OIE Reference Laboratory Reports Activities Activities in 2015

OIE Reference Laboratory Reports Activities
Activities in 2015
This report has been submitted : 2016-02-01 18:04:09
Name of disease (or
topic) for which you are a
designated OIE Reference
Laboratory:
Q fever
Address of laboratory:
Anses (Agence nationale de sécurité sanitaire de l’alimentation, de
l’environnement et du travail), Laboratoire de Sophia-Antipolis, Unité Fièvre
Q Animale, 105, route des Chappes, 06410 BIOT, FRANCE
Tel.:
+33-4 92.94.37.00
Fax:
+33-4 92.94.37.01
E-mail address:
[email protected]
Website:
https://www.anses.fr/fr/content/laboratoire-de-sophia-antipolis
Name (including Title) of
Head of Laboratory
(Responsible Official):
Richard Thiéry
Name (including Title and
Position) of OIE
Reference Expert:
Elodie Rousset
Which of the following
defines your laboratory?
Check all that apply:
Governmental
OIE Reference Laboratory Reports Activities, 2015
1
Q fever - Elodie Rousset - france
ToR 1: To use, promote and disseminate diagnostic methods validated according to
OIE Standards
1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease
diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff
training)
Yes
Diagnostic Test
Indicated in OIE Manual
(Yes/No)
Indirect diagnostic tests
Total number of test performed last year
Nationally
Internationally
ELISA sur sérum caprin, ovin, bovin
Oui (en noir)
11802
0
ELISA sur sérum multi-espece (mise
Non
415
0
Nationally
Internationally
Direct diagnostic tests
PCR Temps Reel Quantitative
Oui (en noir)
1473
6
Génotypage MLVA
Non
41
0
PCR / MLVA (17 marqueurs)
Non
587
0
Isolement (souris, culture cellulaire)
Oui (en noir)
6
0
ToR 2: To develop reference material in accordance with OIE requirements, and
implement and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and
any other reagents used in the diagnosis and control of the designated pathogens
or disease.
2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE?
No
3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents
to OIE Member Countries?
Yes
2
OIE Reference Laboratory Reports Activities, 2015
Q fever - Elodie Rousset - france
Type of
reagent
available
Bactéries
purifiées
inactivées
dosées (Nine
Mile
Standard
ADN
génomique
dosé (Nine
Mile
Sérum
calibrant
Sérum étalon
Related
diagnostic
test
PCR
PCR,
Génotypage
ELISA
(sérologie)
FC
Produced/
provide
Produit
Produit
Produit
Produit
Amount
supplied
nationally
(ml, mg)
14
0.7
27.2
2,5
Amount
supplied
internationally
(ml, mg)
1
0.1
2.8
0
No. of
recipient
OIE
Member
Countries
Region of
recipients
2
Africa
Americas
Asia and
Pacific
Europe
Middle
East
2
Africa
Americas
Asia and
Pacific
Europe
Middle
East
4
Africa
Americas
Asia and
Pacific
Europe
Middle
East
1
Africa
Americas
Asia and
Pacific
Europe
Middle
East
4. Did your laboratory produce vaccines?
No
5. Did your laboratory supply vaccines to OIE Member Countries?
No
ToR 3: To develop, standardise and validate, according to OIE Standards, new
procedures for diagnosis and control of the designated pathogens or diseases
6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated
pathogen or disease?
No
OIE Reference Laboratory Reports Activities, 2015
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Q fever - Elodie Rousset - france
7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?
No
ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific
and technical advice on disease control measures to OIE Member Countries
8. Did your laboratory carry out diagnostic testing for other OIE Member Countries?
No
9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?
Yes
Name of the OIE
Member Country
receiving a
technical
consultancy
Purpose
How the advice was provided
AUSTRALIA
recommandation de kits ELISA pour
porcins
explications par e-mail
LATVIA
support pour la mise en place d'une
PCR, recommandations pour
recherche sur lait, demande de MR
échanges par e-mail (supports, notamment
tableau avec objectif méthodes du chapitre
oie version 2015), discussion sur site,
fourniture de MR
LATVIA
recommandations en sérologie
(antigène CFT, CFT et ELISA, des
contoles, une validation)
renseignements fournis avec proposition du
MR calibrant Elisa, et proposition d'un essai
d'aptitude bilateral
TUNISIA
A la recherche d'un témoin positif de
PCR ciblant Coxiella burnetii afin de
réaliser des PCR
proposition des MR ADN et Bact pour PCR,
renseignements sur les certificats, articles
transmis pour présenter les 2 méthodes
commerciales validées
AUSTRIA
demande de MR et de participation à
une EILA pour sérologie et conseils
proposition des MR, explication
recommandation ELISA et FC, et proposition
d'un essai d'aptitude bilateral
ToR 5: To carry out and/or coordinate scientific and technical studies in
collaboration with other laboratories, centres or organisations
10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries
other than the own?
