OIE Reference Laboratory Reports Activities Activities in 2014

Transcription

OIE Reference Laboratory Reports Activities Activities in 2014
OIE Reference Laboratory Reports Activities
Activities in 2014
This report has been submitted : 2015-01-23 21:30:20
Name of disease (or topic) for which
you are a designated OIE Reference
Laboratory:
Bovine tuberculosis
Address of laboratory:
Unité des zoonoses bactériennes (ACI-ROU-009), Laboratoire
de Santé Animale, Anses,14 rue Pierre et Marie Curie, 94701
Maisons-Alfort, FRANCE
Tel.:
+33 (0)1 49 77 13 50
Fax:
+33 (0)1 49 77 13 44
E-mail address:
[email protected]
Website:
Name (including Title) of Head of
Laboratory (Responsible Official):
Dr. Pascal Boireau, Head of the Animal Health Laboratory,
Anses, Maisons Alfort
Name (including Title and Position)
of OIE Reference Expert:
Dr. María Laura Boschiroli-Cara, Research Director, head of the
Tuberculosis National Reference Laboratory
Which of the following defines your
laboratory? Check all that apply:
Governmental
OIE Reference Laboratory Reports Activities, 2014
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Bovine tuberculosis - María Laura Boschiroli-Cara - france
ToR 1: To use, promote and disseminate diagnostic methods validated according to
OIE Standards
1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease
diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff
training)
Yes
Diagnostic Test
Indicated in OIE Manual
(Yes/No)
Indirect diagnostic tests
Total number of test performed last year
Nationally
Internationally
IFNg test
Y
40
0
Serology (cattle)
N
80
0
Serology (wild boar)
N
40
0
tuberculine batch control
Y
14
3
Nationally
Internationally
Direct diagnostic tests
bacteriology
Y
438
45
strains identification
Y
1065
12
PCR
Y
1742
45
spoligotyping
Y
772
2
MLVA
Y
149
0
ToR 2: To develop reference material in accordance with OIE requirements, and
implement and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and
any other reagents used in the diagnosis and control of the designated pathogens
or disease.
2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE?
No
3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents
to OIE Member Countries?
2
OIE Reference Laboratory Reports Activities, 2014
Bovine tuberculosis - María Laura Boschiroli-Cara - france
No
4. Did your laboratory produce vaccines?
No
5. Did your laboratory supply vaccines to OIE Member Countries?
No
ToR 3: To develop, standardise and validate, according to OIE Standards, new
procedures for diagnosis and control of the designated pathogens or diseases
6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated
pathogen or disease?
No
7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?
No
ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific
and technical advice on disease control measures to OIE Member Countries
8. Did your laboratory carry out diagnostic testing for other OIE Member Countries?
No
9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?
Yes
Name of the OIE Member Country
receiving a technical consultancy
Purpose
How the advice was provided
IRELAND
Achievement of a
vaccination trial for wildlife
bacteriology and PCR on samples
from vaccinated badgers
ToR 5: To carry out and/or coordinate scientific and technical studies in
collaboration with other laboratories, centres or organisations
10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries
other than the own?
OIE Reference Laboratory Reports Activities, 2014
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Bovine tuberculosis - María Laura Boschiroli-Cara - france
Yes
Title of the study
Protective efficacy of heat
inactivated M. bovis as an
oral vaccine against
tuberculosis in European
badgers
Duration
Purpose of the study
Partners (Institutions)
6 months
Evaluation of the efficacy
of an inactivated M. bovis
strain in badgers for
vaccination
Neiker (Spain), IREC (Spain),
University of Dublin (Ireland),
AHVLA (UK), INRA (France), Anses
(France), University College of
Dublin (Ireland)
ToR 6: To collect, process, analyse, publish and disseminate epizootiological data
relevant to the designated pathogens or diseases
11. Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
12. Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
13. What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a) Articles published in peer-reviewed journals: 11
Biet F, Boschiroli ML. 2014. Non-Tuberculous Mycobacterial infections of veterinary relevance Research in
Veterinary Science 97:S69-S77. doi:DOI: 10.1016/j.rvsc.2014.08.007.
