ERGECD 2014

ERGECD 2014
June 12–13
Paris, France
25th meeting of the European Research Group on
Experimental Contact Dermatitis
www.ergecd2014.u-psud.fr
Conference venue
Université de Paris Descartes
Faculté de Pharmacie, Amphithéâtre Guignard
4 avenue de l’Observatoire - Paris
Program th
Wednesday 11 June 2014 19h00-­‐22h00 Get together and welcome to Paris (‘La terrasse des Lauriers’) Thursday, 12th June 2014 9h30-­‐9h45 Welcome to Paris and introduction to sessions (S. Kerdine-­‐Römer & M. Pallardy) Session 1: Skin penetration, chemical reactivity and metabolism Chairs: Jean-­‐Pierre Lepoittevin and Aurelia Del Bufalo 9h45-­‐10h00 Andreas Natsch, Givaudan, CH: Linalool and limonene in fragranced products: Stability and quantification of potentially sensitizing hydroperoxides 10h00-­‐10h15 Pete Boyd, Erika Parkinson, Chris Pickard, Richard Cubberley, Peter Friedmann, Maja Aleksic, Neil Smyth, Eugene Healy, Paul Skipp, Molsheim, DE: Hapten transfer by thiolysis: a novel mechanism in hapten-­‐mediated allergies? 10h15-­‐10h30 Camille Debeuckeleare, Elena Giménez-­‐Arnau, Jean-­‐Pierre Lepoittevin, Strasbourg -­‐ FR: Methylisothiazolinone: in situ reactivity in reconstructed human epidermis 10h30-­‐10h45 Eric Moss, Valérie Berl, F.-­‐M. Moussallieh, K. Elbayed, I.-­‐J. Namer and Jean-­‐Pierre Lepoittevin, Laboratoire de Dermatochimie, Strasbourg, FR: Investigation of prohapten activations in Reconstructed Human Epidermis: the case of 2-­‐methoxy-­‐4-­‐methylphenol 10h45-­‐11h15 Coffee Break 1 11h15-­‐11h30 David W. Roberts, Manchester -­‐ UK: DNCB and related chemicals – what more can we learn from them? 11h30-­‐11h45 Sandrine Jacquoilleot, Maja Aleksic, Dean Naisbitt , Safety & Environmental Assurance Centre (SEAC), Colworth Science Park, Sharnbrook, Bedford, UK: Understanding the role of skin metabolism in Allergic Contact Dermatitis: Glutathione as a clearance system 11h45-­‐12h00 Andreas Schepky, Carine Jacques-­‐Jamin, Richard Cubberley, Hélène Duplan, Joan Eilstein, Daniela Gerstel, Sébastien Grégoire, Nicky Hewitt, Martina Klaric, Helga Rothe, Cosmetics Europe Member Task Force Skin Bioavailability, Hamburg, DE: Comparison of protocols measuring stratum corneum diffusion/partition coefficients and skin penetration with focus on sensitizers 12h00-­‐13h15 Lunch Session 2: Innate Immune Response Chairs: Fanny Boislève & Emanuela Corsini 13h15-­‐13h30 Dessy Rachmawati, Hetty J. Bontkes, B.M.E. von Blomberg, Rik J. Scheper, Ingrid M.W. van Hoogstraten, Amsterdam, The Netherlands: Innate stimulatory capacity of high molecular weight transition metals: Au (gold) and Hg (mercury) 13h30-­‐13h45 Emanuela Corsini1, Aikaterini Berdiaki2, Valentina Galbiati1, Angela Papale1, Rafaela-­‐
Maria Kavasi2, Aristidis Tsatsakis3, George N Tzanakakis2, Dragana Nikitovic2, Milano, IT1 & Heraklion, GR2&3: Hyaluronan regulates chemical allergen-­‐induced IL-­‐18 response in human keratinocytes 13h45-­‐14h00 Brunhilde Blömeke, Department of Environmental Toxicology, Trier, DE: Characterization of our HaCaT/THP-­‐1 coculture model 14h00-­‐14h15 Jenny Hennen, Department of Environmental Toxicology, Trier, DE: Application of the HaCaT/THP-­‐1 coculture model to assess the impact of keratinocytes on chemical-­‐
