OIE Reference Laboratory Reports Activities Activities in 2015
Transcription
OIE Reference Laboratory Reports Activities Activities in 2015
OIE Reference Laboratory Reports Activities Activities in 2015 This report has been submitted : 2016-02-01 18:04:09 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Q fever Address of laboratory: Anses (Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail), Laboratoire de Sophia-Antipolis, Unité Fièvre Q Animale, 105, route des Chappes, 06410 BIOT, FRANCE Tel.: +33-4 92.94.37.00 Fax: +33-4 92.94.37.01 E-mail address: [email protected] Website: https://www.anses.fr/fr/content/laboratoire-de-sophia-antipolis Name (including Title) of Head of Laboratory (Responsible Official): Richard Thiéry Name (including Title and Position) of OIE Reference Expert: Elodie Rousset Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities, 2015 1 Q fever - Elodie Rousset - france ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Yes Diagnostic Test Indicated in OIE Manual (Yes/No) Indirect diagnostic tests Total number of test performed last year Nationally Internationally ELISA sur sérum caprin, ovin, bovin Oui (en noir) 11802 0 ELISA sur sérum multi-espece (mise Non 415 0 Nationally Internationally Direct diagnostic tests PCR Temps Reel Quantitative Oui (en noir) 1473 6 Génotypage MLVA Non 41 0 PCR / MLVA (17 marqueurs) Non 587 0 Isolement (souris, culture cellulaire) Oui (en noir) 6 0 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? No 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? Yes 2 OIE Reference Laboratory Reports Activities, 2015 Q fever - Elodie Rousset - france Type of reagent available Bactéries purifiées inactivées dosées (Nine Mile Standard ADN génomique dosé (Nine Mile Sérum calibrant Sérum étalon Related diagnostic test PCR PCR, Génotypage ELISA (sérologie) FC Produced/ provide Produit Produit Produit Produit Amount supplied nationally (ml, mg) 14 0.7 27.2 2,5 Amount supplied internationally (ml, mg) 1 0.1 2.8 0 No. of recipient OIE Member Countries Region of recipients 2 Africa Americas Asia and Pacific Europe Middle East 2 Africa Americas Asia and Pacific Europe Middle East 4 Africa Americas Asia and Pacific Europe Middle East 1 Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? No 5. Did your laboratory supply vaccines to OIE Member Countries? No ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? No OIE Reference Laboratory Reports Activities, 2015 3 Q fever - Elodie Rousset - france 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? No ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? No 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Yes Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided AUSTRALIA recommandation de kits ELISA pour porcins explications par e-mail LATVIA support pour la mise en place d'une PCR, recommandations pour recherche sur lait, demande de MR échanges par e-mail (supports, notamment tableau avec objectif méthodes du chapitre oie version 2015), discussion sur site, fourniture de MR LATVIA recommandations en sérologie (antigène CFT, CFT et ELISA, des contoles, une validation) renseignements fournis avec proposition du MR calibrant Elisa, et proposition d'un essai d'aptitude bilateral TUNISIA A la recherche d'un témoin positif de PCR ciblant Coxiella burnetii afin de réaliser des PCR proposition des MR ADN et Bact pour PCR, renseignements sur les certificats, articles transmis pour présenter les 2 méthodes commerciales validées AUSTRIA demande de MR et de participation à une EILA pour sérologie et conseils proposition des MR, explication recommandation ELISA et FC, et proposition d'un essai d'aptitude bilateral ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? No 4 OIE Reference Laboratory Reports Activities, 2015 Q fever - Elodie Rousset - france ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? No 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? No 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 9 Duron Olivier, Noël Valérie, D. McCoy Karen, Bonazzi Matteo, Sidi-Boumedine Karim, Morel Olivier, Vavre Fabrice, Zenner Lionel, Jourdain Elsa, Durand Patrick, Arnathau Céline, Renaud François, Trape Jean-François, Biguezoton Abel S., Cremaschi Julie, Dietrich Muriel, Léger Elsa, Appelgren Anaïs, Dupraz Marlène, Gómez-Díaz Elena, Diatta Georges, Dayo Guiguigbaza-Kossigan, Adakal Hassane, Zoungrana Sébastien, Vial Laurence, Chevillon Christine. 2015. The recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the Q fever pathogen, Coxiella burnetii. PLOS Pathogens. 