ASSELINE Ulysse-CURRICULUM VITAE Asseline ulysse Education

ASSELINE Ulysse-CURRICULUM VITAE
Asseline ulysse
Directrice de Recherches
Professional address : Centre de Biophysique Moléculaire UPR- 4301- CNRS.
Rue Charles Sadron - 45071 - Orléans Cedex 2
Tel : (02) 38 25 55 97
E-mail: ulysse.asseline@cnrs-orleans
Education
1983
Doctorat d’Etat ès-Sciences Physiques. Université d’Orléans. Titre : Synthèse et
études physicochimiques d’oligonucléotides modifies et non modifiés.
Directeur de thèse : Pr C. Hélène. Boursière de la Ligue Nationale Française Contre
le Cancer.
1980 Doctorat de 3e Cycle. Université d’Orléans. Titre: Contribution à l’étude des dérivés
monosubstitués des acides du phosphore tétracoordiné : Application à la synthèse des
nucléotides.
Directeur de thèse : Dr P. Chabrier. Allocataire DGRST.
Academic positions and stays
1985
CNRS researcher (CR1) - Centre de Biophysique Moléculaire UPR - 4301 -CNRS
Orléans. Team: Oligonucleotides
01-02-1991-31-01-1992. Laboratory of Molecular Biology- Medical Research Council
Cambridge (UK). Contribution to Tat-TAR interaction studies of
VIH 1 (Team: Dr M. Gait).
1996-present. Directrice de Recherches CNRS (Senior CNRS researcher). Centre de
Biophysique Moléculaire UPR-4301-CNRS Orléans
1997-2011.
Team leader. Oligonucléotides Modifiés: chimie et applications
2011-2016. Team: Optical sensors and oligonucleotidic probes for bioanalyses and
imaging (leaders: Dr. S. Petoud then Pr J. Hamacek).
Research activities
Research activities focus on the development of novel oligonucleotides with the purpose to
obtain tools for basic research, in vitro diagnostics, bio-imaging, as well as potential
therapeutic agents.
Thus, depending of the intended applications, the oligonucleotides should possess several of
the following properties: to be able to form specific and stable complexes with their targets
and either to induce irreversible modifications of the targets (by cleavage or cross-linking
reactions) or to produce a modified signal, in the presence of the target, that can be used as
a proof of hybridization. Furthermore, for the applications in cultured cells and in vivo the
oligonucleotides must be resistant to nucleases and capable of reaching their targets inside
the cells.
In order to provide the oligonucleotides with the required properties for those applications,
we chemically modify the different structural units of their backbones (nucleic bases,
internucleotidic linkages, sugar moieties). These modified oligonucleotides are covalently
attached to various ligands (intercalating agents, cleavage and cross-linking reagents,
peptides, lipophilic compounds, topoisomerase inhibitors, visible and NIR labels ….). The
development of conjugates with optimized properties depends on linkage parameters
between both entities (positions of attachment on the oligonucleotides and the ligands,
length and nature of the linkers). The chemical syntheses of these new oligonucleotide
series require the development of new reagents and synthetic methods.
These interdisciplinary programs are developed in the framework of collaborative work with
biochemists and biologists at CBM as well as with other academic laboratories and private
companies. (Financial supports: CEE-BioMed, ANRS, CNRS, ANR, Region Centre,
Département du Loiret and private companies).
Scientific production
100 publications (including 3 reviews and 11 book chapters) + 2 patents
H-index (Web of Science) 29
Teaching
1993-2013
Cours de Chimie des oligonucléotides en DEA puis Master 2 chimie.
DEA de chimie et physicochimie des composés d’intérêt biologique (Université
d’Orléans, Paris V, Paris XI) de 1993 à 2000.
DEA Conception, synthèse, analyse et structure des composés d’intérêt
biologique puis en Master 2 COSAMIB (Université d'Orléans) de 2000 à 2013.
2006-2008
Cours de chimie des oligonucléotides en Master 1 de Biochimie (Université
d’Orléans).
1980-1982 Travaux pratiques de chimie organique. IUT de Chimie Orléans