Research group: Capillary Electrokinetic

Research group: Capillary Electrokinetic Separation Methods
1. Members
Group leader: Pierre GAREIL, Professor ([email protected])
Anne VARENNE, Assistant-professor ([email protected])
Nathalie DELAUNAY, Research scientist CNRS ([email protected])
Fanny D'ORLYE, Ph-D student ([email protected])
Mélanie LAGARRIGUE, Ph-D student ([email protected])
Maxime LEVERT, Ph-D student ([email protected])
Mériem MOKADDEM, Ph-D student ([email protected])
Stéphane GOYARD, Ph-D student
2. Aims of research
The main aims of the team are the development of (i) separation methods, namely capillary
zone electrophoresis (CZE), frontal analysis capillary electrophoresis (FACE), capillary
isoelectrofocusing (CIEF), isotachophoresis (CITP), and electrochromatography (CEC), and
(ii) the application of hyphenation of electrokinetic separation methods to mass spectrometry
(CE-MS), as a way to improve analysis selectivity and sensitivity. The work is also focused
on the characterization of non-covalent interactions in solution and at liquid-solid interfaces
and the determination of physicochemical properties of some solutes and media.
3. Research topics
- electrokinetic separations in hydro-organic or totally organic media and in the
presence of ionic liquids. Organic media enable to separate positional isomers such as fucose
monosulfates by modulating ion pairing and solvation phenomena, or amphetamines by
modulating ion pairing effects and ionization degree. Applications to the analysis of
hydrophobic proteins by isoelectrofocusing in the presence of glycerol were also developed.
- electrokinetic preconcentration methods, for the analysis of compounds at trace level.
Involving field-amplified based-stacking effect and isotachophoretic stacking effect (alone or
coupling both mechanisms), we succeeded in analyzing trace cationic impurities in ionic
liquids, chemical warfare agents in soils and metal cations in heat exchanger fluids of nuclear
power plants.
- methodology of the coupling of mass spectrometry (MS) to capillary electrophoresis
(CE). Several approaches were developed to maintain the electrical contact for electrokinetic
separation, in order to prevent from electrolyte depletion at the outlet end of a bare fusedsilica capillary for the analysis in negative polarity mode of polyanionic compounds having an
absolute-value of the electrophoretic mobility superior to the electroosmotic one. These
methodological developments allow to exploit fully MS advantages, such as its ability to
specifically fragment isomers which co-migrate in electrophoresis, which illustrates the high
complementarity of separation selectivity provided by CE and of detection selectivity provide
by MS. CE-MS thus associates high separation power, high sensitivity, and important mass
and structural information and is nowadays a key method to resolve complex mixtures such as
the ones encountered in metabolomic.
- interaction characterization. Experimental approaches and data treatment were developed
to characterize non-covalent interactions. As examples, active pharmaceutical ingredient
incorporation in liposomes (interaction constant and stœchiometry), protein-polysaccharide
complexes, surfactant aggregation threshold and inclusion complexation, such as that of ionic
liquid cations in cyclodextrins, were studied.
- characterization of physico-chemical properties. As an example, an on-line and
automated protocol which requires very low sample volumes was developed to determine
viscosity, conductivity and UV absorbance of ionic liquids. The determination of zeta
potential and charge density of nanoparticules or the measurement of size distribution of
synthetic and natural polyelectrolytes or nanoparticules constitute other examples.