11(5): e1004892. Duron Olivier, Sidi-Boumedine Karim, Rousset Elodie, Moutailler Sara, Jourdain Elsa. 2015. The importance of ticks in Q fever transmission: what has (and has not) been demonstrated. Trends in Parasitology. 31(11):536-522. Joulié A., Laroucau K., Bailly X., Prigent M., Gasqui P., Lepetitcolin E., Blanchard B., Rousset E., Sidi-Boumédine K., Jourdain E. 2015. Circulation of Coxiella burnetii in a naturally infected flock of dairy sheep: shedding dynamics, environmental contamination, and genotype diversity. Appl Environ Microbiol 81(20):7253-60. doi: 10.1128/AEM.02180-15. Jourdain Elsa, Duron Olivier, Barry Séverine, González-Acuña Daniel, Sidi-Boumedine Karim. 2015. Molecular methods routinely used to detect Coxiella burnetii in ticks cross-react with Coxiella-like bacteria. Infection Ecology and Epidemiology. 5: 29230. Pimenta L., Alegria N., Anastácio S., Sidi-Boumedine K., da Silva G., Rabiço Â., Simões J. 2015. Prevalence of Coxiella burnetii antibodies in Portuguese dairy cattle herds. Tropical Animal Health Production. 47(1):227-30. Prigent Myriam, Rousset Elodie, Yang Elise, Thiery Richard, Sidi-Boumedine Karim. 2015. Validation study for using lab-on-chip technology for Coxiella burnetii multi-locus-VNTR-analysis (MLVA) typing: application for studying genotypic diversity of strains from domestic ruminants in France. Microbes and Infection. 17:782-788 http://dx.doi.org/10.1016/j.micinf.2015.09.026 Rosières Xavier, Rautureau Séverine, Rousset Elodie, Klotz Stéphane, Boulogne Ophélie, Deltour Benjamin. 2015. Cas humains groupés de fièvre Q dans la région Provence-Alpes-Côte d’Azur (Clustered human cases of Q fever in the PACA region). Bulletin Epidémiologique de l'ANSES et la DGAl. 69 :8-10. Sidi-Boumedine Karim, Duquesne Véronique, Prigent Myriam, Yang Elise, Joulié Aurélien, Thiéry Richard, Rousset Elodie. Impact of IS1111 insertion on the MLVA genotyping of C. burnetii. Microbes and Infection. 17(11-12):789-94. doi: 10.1016/j.micinf.2015.08.009. Sidi-Boumedine Karim, Adam Gilbert, Angen Øysten, Aspán Anna, Bossers Alex, Roest Hendrik-Jan, Prigent Myriam, Thiéry Richard, Rousset Elodie. 2015. Whole genome PCR scanning (WGPS) of C. burnetii strains from ruminants. Microbes and Infection. 17(11-12):772-5. doi: 10.1016/j.micinf.2015.08.003. b) International conferences: 7 Communications affichées Joulié Aurélien, Laroucau Karine, Bailly Xavier, Prigent Myriam, Gasqui Patrick, Lepetitcolin Elisabeth, Blanchard Béatrice, Rousset Elodie, Sidi-Boumédine Karim, Jourdain Elsa. Coxiella burnetii circulation in a naturally infected OIE Reference Laboratory Reports Activities, 2015 5 Q fever - Elodie Rousset - france dairy sheep flock: individual shedding, environmental contamination and strain diversity. ESCCAR International Congress on Rickettsia and other Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015. Jourdain Elsa, Duron Olivier, Séverine Barry, Daniel González-Acuña, Sidi-Boumedine Karim. Do genetic markers routinely used to detect Coxiella burnetii in ticks cross-react with Coxiella-like bacteria? ESCCAR International Congress on Rickettsia and other Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015. Pellerin J-L, Alsaleh A., Rodolakis A., Rousset E., Dubreil L., Bruyas J-F., Fieni F.. Risk of transmission of Coxiella burnetii during the caprine in-vitro produced embryo transfer. Xth International Congress of Veterinary Virology ESVV2015 and 9th Annual Meeting of EPIZONE, Montpellier. 3 September. 2015. Prigent Myriam, Rousset Elodie, Yang Elise, Thiery Richard, Sidi-Boumedine Karim. Validation study for using labon-chip technology for Coxiella burnetii multi-locus-vntr-analysis (mlva) typing. Application for studying genotypic diversity of domestic ruminants’ strains in France. ESCCAR International Congress on Rickettsia and other Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015. Rousset Elodie, Dufour Philippe, Hans Aimeric, Gaudaire Delphine, Madeline Anthony, Lecollinet Sylvie, Leblond Agnes, Thiéry Richard, Jourdain Elsa, Gasqui Patrick. Probabilistic model approach for evaluation of a multispecies serological method for Q fever. ESCCAR International Congress on Rickettsia and other Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015. Sidi-Boumedine Karim, Duquesne Véronique, Prigent Myriam, Yang Elise, Joulié Aurélien, Thiéry Richard, Rousset Elodie. Impact of IS1111 insertion on the MLVA genotyping of C. burnetii. ESCCAR International Congress on Rickettsia and other Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015. Sidi-Boumedine Karim, Adam Gilbert, Angen Øysten, Aspán Anna, Bossers Alex, Roest Hendrik-Jan, Prigent Myriam, Thiéry Richard, Rousset Elodie. Whole genome PCR scanning (WGPS) of C. burnetii