Laboratoire de Génie Enzymatique et de Microbiologie

Ecole Nationale d’Ingénieurs de Sfax
Laboratoire de recherche :
Laboratoire de Génie Enzymatique et de
Microbiologie
LR03ES08
Ecole Nationale d’Ingénieurs de Sfax
Route Soukra, Km 3.5, B.P 1173-3038
Sfax, TUNISIA
Responsable : Pr. Moncef NASRI
Email: [email protected]; [email protected]
Tel.: 216 96 501 698
Présentation Générale du LGEM
Le LGEM, créé en 2003, a pour mission de promouvoir une recherche pluridisciplinaire
qui vise l’acquisition de nouvelles connaissances scientifiques et de savoir-faire
technologiques dans les domaines du Génie Biologique, principalement la Technologie
Enzymatique et la Microbiologie Appliquée, disciplines scientifiques et techniques
importantes des Biotechnologies. Le laboratoire a pour mission également de promouvoir la
formation de scientifiques et d’ingénieurs dans le domaine du Génie Biologique.
Le laboratoire est dirigé par le Professeur Moncef NASRI et compte 30 personnes, dont
une dizaine d’enseignants-chercheurs qui encadrent des étudiants en thèse ou master.
Le laboratoire regroupe plusieurs équipes de compétences complémentaires dans les
domaines de la microbiologie, la biochimie, le Génie Enzymatique et le Génie Génétique.
Le LGEM est un ensemble dans lequel les collaborations horizontales sont très
importantes. Plusieurs chercheurs interviennent dans plusieurs thèmes.
Thèmes de Recherche
1/ Les protéases microbiennes
2/ Les protéases digestives de poisson
3/ Les Biopeptides
4/: Valorisation biotechnologique de produits et co-produits agro-alimentaires et marins
Projets :
Coopération Tuniso-Marocaine (2008-2010) : avec le Laboratoire de Chimie
Agroalimentaire et Substances Naturelles Végétales et Marines (LCASVM),
Département de Chimie, B.P. 5366 Maarif N 20100 Casablanca Maroc.
Mise en évidence de certaines activités biologiques dans les extraits et les hydrolysats
protéiques d’algues de Tunisie et du Maroc: Activités anti-oxydantes, anti-hypertensives et
antimicrobiennes.
CMCU : 08G908 avec le Laboratoire ECOLAG, Université Montpellier II, UMR 5119,
CNRS France.
Identification et caractérisation de protéases et de leurs propriétés (thermostabilité, résistance
au sel) chez Arthrobacter arilaitensis à partir des données de la séquence génomique.
ProBioGEM Lille France :
Hydrolysats peptidiques biologiquement actifs dérivés de protéines de co-produits de la
pêche. Préparation et caractérisation, fractionnement et identification de peptides actifs :
antioxidants, antihypertensifs, antimicrobiens, anti-thrombiques, etc.
Publications Internationales (2003/2010)
Bougatef Ali, Nedjar-Arroume Naima, Manni Laïla, Ravallec-Plé Rozenn, Barkia
Ahmed, Guillochon Didier and Nasri Moncef (2010)
Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of
sardinelle (Sardinella aurita) by-products proteins.
Food Chemistry, 118 : 559-565.
Bougatef Ali, Hajji Mohamed, Balti Rafik, Lassoued Imen, Triki-Ellouz Yosra and
Nasri Moncef (2009)
Antioxidant and free radical-scavenging activities of smooth hound (Mustelus mustelus)
muscle protein hydrolysates obtained by gastrointestinal proteases.
Food Chemistry, 114 : 1198-1205.
Haddar Anissa, Bougatef Ali, Agrebi Rym, Sellami-Kamoun Alya and Nasri Moncef
(2009)
A novel surfactant-stable alkaline serine-protease from a newly isolated Bacillus mojavensis
A21. Purification and characterization.
Process Biochemistry, 44 : 29-35.
