EATLAS DATA CATALOGUE (AIMS)
Transcription
EATLAS DATA CATALOGUE (AIMS)
EATLAS DATA CATALOGUE (AIMS) Acute toxicity of photosystem II herbicides to tropical seagrass 2013. (NERP TE 4.2, AIMS and JCU) Métadonnées | Métadonnées (XML) | Titre Acute toxicity of photosystem II herbicides to tropical seagrass 2013. (NERP TE 4.2, AIMS and JCU) Date 2015-09-08 Type de date Publication Résumé This dataset shows the measured response of the photosystems of seagrasses to herbicides in experiments conducted in 2012-2013. The data is provided as a multi-sheet spreadsheet. The aim of this study was to apply standard ecotoxicology protocols to quantify the concentrations of four priority PSII herbicides that inhibit photochemistry by 10, 20 and 50% (IC10, IC20 and IC50) over 72 hrs in two common seagrass species from the GBR lagoon. This data will enable improved assessment of the risks posed by PSII herbicides to tropical seagrass for both regulatory purposes and for comparison with other taxa. Methods: Four seagrass species (H. uninervis, C. rotundata, T. hemprichii and Z. muelleri) were used in preliminary studies to determine the time taken for PSII herbicides to affect photosynthesis, while more detailed ecotoxicology studies were undertaken with two of these species (H. uninervis and Z. muelleri). H. uninervis, C. rotundata and T. hemprichii were collected from intertidal seagrass meadows (<1 m) from Cockle Bay, Magnetic Island (19°10.88’ S, 146°50.63’ E) while Z. muelleri was collected from Pelican Banks, Gladstone, Australia (23°46.005’ S, 151°18.052’ E). Halodule uninervis, Cymodocea rotundata Ascherson (Cymodoceaceae) and Thalassia hemprichii Ascherson (Hydrocharitaceae) are tropical seagrass species widely distributed throughout the Indo-West Pacific while Zostera muelleri Irmisch ex Ascherson (Zosteraceae), (syn Zostera capricorni) is a tropical to temperate species found in Australia and New Zealand. Potted seagrasses were exposed to dissolved herbicides in static-replacement seawater (24 h water changes). All experiments were conducted under 273 ± 17 µmol photons m-2 s-1 (12h light:dark photoperiods, Aqua Illumination LED). This light intensity was chosen as the median daily irradiance at the Magnetic Island collection site. The glass aquaria were placed into water baths and maintained at 25.8 ± 0.3°C (range), equivalent to the annual average temperature in the GBR. Herbicide concentrations ranged from 0.1 to 300 µg herbicide l-1 depending on the potency of the herbicide. Duplicate tanks were used for each herbicide concentration and each tank contained individually potted seagrass plant of each species. eAtlas Data Catalogue - AIMS | 2017-02-23 | 1 / 3 Inhibition of photosynthesis was measured after 72 h exposure using pulse amplitude modulation (PAM) fluorometry. Two parameters were measured (effective quantum yield, deltaF/F’m and maximum quantum yield, Fv/Fm). The inhibition of photosynthetic yields relative to controls were plotted as dose-response curves by fitting inhibition data with measured concentrations using a 4 parameter logistic model (SigmaPlot 11). The herbicide inhibition concentrations (ICxx) that inhibited deltaF/F’m and Fv/Fm by 10, 20 and 50% (IC10, IC20 and IC50, respectively) were determined from each curve. Initially a series of pilot studies were performed to measure the time it takes for the four PSII herbicides to illicit 90% steady state (maximum) inhibition of effective quantum yield (deltaF/ F’m) in Z. muelleri at single herbicide concentrations. These findings were used to ensure that the exposure duration of later dose-response curves was sufficient. The nominal herbicide concentrations used were 10 µg l-1 Diuron, 50 µg l-1 Atrazine, 10 µg l-1 Hexazinone and 400 µg l-1 Tebuthiuron. This data can be found in the “Kinetics” worksheet. We also exposed all four species of seagrass to 10 µg l-1 Diuron to examine the consistency of response times between species. Inhibition of deltaF/F’m by the herbicides compared with carrier controls were conducted at multiple times up to 24 h. This data can be found in the “4 seagrasses at Diuron 10ug_L” worksheet. Further information can be found in this publication: Flores F, Collier CJ, Mercurio P, Negri AP (2013) Phytotoxicity of Four Photosystem II Herbicides to Tropical Seagrasses. PLoS ONE 8(9): e75798. doi: 10.1371/ journal.pone.0075798 Format: Acute_seagrass_toxicity_data_(Flores_et_al_2013).xlsx is an Excel SpreadSheet containing multiple worksheets. Each sheet has a description such as this: - Tank Rep: tank replicate (two tanks A and B) - deltaF/F’m: effective quantum (light adapted) yield measured by a Pulse Amplitude Modulated (PAM) fluorometer - Fv/Fm: maximum quantum (dark adapted) yield measured by a Pulse Amplitude Modulated (PAM) fluorometer - Solvent cont: no herbicide but contains less than 0.03% v/v ethanol carrier as per the treatments - Halodule uninervis: a seagrass species - Zostera muelleri: a seagrass species - Thalassia hemprichii: a seagrass species - Halophila ovalis: a seagrass species - 72 h: the duration of the herbicide exposure in some experiments - Time (hr): duration of exposure in hours Langue des métadonnées eng Jeu de caractère Utf8 Type de la ressource Jeu de données Ressource en ligne Adresse Internet http://eatlas.org.au/data/uuid/e1f65eed-5d30-413c-b70c-af85d776d25b Protocole WWW:LINK-1.0-http--metadata-URL Adresse Internet http://eatlas.org.au/nerp-te/gbr-aims-pesticide-effects-4-2 Protocole WWW:LINK-1.0-http--related Adresse Internet http://eatlas.org.au/pydio/data/public/nerp-te-4-2_aims_herbicide-toxicity-to-seagrass_2013zip.php Protocole WWW:LINK-1.0-http--downloaddata Adresse Internet http://dx.doi.org/10.1371/journal.pone.0075798 Protocole WWW:LINK-1.0-http--link Contact pour la ressource eAtlas Data Catalogue - AIMS | 2017-02-23 | 2 / 3 Nom de la personne Negri, Andrew, Dr Nom de l'organisation Australian Institute of Marine Science (AIMS) Rôle Point de contact Catégorie ISO Biologie, faune et flore Mot Clé Mot Clé marine Mot Clé seagrass Mot Clé Diuron Mot Clé Atrazine Mot Clé Hexazinone Mot Clé Tebuthiuron Mot Clé herbicide Type de mot clé Thème Etendue Description Australian Institute of Marine Science (AIMS) Description Pelican Banks, Gladstone, Australia Collection site for: Z. muelleri Description Cockle Bay, Magnetic Island, Australia Collection site for: H. uninervis, C. rotundata and T. hemprichii Emprise géographique Ouest 147.05599 Est 147.05599 Sud -19.27229 Nord -19.27229 Ouest 151.3009 Est 151.3009 Sud -23.76675 Nord -23.76675 Ouest 146.8438 Est 146.8438 Sud -19.18133 Nord -19.18133 Identifiant de la fiche e1f65eed-5d30-413c-b70c-af85d776d25b Langue des métadonnées eng Jeu de caractère Utf8 Contact Nom de la personne eAtlas Data Manager Nom de l'organisation Australian Institute of Marine Science (AIMS) Rôle metadataContact Date des métadonnées 2015-09-17T19:44:13 eAtlas Data Catalogue - AIMS | 2017-02-23 | 3 / 3