CV ()

Alice Lebreton Mansuy
Group leader in cellular microbiology,
INRA Research Scientist
Group Bacterial Infection & RNA Destiny
Institute of Biology of École normale supérieure (IBENS)
UMR CNRS 8197, Inserm 1024
46, rue d’Ulm
F – 75 005 Paris
Tel : +33 1 44 32 34 62
e-mail :
http://www.biologie.ens.fr/ida
Nationality : French, Swiss
Languages : French native speaker,
fluent English, school-level Spanish
http://alicelebreton.free.fr
Scientific career
Since 2015
2008-2014
2006-2008
2002-2006
2000
1999
Group leader at the Institute of Biology of École normale supérieure, Paris.
Funding from FRM, ATIP-Avenir, Mairie de Paris, MemoLife.
Regulation of host gene expression in response to infection by intracellular bacteria.
INRA Research scientist (CR1), UIBC (P. Cossart), Pasteur Institute, Paris.
Chromatin subversion & regulation of host gene expression by Listeria monocytogenes.
Post-doctoral fellow, Centre de Génétique Moléculaire (B. Séraphin), CNRS, Gif-sur-Yvette.
Grant from the French National Research Agency – ANR.
RNA exosome activity and nuclear RNA quality control in eukaryotes.
Graduate student, GIM (A. Jacquier), Pasteur Institute, Paris.
Grants from the French Research Ministry and from ARC Foundation for Cancer Research.
Dynamics of ribosome biogenesis in the yeast S. cerevisiae.
Intern, Microbiology Institute (Th. Leisinger), ETH Zürich.
Grant from the Erasmus exchange program.
Transformation of isopropylamine to L-alaninol by Pseudomonas.
Summer intern, La Pitié-Salpêtrière hospital, Paris.
Histology of a murine model for Parkinson Disease.
Education
2015
2010
2002-2006
1998-2002
Habilitation to supervise research (HDR).
Licence for experimentation on mammalian organisms (Nantes Veterinary School).
PhD in Microbiology-Virology, Paris 7 University.
Undergraduate studies in Biochemistry and Cell Biology at École normale supérieure de Cachan :
– 2nd year of Master’s degree in Microbiology-Virology, Paris 7 University/Pasteur Institute,
– Agrégation (French degree for high-school teaching) in Biochemistry – Biological Engineering,
– Bachelor and 1st year of Master’s degree in Biochemistry and Cell Biology, Paris 7 University.
Supervision
Since 2015
2003-2013
Head of a research group at IBENS.
Supervision of three post-doctoral fellows, six students (four Master’s degree and two undergraduates),
a bio-informatician engineer and a technician. Co-supervision of a PhD student. Regular hosting of
undergraduate students for theoretical or practical mini-projects.
Co-supervision of a research engineer and a bio-informatician. Supervision of an assistant-engineer and three
Bachelor’s degree summer students.
Teaching
Since 2015
Since 2014
Since 2011
2005, 2006
2002-2005
Co-organisation of the Microbial Systems course, IMaLiS, École normale supérieure (Master’s degree).
Organisation of the Molecular Cell Biology course for non-biologist students at ENS (Bachelor’s degree).
Lecturer at ENS Cachan for the Cell Biology of Host-Invasive Bacteria Interactions classes (Master’s degree).
Occasional lecturer for Pasteur Institute course of General Microbiology (Master’s degree).
Teaching assistant in Immunology (Bachelor’s degree), Paris 7 University.
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Alice Lebreton Mansuy
CV 2017
Expertise
Keynote speaker for Listeria monocytogenes symposium (VetSuisse, Bern, 2014). Invited speaker for RNA & Infection
(Würzburg, 2016). Member in three PhD thesis committees. Jury member for PhD and Master’s degree thesis defences. Ad
hoc expert for grant proposals and peer-reviewer for scientific journals.
Since 2017
Member of the scientific council of the Microbiology department of INRA.
Since 2014
Jury member for Complexité du Vivant doctoral school (Paris 6 University)
2013-2014
Member of the Pasteur Institute committee for ethics in animal experimentation.
Organization of scientific events and associative activities
Broad audience: Conferences for ENS open night (2016) and for high-school students (2014) on cell piracy by Listeria
monocytogenes. Hosting of secondary school students for laboratory discovery weeks.
Secretary and board member of the StaPa association (Pasteur Institute young researchers) (2004-2005). Co-organizer of
scientific seminars and of the Pasteur Institute young researchers meeting. Co-organizer of three department and team
retreats.
Publications
Research articles
1. Lakisic G, Lebreton A*, Pourpre R*, Wendling O, Libertini E, Radford EJ, Le Guillou M, Champy MF, WattenhoferDonzé M, Soubigou G, Ait-Si-Ali S, Feunteun J, Sorg T, Coppée JY, Ferguson-Smith AC, Cossart P & Bierne H. Role
of the BAHD1 chromatin-repressive complex in placental development and regulation of steroid metabolism. PLoS
Genet. (2016) 12(3):e1005898.
2. Libertini E, Lebreton A, Lakisic G, Dillies M-A, Beck S, Coppée J-Y, Cossart P & Bierne H. Overexpression of the
heterochromatinization factor BAHD1 in HEK293 cells differentially reshapes the DNA methylome on autosomes and
X chromosome. Front Genet. (2015) 6:339.