Boschiroli ML, Bénet JJ. 2014. Bovine tuberculosis eradication in France, p. 341-347. In Thoen CO, Steele JH,
Kaneene JB (ed.), Zoonotic tuberculosis: Mycobacterium bovis and other pathogenic mycobacteria -Third edition.
Wiley Blackwell, West Sussex, UK.
Courcoul A, Moyen JL, Brugère L, Faye S, Hénault S, Gares H, Boschiroli ML. 2014. Estimation of sensitivity and
specificity of bacteriology, histopathology and PCR for the confirmatory diagnosis of bovine tuberculosis using
latent class analysis. PloS one 9:e90334. doi:10.1371/journal.pone.0090334.
Praud A, Boschiroli ML, Meyer L, Garin-Bastuji B, Dufour B. 2014. Assessment of the sensitivity of the gammainterferon test and the single intradermal comparative cervical test for the diagnosis of bovine tuberculosis under
field conditions. Epidemiology and infection:1-10. doi:10.1017/S0950268814000338.
Courcoul A, Moyen J-L, Brugère L, Faye S, Hénault S, Gares H, Boschiroli M-L. 2014. Evaluation des
caractéristiques des test de confirmation de la tuberculose bovine : Comparaison de la culture bactérienne, de
l'histopathologie et de la PCR. Epidemiologie et santé animale 65:21-29.
Hauer A, Cochard T, De Cruz K, Karoui C, Henault S, Biet F, Boschiroli M-L. 2014. Comparaison des souches de
Mycobacterium bovis isolées en Frace en élévage et dans la faune sauvage grâce aux techniques d'identification
moléculaire. Epidemiologie et santé animale 65:77-86.
Keck N, Moyen JL, Gueneau E, Boschiroli ML. 2014. Particularités du dépistage et du diagnostic de la Tuberculose
Bovine. Epidemiologie et santé animale 65:5-19.
Moyen JL, Gueneau E, Keck N, Gares H, Boschiroli ML. 2014. Premières évaluations de l'intérêt de la sérologie
pour le diagnostic de la tuberculose bovine en France. Epidemiologie et santé animale 65:41-51.
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OIE Reference Laboratory Reports Activities, 2014
Bovine tuberculosis - María Laura Boschiroli-Cara - france
Payne A, Hars J, Fossot C, Barbier E, Hartmann A, Boschiroli ML, Gueneau E, Dufour B, Gilot-Fromont E. 2014. Rôle
de populations sauvages dans le système multi-hôtes de Mycobacterium bovis en Côte-d'Or: Evaluation du risque
de transmission-retour aux bovins et du risque de constitution de réservoir sauvage. Epidemiologie et santé
animale 65:133-150.
Richomme C, Boadella M, Courcoul A, Durand B, Drapeau A, Corde Y, Hars J, Payne A, Fediaevsky A, Boschiroli ML.
2014. Utilisation d'un outils sérologique pour estimer l'expositiondes sangliers à M. bovis. Epidemiologie et santé
animale 65:151-161.
Fediaevsky A, Courcoul A, Boschiroli ML, Reveillaud E. 2014. Tuberculose bovine en France en 2013 : résultats
d’une stratégie plus offensive. Bulletin Epidémiologique Santé Animale-Alimentation Spécial Maladies
Réglementées et Emergentes (MRE):4-11.
b) International conferences: 11
De Cruz K, Karoui C, Bulach T, Hénault S, Moyen JL, Boschiroli ML. 2014. Non Tuberculous Mycobacteria
Generating False Positive Results In TB Diagnostics in France, VI International M.bovis Conference, Cardiff, Wales.