Cuurent application fields:
- chiral molecules (enantioseparation)
- positively and negatively charged
- amphiphilic compounds, surfactants
- ionic liquids
nanoparticules (10-500 nm)
- synthetic polyelectrolytes
- trace inorganic and organic cations
- natural polyelectrolytes: proteins,
- cell metabolites
polysaccharides, DNA, humic acids
- warfare agents
4. Some innovative results
5. News and scientific production (2004 to 2006)
5.1 Academic collaborations
- ESPCI, Lab. of environment and analytical chemistry, Paris, France
- Bichat Hospital, Lab. of ecology and evolution of microorganisms, Paris, France
- Institute of Physico-Chemical Biology, Lab. of molecular physico-chemistry of
biological membranes, Paris, France
- University of Paris VI Pierre et Marie Curie, Lab. of ionic liquids and charged
interfaces, Paris, France
- University of Evry Val d’Essonne, Lab. of analysis and environment, Evry, France
- ENSI Caen, France
- University of Rouen, France
- National Institute of Applied Sciences and Technology, Tunis, Tunisia
- University of Genova, Lab. of pharmaceutical analytical chemistry, Switzerland
- University of Liège, Belgium
- Czech Academy of Sciences, Brno, Czech Republic
5.2 Industrial partnerships
- Commissariat à l'Energie Atomique (CEA) (DAM), Valduc, France
- Direction Générale de l'Armement (DGA), Research Center of Le Bouchet, France
- Electricité De France (EDF), Research Center of Les Renardières, Moret-sur-Loing,
France
- Guerbet, Roissy, France
- Innate Pharma, Marseille, France
- Institut Français du Pétrole (IFP), Solaize, France
- Laboratoire Français de Fractionnement et de Biotechnologies (LFFB), Les Ulis,
France
- Sébia, Evry, France
- Sanofi-Aventis, Research Center of Paris, France
5.3 Specialized courses
- Solution chemistry, Physico-chemistry of new media, Ionic Liquids, Separation
methods (chromatography, capillary electrophoresis), Coupling to mass spectrometry,
Tracability and analytical methods, Chemistry, Analysis and Environment
- continuing education "Electrophorèse capillaire et techniques apparentées"
(Language: French)
- June 4-8, 2007
- October 8-12, 2007
5.4 Seminars and symposia
- March 20-22, 2007: SEP 07 (www.sep07.com), Techniques Séparatives et
Couplages, Grenoble, France
- Discussions of Club Ile de France from AfSep (Association francophone des
Sciences Séparatives, www.afsep.com): 3 seminars per year,
Next seminar: December 14, 2006, Petites molécules d'intérêt biologique /
bioanalyse, ESPCI, Paris
- August 28-30, 2006: ITP 2006, 15th International Symposium on Capillary
Electroseparation Techniques, Paris, France
- September 26-29, 2005: SCBA 2005, 1er Symposium de Chimie et Biologie
Analytiques, Montpellier, France
- October 4-8, 2004: ISC 04, 25th International Symposium on Chromatography, Paris,
France
- June 15-19, 2003: HPLC 03, 27th International Symposium on High Performance
Liquid Phase / Separations and Related Techniques, Nice, France
- May 13-15, 2003: SEP 03, Techniques Séparatives et Couplages, Lyon, France
5.5 Publications with lecture committee
Determination
of
Aqueous
Inclusion
Complexation
Constants
of
Ionic
Liquid
Alkyl(methyl)methylimidazolium cations and neutral Cyclodextrins, Y. FRANÇOIS, A.
VARENNE, J. SIRIEIX, P. GAREIL, J. Sep. Sci., accepted
Evaluation of Chiral Ionic Liquids as New Chiral Selectors for Capillary Electrophoresis, Y.
FRANÇOIS, A. VARENNE, E. JUILLERAT, D. VILLEMIN, P. GAREIL, J. Chromatogr. A, under
revision
CE-MS-MS for the identification of chemical warfare agent degradation products, M.
LAGARRIGUE, A. BOSSE, A. BEGOS, A. VARENNE, P. GAREIL, B. BELLIER, LC-GC Eur.,
submitted, invited paper.
Separation and identification of isomeric acidic degradation products of organophosphorus
chemical warfare agents by capillary electrophoresis-ion trap mass spectrometry, M.
LAGARRIGUE, A. BOSSEE, A. BEGOS, A. VARENNE, P. GAREIL, B. BELLIER, J. Chromatogr.
A, accepted
Determination of Trace Cationic Impurities in Butylmethylimidazolium-based Ionic Liquids:
from Transient to Comprehensive Single-capillary Counter-flow Isotachophoresis-Zone
Electrophoresis, M. URBANEK, A. VARENNE, P. GEBAUER, L. KRIVANKOVA, P. GAREIL,
Electrophoresis, 2006, accepted, special issue, invited paper.
Nonaqueous Capillary Electrophoretic Behavior of 2-arylpropionic Acids in the Presence of an
Achiral Ionic Liquid: a Chemometric Approach, Y. FRANÇOIS, A. VARENNE, E. JUILLERAT,
A.-C. SERVAIS, P. CHIAP, P. GAREIL, J. Chromatogr. A, accepted
Determination of the Binding Parameters for Antithrombin-Heparin Fragments Systems by
Affinity and Frontal Analysis Continuous Capillary Electrophoresis, T. LE SAUX, A.