induced activation of dendritic cells 14h15-­‐14h30 Ilona Kosten, Sander Spiekstra, Tanja de Gruijl, Sue Gibbs, Amsterdam, NL: Comparison of early innate response in skin and gingiva equivalents: a different role for CXCL12 14h30-­‐14h45 Ian Kimber, Laura H Eaton, Aleksandra Metryka, Ruth A Roberts and Rebecca J Dearman, Manchester, UK: Cytokine requirements for Langerhans’ cell migration: not all allergens are equal 14h45-­‐15h00 Roger Emter, Givaudan, CH: Dual regulation of sensitizer-­‐induced HMOX1 expression by BACH1 and Nrf2: Comparison to regulation of the AKR1C2-­‐ARE element in KeratinoSensTM 15h00-­‐15h15 Zeina El Ali, Claudine Deloménie, Marcel Bonay, Marc Pallardy and Saadia Kerdine-­‐Römer, Paris, FR: Nrf2 protects dendritic cells against death induced by chemicals sensitizers 15h15-­‐15h30 Zeina El Ali, Patrice Hemon, Stefan F. Martin, Marc Pallardy and Saadia Kerdine-­‐
Römer, Paris, FR: How Nrf2 controls allergic contact dermatitis in response to contact sensitizers? 15h30-­‐15h45 Marie de Bourayne, Zeina El Ali, O. Filhol, C. Cochet C., Marc Pallardy and Saadia Kerdine-­‐Römer, Paris, FR: CK2 : a novel kinase involved in dendritic cells activation in response to contact sensitizers 15h45-­‐16h15 Coffee Break 2 Session 3: Adaptative Immune Response Chairs: Jean-­‐François Nicolas & Stefan Martin 16h15-­‐16h30 Rebecca J Dearman, Mark D Hayes and Ian Kimber, Manchester, UK: Chloromethylisothiazolinone/methylisothiazolinone: contact or respiratory allergen? 16h30-­‐16h45 Marie Eliane Azoury1, Noémie Scornet2, Stéphanie Delluc3, Sandrine Delarue-­‐Cochin2, Cathy Nhim1, Bernard Maillère4, Richard Weaver5, Nancy Claude5, Delphine Joseph2, Marc Pallardy1. 1,2Châtenay-­‐Malabry, FR; 3Lyon, FR; 4Saclay, FR; 5Suresnes, FR: Identification and Frequency of Naïve T Lymphocytes Specific for Penicillin: Implication in drug-­‐allergy 16h45-­‐17h00 Aurelie Guironnet-­‐Paquet, Marc Vocanson, Jean-­‐François Nicolas, Olivier Hecquet, Lyon, FR: CD8+ T suppressor cells are major players of skin tolerance to haptens 17h00-­‐17h15 Ida B Niklasson, Niamh O´Boyle, Ali Tehrani-­‐Bagha, Tamara Delaine, Krister Holmberg, Kristina Luthman, and Ann-­‐Therese Karlberg, Göteborg, SE: Epoxy Resins Monomers with Reduced Skin Sensitizing Potency 17h15-­‐17h30 Amy Popple, Gavin Maxwell, Nichola Gellatly, Jason Williams, Rebecca J Dearman and Ian Kimber, Manchester -­‐ UK: Characterisation of lymphocyte proliferative responses in patients sensitised to paraphenylenediamine or methlylisothiazolinone 17h30-17h45 Jean-­‐François Nicolas, Aurore Rozières, Bernard Bensaid, Frédéric Berard, Marc Vocanson, Lyon, FR: Allergic or irritant contact dermatitis. How to make the difference?
17h45-­‐18h00 Angèle Cortial, Marc Vocanson, Aurore Rozières, Catherine Goujon, Marie Baeck, Stéphanie Briançon, Jean-­‐François Nicolas, Lyon, FR: New vectors for the immunobiological diagnosis of allergic contact dermatitis 19h30 Dinner Friday, 13th June 2014 Session 4: Models and case studies Chairs: Silvia Martinozzi-­‐Teissier & David Basketter 9h30-­‐9h45 David Ponting, Tamara Delaine, Ida Niklasson, Roger Emter, Lina Hagvall, Per-­‐Ola Norrby, Kristina Luthman, Andreas Natsch and Ann-­‐Therese Karlberg. University of Gothenburg, SE: Completing the picture -­‐ using in silico, chemico, vivo and vitro data together to investigate sensitizing potency -­‐ a case study on epoxy-­‐alcohols and their metabolites 9h45-­‐10h00 Carsten Goebel, Pieter-­‐Jan Coenraads, John Troutman, Jennifer Hennen, Laura Pot, Helga Rothe, Harald Schlatter, Frank G. Gerberick, Brunhilde Bloemeke, Darmstad, DE: The impact of PPD side-­‐chain modification on skin sensitization induction and cross-­‐
elicitation: example 2-­‐methoxymethyl-­‐PPD 10h00-­‐10h15 H. Ivanova1, Ann Detroyer2, Cécile Piroird2, Charles Gomes2, Joan Eilstein2, I. Popova1, C. Kuseva1, Y. Karakolev1, S. Dimitrov1, O. Mekenyan1, 1Burgas, Bulgaria; 2Aulnay-­‐sous-­‐
