R.Pini, G.Agati, P.Matteini, F.Ratto, F.Rossi, D.Fedele

Transcription

Biophotonics and Nanomedicine Lab – IFAC CNR, Sesto Fiorentino
(R.Pini, G.Agati, P.Matteini, F.Ratto, F.Rossi, D.Fedele)
CNR - Commessa del Dip. Materiali & Dispositivi
INT.P07.001 – Biofotonica e Optoelettronica per l’Innovazione Industriale
Innovation transfer of biophotonic technologies to
industries
Development of new laser devices and delivery
systems for surgery and therapy
Preclinical and clinical studies on optoelectronic
tools in minimally invasive surgery and therapy
(ophthalmology, neuro and microvascular surgery,
dermatology)
Development of gold nanoparticles as laser-activated
nano chromophores for medical diagnosis and
therapy
Multispectral microfluorimetry, in situ spectrofluorimetry, SHG e AF microscopies of biomolecules
Theoretical and experimental studies on laserbiotissue interaction
Preclinical and clinical studies on laser surgery
MINI-mally invasive surgery
(In cooperation with the Ophthalmic Dept. of Azienda USL 4, Prato)
MICRO-surgery
Diode Laser Welding of the Cornea (as a substitute of conventional
stitching):
NANO-surgery
LASER day 30
Preclinical Studies in animal
models with histological exams
Clinical Experimentation of
Corneal laser welding in the
transplant of the cornea (>70
patients treated)
Advantages: shorter surgery
times, less inflammation, faster
healing = minimally invasiveness
immunohist
Preclinical and clinical studies on laser surgery
MICRO-surgery
Femtosecond Laser sculpturing of the cornea in the transplant
(In cooperation with the Ophthalmic Dept. of Azienda USL 4, Prato and Bascom Palmey Eye institute, Miami)
8.5 mm
300
µm
Suturing by laser welding
Diode laser-assisted microvascular anastomosis in neurosurgery
(In cooperation with the Neurosurgery Inst. of the Catholic Univ.,
Univ., Rome)
Laser suturing of a 0.5mm artery
Laser suturing in a by-pass
Histology after laser, day 90
Wiegert-elastica-Van Gieson,
Preclinical studies on laser surgery
NANO-surgery
(In cooperation with the Fisiopathologic Clinics Dept. of the University of Florence)
Synthesis of Gold Nano Rods absorbing light in the Near Infrared
Functionalisation and laser-activation of the Nano Rods
Biocompatible coating with
PEG and Silica
Laser Welding of ocular tissues
using Nano Rods as photo
enhancing chromophores
Preclinical studies on laser surgery
NANO-surgery for tumor diagnostics and therapy
Optoacoustic diagnosis of cancer cells
Photothermal + photoacoustic therapies
Upon systemic delivery, the functionalised gold
nanordods will target the cancer cells
selectively. Then they will be irradiated by a nearinfrared laser, to generate diagnosis, imaging and
therapy of cancer:
1) At low laser power, the photoacoustic effects will
result into the generation of ultrasound, which will
be used for the molecular diagnosis and
imaging of the cancer cells, even in small
metastases;
2) At higher laser power, the photothermal and
photoacoustic response will lead to the formation
of cavitation micro-bubbles, which will damage
the membranes and induce the apoptosis of the
cancer cells with high efficiency, selectivity and
non-invasiveness.