VARENNE, F. PERREAU, L. SIRET, S. DUTEIL, L. DUHAU, P. GAREIL, J. Chromatogr. A, 2006
(1132) 289-296
New integrated measurement protocol using capillary electrophoresis instrumentation for the
determination of viscosity, conductivity and absorbance of ionic liquid-molecular solvent
mixtures, Y. FRANÇOIS, K. ZHANG, A. VARENNE, P. GAREIL, Anal. Chim. Acta, 2006, (562)
164-170
Determination of Critical Micelle Concentrations by Capillary Electrokinetic Techniques, T. LE
SAUX, A. VARENNE, P. GAREIL, In: Electrokinetic Chromatography: Theory, Instrumentation and
Applications, U. Pyell Ed., John Wiley & Sons, New York, 2006
Characterization of Synthetic (co)Polymers by Capillary Electrophoresis, H. COTTET, P.
GAREIL, In: Capillary Electrophoresis: Methods and Protocols, Ph. Schmitt-Kopplin Ed., Humana
Press, Totowa, 2006.
In situ synthesis of monolithic stationary phases for electrochromatographic separations: study
of polymerization conditions, V. AUGUSTIN, A. JARDY, P. GAREIL, M.-C. HENNION, J.
Chromatogr. A, 2006 (1119) 80-87
Peak Shape Modeling by Haarhof - Van der Linde Function for the Determination of Correct
Migration Times: a New Insight into Affinity Capillary Electrophoresis, T. LE SAUX, A.
VARENNE, P. GAREIL, Electrophoresis, 2005 (26) 3094-3104
Evaluation of Capillary Isoelectric Focusing in Glycerol-Water Media with a View to
Hydrophobic Protein Applications, J.-M. BUSNEL, A. VARENNE, S. DESCROIX, G. PELTRE,
Y. GOHON, P. GAREIL, Electrophoresis, 2005 (26) 3369-3379
Capillary Electrophoresis Separation of Inorganic Anions in Hydro-organic Media: a Combined
Experimental and Theoretical Study of the Influence of Ion-Pairing and Solvation Phenomena,
S. DESCROIX, A. VARENNE, C. ADAMO, P. GAREIL, J. Chromatogr. A, 2004 (1032) 149-158
Frontal Analysis continuous capillary Electrophoresis Determination of Aggregation Threshold
of UV Absorbing Anionic Surfactants, T. LE SAUX, A. VARENNE, P. GAREIL, J. Chromatogr.
A, 2004 (1038) 272-282
Determination of the Aggregation Threshold of non-UV Absorbing, Neutral or Charged
Surfactants by Frontal Analysis Continuous Capillary Electrophoresis, T. LE SAUX, A.
VARENNE, P. GAREIL, J. Chromatogr. A, 2004 (1041) 219-226
Ultrashort Partial Filling Technique in Capillary Electrophoresis for Infinite Resolution of
Tramadol Enantiomers and its Metabolites with Highly Sulfated Cyclodextrins, S. RUDAZ, T.
LE SAUX, J. PRAT, P GAREIL, J.-L. VEUTHEY, Electrophoresis, 2004 (25) 2761-2771
Capillary Electrophoresis Monitoring of Halide Impurities in Ionic Liquids, D. BERTHIER, A.
VARENNE, M. DIGNE, C.-Ph. LIENEMANN, L. MAGNA, H. OLIVIER-BOURBIGOU, P.
GAREIL, The Analyst, 2004 (129) 1257-1261
Non-Aqueous Capillary Electrophoresis (NACE) of Positional Isomers of Monosulfated Fucose,
S. DESCROIX, A. VARENNE, N. GOASDOUE, J. ABIAN, M. CARRASCAL, R. DANIEL P.
GAREIL, J. Chromatogr. A, 2003 (987) 467-476
Capillary Electrophoresis Determination of the Binding Affinity of Bioactive Sulfated
Polysaccharides to Proteins. Study of the Binding Properties of Fucoidan to Antithrombin, A.
VARENNE, P. GAREIL, S. COLLIEC-JOUAULT, R. DANIEL, Anal. Biochem., 2003, (315) 152159
Influence of Electrolyte Nature on the Separation Selectivity of Amphetamines in Non-Aqueous
Capillary Electrophoresis (NACE): Protonation Degree versus Ion Pairing Effects, S.
DESCROIX, A. VARENNE, L. GEISER, S. CHERKAOUI, J.-L. VEUTHEY, P. GAREIL,
Electrophoresis, 2003 (24) 1577-1586.