Bois, FR: Variability in data from skin sensitization (LLNA) and reactivity (DPRA) testings 10h15-­‐10h30 David Basketter, John Mc Fadden, Ian White & Ian Kimber, UK: Thought on positive controls substance /the issue of HCA 10h30-­‐10h45 Marc A.T. Teunis, Sander W. Spiekstra, Mieke Smits, Els Adriaens, Tobias Eltze, Valentina Galbiati, Cyrille Krul, Robert Landsiedel, Raymond Pieters, Judith Reinders, Erwin Roggen, Emanuela Corsini, Susan Gibbs. Utrecht -­‐ NL: International ring trial of the epidermal equivalent sensitizer potency assay: reproducibility and predictive-­‐
capacity 10h45-­‐11h15 Coffee Break 3 11h15-­‐11h30 Ilona Kosten1, Sander W. Spiekstra1, Emanuela Corsini3, Valentina Galbiati3, Erwin Roggen4, Susan Gibbs1,2, 1,2Amsterdam, The Netherlands; 3Milano, Italy; 43Rs Management and Consultancy, Denmark: An Epidermal Equivalent Assay For Identification And Ranking Potency Of Contact Sensitizers: assessment of metal sensitizers 11h30-­‐11h45 Françoise Cottrez1, E. Boitel1, Jean-­‐Claude Ourlin2, Jean-­‐Luc Peiffer2, Isabelle Fabre2, Imène-­‐Sarah Henaoui3, Bernard Mari3, Pascal Barbry3, and Hervé Groux1, 1Grasse, FR 2
Vendargues, FR, 3Sophia Antipolis, FR: Assessment of SENS-­‐IS®, a 3D reconstituted epidermis based model for quantifying chemical sensitization potency : results of an inter-­‐laboratories study 11h45-­‐12h00 Aurélia Del Bufalo, Fleur Tourneix, Silvia Martinozzi-­‐Teissier, Aulnay-­‐sous-­‐Bois, FR: Evaluation of SENS-­‐IS®, an Episkin® based model, for the prediction of skin sensitization on a set of cosmetic ingredients 12h00-­‐12h15 Virginie Mutez1, Marc Vocanson 1, Philipp Esser2, Martina Klaric3, Stefan Martin2, Jean-­‐
François Nicolas1, 1Lyon, FR; 2Freiburg, DE; 3Bruxelles, B: Optimization of the human T Cell Priming Assay for the identification of chemical allergens 12h15-­‐12h30 David W. Roberts, Manchester, UK: Can we get EC3 values from the rLLNA? 12h30-­‐14h00 Lunch Session 5: Integrated approaches for skin sensitization assessment Chairs: Pierre Jacques Ferret & Saadia Kerdine-­‐Römer 14h00-­‐14h15 Gavin Maxwell., R Cubberley , S Dhadra , N Gellatly , S Jacquoilleot , C Moore , R Pendlington , J Pickles , O Saib, D Sheffield , R Stark , V Summerfield. and C MacKay, Colworth Science Park, Sharnbrook, Bedford, UK -­‐ From Pathways to People: Applying the Skin Sensitisation AOP to Risk Assessment 14h15-­‐14h30 Annette Mehling, Dusseldorf, DE, Skin sensitization hazard identification: The simplicity of twos of threes 14h30-­‐14h45 Silvia Martinozzi-­‐Teissier, Thierry Pauloin, Aurelia Del Bufalo, C Piroird, C Gomes, A Detroyer, J Eilstein, Françoise Rousset, Nathalie Alépée, Jacques Clouzeau, Aulnay sous Bois, FR: Combination of in silico and in vitro tools for the evaluation of Skin Sensitization: application to cosmetic industrial needs 14h45-­‐15h00 N Gellatly , R Cubberley , S Dhadra , S Jacquoilleot , G Maxwell , C Moore , R Pendlington , J Pickles , O Saib , D Sheffield , R Stark , V Summerfield and C MacKay, Colworth Science Park, Sharnbrook, Bedford, UK: Modelling the Skin Sensitisation Adverse Outcome Pathway (AOP) for Risk Assessment 15h00-­‐15h15 David Basketter & Ian Kimber, Manchester, UK: Validation of in vitro alternatives for sensitization – what have we learned ? 15h15-­‐16h00 Round Table What are the scientific questions still needed to be solved? How can we address the question of risk-­‐assessment using in vitro test batteries? (All, chaired by M. Pallardy, Châtenay-­‐Malabry, FR) 4