Modeling and microscopy of laser-tissue interaction
Studies on laser-induced transitions in collagen
Models of photothermal
dynamics by FEM analysis
Microfluorescence imaging
analysis, polarization
microscopy, SEM and TEM
Multiphoton microscopy of corneal tissue under laser therapies
(In cooperation with the Biophotonicss Lab at LENS, Sesto Fiorentino)
Fiorentino)
Fibrous corneal collagen exhibits a
large Second Harmonic (SHG) signal
SHG and Two Photon Fluorescence
are powerful tools to characterise
corneal modifications and diseases
60 X 60 µm2
Biomolecules multispectral microfluorimetry
Localization of polyphenols in plant tissues
Confocal laser scanning image and
spectrum, excitation at 488 nm
Mesophyll cells of a Ligustrum vulgare leaf
at a depth of 20 µm from the adaxial
surface:
red = clorophyll
green = flavonoids
Epifluorescence microscope,
excitation at 488 nm
Flavonoids in a Cistus salvifolius
trichome
50 µm
Biomolecules in situ spectrofluorimetry
Chlorophyll fluorescence (ChlF) as index of fruit ripening
White light
photos
Fluorescence images
green excitation
red excitation
Log
ChlF exc 650
ChlF exc 550
Image
elaboration
Maturation index
Innovation transfer of Biophotonic Technologies
Organisation of a Biophotonic Cluster in
Tuscany involving enterprises, research
centers and public hospitals (coord. by IFAC)
Industrial research contracts on surgical
diode lasers and LEDs, surgical hand pieces,
ophthalmic diagnostic instrumentation
11 CNR Patents (in 2005-2008):
4 Italian Patents
5 PCT (EU) Patents
2 USA Patents
Common Research Lab with the main Italian
company of biomedical lasers (EL.EN. SpA)
[email protected]
RECENT
RECENT PUBLICATIONS
PUBLICATIONS
1.
1. P.
P. MATTEINI,
MATTEINI, F.
F. RATTO,
RATTO, F.
F. ROSSI,
ROSSI, R.
R. CICCHI,
CICCHI, C.
C. STRINGARI,
STRINGARI, D.
D. KAPSOKALYVAS,
KAPSOKALYVAS, F.S.
F.S. PAVONE,
PAVONE, R.
R. PINI
PINI (2009).
(2009). PhotothermallyPhotothermallyinduced
induced disordered
disordered patterns
patterns of
of corneal
corneal collagen
collagen revealed
revealed by
by SHG
SHG imaging,
imaging, OPTICS
OPTICS EXPRESS
EXPRESS Vol.
Vol. 17,
17, pp.4868-4878.
pp.4868-4878.
2.
2. F.
F. RATTO,
RATTO, P.
P. MATTEINI,
MATTEINI, F.
F. ROSSI,
ROSSI, L.
L. MENABUONI,
MENABUONI, N.
N. TIWARI,
TIWARI, S.
S. K.
K. KULKARNI,
KULKARNI, R.
R. PINI
PINI (2009).
(2009). Photothermal
Photothermal effects
effects in
in
connective
tissues
mediated
by
laser-activated
gold
nanorods.
NANOMEDICINE:
NANOTECHNOLOGY,
BIOLOGY
AND
MEDICINE,
connective tissues mediated by laser-activated gold nanorods. NANOMEDICINE: NANOTECHNOLOGY, BIOLOGY AND MEDICINE, DOI:
DOI:
10.1016/j.nano.2008.10.002.
10.1016/j.nano.2008.10.002.
3.
3. G.
G. AGATI,
AGATI, M.
M. L.
L. TRAVERSI,
TRAVERSI, Z.
Z. G.
G. CEROVIC
CEROVIC (2008).
(2008). Chlorophyll
Chlorophyll fluorescence
fluorescence imaging
imaging for
for the
the non-invasive
non-invasive assessment
assessment of
of
anthocyanins
in
whole
grape
(Vitis
vinifera
L.)
bunches.
PHOTOCHEMISTRY
AND
PHOTOBIOLOGY
84:
1431–1434.
anthocyanins in whole grape (Vitis vinifera L.) bunches. PHOTOCHEMISTRY AND PHOTOBIOLOGY 84: 1431–1434.
4.
4. G.
G. AGATI,
AGATI, Z.
Z. G.
G. CEROVIC,
CEROVIC, A.
A. DALLA
DALLA MARTA,
MARTA, V.
V. DI
DI STEFANO,
STEFANO, P.
P. PINELLI,
PINELLI, M.
M. L.
L. TRAVERSI
TRAVERSI AND
AND S.
S. ORLANDINI
ORLANDINI (2008).