Fediaevsky A, Courcoul A, Desvaux S, Chevalier F, Jabert P, Alvado-Brette MB, Tourette I, Philizot S, Keck N, Hars J,
Dufour B, Garin E, Boschiroli ML, Reveillaud E, Hendrikx P, Gueriaux D, Calavas D. 2014. Strengthening
Surveillance Of Bovine Tuberculosis, The Experience Learned From A Public Private Sector Initiative, The
Plateforme ESA, VI International M.bovis Conference, Cardiff, Wales.
Hauer A, Biet F, Boschiroli ML. 2014. First genomic data of Mycobacterium bovis strains isolated from animals in
France, Microbiology after the genomics revolution – Genomes 2014, Paris, France.
Hauer A, Cochard T, De Cruz K, Karoui C, Henault S, Biet F, Boschiroli ML. 2014. Demography and Molecular
Diversity Of Mycobacterium bovis Strains Isolated In France From 1978 to 2011, VI International M.bovis
Conference, Cardiff, Wales.
Hauer A, Cochard T, De Cruz K, Karoui C, Hénault S, Biet F, Boschiroli ML. 2014. Discriminatory Power Of
Spoligotyping and MLVA In French M. bovis Strains, VI International M.bovis Conference, Cardiff, Wales.
Hénault S, De Cruz K, Bulach T, Karoui C, Brugère L, Gares H, Moyen JL, Gueneau E, Keck N, Courcoul A,
Fediaevsky A, Boschiroli ML. 2014. First line Molecular Diagnosis Of Bovine Tuberculosis: The French Experience,
VI International M.bovis Conference, Cardiff, Wales.
Lesellier S, Richomme C, Barrat J, Boschiroli ML, Gortazar C, Juste RA, Sevilla IA, Garrido JM, Anderson P, Dave D,
Palmer S, Ni Bhuachalla D, Salguero FJ, Spiropoulos J, Sarradin P, Barc C, Pleau A, Gautier D, Pinard A, Carreras F,
Adriaensen H, Lecompte F, Chambers M, Gormley E. 2014. Protective Efficacy Of An Oral Heat-inactivated M.
bovis Vaccine For Badgers Against Bovine Tuberculosis - A Pilot Study, VI International M.bovis Conference,
Cardiff, Wales.
Michelet L, De Cruz K, Bulach T, Karoui C, Hénault S, Boschiroli ML. 2014. Surveillance Of Mycobacterium microti
In Animals, France, 2002-2014, VI International M.bovis Conference, Cardiff, Wales.
Moyen JL, Gueneau E, Keck N, Gares H, Boschiroli ML. 2014. Use Of A Serological Test For Bovine Tuberculosis In
France, VI International M.bovis Conference, Cardiff, Wales.
Moyen JL, Holbert S, Boschiroli ML. 2014. Adjustments and Controls In The Use Of Bovigam B1G To Improve The
Reproducibility, VI International M.bovis Conference, Cardiff, Wales.
Reveillaud E, Hars J, Richomme C, Boschiroli ML, Faure E, Chollet J-Y, Hendrikx P, Fediaevsky A. 2014. SYLVATUB:
A Bovine Tuberculosis Surveillance Network In Wildlife In France, VI International M.bovis Conference, Cardiff,
Wales.
c) National conferences: 4
Richomme C, Courcoul A, Durand B, Boschiroli ML. 2014. Relation entre l’exposition à Mycobacterium bovis chez
les sangliers et la distribution des foyers de tuberculose dans les élevages bovins, Rencontres scientifiques de
l'ANSES, Maisons-Alfort.
Boschiroli ML. 2014. Diagnostic et surveillance de la tuberculose bovine en France : la guerre est déclarée !,
SPACE, Rennes.
OIE Reference Laboratory Reports Activities, 2014
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Bovine tuberculosis - María Laura Boschiroli-Cara - france
Hauer A, Cochard T, De Cruz K, Karoui C, Henault S, Biet F, Boschiroli ML. 2014. Molecular identification of
Mycobacterium bovis strains of responsible of bovine tuberculosis outbreaks in France, in livestock and in wildlife,
Journée de Doctorants de l'INRA, Tours.