Haddar Anissa, Agrebi Rym, Bougatef Ali, Hmidet Noomen, Sellami-Kamoun Alya and
Nasri Moncef (2009)
Two detergent stable alkaline serine-proteases from Bacillus mojavensis A21: Purification,
characterization and potential application as a laundry detergent.
Bioresource Technology 100 : 3366-3372.
Agrebi Rym, Haddar Anissa, Hmidet Noome, Jellouli Kemel, Manni Laïla and Nasri
Moncef (2009)
BSF1 fibrinolytic enzyme from a marine bacterium Bacillus subtilis A26: Purification,
biochemical and molecular characterization.
Process Biochemistry, 44 : 1252-1259.
Agrebi Rym, Haddar Anissa, Hajji Mohamed, Frikha Fakher, Manni Laila, Jellouli
Kemel and Nasri Moncef (2009)
Fibrinolytic enzymes from a newly isolated marine bacterium Bacillus subtilis A26 :
Characterization and statistical media optimization.
Canadian Journal of Microbiology, 55 : 1049-1061.
Fakhfakh Nahed, Kanoun Safia, Manni Laïla and Nasri Moncef (2009)
Production, biochemical and molecular characterization of a keratinolytic serine-protease
from a chicken feather-degrading Bacillus licheniformis RPk.
Canadian Journal of Microbiology, 55 : 427-436.
Noomen Hmidet, Nedra El-Hadj Ali, Anissa Haddar, Safia Kanoun, Sellami-Kamoun
Alya and Moncef Nasri (2009)
Alkaline proteases and thermostable α-amylase co-produced by Bacillus licheniformis NH1:
Characterization and potential application as detergent additive.
Biochemical Engineering Journal, 47 : 71-79.
Souissi Nabil, Bougatef Ali, Triki-Ellouz Yosra and Nasri Moncef (2009)
Production of lipase and biomass by Staphylococcus simulans grown on sardinelle (Sardinella
aurita) hydrolysates and peptone.
African Journal of Biotechnology, 8 : 451-457.
Jellouli Kemel, Bougatef Ali, Daassi Dalel, Balti Rafik, Barkia Ahmed and Nasri
Moncef (2009)
New alkaline trypsin from the intestine of Grey triggerfish (Balistes capriscus) with high
activity at low temperature: Purification and characterization.
Food Chemistry, 116 : 644-650.
Jellouli Kemel, Bougatef Ali, Manni Laïla, Agrebi Rym, Rayda Siala, Younes Islem and
Nasri Moncef (2009)
Molecular and biochemical characterization of an extracellular serine-protease from Vibrio
metschnikovii J1.
Journal of Industrial Microbiology and Biotechnology, 36 : 939-948.
Balti Rafik, Barkia Ahmed, Bougatef Ali, Ktari Naourez and Nasri Moncef (2009)
A heat-stable trypsin from the hepatopancreas of the cuttlefish (Sepia officinalis): Purification
and characterization.
Food Chemistry, 113 : 146-154.
Hajji Mohamed, Masmoudi Ons, Ellouz-Triki Yosra, Siala Rayda, Gharsallah Néji and
Nasri Moncef (2009)
Chemical composition and antioxidant and radical-scavenging activities of Periploca
laevigata root bark extracts.
Journal of the Science of Food and Agriculture, 89 : 897-905.
Syala Rayda, Sellami-Kamoun Alya, Hajji Mohamed, Abid Ines, Gharsallah Néji and
Nasri Moncef (2009)
Extracellular acid protease from Aspergillus niger I1: Purification and characterization.
African Journal of Biotechnology, 8 : 4592-4589.
Bougatef Ali, Ravallec Rozenn, Nedjar-Arroume Naima, Barkia Ahmed, Guillochon
Didier and Nasri Moncef (2009)
Evidence of in vivo satietogen effect and control of food intake of smooth hound (Mustelus
mustelus) muscle protein hydrolysate in rats.
Journal of Functional Foods (In press).
