Frédéric Lopez Groupe protéomique, CRCT, Toulouse, France

Frédéric Lopez
Groupe protéomique, CRCT, Toulouse, France
Couplage BIA-MS pour l’identification d’un ITAF (IRES
transacting factor) du VEGF-D
VEGF-D promotes tumor metastases by inducing lymphangiogenesis and lymphatic
vasculature dilatation. In this study, we demonstrated the molecular mechanisms involved
in VEGF-D-induced lymphatic dilatation. We identified a stress-induced translational
regulation of VEGF-D expression through an IRES activation under increased temperature
conditions. We showed that VEGF-D IRES activity was dependent of prostaglandin
pathway as non steroidal anti-inflammatory drugs are able to abolish the IRES-promoted
VEGF-D protein synthesis, and lymphatic vessels dilatation. Using plasmon surface
resonance on biotinylated VEGF-D mRNA coupled to mass spectrometry, we identified
the IRES transacting factor (ITAF), the Nucleolin, that allows the recruitment of the
ribosome to promote VEGF-D translational initiation thanks to a relocalization into the
cytoplasm.
ProteoPACA 2015 – 17 Juin 2015, Marseille – FRANCE