How to enrich dairy products with polyphenols and increase health

Factsheet
How to enrich
dairy products
with polyphenols
and increase
health benefits
Duration : 2011 – 2016
Highlights
• Thisprojectispartofaninitiativetoidentifythenutritionalsynergiesand
protectiveeffectsofdairyproductsonnutrientsinotherfoodgroups.
• Inregardstoobjective1,thisstudyisthefirstofitskindtodemonstratethatthe
presenceofcalciumhasalargeinfluenceontheinteractionbetweenEGCG/
proteinβlgandEGCG/proteinalphas1casein.Inthecaseofbeta-lg,the
presenceofEGCGisrequiredinordertohaveanincreasedparticlesizein
accordancewithincreasedcalciumconcentrations.
• Inobjective2,thestudydemonstratedthatmilkhasthestrongestfixationability
forpolyphenolsfromgreenteawitharatethatisslightlyhigherthan80%,while
theaveragefixationrateofyogurtis75%forpolyphenols.However,cheese
matriceshaveagreaterprotectiveeffectonpolyphenolsduringdigestion.
• Accordingtotheseresults,calciumsupplementationisimportantformilkthatis
enrichedwithstrongconcentrationsofextractsrichinpolyphenols.Inaddition,it
appearsthatpolyphenolsinfluencethedigestionofdairyproductsbydecreasing
theratesofliberationofpeptidesandfattyacids.
• Theresultsconfirmfortheveryfirsttimethatdairymatriceshaveaprotective
effectontheantioxidantactivityofpolyphenoliccompoundsduringdigestion.
However,polyphenolsincranberriesaremoresensitivetothegastro-intestinal
environmentthanpolyphenolsingreentea.
• Concerningobjective3,theresultssupportthatβ-lghasbeneficialeffectson
glycemiccontrolbutsuggestthattheconsumptionofEGCGandcalciumdonot
increasethiseffect.
*EGCG:epigallocatechin-3-gallate
Objectives
Inordertostudythenutritionalsynergybetweenmilkcomponentsandpolyphenols
containedinteaandcranberryjuice,thisstudyfocusesonthreemainobjectives:
• Tocharacterizetheinteractionsbetweenmilkcomponentsandisolatedphenolic
compoundsinteaandcranberryjuice;
• Tomeasure,duringdigestion,theeffectofmilkcomponentsandthreedairy
matrices(milk,yogurt,cheese)ondegradationkineticsandantioxidantactivityof
phenoliccompounds;
• Tomeasure,postdigestion,theeffectofthepresenceofmilkcomponentsonthe
physiologicalactivityofpolyphenols.
Results and potential benefits
Research on these three components has primarily
helped to advance knowledge in the dairy sector since
manyoftheresultshavebeendemonstratedforthevery
first time. In addition, the knowledge and results
obtained from this project will contribute to the
developmentofnewpolyphenol-enricheddairyproducts
whiledemonstratingthebeneficialeffectoftheaddition
of polyphenols on health or during digestion. With the
knowledge acquired during this project on the
interactionsbetweenpolyphenols/calcium/dairyproteins
(alpha 1 casein and β lg), new dairy ingredients may be
developed(proteinaggregatesrichinECGCandcalcium).
Depending on the interests of dairy processors, these
results may be used as part of a knowledge transfer
project conducted in-plant. The results concerning the
effect of polyphenols from grapes (commercial extracts)
onrennet-inducedcoagulationcouldalsobevalidatedinplantbeforehand.Inaddition,resultsdemonstratethatit
is possible to preserve the antioxidant activity of
Forum Technologique 2016
polyphenols according to the type of dairy matrix and
the manner in which they are incorporated. Lastly,
consumersbenefitfromknowingthisinformationabout
the liberation of polyphenols during the digestion of
milk matrices, as well as about the protection and
liberationofpolyphenols,astheresearchdemonstrates
the real impacts and benefits of milk components
(beneficial effect on postprandial glycemia). The
potential benefits of the research results are: 1)
Increased revenues due to an increased demand for
dairyproducts:Reinforcementofthe“healthy”imageof
milk. In additional to its high nutritional density, milk
provides “protection” to nutrients from other food
groups (nutritional synergies); 2) Increased product
quality: Effect of milk proteins and calcium on the
protection and liberation of polyphenols during
gastrointestinal digestion; and 3) The ability to better
respondtoconsumers’concerns.