Barkia Ahmed, Bougatef Ali, Ben Khaled Hayet and Nasri Moncef (2009)
Antioxidant activities of sardinelle heads and/or viscera protein hydrolysates prepared by
enzymatic treatment.
Journal of Food Biochemistry (In press).
Manni Laila, Jellouli Kemel, Ghorbel-Bellaj Olfa, Agebi Rym, Haddar Anissa, SellamiKamoun Alya and Nasri Moncef (2009)
An oxidant- and solvent-stable protease produced by Bacillus cereus SV1: Application in the
deproteinization of Shrimp wastes and as a laundry detergent additive.
Applied Biochemistry and Biotechnology (In press).
Fakhfakh-Zouari Nahed, Hmidet Noomen, Haddar Anissa, Kanoun Safia and Nasri
Moncef (2009)
A novel serine-metallo keratinase from a newly isolated Bacillus pumilus A1 grown on
chicken feather meal: Biochemical and molecular characterization.
Applied Biochemistry and Biotechnology (In press).
Agrebi Rym, Hmidet Noomen, Hajji Mohamed, Haddar Anissa, Fakhfakh-Zouari Nahed
and Nasri Moncef (2009)
Fibrinolytic serine-protease from Bacillus amyloliquefaciens An6 grown on Mirabilis jalapa
tuber powders: Production, partial purification and biochemical characterization.
Applied Biochemistry and Biotechnolog (In press).
El-Hadj Ali Nedra, Hmidet Noomen, Bougatef Ali, Nasri Rima and Nasri Moncef
(2009)
A laundry detergent-stable alkaline trypsin from striped seabream (Lithognathus mormyrus)
viscera: Purification and characterization.
Journal of Agricultural and Food Chemistry (In press).
Bougatef Ali, Nedjar-Arroume Naima, Ravallec-Plé Rozenn, Leroy Yves, Guillochon
Didier, Barkia Ahmed and Nasri Moncef (2008)
Angiotensin I-converting enzyme (ACE) inhibitory activities of sardinelle (Sardinella aurita)
by-products protein hydrolysates obtained by treatment with microbial and visceral fish serine
proteases.
Food Chemistry, 111 : 350-356.
Hmidet Noomen, Bayoudh Ahmed, Berrin J.G, Kanoun Safia, Juge Nathalie and Nasri
Moncef (2008)
Purification and biochemical characterization of a novel α-amylase from Bacillus
licheniformis NH1. Cloning, nucleotide sequence and expression of amyN gene in
Escherichia coli.
Process Biochemistry, 43 : 499-510.
Manni Laila, Jellouli Kemel, Agrebi Rym, Bayoudh Ahmed and Nasri Moncef (2008)
Biochemical and molecular characterization of a novel calcium-dependent metalloprotease
from Bacillus cereus SV1.
Process Biochemistry, 43 : 522-530.
Sellami-Kamoun Alya, Haddar Anissa, El-Hadj Ali Nedra, Ghorbel-Frikha Basma,
Kanoun Safia and Nasri Moncef (2008)
Stability of thermostable alkaline protease from Bacillus licheniformis RP1 in commercial
solid laundry detergent formulations.
Microbiological Rechearch, 163 : 299-306.
Souissi Nabil, Triki-Ellouz Yosra, Bougatef Ali, Blibech Monia and Nasri Moncef
(2008)
Preparation and use of media for protease-producing bacterial strains based on by-products
from Cuttlefish (Sepia officinalis) and wastewaters from marine-products processing
factories.
Microbiological Research, 163 : 473-480.
Hajji Mohamed, Rebai Ahmed, Gharsallah Néji and Nasri Moncef (2008)
Optimization of alkaline protease production by Aspergillus clavatus ES1 in Mirabilis jalapa
tuber-powder using statistical experimental design.