No
4
OIE Reference Laboratory Reports Activities, 2015
Q fever - Elodie Rousset - france
ToR 6: To collect, process, analyse, publish and disseminate epizootiological data
relevant to the designated pathogens or diseases
11. Did your Laboratory collect epizootiological data relevant to international disease control?
No
12. Did your laboratory disseminate epizootiological data that had been processed and analysed?
No
13. What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a) Articles published in peer-reviewed journals: 9
Duron Olivier, Noël Valérie, D. McCoy Karen, Bonazzi Matteo, Sidi-Boumedine Karim, Morel Olivier, Vavre Fabrice,
Zenner Lionel, Jourdain Elsa, Durand Patrick, Arnathau Céline, Renaud François, Trape Jean-François, Biguezoton
Abel S., Cremaschi Julie, Dietrich Muriel, Léger Elsa, Appelgren Anaïs, Dupraz Marlène, Gómez-Díaz Elena, Diatta
Georges, Dayo Guiguigbaza-Kossigan, Adakal Hassane, Zoungrana Sébastien, Vial Laurence, Chevillon Christine.
2015. The recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the Q fever
pathogen, Coxiella burnetii. PLOS Pathogens. 11(5): e1004892.
Duron Olivier, Sidi-Boumedine Karim, Rousset Elodie, Moutailler Sara, Jourdain Elsa. 2015. The importance of ticks
in Q fever transmission: what has (and has not) been demonstrated. Trends in Parasitology. 31(11):536-522.
Joulié A., Laroucau K., Bailly X., Prigent M., Gasqui P., Lepetitcolin E., Blanchard B., Rousset E., Sidi-Boumédine K.,
Jourdain E. 2015. Circulation of Coxiella burnetii in a naturally infected flock of dairy sheep: shedding dynamics,
environmental contamination, and genotype diversity. Appl Environ Microbiol 81(20):7253-60. doi:
10.1128/AEM.02180-15.
Jourdain Elsa, Duron Olivier, Barry Séverine, González-Acuña Daniel, Sidi-Boumedine Karim. 2015. Molecular
methods routinely used to detect Coxiella burnetii in ticks cross-react with Coxiella-like bacteria. Infection Ecology
and Epidemiology. 5: 29230.
Pimenta L., Alegria N., Anastácio S., Sidi-Boumedine K., da Silva G., Rabiço Â., Simões J. 2015. Prevalence of
Coxiella burnetii antibodies in Portuguese dairy cattle herds. Tropical Animal Health Production. 47(1):227-30.
Prigent Myriam, Rousset Elodie, Yang Elise, Thiery Richard, Sidi-Boumedine Karim. 2015. Validation study for
using lab-on-chip technology for Coxiella burnetii multi-locus-VNTR-analysis (MLVA) typing: application for
studying genotypic diversity of strains from domestic ruminants in France. Microbes and Infection. 17:782-788
http://dx.doi.org/10.1016/j.micinf.2015.09.026
Rosières Xavier, Rautureau Séverine, Rousset Elodie, Klotz Stéphane, Boulogne Ophélie, Deltour Benjamin. 2015.
Cas humains groupés de fièvre Q dans la région Provence-Alpes-Côte d’Azur (Clustered human cases of Q fever in
the PACA region). Bulletin Epidémiologique de l'ANSES et la DGAl. 69 :8-10.
Sidi-Boumedine Karim, Duquesne Véronique, Prigent Myriam, Yang Elise, Joulié Aurélien, Thiéry Richard, Rousset
Elodie. Impact of IS1111 insertion on the MLVA genotyping of C. burnetii. Microbes and Infection.
17(11-12):789-94. doi: 10.1016/j.micinf.2015.08.009.