Capillary Electrophoresis Profiles of Fucoidan and Heparin Fractions: Significance of Mobilitry
Dispersity for their Characterization, M.-Y. KIM, A. VARENNE, R. DANIEL, P. GAREIL, J. Sep.
Sci., 2003 (26) 1154-1162
Interaction of Fucoidan with the Proteins of the Complement Classical Pathway, B. TISSOT, B.
MONTDARGENT, L. CHEVOLOT, A. VARENNE, S. DESCROIX, P. GAREIL, R. DANIEL,
Biochim Biophys. Acta, 2003 (1651) 5-16
Determination of Lanthanum(III) and Europium(III) – Lactate Interaction Constants by
Capillary electrophoresis for Environmental Applications, S. BORTOLUS, A. VARENNE, M.
DRAYE, P. GAREIL, K. CZERWINSKI, G. COTE, Ars Separatoria Acta, 2003 (2) 29-35
Size-based Separation of Synthetic Polyelectrolytes in Entangmled Polymer Solution Capillary
Electrophoresis: the Effect of Binary Mixtures of Separating Polymers Differing in Molecular
Mass, H. COTTET, P. GAREIL, Electrophoresis, 2002 (23) 2788-2793 (special issue on fundamental
studies in separation science, invited paper)
Investigation of Mo(VI) Monomer-Dimer Equilibrium in Highly Acidic Solutions by UV
Absorbance Spectroscopy using Refined Numerical Processing, E. ESBELIN, P. GAREIL, M.
MASSON, J.-L. EMIN, Anal. Chim. Acta, 2001 (433) 299-310
On the Use of the Activation Energy Concept to Investigate Analyte and Network Deformations
in Entangled Polymer Solution Capillary Electrophoresis of Synthetic Polyelectrolytes, H.
COTTET, P. GAREIL, Electrophoresis, 2001 (22) 684-691 (special issue on CE Matrices and
Coatings, invited paper)
Determination of Mo(VI)-Zr(IV) interaction parameters in highly acidic solutions by UV
absorbance spectrophotometry, E. ESBELIN, P. GAREIL, M. MASSON, J.-L. EMIN, Anal. Chim.
Acta, 2001 (442) 171-182
Regioselective desulfation of sulfated L-fucopyranoside by a new sulfoesterase from the marine
molusk Pecten maximus. Application to the structural study of algal fucoidan, R DANIEL, O.
BERTEAU, L. CHEVOLOT, A. VARENNE, P. GAREIL, N. GOASDOUE, Eur. J. Biochem., 2001
(268) 5617-5626
Capillary Electrophoresis of Associative Diblock Copolymers, H COTTET, P. GAREIL, P.
GUENOUN, F. MULLER, M. DELSANTI, P. LIXON, J. W. MAYS, J YANG, J. Chromatogr. A,
2001 (939) 109-121
From Small Charged Molecules to Oligomers: a Semi Empirical Approach to the Modeling of
Actual Mobility in Free Solution, H. COTTET, P. GAREIL, Electrophoresis, 2000 (21) 1493-1504
A Semi Empirical Approach to the Modeling of the Electrophoretic Mobility in Free Solution:
Application to polystyrenesulfonates of various sulfonation rates, H. COTTET, P. GAREIL, O.
THEODOLY, C. E. WILLIAMS, Electrophoresis, 2000 (21) 3529-3540
Association between Protein Particles and Long Amphiphillic Polymers: Effect of the Polymer
Hydrophobicity on Binding Isotherms, I. PORCAR, H. COTTET, P. GAREIL, C. TRIBET,
Macromolecules, 1999 (32) 3922-3929
Identification
of
Heparin
Oligosaccharides
by
Direct
Coupling
of
Capillary
Electrophoresis/Ionspray-Mass Spectrometry, S. DUTEIL, P. GAREIL, S. GIRAULT, A.
MALLET, C. FEVE, L. SIRET, Rapid Commun. Mass Spectrom., 1999 (13) 1889-1898
Development of a Capillary Electrophoresis Assay Based on Free Sulfate Determination for the
Direct Monitoring of Sulfoesterase Activity, S. SAILLARD, P. GAREIL, J. JOZEFONVICZ, R.
