ASSELINE Ulysse-CURRICULUM VITAE Asseline ulysse Education
Transcription
ASSELINE Ulysse-CURRICULUM VITAE Asseline ulysse Education
ASSELINE Ulysse-CURRICULUM VITAE Asseline ulysse Directrice de Recherches Professional address : Centre de Biophysique Moléculaire UPR- 4301- CNRS. Rue Charles Sadron - 45071 - Orléans Cedex 2 Tel : (02) 38 25 55 97 E-mail: ulysse.asseline@cnrs-orleans Education 1983 Doctorat d’Etat ès-Sciences Physiques. Université d’Orléans. Titre : Synthèse et études physicochimiques d’oligonucléotides modifies et non modifiés. Directeur de thèse : Pr C. Hélène. Boursière de la Ligue Nationale Française Contre le Cancer. 1980 Doctorat de 3e Cycle. Université d’Orléans. Titre: Contribution à l’étude des dérivés monosubstitués des acides du phosphore tétracoordiné : Application à la synthèse des nucléotides. Directeur de thèse : Dr P. Chabrier. Allocataire DGRST. Academic positions and stays 1985 CNRS researcher (CR1) - Centre de Biophysique Moléculaire UPR - 4301 -CNRS Orléans. Team: Oligonucleotides 01-02-1991-31-01-1992. Laboratory of Molecular Biology- Medical Research Council Cambridge (UK). Contribution to Tat-TAR interaction studies of VIH 1 (Team: Dr M. Gait). 1996-present. Directrice de Recherches CNRS (Senior CNRS researcher). Centre de Biophysique Moléculaire UPR-4301-CNRS Orléans 1997-2011. Team leader. Oligonucléotides Modifiés: chimie et applications 2011-2016. Team: Optical sensors and oligonucleotidic probes for bioanalyses and imaging (leaders: Dr. S. Petoud then Pr J. Hamacek). Research activities Research activities focus on the development of novel oligonucleotides with the purpose to obtain tools for basic research, in vitro diagnostics, bio-imaging, as well as potential therapeutic agents. Thus, depending of the intended applications, the oligonucleotides should possess several of the following properties: to be able to form specific and stable complexes with their targets and either to induce irreversible modifications of the targets (by cleavage or cross-linking reactions) or to produce a modified signal, in the presence of the target, that can be used as a proof of hybridization. Furthermore, for the applications in cultured cells and in vivo the oligonucleotides must be resistant to nucleases and capable of reaching their targets inside the cells. In order to provide the oligonucleotides with the required properties for those applications, we chemically modify the different structural units of their backbones (nucleic bases, internucleotidic linkages, sugar moieties). These modified oligonucleotides are covalently attached to various ligands (intercalating agents, cleavage and cross-linking reagents, peptides, lipophilic compounds, topoisomerase inhibitors, visible and NIR labels ….). The development of conjugates with optimized properties depends on linkage parameters between both entities (positions of attachment on the oligonucleotides and the ligands, length and nature of the linkers). The chemical syntheses of these new oligonucleotide series require the development of new reagents and synthetic methods. These interdisciplinary programs are developed in the framework of collaborative work with biochemists and biologists at CBM as well as with other academic laboratories and private companies. (Financial supports: CEE-BioMed, ANRS, CNRS, ANR, Region Centre, Département du Loiret and private companies). Scientific production 100 publications (including 3 reviews and 11 book chapters) + 2 patents H-index (Web of Science) 29 Teaching 1993-2013 Cours de Chimie des oligonucléotides en DEA puis Master 2 chimie. DEA de chimie et physicochimie des composés d’intérêt biologique (Université d’Orléans, Paris V, Paris XI) de 1993 à 2000. DEA Conception, synthèse, analyse et structure des composés d’intérêt biologique puis en Master 2 COSAMIB (Université d'Orléans) de 2000 à 2013. 2006-2008 Cours de chimie des oligonucléotides en Master 1 de Biochimie (Université d’Orléans). 1980-1982 Travaux pratiques de chimie organique. IUT de Chimie Orléans