(2008). OpticallyOpticallyassessed
assessed preformed
preformed flavonoids
flavonoids and
and susceptibility
susceptibility of
of grapevine
grapevine to
to Plasmopara
Plasmopara viticola
viticola under
under different
different light
light regimes.
regimes. FUNCTIONAL
FUNCTIONAL
PLANT
PLANT BIOLOGY
BIOLOGY 35:
35: 77-84.
77-84.
5.
F.
ROSSI,
P.
MATTEINI,
5. F. ROSSI, P. MATTEINI, F.
F. RATTO,
RATTO, L.
L. MENABUONI,
MENABUONI, I.I. LENZETTI,
LENZETTI, R.
R. PINI
PINI (2008).
(2008). Laser
Laser tissue
tissue welding
welding in
in ophthalmic
ophthalmic surgery.
surgery.
JOURNAL
JOURNAL OF
OF BIOPHOTONICS,
BIOPHOTONICS, vol.1,
vol.1, p.
p. 331-342.
331-342.
6.
6. P.
P. MATTEINI,
MATTEINI, F.SBRANA,
F.SBRANA, B.
B. TIRIBILLI,
TIRIBILLI, R.
R. PINI.
PINI. (2008).
(2008). Atomic
Atomic force
force microscopy
microscopy and
and transmission
transmission electron
electron microscopy
microscopy analyses
analyses
of
low-temperature
laser
welding
of
the
cornea.
LASERS
IN
MEDICAL
SCIENCE,
doi:
10.1007/s10103-008-0617-4.
of low-temperature laser welding of the cornea. LASERS IN MEDICAL SCIENCE, doi: 10.1007/s10103-008-0617-4.
7.
7. F.
F. ROSSI,
ROSSI, P.
P. MATTEINI,
MATTEINI, I.I. BRUNO,
BRUNO, P.
P. NESI,
NESI, R.
R. PINI
PINI (2007).
(2007). Monitoring
Monitoring thermally-induced
thermally-induced phase
phase transitions
transitions in
in porcine
porcine cornea
cornea with
with
the
the use
use of
of fluorescence
fluorescence micro-imaging
micro-imaging analysis.
analysis. OPTICS
OPTICS EXPRESS,
EXPRESS, Vol.
Vol. 15,
15, Issue
Issue 18,
18, pp.
pp. 11178-11184.
11178-11184.
8.
8. F.ROSSI,
F.ROSSI, R.PINI,
R.PINI, L.MENABUONI
L.MENABUONI (2007).
(2007). Experimental
Experimental and
and model
model analysis
analysis on
on the
the temperature
temperature dynamics
dynamics during
during diode
diode laser
laser
welding
of
the
cornea.
JOURNAL
OF
BIOMEDICAL
OPTICS
12,
01403-1/-7.
welding of the cornea. JOURNAL OF BIOMEDICAL OPTICS 12, 01403-1/-7.
9.
9. P.MATTEINI,
P.MATTEINI, F.ROSSI,
F.ROSSI, L.MENABUONI,
L.MENABUONI, AND
AND R.PINI
R.PINI (2007).
(2007). Microscopic
Microscopic Characterization
Characterization of
of Collagen
Collagen Modifications
Modifications Induced
Induced by
by
Low-Temperature
Diode-Laser
Welding
of
Corneal
Tissue.
LASERS
IN
SURGERY
AND
MEDICINE
39:597–604).
Low-Temperature Diode-Laser Welding of Corneal Tissue. LASERS IN SURGERY AND MEDICINE 39:597–604).
10.
10. R.MENCUCCI,
R.MENCUCCI, C.MAZZOTTA,
C.MAZZOTTA, F.ROSSI,
F.ROSSI, C.PONCHIETTI,
C.PONCHIETTI, R.PINI,
R.PINI, S.BAIOCCHI,
S.BAIOCCHI, A.CAPOROSSI,
A.CAPOROSSI, U.MENCHINI
U.MENCHINI (2007).
(2007). Riboflavin
Riboflavin and
and
ultraviolet
ultraviolet A
A collagen
collagen crosslinking:
crosslinking: In
In vivo
vivo thermographic
thermographic analysis
analysis of
of the
the corneal
corneal surface.
surface. JJ CATARACT
CATARACT REFRACT
REFRACT SURG,
SURG, 33:1005–
33:1005–
1008.
