valencia, Spain).
14. Di Poi C., Evariste L., Serpentini A., Halm-Lemeille M.-P., Lebel J.-M., Costil K. 2013. Toxicity of five
antidepressant drugs on the embryo-larval development in the Pacific oyster, Crassostrea gigas.
PRIMO17 Pollutant Responses in Marine Organisms (Faro, Portugal).
13. Di Poi C., Lacasse J., Amyot M., Rogers S., Aubin-Horth N. 2012. Marine and freshwater sticklebacks
differ in behaviours and stress response: link with neurogenomic state. 7th International Conference on
Stickleback Behavior and Evolution (Seattle, USA).
12. Di Poi C., Lacasse J., Rogers S., Aubin-Horth N. 2012. Behavioural and neurogenomic divergence between
freshwater and marine threespine stickleback populations. 1st Joint Congress on Evolutionary Biology
(Ottawa, Canada).
11. Di Poi C., Aubin-Horth N. 2011. The « Evolutionary History of temperament » told by the threespined
stickleback. French Society for the Study of Animal Behaviour (Tours, France).
10. Di Poi C., Rogers S., Aubin-Horth N. 2010. Antipredator behavior in three-spined stickleback Gasterosteus
aculeatus : Are freshwater fish more bold than their marine ancestor ? 5th Annual Canadian Society of
Ecology and Evolution Meeting (Quebec City, Canada).
9. Di Poi C. 2010. À quel point le contexte social influence les comportements individuels et la réponse au
stress chez les Vertébrés? Interlabs-IBIS (Quebec City, Canada).
8. Di Poi C., Atkinson S., Hoover-Miller A., Blundell G. 2010. The presence of the mother influences the stress
response in Alaskan harbor seal pups Phoca vitulina. Annual Meeting of the Society for Integrative and
Comparative Biology (Seattle, USA).
7. Di Poi C., Aubin-Horth N. 2009. Test du tempérament : en quoi les fonceurs et les timides sont-ils
différents ? Un exemple chez les poissons. 1er Symposium des Études Postdoctorales à l’Université Laval
(Quebec city, Canada).
6. Di Poi C., Attia J., Mathevon N., Beauchaud M. 2008. Individual recognition affects AVT expression in
brain of European sea bass (Dicentrarchus labrax). 4th European Conference on Behavioral Biology
(Dijon, France). Granted by the French Society for the study of Animal Behavior (SFECA).
5. Di Poi C., Beauchaud M., Schrama J., Begout M.-L., Attia J. 2008. Analysis of the self-feeding behavior in
European sea bass juveniles using X-radiography. 9th day of Cytometry (Saint-Etienne, France).
4. Di Poi C., Beauchaud M., Schrama J., Begout M.-L., Attia J. 2008. Monitoring of the self-feeding behavior
in European sea bass juveniles using X-radiography: Analysis of feed intake and feed efficiency. COST
867 « WellFish » (Krakow, Poland).
3. Di Poi C., Millot S., Attia J., Bégout M.-L., Beauchaud M. 2007. Social dynamic of group feeding in sea bass
juveniles. COST 867 « WellFish » (Varese, Italy).
2. Di Poi C., Millot S., Beauchaud M., Dutto G., Covès D., Bégout M.-L., Attia J. 2006. Voluntary food
demand in the sea bass, Dicentrarchus labrax: Behavioral and Neuro-physiological approaches. 3rd
French Conference of Ichtyology (Paris, France).
1. Millot S., Di Poi C., Person-Le-Ruyet J., Sévère A., Pineau P., Bégout M.-L. 2006. Long- term analysis of
the food demand in sea bass (Dicentrarchus labrax) populations reared in self-feeding conditions : Impact
of the individually triggering activity on the group feeding. Oral contribution, 3rd French Conference of
Ichtyology (Paris, France).
Posters
14. Atkinson S., Di Poi C., Hoover-Miller A., Blundell G. 2014. Free-ranging harbor seal pups experience
maternal buffering of stress response. Alaska Marine Science Symposium (Anchorage, USA).
13. Bidel F., Bellanger C., Di Poi C., Jozet-Alves C. 2013. May marine pollution by psychotropic drugs impact
the chance of survival in young cuttlefish (Sepia officinalis). Society for Experimental Biology's Annual
Meeting (Valencia, Spain).