Sidi-Boumedine Karim, Adam Gilbert, Angen Øysten, Aspán Anna, Bossers Alex, Roest Hendrik-Jan, Prigent
Myriam, Thiéry Richard, Rousset Elodie. 2015. Whole genome PCR scanning (WGPS) of C. burnetii strains from
ruminants. Microbes and Infection. 17(11-12):772-5. doi: 10.1016/j.micinf.2015.08.003.
b) International conferences: 7
Communications affichées
Joulié Aurélien, Laroucau Karine, Bailly Xavier, Prigent Myriam, Gasqui Patrick, Lepetitcolin Elisabeth, Blanchard
Béatrice, Rousset Elodie, Sidi-Boumédine Karim, Jourdain Elsa. Coxiella burnetii circulation in a naturally infected
OIE Reference Laboratory Reports Activities, 2015
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Q fever - Elodie Rousset - france
dairy sheep flock: individual shedding, environmental contamination and strain diversity. ESCCAR International
Congress on Rickettsia and other Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015.
Jourdain Elsa, Duron Olivier, Séverine Barry, Daniel González-Acuña, Sidi-Boumedine Karim. Do genetic markers
routinely used to detect Coxiella burnetii in ticks cross-react with Coxiella-like bacteria? ESCCAR International
Congress on Rickettsia and other Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015.
Pellerin J-L, Alsaleh A., Rodolakis A., Rousset E., Dubreil L., Bruyas J-F., Fieni F.. Risk of transmission of Coxiella
burnetii during the caprine in-vitro produced embryo transfer. Xth International Congress of Veterinary Virology
ESVV2015 and 9th Annual Meeting of EPIZONE, Montpellier. 3 September. 2015.
Prigent Myriam, Rousset Elodie, Yang Elise, Thiery Richard, Sidi-Boumedine Karim. Validation study for using labon-chip technology for Coxiella burnetii multi-locus-vntr-analysis (mlva) typing. Application for studying genotypic
diversity of domestic ruminants’ strains in France. ESCCAR International Congress on Rickettsia and other
Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015.
Rousset Elodie, Dufour Philippe, Hans Aimeric, Gaudaire Delphine, Madeline Anthony, Lecollinet Sylvie, Leblond
Agnes, Thiéry Richard, Jourdain Elsa, Gasqui Patrick. Probabilistic model approach for evaluation of a multispecies serological method for Q fever. ESCCAR International Congress on Rickettsia and other Intracellular
bacteria, Lausanne, Switzerland, June 13-16, 2015.
Sidi-Boumedine Karim, Duquesne Véronique, Prigent Myriam, Yang Elise, Joulié Aurélien, Thiéry Richard, Rousset
Elodie. Impact of IS1111 insertion on the MLVA genotyping of C. burnetii. ESCCAR International Congress on
Rickettsia and other Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015.
Sidi-Boumedine Karim, Adam Gilbert, Angen Øysten, Aspán Anna, Bossers Alex, Roest Hendrik-Jan, Prigent
Myriam, Thiéry Richard, Rousset Elodie. Whole genome PCR scanning (WGPS) of C. burnetii strains from
ruminants. ESCCAR International Congress on Rickettsia and other Intracellular bacteria, Lausanne, Switzerland,
June 13-16, 2015.
c) National conferences: 7
Communication orale et acte de congrès
Joulié A., Laroucau K., Bailly X., Prigent M., Gasqui P., Le Petit Colin E., Blanchard B., Rousset E., Sidi-Boumedine
K., Jourdain E. Circulation de Coxiella burnetii dans un troupeau ovin lait naturellement infecté: dynamiques
d’excrétion, contaminations environnementales et diversité génotypique. Journées 3R, Paris, 2-3 Décembre,
2015.
Communication orale
Anastácio S, Pimenta L, Simões J, Alegria N, Rabiço A, Sidi-Boumedine K, da Silva GJ. Coxiella burnetii is present in
milk from dairy cattle herds in the Northwest Portugal. V Congresso Luso Brasileiro de Patologia Experimental XV
International Symposium on Experimental Techniques Joint Meeting. Coimbre, Portugal, 3-5 December 2015.
Joulié A., Sidi-Boumedine K., Rousset E., Leblond Agnès, Jourdain E. Epidémiologie moléculaire de Coxiella
burnetii, agent étiologique de la fièvre Q, chez les ruminants domestiques en France. Ecole Vétérinaire, Equipe
EPIDEC, Toulouse, 5 Octobre, 2015.