1008.
11.
11. G.
G. AGATI,
AGATI, S.
S. MEYER,
MEYER, P.
P. MATTEINI,
MATTEINI, Z.
Z. G.
G. CEROVIC
CEROVIC (2007).
(2007). Assessment
Assessment of
of anthocyanins
anthocyanins in
in grape
grape (Vitis
(Vitis vinifera
vinifera L.)
L.) berries
berries using
using aa
non-invasive
non-invasive chlorophyll
chlorophyll fluorescence
fluorescence method.
method. J.
J. AGRIC.
AGRIC. FOOD
FOOD CHEM.
CHEM. 55:
55: 1053-1061.
1053-1061.
12.
12. G.
G. AGATI,
AGATI, P.
P. MATTEINI,
MATTEINI, A.
A. GOTI
GOTI &
& M.TATTINI
M.TATTINI (2007).
(2007). Chloroplast-located
Chloroplast-located flavonoids
flavonoids can
can scavenge
scavenge singlet
singlet oxygen.
oxygen. NEW
NEW
PHYTOLOGIST
174:
77–89
PHYTOLOGIST 174: 77–89
13.
13. A.PUCA,
A.PUCA, A.ALBANESE,
A.ALBANESE, G.ESPOSITO,
G.ESPOSITO, G.MAIRA,
G.MAIRA, MD,
MD, B.TIRPAKOVA,
B.TIRPAKOVA, G.ROSSI,
G.ROSSI, A.MANNOCCI,
A.MANNOCCI, R.PINI
R.PINI (2006).
(2006). Diode
Diode laser-assisted
laser-assisted
carotid
carotid bypass
bypass surgery:
surgery: an
an experimental
experimental study
study with
with morphological
morphological and
and immunohistochemical
immunohistochemical evaluations.
evaluations. NEUROSURGERY
NEUROSURGERY Vol.
Vol. 59
59
N.6,
pp.
1286-1294.
N.6, pp. 1286-1294.
14.
14. M.TATTINI,
M.TATTINI, D.
D. REMORINI,
REMORINI, P.PINELLI,
P.PINELLI, G.
G. AGATI,
AGATI, E.
E. SARACINI,
SARACINI, M.
M. L.
L. TRAVERSI,
TRAVERSI, R.
R. MASSAI
MASSAI (2006).
(2006). Morpho-anatomical,
Morpho-anatomical,
physiological
physiological and
and biochemical
biochemical adjustments
adjustments in
in response
response to
to root
root zone
zone salinity
salinity stress
stress and
and high
high solar
solar radiation
radiation in
in two
two Mediterranean
Mediterranean
evergreen
shrubs,
Myrtus
communis
L.
and
Pistacia
lentiscus
L.
NEW
PHYTOLOGIST,
170,
779-794.
evergreen shrubs, Myrtus communis L. and Pistacia lentiscus L. NEW PHYTOLOGIST, 170, 779-794.
15.
15. M.
M. TATTINI,
TATTINI, L.
L. GUIDI,
GUIDI, L.
L. MORASSI-BONZI,
MORASSI-BONZI, P.
P. PINELLI,
PINELLI, D.
D. REMORINI,
REMORINI, E.
E. DEGL’INNOCENTI,
DEGL’INNOCENTI, C.
C. GIORDANO,
GIORDANO, R.
R. MASSAI,
MASSAI, G.
G. AGATI
AGATI
(2005).
(2005). On
On the
the role
role of
of flavonoids
flavonoids in
in the
the integrated
integrated mechanisms
mechanisms of
of response
response of
of Ligustrum
Ligustrum vulgare
vulgare and
and Phillyrea
Phillyrea latifolia
latifolia to
to high
high solar
solar
radiation.
radiation. NEW
NEW PHYTOLOGIST
PHYTOLOGIST 167,
167, 457-470.
457-470.

R.Pini, G.Agati, P.Matteini, F.Ratto, F.Rossi, D.Fedele

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