strains from ruminants. ESCCAR International Congress on Rickettsia and other Intracellular bacteria, Lausanne, Switzerland, June 13-16, 2015. c) National conferences: 7 Communication orale et acte de congrès Joulié A., Laroucau K., Bailly X., Prigent M., Gasqui P., Le Petit Colin E., Blanchard B., Rousset E., Sidi-Boumedine K., Jourdain E. Circulation de Coxiella burnetii dans un troupeau ovin lait naturellement infecté: dynamiques d’excrétion, contaminations environnementales et diversité génotypique. Journées 3R, Paris, 2-3 Décembre, 2015. Communication orale Anastácio S, Pimenta L, Simões J, Alegria N, Rabiço A, Sidi-Boumedine K, da Silva GJ. Coxiella burnetii is present in milk from dairy cattle herds in the Northwest Portugal. V Congresso Luso Brasileiro de Patologia Experimental XV International Symposium on Experimental Techniques Joint Meeting. Coimbre, Portugal, 3-5 December 2015. Joulié A., Sidi-Boumedine K., Rousset E., Leblond Agnès, Jourdain E. Epidémiologie moléculaire de Coxiella burnetii, agent étiologique de la fièvre Q, chez les ruminants domestiques en France. Ecole Vétérinaire, Equipe EPIDEC, Toulouse, 5 Octobre, 2015. Joulié A., Sidi-Boumedine K., Rousset E., Leblond Agnès, Jourdain E. Diversité génotypique de Coxiella burnetii, agent étiologique de la fièvre Q, chez les ruminants domestiques en France. Journée de clôture du dispositif pilote, Paris, 1er Décembre, 2015. Jourdain E, Carrié P., Barry S., Joulié A., Rousset E, Gasqui P., deCrémoux R., Sala C, Gache K., Calvas D. Détection de Coxiella burnetii, agent étiologique de la fièvre Q, dans l’environnement. Journée de clôture du dispositif pilote, Paris, 1er Décembre, 2015. Rousset E., Dufour P., Sala C., Jourdain E. Analyse comparative des 3 kits ELISA pour la sérologie de la fièvre Q. Journée de clôture du dispositif pilote, Paris, 1er Décembre, 2015. Sidi-Boumedine K. The recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the Q fever pathogen, Coxiella burnetii. Journée Scientifique des Laboratoires, Anses, Maisons-Alfort, 18 Novembre, 2015. 6 OIE Reference Laboratory Reports Activities, 2015 Q fever - Elodie Rousset - france Communications affichées Gache Kristel et Groupe de suivi ESA 3-3 Thématique fièvre Q. Fièvre Q : dispositif pilote. Journée annuelle de la plateforme d’Epidémiosurveillance Animale. Paris, 16 juin 2015. Hosteing Soline et Groupe de suivi ESA 3-1 Thématique Avortements des ruminants. Déclaration des avortements : surveillance événementielle de la brucellose. Journée annuelle de la plateforme d’Epidémiosurveillance Animale. Paris, 16 juin 2015. d) Other: (Provide website address or link to appropriate information) 2 Rapport d’essai interlaboratoires d’aptitude : Sérologie fièvre Q sur sérum par ELISA, Session 2015. Rousset, E. et P. Dufour (versions française et anglaise). Rapport Final, 13 avril 2015. 23 pages. Rapport d’analyse quantitative des données issues de l’EILA sérologie fièvre Q sur serum, session 2015. Rousset, E. et P. Dufour (versions française et anglaise). 18 mai 2015. 30 pages. ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? No ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Yes Quality management system adopted Certificate scan (PDF, JPG, PNG format) ISO 17025 Attestation Cofrac 2015 2017.pdf 16. Is your laboratory accredited by an international accreditation body? Yes Test for which your laboratory is accredited Accreditation body ELISA sur sérum COFRAC 17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? OIE Reference Laboratory Reports Activities, 2015 7 Q fever - Elodie Rousset - france Yes (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? No 19. Did your laboratory participate in scientific meetings on behalf of the OIE? No ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? Not applicable (Only OIE Reference Lab. designated for disease) 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Not applicable (Only OIE Reference Lab. designated for disease) 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Not applicable (Only OIE Reference Lab. designated for disease) ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Yes 8 OIE Reference Laboratory Reports Activities, 2015 Q fever - Elodie Rousset - france Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3 Purpose for inter-laboratory test comparisons1 Aptitude à obtenir un résultat sérologique correct par ELISA sur sérums No. participating laboratories 87 Region(s) of participating OIE Member Countries Africa Americas Asia and Pacific Europe Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? No 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities, 2015 9