Applied Microbiology and Biotechnology, 79 : 915-923
Jellouli Kemel, Bayoudh Ahmed, Manni Laila, Agrebi Rym and Nasri Moncef (2008)
Purification, biochemical and molecular characterisation of a metallo-protease from
Pseudomonas aeruginosa MN7 grown on shrimp wastes.
Applied Microbiology and Biotechnology, 79 : 989-999.
Bougatef Ali , Balti Rafik, Ben Zaied Saïda, Souissi Nabil and Nasri Moncef (2008)
Pepsinogen and pepsin from the stomach of smooth hound (Mustellus mustellus): Purification,
characterization and amino acid terminal sequences
Food Chemistry, 107 : 777-784.
Ben Khaled Hayet, Bougatef Ali, Balti Rafik, Triki-Ellouz Yosra, Souissi Nabil and
Nasri Moncef (2008)
Isolation and characterization of trypsin from sardinelle (Sardinella aurita) viscecra.
Journal of the Science of Food and Agriculture, 88 : 2654-2662.
Bougatef Ali, Souissi Nabil, Fakhfakh Nahad, Ellouz-Triki Yosra and Nasri Moncef
(2007)
Purification and characterization of trypsin from the viscera of Sardine (Sardina pilchardus)
Food Chemistry, 102 : 343-350
El-Hadj Ali Nedra, Agrebi Rym, Ghorbel-Frikha Basma, Sellami-Kamoun Alya,
Kanoun Safia and Nasri Moncef (2007)
Biochemical and molecular characterization of a detergent stable alkaline serine-protease
from a newly isolated Bacillus licheniformis NH1.
Enzyme and Microbial Technology, 40 : 515-523.
Hajji Mohamed, Kanoun Safia, Nasri Moncef and Gharsallah Néji (2007)
Purification and characterization of an alkaline serine protease produced by a new isolated
Aspergillus clavatus ES1.
Process Biochemistry, 42 : 791-797.
Souissi Nabil, Bougatef Ali, Triki-Ellouz Yosra and Nasri Moncef (2007)
Biochemical and functional properties of Sardinella (Sardinella aurita) by-product
hydrolysates.
Food Technology and Biotechnology, 45 : 187-194.
Ghorbel Sofiane, Souissi Nabil, Triki-Ellouz Yosra, Dufossé Laurent, Guérard Fabienne
and Nasri Moncef (2005). Preparation and testing of Sardinella protein hydrolysates as
nitrogen source for extracellular lipase production by Rhizopus oryzae
World Journal of Microbiology and Biotechnology, 21 : 33-38.
Ghorbel-Frikha Basma, Sellami-Kamoun Alya, Fakhfakh Nahed, Haddar Anissa, Manni
Laila and Nasri Moncef. (2005).
Production and purification of a calcium-dependent protease from Bacillus cereus BG1.
Journal of Industrial Microbiology and Biotechnology. 32 : 186-194.
Ammar Emna, Nasri Moncef, Medhioub Khaled. (2005).
Isolation of Enterobacteria able to degrade simple aromatic compounds from the wastewater
from olive oil extraction.
World Journal of Microbiology and Biotechnology, 21 : 253-259.
Chtourou Mohamed, Ammar Emna, Nasri Moncef and Medhioub K (2004).
Isolation of a yeast, Trichosporon cutaneum, able to use low molecular weight phenolic
compounds: application to olive mill waste water treatment.
Journal of Chemical Technology and Biotechnology, 79 : 869-878.
Ghorbel Basma, Sellami-Kammoun Alya and Nasri Moncef (2003).
Stability studies of protease from Bacillus cereus BG1.
Enzyme and Microbial Technology, 32 : 513-518.
Khannous Lamia, Souissi Nabil, Ghorbel Basma, Jarboui Raja, Kallel Monem, Nasri
Moncef and Gharsallah Néji (2003). Treatment of saline wastewaters from marine-products
processing factories by activated sludge reactor.
Environmental Technology, 24 : 1261-1268.