Professionals trained
Contact persons
Project managers :
Laurent Bazinet
Centre de recherche STELA/INAF,
Local 1403, Département de
Sciences des Aliments (ALN),
2425 rue de l’Agriculture,
Université Laval, Québec (QC)
G1V 0A6
(418) 656-2131 # 7445
[email protected]
www.laurentbazinet.fsaa.ulaval.ca
Michel Britten
Agriculture and AgriFood Canada,
3600 boul. Casavant Ouest, StHyacinthe (QC) J2S 8E3
(450) 768-3235
[email protected]
MartineLussier:Master'sdegree(incomplete)
Expertiseacquired:
• Methodsofanalyzingpolyphenol-proteininteractions
• Methodsofanalyzingantioxidantproperties(DPPH,ORAC)
• Fractioningtechniquesandcharacterizationofproteinsfoundindairy
products
• Dosagetechniques
• Operationofastaticdigestionsimulator
ValérieCarnovale:PhD(completed)
Expertiseacquired:
• Methodsofanalyzingpolyphenol/calcium/milkproteininteractions
• Effectsofdairyproductsandtheirinteractionsonphysiologicalresponses
• Physicalchemistryofproteins
• Physiologyanddigestionofdairyproducts
For further information
•Lussier,M.;Britten,M.;Couillard,C.;
•
Bazinet,L.Interactionentrelespolyphénols
d'unextraitdethévertetlesmatrices
laitières:impactsurlesactivitésantiradicalaireetanti-oxydante.Poster
presentedduringthefirstresearchdayat
theFacultyofAgricultureandAgri-Food
Sciences,“Mieuxnourrirlemonde.”
QuebecCity(Qc),November28,2012.
•
•Lussier,M.;Britten,M.;Couillard,C.;
Bazinet,L.Interactionentrelespolyphénols
d'unextraitdethévertetlesdifférentes
formedematriceslaitières.Poster
presentedduringtheSTELASymposium
“InnovationfortheFutureoftheDairy
Sector”andtheFIL-IDF“DairyOutlook
Seminar,”Montreal(Qc),May13‒16,2013.
•Lussier,M.;Britten,M.;Couillard,C.;
Bazinet,L.Interactionentreles
polyphénolsd’unextraitdethévertetles
matriceslaitières.Posterpresentedduring
theNovalaitTechnologicalForum
“Carrefourdescompétences,savoirset
savoir-fairelaitiers,”Drummondville(Qc),
May28,2014.
•Carnovale,V.;Britten,M.;Couillard,C.;
Bazinet,L.Effetdecomplexes
épigallocatéchine-3-gallateetlaβLactoglobulineformédansunultrafiltrat
delaitsimuléavecetsanscalciumsurla
réponsemétaboliquechezlasouris.
Posterpresentedduringthe2015STELA
Symposium“ChallengesandOpportunities
fortheDairySector.”PosterNo.27.
QuebecCity(Qc),June1‒2,2015.
•Carnovale,V.;Britten,M.;Couillard,C.;
Bazinet,L.Étudeetimpactsdes
• •Carnovale,V.;Labaeye,C.;Britten,M.;
interactionsentrel’épigallocatéchine
Couillard,C.;Bazinet,L.Impactducalcium
gallate,labêta-lactoglobulineetlecalcium
surlesinteractionsentre
danslaphaseminéraledulait.Poster
épigallocatéchine-3-gallateetlaβpresentedduringtheNovalait
Lactoglobuline.Posterpresentedduring
TechnologicalForum“Carrefourdes
the2015STELASymposium“Challenges
compétences,savoirsetsavoir-faire
andOpportunitiesfortheDairySector.”
laitiers,”Drummondville(Qc),May28,
PosterNo.28.QuebecCity(Qc),June1‒2,
2014.
2015.
Financial contributions
Partnershipforinnovationindairyproductionanddairyprocessing
(EPI2011-2017):
-FondsderechercheduQuébec–Natureettechnologies
-Ministèredel’Agriculture,desPêcheriesetdel’AlimentationduQuébec
-Novalait
Totalbudget:$250000
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