DANIEL, Anal. Biochem., 1999 (275) 11-21
The Effect of Blob Size and Network Dynamics on the Size-Based Separation of
Polystyrenesulfonates by Capillary Electrophoresis in the Presence of Entangled Polymer
Solutions, H. COTTET, P. GAREIL, J-L. VIOVY, Electrophoresis, 1998 (19) 2151-2162
Le point sur les méthodes de séparation électrocinétiques capillaires, P. GAREIL, A. VARENNE,
H. COTTET, La Lettre de la Société Française d'Electrophorèse, septembre 1998, pp 2-3
Formation of Complexes between Protein Particles and Long Amphiphilic Polymers: Binding
Isotherms versus Size and Surface of the Particles, I. PORCAR, P. GAREIL, C. TRIBET, J. Phys.
Chem. B, 1998 (102) 7906-7909
Selectivity in Capillary Electrophoresis: Application to Chiral separations with Cyclodextrins, F.
LELIEVRE, P. GAREIL, A. JARDY, Anal. Chem., 1997 (69) 385-392
Intrinsic Selectivity in Capillary Electrophoresis for Chiral Separations with Dual Cyclodextrin
Systems, F. LELIEVRE, P. GAREIL, Y. BAHADDI, H. GALONS, Anal. Chem., 1997 (69) 393-401
Electrophoretic Behaviour of Fully Sulfonated Polystyrenes in Capillaries Filled with Entangled
Polymer Solutions, H. COTTET, P. GAREIL, J. Chromatogr. A, 1997 (772) 369-384
Use of Zwiterionic Cyclodextrins as a Chiral Agent for the Separation of Enantiomers by
Capillary Electrophoresis, F. LELIEVRE, C. GUEIT, P. GAREIL, Y. BAHADDI, H. GALONS,
Electrophoresis, 1997 (18) 891-896, (Special issue on Capillary Electrophoresis of Chiral Compounds,
invited paper)
Le point sur l'instrumentation en électrophorèse capillaire : une technologie au service d'un
large éventail de méthodes, P. GAREIL, Spectra Analyse, 1997 (198) 32-37
Preparation and Complexation Ability of Zwitterionic Derivatives of Cyclodextrins, Y.
BAHADDI, F. LELIEVRE, P. GAREIL, J. MAIGNAN, H. GALONS, Carbohydr. Res., 1997 (303)
229-32
Micellar Electrokinetic Chromatography of Long Chain Saturated and Unsaturated Free Fatty
Acids with Neutral Micelles. Considerations regarding Selectivity and Resolution Optimization,
J. COLLET, P. GAREIL, J. Chromatogr. A, 1997 (792) 165-177, (Special issue on Selectivity in
Capillary Electrophoresis , invited paper)
Capillary Electrochromatography: Operating characteristics and Enantiomeric Separations, F.
LELIEVRE, C. YAN, R. N. ZARE, P. GAREIL, J. Chromatogr. A, 1996 (723) 145-156
Quantification of PCR Amplified DNA Fragments by Capillary Electrophoresis using Laser
Induced Fluorescence Detection, U. VINCENT, G. PATRA, J. THERASSE, P. GAREIL,
Electrophoresis, 1996 (17) 512-517
Direct Determination of Iron(II), Iron(III) and Total Iron as UV-Absorbing Complexes by
Capillary Electrophoresis, S. SCHÄFFER, P. GAREIL, C. DEZAEL, D. RICHARD, J. Chromatogr.
A, 1996 (740) 151-157
Chiral Separations of Underivatized Arylpropionic Acids by Capillary Electrophoresis with
Various Cyclodextrins; Acidity
and Inclusion Constant Determinations, F. LELIEVRE, P.
GAREIL, J. Chromatogr. A, 1996 (735) 311-320 (special issue on Capillary Electrophoresis of Drugs,
invited paper)
Use of Neutral Surfactants for the Capillary Electrophoresis Separation of Hydrophobically
Modified Poly(acrylic acids), J. COLLET, C. TRIBET, P. GAREIL, Electrophoresis, 1996 (17)
1202-1209
Capillary Zone Electrophoresis Separation of C14 - C18 Linear Saturated and Unsaturated Free
Fatty Acids with Indirect UV Detection, J. COLLET, P. GAREIL, J. Cap. Elec., 1996 (3) 77-82
Capillary Zone Electrophoresis Determination of C2-C18 Linear Saturated Free Fatty Acids
with Indirect Absorbance detection, R. ROLDAN-ASSAD, P. GAREIL, J. Chromatogr. A, 1995
(708) 339-350
Capillary Electrophoresis Determination of Degradation Products of Nitrilotriacetic Acid used
as Complexing Agent in a Desulphuration Process, S. SCHÄFFER, P. GAREIL, L. CARPOT, C.
