Digestion de protéines en gel avec OMX-S pour MS
Transcription
Digestion de protéines en gel avec OMX-S pour MS
Digestion de protéines en gel avec OMX-S pour MS - 4 étapes : du prélèvement d’un spot à l’échantillon prêt pour l’analyse en spectrométrie de masse OMX-S Kit pro Contient: OMX-S basic Contient: CM0330, 12 unités C12010, per sample 12 OMX-S® devices + Buffers for tryptic digestion (incl.modified trypsin) OMX-S MS analysis Contient: CM0340, 30 unités 12 OMX-S® devices + Sampler + Pipette tips OMX-S Kit basic Contient: 50 μl 10 ml 1.0 ml 1.0 ml 1.0 ml 3x1.0 ml 30 OMX-S® devices OMX-S pro Contient: CA0660, 12 unités 12 OMX-S® devices 12 capped Peptide Sampler 30 Pipette tips Modified Trypsin (5 μg) Digestion buffer (pink cap) Silver destaining solution 1 (yellow cap) Silver destaining solution 2 (white cap) Reduction reagent (blue cap) Alkylation reagent (black cap) CM0350, per sample 12 OMX-S® devices + in-gel digestion + MS-analysis (custom) Interbiotec - BioSciences innovation – proposes a complete range of products : Staining of protein gels The best of stains, whatever you work with coomassie, silver or by fluorescence: Description cat.# CooBlue FX Instant Protein Stain UPG4562A, 500ml Quick staining, safe/no MetOH or acid, 20ng sensitivity UPG4562B, 4.5L ProSave™ Protein Gel stain BP7121, kit Ultrafast staining (7min!), reversible and compatible/any appl. SilverBullit T08860, 1 kit (qsp 2.5L) Very sensitive and convenient, compatible with MS LavaPurple Protein Gel AND Blot stain Fluorescent staining, the most sensitive (50pg); identifies more proteins by LC/MS, very economic (9€/gel)! 67433A, kit 20 minigels 67433B, kit 100 minigels 67433C, kit 20 minigels Prices and technical sheets on-line More information Preparative Electrophoresis: OFF Gel Ideal to fractionnate proteins according their pI from complex samples Resolution 0.1-06 pH– load µg to mg Increase considerably the number of proteins identified by LC/MS More information 3100 OFFGEL system CF3900-G3100AA , 1u Prices and technical sheets on-line Detect proteins and peptides in solutions with the right tool ! Product Fluorescence method:LavaPep peptide & proteins assay The most sensitive assay, down 160pg sensitivity. Ideal for peptides assay in biochemistry works (purification, labeling, before MS analysis...) LavaDigest™ Protease Monitoring Kit a simple real time monitoring of protein digestions before peptides analysis BCA method: BC Assay protein determination kit the reference protein assay in R&D lab, overcoming Lowry & Bradford method MicroBC Assay protein determination kit the version of BC Assay for highest sensitivity, down 1pg protein/ml Bradford method: Coo Assay protein determination reagent An improved Bradford assay, down 1pg protein/ml Compatible Protein assays in solution Protein gels electrophoresis stains Related products/documents Products HighLights Overview Information inquire Reply by Fax : +33 (0) 4 70 03 82 60 or email at [email protected] □ I wish to recieve the complete documentation about: _______________________________________________________ _________________________________________________________________________________________________________________ Name: _____________________ 2nd name:__________________ Position:____________________ Company/Institute: ___________________________ Adress: _________________________________________________________________ Zip code: _________ Town: Service, Lab:_____________________________ ___________________ Tel____________________ Fax___________________ Email: ____________________________________