12. Boulouard M., Bellanger C., Darmaillacq A.-S., Dickel L., Di Poi C. 2013. Effects of perinatal exposure to
waterborne fluoxetine on memory processing in the cuttlefish Sepia officinalis. 11e Colloque de la Société
des Neurosciences (Lyon, France).
11. Di Poi C., Boulouard M., Darmaillacq A.-S., Bellanger C. 2013. Behavioural toxicity of the antidepressant
fluoxetine in the common cuttlefish Sepia officinalis. PRIMO17 Pollutant Responses in Marine
Organisms (Faro, Portugal).
10. Di Poi C., Le Pabic C., Darmaillacq A.-S., Koueta N., Bellanger C. 2012. Effects of waterborne fluoxetine
on survival behaviors and physiological functions in young cuttlefish. Cephalopod International Advisory
Council Symposium (Florianópolis, Brazil).
9. Bellanger C., Paniez E., Darmaillacq A.S., Boulouard M., Di Poi C. 2012. Fluoxetine exposure and
development of fundamental behaviors and learning in a cephalopod (cuttlefish). 8th FENS Forum of
Neuroscience (Barcelona, Spain).
8. Di Poi C., Paniez E., Darmaillacq A.S., Boulouard M., Bellanger C. 2012. Cuttlefish on Prozac: How
depressing? Société Française pour l’Étude du Comportement Animal (Saint-Etienne, France).
7. Di Poi C., Aubin-Horth Nadia. 2010. Test of personality: Into the head of bold and shy three-spined
sticklebacks. The Society for Behavioral Neuroendocrinology Annual Meeting (Toronto, Canada).
6. Di Poi C., Maniscalco J. M., Parker P., Atkinson S. 2009. A behavioral assessment of Steller sea lion
(Eumotopias jubatus) pups exposed to hot branding: Do branded pups show greater signs of stress
compared to unbranded pups? The 18th SMM Biennial Conference on the Biology of Marine Mammals
(Québec City, Canada).
5. Di Poi C., Attia J., Bouchut C., Dutto G., Covès D., Beauchaud M. 2007. Behavioral and neurophysiological
responses of European sea bass groups reared under food constraint. XXX International Ethological
Conference (Halifax, Canada).
4. Di Poi C., Attia J., Bouchut C., Dutto G., Covès D., Beauchaud M. 2007. “Who leaves his place loses it”:
Social knockout effect in captive groups of juvenile European sea bass. XXX International Ethological
Conference (Halifax, Canada).
3. Di Poi C., Attia J., Beauchaud M. 2006. Behavioral and neurophysiological responses of European sea bass
groups reared under controlled environment. French and Chinese young scientists meeting (Bejing,
China).
2. Di Poi C., Attia J., Beauchaud M., Bouchut C., Covès D., Dutto G., Mathevon N. 2006. « Qui va à la chasse
perd sa place! » Hierarchy and social group in the Mediterranean sea bass. Research day of the Doctoral
School of Saint-Etienne (Saint-Etienne, France).
1. Di Poi C., Siebert U., Drouguet O., Fonfara S., Lepoint G., Das K. 2005. Stable isotopes and mercury in
blood of Harbour seals (Phoca vitulina) caught on a German sandbank. 19th Conference of the European
Cetacean Society (La Rochelle, France).
TEACHING EXPERIENCE
Table summarizing my experience as a sessional lecturer in French universities (Université Jean Monnet, SaintEtienne and Université de Caen Basse-Normandie, Caen).
Courses
Number of hours
Lectures
Animal Physiology and
Neuroanatomy
Haploid Genetic
Developmental Biology
Zoology
Cell Biology
Biochemistry
Psychophysiology
Neuro-ethology
Practical
classes
4
Degrees
Laboratory
classes
99
52
25
11
8
8
12
18
TOTAL
40
Undergraduate
(Licence’s degree)
Graduate
(Master’s degree)
277 hours
TRAINING & SUPERVISORY EXPERIENCE
2005-2015
Supervision or co-supervision of Licence and Master’s students
(9 Licences; 13 Masters)
OUTREACH ACTIVITIES
2009-2012-2013
2010
2009
2006
Scientific animation at the « Fête de la science » (celebration day for sciences in
France).
Scientific representative for teenagers during the «Girls and Science» day
(Quebec City, Canada).
Science judge for the Alaska Tsunami Ocean Sciences Bowl (Seward, USA)
Participation in the program « A Scientist in High-School ». The objective of the
program is to introduce Sciences to children (12-15 years old).