DEZAEL, J. Chromatogr. A, 1995 (717) 351-362
Capillary Zone Electrophoresis with Indirect UV Detection Applying a UV-absorbing Counterion, J.COLLET, P. GAREIL, J. Chromatogr. A, 1995 (716) 115-122
Determination of the Formation Constant for the Inclusion Complex Between Rutin and Methyl-cyclodextrin, S. LETELLIER, B. MAUPAS, J. P. GRAMOND, F. GUYON, P. GAREIL, Anal.
Chim. Acta, 1995 (315) 357-363
Electrophorèse, P. GAREIL, G. PELTRE, Techniques de l'Ingénieur, Traité Analyse et
Caractérisation, Séparations électrochimiques, P1815, Paris, 1995
Séparations d'énantiomères par les techniques électrocinétiques capillaires, F. LELIEVRE, P.
GAREIL, M. CAUDE, Analusis, 1994 (22) 413-429
5.6 Distinctions obtained by members of the team
- Anne Varenne: Award 2004, Division Chimie Analytique de la Société Française de
Chimie
6. Equipment
-
4 capillary electrophoresis systems
-
1 mass spectrometer hyphenated to capillary electrophoresis
7. Former Ph.D. students
Ramiz ROLDAN-ASSAD, juin 1994
Behaviour of ionic surfactants in capillary electrophoresis: use as surface agents and for
analytical separations
present position: pharmaceutical company in Mexico
Frédéric LELIEVRE, February, 1996
Enantioselectivity of cyclodextrins in capillary electrophoresis and in electrochromatography
present position: Sanofi-Aventis, Aramon, France
Ursula VINCENT, July, 1996
Separation of polynucleotids and double-strand DNA fragments in capillary electrophoresis:
Application to biological samples
present position: Researcher at the Joint Research Center of the European Commission,
Institute for Reference Materials and Measurements, Geel, Belgium
Jérôme COLLET, December, 1996
Application of capillary electrophoresis to the analysis and characterization of surfactants
and amphiphilic polymers
Hervé COTTET, July, 1999
Physicochemical characterization of polyelectrolytes by capillary electrophoresis
present position: Assistant Professor, Laboratoire Organisation Moléculaire : Evolution et
Matériaux Fluorés, CNRS-University of Montpellier II, France
Eric ESBELIN, September, 1999
Study of the complexation and precipitation of molybden(VI) by zirconium(IV) in highly
acidic media: application to the dissolution of irradiated nuclear fuel
present position: CEA, Marcoule, France
Séverine DUTEIL, March, 2000
Separation and characterization of heparin fragments by capillary electrophoresis coupled to
mass spectrometry
present position: Sanofi-Aventis, Vitry sur Seine, France
Stéphanie DESCROIX, November, 2002
Evaluation of organic and hydro-organic media for capillary electrophoresis
present position: Research Scientist CNRS, Laboratoire Environnement et Chimie
Analytique, ESPCI, Paris, France
Sophie BORTOLUS, September, 2004
Study of the behaviour of humic acids in solution and of their interaction with copper(II) and
lanthane(III) ions
Thomas LE SAUX, November, 2004
Determination of non-covalent interaction parameters in solution by capillary electrophoretic
methods. Application fields and performances. Rationalization of protocols.
present position: Assistant Professor, Département de Chimie, Processus d'Activation Sélectif
par Transfert d'Energie Uni électronique ou Radiative (PASTEUR), Paris, France
Yannis FRANÇOIS, November, 2006
Ionic solvents for miniaturized electrokinetic separations.
present position: Contractual assistant professor, Laboratoire de Chimie et Biochimie
Pharmacologiques et Toxicologiques, University of Paris V, France
8. Last post-doctoral fellows
Iolanda PORCAR, 1998-2000
Quantitative study of the interactions between proteins and amphiphilic polyelectrolytes by
capillary electrophoresis
present position: Professor, University of Valencia, Spain
Kai ZHANG (University of Beijin, China), 2002-2003
From molecular solvents to ionic solvents: new media for electrokinetic analytical
separations
present position: Post-doctoral fellowship, Washington State University, Pullman, WA, USA
Marek URBANEK (University of Brno, Czech Republic), 2005
Development of new methods for trace analysis of metal cations in aqueous, industrial and
environmental matrices
present position: Spectronex, Prague, Czech Republic