Digestion de protéines en gel avec OMX-S pour MS

Transcription

Digestion de protéines en gel avec OMX-S pour MS
Digestion de protéines en gel avec OMX-S pour MS
- 4 étapes : du prélèvement d’un spot à l’échantillon prêt pour l’analyse en spectrométrie de masse
OMX-S Kit pro
Contient:
OMX-S basic
Contient:
CM0330, 12 unités
C12010, per sample
12 OMX-S® devices + Buffers for tryptic digestion (incl.modified trypsin)
OMX-S MS analysis
Contient:
CM0340, 30 unités
12 OMX-S® devices + Sampler + Pipette tips
OMX-S Kit basic
Contient:
50 μl
10 ml
1.0 ml
1.0 ml
1.0 ml
3x1.0 ml
30 OMX-S® devices
OMX-S pro
Contient:
CA0660, 12 unités
12 OMX-S® devices
12 capped Peptide Sampler
30 Pipette tips
Modified Trypsin (5 μg)
Digestion buffer (pink cap)
Silver destaining solution 1 (yellow cap)
Silver destaining solution 2 (white cap)
Reduction reagent (blue cap)
Alkylation reagent (black cap)
CM0350, per sample
12 OMX-S® devices + in-gel digestion + MS-analysis (custom)
Interbiotec - BioSciences innovation – proposes a complete range of products :
Staining of protein gels
The best of stains, whatever you work with coomassie, silver or by fluorescence:
Description
cat.#
CooBlue FX Instant Protein Stain
UPG4562A, 500ml
Quick staining, safe/no MetOH or acid, 20ng sensitivity
UPG4562B, 4.5L
ProSave™ Protein Gel stain
BP7121, kit
Ultrafast staining (7min!), reversible and compatible/any appl.
SilverBullit
T08860, 1 kit (qsp 2.5L)
Very sensitive and convenient, compatible with MS
LavaPurple Protein Gel AND Blot stain
Fluorescent staining, the most sensitive (50pg); identifies more
proteins by LC/MS, very economic (9€/gel)!
67433A, kit 20 minigels
67433B, kit 100 minigels
67433C, kit 20 minigels
Prices and technical
sheets on-line
More information
Preparative Electrophoresis: OFF Gel
Ideal to fractionnate proteins according their pI from complex samples
Resolution 0.1-06 pH– load µg to mg
Increase considerably the number of proteins identified by LC/MS
More information
3100 OFFGEL system
CF3900-G3100AA , 1u
Prices and technical sheets on-line
Detect proteins and peptides in solutions with the right tool !
Product
Fluorescence method:LavaPep peptide & proteins assay
The most sensitive assay, down 160pg sensitivity. Ideal for peptides assay in
biochemistry works (purification, labeling, before MS analysis...)
LavaDigest™ Protease Monitoring Kit
a simple real time monitoring of protein digestions before peptides analysis
BCA method: BC Assay protein determination kit
the reference protein assay in R&D lab, overcoming Lowry & Bradford method
MicroBC Assay protein determination kit
the version of BC Assay for highest sensitivity, down 1pg protein/ml
Bradford method: Coo Assay protein determination reagent
An improved Bradford assay, down 1pg protein/ml
Compatible Protein assays in solution
Protein gels electrophoresis stains
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