LUCIA To Decipher Metal Homeostasis And Toxicity In Plants

LUCIA To Decipher Metal Homeostasis And Toxicity
In Plants
Marie-Pierre Isaure1, Stéphanie Huguet1, Louis Grillet2, Stéphane Mari2,
Nicolas Trcera3
1
Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, Institut des sciences
analytiques et de physico-chimie pour l’environnement et les matériaux (LCABIE/IPREM-UMR 5254),
Université de Pau et des Pays de l'Adour and CNRS, Hélioparc, 2 Av. Pierre Angot, 64053 Pau Cedex
9, France.
2
Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des
Plantes, Centre National de la Recherche Scientifique (UMR5004), Institut National de la Recherche
Agronomique, Université Montpellier II, Ecole Nationale Supérieure d’Agronomie, 34060
Montpellier Cedex 2, France.
3
SOLEIL Synchrotron LUCIA Beamline, BP48, 91192 Gif sur Yvette, France.
ABSTRACT
Some metals such as iron (Fe) are essential to plants whilst others such as cadmium (Cd)
are toxic even at low concentration. The identification of the chemical forms of these metals
(speciation) and their localization at the tissue and cell level are crucial to understand their
trafficking and storage and finally the ability for the plant to cope with their potential toxicity or
requirement. LUCIA beamline offers the possibility to access the metal distribution thanks to
micro X-ray fluorescence with a micrometer sized lateral resolution and to investigate the
metal speciation using micro X-ray Absorption Near Edge Structure. Importantly, the
beamline is designed for measurements in cryogenic conditions, which is necessary to limit
metal redistribution and speciation change in biologic samples. Here, we present two
examples of our work performed on Lucia during the last years. The first one deals with Fe
homeostasis and the discovery of an important pool of iron in plant cell nucleus (1) as well as
the Fe species in plant embryos (2). The second example is dedicated to the study of Cd
sequestration in Anthyllis vulneraria used as a pioneer plant in the phytostabilization of
mining sites (3).
REFERENCES
1. H. Roschzttardtz, L. Grillet, M.P. Isaure, G. Conejero, R. Ortega, C. Curie, and S. Mari, J. Biol. Chem. 286, 27863-27866 (2011).
2. L. Grillet, L. Ouerdane, P. Flis, M. Thi Thanh Hoang, M. P. Isaure, R. Lobinski, C. Curie, and S. Mari, J. Biol. Chem. 289, 2515-2525
(2014).
3. S. Huguet, S. Soussou, J. C. Cleyet-Marel, N. Trcera, M. P. Isaure, ‘Rhizostabilization of a mine tailing highly contaminated:
preliminary study of Cd localization and speciation in Anthyllis vulneraria in ‘Proceedings of the 16th International Conference on
Heavy Metals in the Environment’, edited by N. Pirrone, Roma, Italy, 2013, n°19008.