Joulié A., Sidi-Boumedine K., Rousset E., Leblond Agnès, Jourdain E. Diversité génotypique de Coxiella burnetii,
agent étiologique de la fièvre Q, chez les ruminants domestiques en France. Journée de clôture du dispositif
pilote, Paris, 1er Décembre, 2015.
Jourdain E, Carrié P., Barry S., Joulié A., Rousset E, Gasqui P., deCrémoux R., Sala C, Gache K., Calvas D. Détection
de Coxiella burnetii, agent étiologique de la fièvre Q, dans l’environnement. Journée de clôture du dispositif
pilote, Paris, 1er Décembre, 2015.
Rousset E., Dufour P., Sala C., Jourdain E. Analyse comparative des 3 kits ELISA pour la sérologie de la fièvre Q.
Journée de clôture du dispositif pilote, Paris, 1er Décembre, 2015.
Sidi-Boumedine K. The recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of
the Q fever pathogen, Coxiella burnetii. Journée Scientifique des Laboratoires, Anses, Maisons-Alfort, 18
Novembre, 2015.
6
OIE Reference Laboratory Reports Activities, 2015
Q fever - Elodie Rousset - france
Communications affichées
Gache Kristel et Groupe de suivi ESA 3-3 Thématique fièvre Q. Fièvre Q : dispositif pilote. Journée annuelle de la
plateforme d’Epidémiosurveillance Animale. Paris, 16 juin 2015.
Hosteing Soline et Groupe de suivi ESA 3-1 Thématique Avortements des ruminants. Déclaration des avortements
: surveillance événementielle de la brucellose. Journée annuelle de la plateforme d’Epidémiosurveillance Animale.
Paris, 16 juin 2015.
d) Other:
(Provide website address or link to appropriate information) 2
Rapport d’essai interlaboratoires d’aptitude : Sérologie fièvre Q sur sérum par ELISA, Session 2015. Rousset, E. et
P. Dufour (versions française et anglaise). Rapport Final, 13 avril 2015. 23 pages.
Rapport d’analyse quantitative des données issues de l’EILA sérologie fièvre Q sur serum, session 2015. Rousset,
E. et P. Dufour (versions française et anglaise). 18 mai 2015. 30 pages.
ToR 7: To provide scientific and technical training for personnel from OIE Member
Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member
Countries?
No
ToR 8: To maintain a system of quality assurance, biosafety and biosecurity
relevant for the pathogen and the disease concerned
15. Does your laboratory have a Quality Management System certified according to an International Standard?
Yes
Quality management system adopted
Certificate scan (PDF, JPG, PNG format)
ISO 17025
Attestation Cofrac 2015 2017.pdf
16. Is your laboratory accredited by an international accreditation body?
Yes
Test for which your laboratory is accredited
Accreditation body
ELISA sur sérum
COFRAC
17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned?
OIE Reference Laboratory Reports Activities, 2015
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Q fever - Elodie Rousset - france
Yes
(See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a)
ToR 9: To organise and participate in scientific meetings on behalf of the OIE
18. Did your laboratory organise scientific meetings on behalf of the OIE?
No
19. Did your laboratory participate in scientific meetings on behalf of the OIE?
No
ToR 10: To establish and maintain a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organise regular inter-laboratory
proficiency testing to ensure comparability of results
20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same
pathogen or disease?
Not applicable (Only OIE Reference Lab. designated for disease)
21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the
same pathogen or disease by organising or participating in proficiency tests?
Not applicable (Only OIE Reference Lab. designated for disease)
22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific
research projects for the diagnosis or control of the pathogen of interest?
Not applicable (Only OIE Reference Lab. designated for disease)
ToR 11: To organise inter-laboratory proficiency testing with laboratories other
than OIE Reference Laboratories for the same pathogens and diseases to ensure
equivalence of results
23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than
OIE Reference Laboratories for the same disease?
Yes
8
OIE Reference Laboratory Reports Activities, 2015
Q fever - Elodie Rousset - france
Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Purpose for inter-laboratory test comparisons1
Aptitude à obtenir un résultat sérologique
correct par ELISA sur sérums
No. participating
laboratories
87
Region(s) of participating OIE
Member Countries
Africa
Americas
Asia and Pacific
Europe
Middle East
ToR 12: To place expert consultants at the disposal of the OIE
24. Did your laboratory place expert consultants at the disposal of the OIE?
No
25. Additional comments regarding your report:
OIE Reference Laboratory Reports Activities, 2015
9