Triki-Ellouz Yosra, Ghorbel Basma, Souissi Nabil, Kammoun Sadok and Nasri Moncef
(2003). Biosynthesis of protease by Pseudomonas aeruginosa MN7 grown on fish substrate.
World Journal of Microbiology and Biotechnology, 19 : 41-45.
Hachicha Ridha, Rigane H, Ben Khodher M, Nasri Moncef and Medhioub Khaled
(2003). Effects of partial stone removal on the co-composting of olive-oil processing solid
residues with poultry manure and the quality of compost.
Environmental Technology, 24 : 59-67.
Dufossé Laurent, Richert L, Nasri Moncef and Guérard Fafienne (2003). Microbial
nitrogenous substrate from protein hydrolystae of non-tradable oysters, a marine equivalent to
yeast extract.
In « Marine Biotechnology : an overview of leading field », Colliec-Jouault S., Bergé J.P.,
Guézennec J., Fleurence J., Le Gal Y and Roy P. (eds), IFREMER, ISBN 2-84433-115-7,
169-175.
Kherouatou Nawel, Nasri Moncef and Attia Hamadi (2003)
A study of the Dromadery milk casein micelle and its changes during acidification.
Brazilian Journal of Food Technology, 6 : 237-244.
Publications : Accepté avec des révisions mineures ou majeures
Barkia Ahmed, Bougatef Ali, Nasri rima, Fetoui Emna, Balti Rafik, and Nasri Moncef
(2009)
Trypsin from the viscera of Bogue (Boops boops): Isolation and characterization.
Journal of the Science of Food and Agriculture.
Haddar Anissa, Sellami-Kamoun Alya, Fakhfakh-Zouari Nahed, Hmidet Noomen,
Fetoui Emna and Nasri Moncef (2009)
Alkaline proteases from Bacillus mojavensis A21: Compatibility with laundry detergents and
keratinolytic potential.
Biochemical Engineering Journal.
Fakhfakh-Zouari Nahed, Haddar Anissa, Frikha Fakher, Sellami-Kamoun Alya, Kanoun
Safia and Nasri Moncef (2009)
Degradation of poultry feathers and enzyme production by a newly isolated Bacillus pumilus
A1.
Process Biochemistry.
Manni Laïla, Ghorbel-Bellaj Olfa, Jellouli Kemel and Nasri Moncef (2009).
Isolation and characterization of chitin from shrimp wastes using enzymatic deproteinization
by Bacillus cereus SV1 protease.
Applied Biochemistry and Biotechnology.
Ouvrage
Recent Research Developments in Food By-products Technology and Biotechnology.
Published by Research Signpost 2008/2009.
Editor. Moncef NASRI
Articles dans des ouvrages
Ali Bougatef, Kemel Jellouli, Rafik Balti, Yosra Ellouz-Triki, Ahmed Barkia and
Moncef Nasri. 2008. Low molecular weight oxidant-stable protease from the intestine of
smooth hound (Mustelus mustelus). Purification and characterization. In Progress in Food
Chemistry (Editor: Ernst N. Koeffer). Nova Science Publishers
Nedra El Hadj-Ali, Noomen Hmidet, Nabil Souissi, Alya Sellami-Kamoun and Moncef
Nasri. 2008. The use of fish powders and gruel as growth substrate for the production of
alkaline serine proteases by Bacillus licheniformis NH1. In Food Sciences and Technology
(Editors: Lorenzo V. Greco and Marco N. Bruno). Nova Science Publishers
Eight Chapters in Recent Research Developments in Food By-products Technology and
Biotechnology. Published by Research Signpost 2008/2009.
Editor. Moncef NASRI.
Publications Nationales : Plus que 20 publications.
Communacations Nationales et Internationales : Plus que
200.
Brevets : 9
La liste des membres du laboratoire de recherche
Enseignants-chercheurs et personnels techniques
NASRI Moncef 01023471
Pr.