3. Lebreton A*, Job V*, Ragon M, Le Monnier A, Dessen A, Cossart P & Bierne H. Structural basis for the inhibition of
the chromatin repressor BAHD1 by the bacterial nucleomodulin LntA. mBio (2014) 5(1):e00775-13.
4. Archambaud C, Sismeiro O, Toedling J, Soubigou G, Bécavin C, Lechat P, Lebreton A, Ciaudo C & Cossart P. The
intestinal microbiota interferes with the microRNA response upon oral Listeria infection. mBio (2013) 4(6):e00707-13.
5. Gudipati RK, Xu Z, Lebreton A, Séraphin B, Steinmetz LM, Jacquier A & Libri D. Extensive degradation of RNA
precursors by the exosome in wild type cells. Mol Cell (2012) 48(3):409-21.
6. Bierne H, Travier L, Mahlakõiv T, Tailleux L, Subtil A, Lebreton A, Paliwal A, Gicquel B, Staeheli P, Lecuit M &
Cossart P. Activation of type III interferon genes by pathogenic bacteria in infected epithelial cells and mouse placenta.
PLoS One (2012) 7(6):e39080.
7. Lebreton A, Lakisic G, Job V, Fritsch L, Tham TN, Camejo A, Matteï P-J, Regnault B, Nahori M-A, Cabanes D,
Gautreau, A, Ait-Si-Ali S, Dessen A, Cossart P & Bierne H. A bacterial protein targets the BAHD1 chromatin complex
to stimulate type III interferon response. Science (2011) 331(6022):1319-21.
8. Lebreton A*, Rafal T*, Dziembowski A & Séraphin B. Endonucleolytic RNA cleavage by a eukaryotic exosome. Nature
(2008) 456(7224):993-7.
9. Lebreton A, Rousselle J-C, Lenormand P, Namane A, Jacquier A, Fromont-Racine M & Saveanu C. 60S ribosomal
subunit assembly dynamics defined by semi-quantitative mass spectrometry of purified complexes. Nucleic Acids Res.
(2008) 36(15):4988-4999.
10. Pertschy B, Saveanu C, Zisser G, Lebreton A, Tengg M, Jacquier A, Liebminger E, Nobis B, Kappel L, van der Klei I,
Högenauer G, Fromont-Racine M & Bergler H. Cytoplasmic recycling of 60S pre-ribosomal factors depends on the
AAA-protein Drg1. Mol Cell Biol. (2007) 27(19):6581-92.
11. Lebreton A, Saveanu C, Decourty L, Jacquier A & Fromont-Racine M. Nsa2, an unstable, conserved factor required for
the maturation of 27SB pre-rRNAs. J Biol Chem. (2006) 281(37):27099-108.
12. Lebreton A, Saveanu C, Decourty L, Rain J-C, Jacquier A & Fromont-Racine M. A functional network involved in the
recycling of nucleo-cytoplasmic pre-60S factors. J Cell Biol. (2006) 173(3):349-60.
13. Saveanu C, Namane A, Gleizes P-E, Lebreton A, Rousselle J-C, Noaillac-Depeyre J, Gas N, Jacquier A & FromontRacine M. Sequencial protein association with nascent 60S ribosomal particles. Mol Cell Biol. (2003) 23(13):4449-60.
14. De Azevedo Wäsch SI, van der Ploeg JR, Maire T, Lebreton A, Kiener A & Leisinger T. Transformation of
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Alice Lebreton Mansuy
CV 2017
Isopropylamine to L-Alaninol by Pseudomonas sp. Strain KIE171 Involves N-Glutamylated intermediates. Appl Environ
Microbiol. (2002) 68(5):2368-75.
* Equal contribution.
Reviews and synthesis
15. Lebreton A*, Stavru F*, Brisse S & Cossart P. 1926-2016: 90 years of listeriology. Microbes Infect. (2016) 18(12):71123.
16. Duval M, Cossart P‡ & Lebreton A‡. Mammalian microRNAs and long noncoding RNAs in the host-bacterial pathogen
crosstalk. Semin Cell Dev Biol. (2016).
17. Lebreton A‡ & Cossart P‡. RNA- and protein-mediated control of Listeria monocytogenes virulence gene expression. RNA
Biol. (2016).
18. Lebreton A, Stavru F & Cossart P. Organelle targeting during bacterial infections: Lessons from Listeria. Trends Cell
Biol. (2015) 25(6):330-338.
19. Cossart P‡ & Lebreton A‡. A trip in the “New Microbiology” with the bacterial pathogen Listeria monocytogenes. FEBS
Lett. (2014) 588(15):2437-2445.
20. Lebreton A, Cossart P & Bierne H. Bacteria tune interferon responses by playing with chromatin. Virulence (2012)
3(1):1319-21.
21. Lebreton A & Séraphin B. Exosome-mediated quality control: substrate recruitment and molecular activity. Biochim
Biophys Acta (2008) 1779(9):558-65.
22. Lebreton A. Dynamique des facteurs pré-ribosomiques au cours de la biogenèse de la grande sous-unité ribosomique
chez S. cerevisiae. (2006) Thesis manuscript.
‡ Corresponding authors.
Broad audience publications
23. Lebreton A, Bierne H & Cossart, P. Les multiples stratégies de Listeria. Pour la Science (2012) (412):42-9.
24. Lebreton A. La mise en évidence du bacille de la peste par A. Yersin, Hong-Kong 1894. bibnum (2009).
25. Lebreton A. La génétique et les lois de l’hérédité. Encyclopédie Clartés (2004) 6020:1-22.
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