Hauer A, Biet F, Boschiroli ML. 2014. Analyse génomique des différents génotypes de Mycobacterium bovis
circulants en France Journée des Doctorants de l'ANSES, Maisons-Alfort.
d) Other:
(Provide website address or link to appropriate information) 0
ToR 7: To provide scientific and technical training for personnel from OIE Member
Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member
Countries?
Yes
a) Technical visits: 0
b) Seminars: 0
c) Hands-on training courses: 1
d) Internships (>1 month): 0
Type of technical training provided
(a, b, c or d)
Country of origin of the
expert(s) provided with training
No. participants from the
corresponding country
Hands-on training course
Korea
1
ToR 8: To maintain a system of quality assurance, biosafety and biosecurity
relevant for the pathogen and the disease concerned
15. Does your laboratory have a Quality Management System certified according to an International Standard?
Yes
Quality management system adopted
Certificate scan (PDF, JPG, PNG format)
ISO 17025
1-2246.pdf
16. Is your laboratory accredited by an international accreditation body?
No
17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned?
Yes
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OIE Reference Laboratory Reports Activities, 2014
Bovine tuberculosis - María Laura Boschiroli-Cara - france
(See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a)
ToR 9: To organise and participate in scientific meetings on behalf of the OIE
18. Did your laboratory organise scientific meetings on behalf of the OIE?
No
19. Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
Title of event
Primer encuento interamericano brucelosis
y tuberculosis en el
mundo animal
Date
(mm/yy)
20/11/2014
Location
Montpellier,
France
Role (speaker,
presenting poster,
short
communications)
Title of the work
presented
speaker
TB, the French
approach •
Comparisons
amongst
methodologies
ToR 10: To establish and maintain a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organise regular inter-laboratory
proficiency testing to ensure comparability of results
20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same
pathogen or disease?
Yes
21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the
same pathogen or disease by organising or participating in proficiency tests?
No
22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific
research projects for the diagnosis or control of the pathogen of interest?
Yes
OIE Reference Laboratory Reports Activities, 2014
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Bovine tuberculosis - María Laura Boschiroli-Cara - france
Title of the project or contract
Scope
Name(s) of relevant OIE Reference
Laboratories
Protective efficacy of heat inactivated M.
bovis as an oral vaccine against
tuberculosis in European badgers
control of badger
disease and then
spillover to cattle
bovine tuberculosis OIE reference
laboratory, AHVLA, GB.
ToR 11: To organise inter-laboratory proficiency testing with laboratories other
than OIE Reference Laboratories for the same pathogens and diseases to ensure
equivalence of results
23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than
OIE Reference Laboratories for the same disease?
Yes
Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Purpose for inter-laboratory test comparisons1
DVR-spoligotyping (organised by the bovine
tuberculosis EURL)
Biological test of tuberculins (organised by the
bovine tuberculosis EURL)
Direct extraction and microbiological culture
(organised by the bovine tuberculosis EURL)
Direct extraction and microbiological culture
(organised by our laboratory)
8
No. participating
laboratories
Region(s) of participating
OIE Member Countries
unknown (ongoing)
Africa
Americas
Asia and Pacific
Europe
Middle East
unknown (ongoing)
Africa
Americas
Asia and Pacific
Europe
Middle East
unknown (ongoing)
Africa
Americas
Asia and Pacific
Europe
Middle East
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Africa
Americas
Asia and Pacific
Europe
Middle East
OIE Reference Laboratory Reports Activities, 2014
Bovine tuberculosis - María Laura Boschiroli-Cara - france
ToR 12: To place expert consultants at the disposal of the OIE
24. Did your laboratory place expert consultants at the disposal of the OIE?
Yes
Kind of consultancy
Location
Subject (facultative)
OIE ad hoc group
OIE Headquater
revision of code chapter for animal tuberculosis
25. Additional comments regarding your report:
OIE Reference Laboratory Reports Activities, 2014
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