Temps
Diplôme en
consacré à
Etablissement
cours de
la
préparation
recherche
ENIS
100
SELLAMIKAMOUN
Alya
ELLOUZTRIKI Yosra
GHORBEL
Sofiane
KRIAA Habib
01083577
MC
FSS
100
04752222
M.A
ENIS
100
Habilitation
05254547
M.A
FSGafsa
70
Habilitation
01002595
M.A
FSS
10
Habilitation
KAMMOUN
Sadok
BARKIA
Ahmed
GHORBELFRIKHA
Basma
BAYOUDH
Ahmed
BOUGATEF
Ali
El-HADJ ALI
Nedra
MANNI Laila
02854666
M.A
ESH-CM
100
Habilitation
00869827
M.A
ESScTech Santé
100
Habilitation
05309692
MA
ISBS
100
Habilitation
01348992
MA
FSS
100
Habilitation
06149711
MA
ISBS
100
Habilitation
100
Habilitation
N946568
ENIS
20
Habilitation
HADDAR
Anissa
JELLOULI
Kemel
HAJJI Ayadi
05391951
AsTechnologue ISET
100
Thèse
Nom et
prénom
OUERDA
Hayet
GHAZALA
N°
C.I.N(1)
Grade(2)
Dr
05363198 AC
ISBS
100
Thèse
01068489
FLSH Kairouan
30
Thèse
ENIS
30
-
100
Dip Ingénieur
01112709
A
Pr principal
détaché
(Anglais)
08194034 Technicienne
ENIS
Imen
Etudiants : Thèse
Nom et prénom
N°
CIN(5)
Diplôme en
Nom et prénom
préparation Etablissement
de l’encadreur
(6)
AGREBI Rym
05392727
Thèse
ENIS
NASRI Moncef
SIALA ELLEUCH Raïda
05394529
Thèse
FSS
FAKHFAKH05394664
ZOUARI Nahed
HMIDET Noomen 05381480
Thèse
ENIS
Thèse
ENIS
HAJJI Mohamed
06022023
Thèse
FSS
SELLAMI Alya
VALLAEYS
Tatiana
NASRI Moncef
KANOUN Safia
NASRI Moncef
KANOUN Safia
NASRI Moncef
BALTI Rafik
07828098
Thèse
ENIS
BEN KHALED
Hayet
GHORBEL Olfa
08422311
Thèse
08170592
FETOUI Emna
KTARI Nawrez
Date de la
première
inscription
Octobre 2004
Octobre 2004
Octobre 2005
Octobre 2005
Octobre 2005
Octobre 2007
FSS
NASRI Moncef
ARROUME
Naïma
NASRI Moncef
Thèse
ENIS
NASRI Moncef
Mars 2007
08173359
Thèse
FSS
08173359
Thèse
ENIS
Janvier 2008
BARKIA Ahmed
Mars 2008
NASRI Moncef
NASRI Moncef Mai 2009
Etudiants : Mastère
Nom et prénom
N°
CIN(5)
Diplôme en
Nom et prénom
préparation Etablissement de l’encadreur
(6)
NASRI Rim
08809859
Mastère
ENIS
MASMOUDI Ons 08766421
Mastère
ENIS
MAALEJ Hana
08761337
Mastère
ENIS
LASSOUED Imen 08764676
Mastère
ENIS
BOUGATEF Ali
NASRI Moncef
HAJJI Mohamed
NASRI Moncef
HMIDET
Noomen
NASRI Moncef
Date de la
première
inscription
ISLAM Younes
06432245
Mastère/PFE ENIS
NASRI Moncef
DJERIDI Mourad
Mastère
ENIS
BARKIA Ahmed
SILA Assaad
Mastère
ENIS
BOUGATEF Ali
HAJJI Mohamed
NASRI Moncef
HAMDI
Mokhtar
SELLAMI
Kamoun Alya
SELLIMI Sabrine
08789527
Mastère
ENIS
CHTOUROU
Ihsène
08346728
Mastère
INSAT
SELLEMI Manel
08785922
Mastère
FSS