Translation 3046

FISUERIES AND MARINE SERVICE
Translation Series No. 3046
Fatty acids in blood serum of calves from birth to weaning
•
by 0. Catarsini, F. De Pasquale, F. Meli, and V..Pantano
Original title: Cil acidi grassi nel siero - di sangue•di vitelli dalla
nascita allo svezzamento
From: Atti della societl italiana delle scienze.vetë , rinarle (Acta
of the Italian.Societv of Veterinary Sciences), 26 : 300-302,
1972
.Translated by the Translation Bureau(M1)
, Multilingual Services'Division
Department of the Secretary of State of. Canada
Department of the Environment.
Fisheries and Marine Service.
Halifax Laboratory
. Halifax, N.S.
1974'
4 pages typescript
q
SECRETARIAT D'ÉTAT
DEPARTMENT OF THE SECRETARY OF STATE
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TRANSLATED FROM - TRADUCTION DE
INTO - EN
I tal iGn
English
AUTHOR - AUTEUR
0. Catarsini et al.
TITLE IN ENGLISH - TITRE ANGLAIS
Fatty acids in the blood serum of calves from birth to weaning
TITLE IN FOREIGN LANGUAGE (TRANSLITERATE FOREIGN CHARACTERS)
TITRE EN LANGUE ETRANGERE ( TRANSCRIRE EN CARACTÈRES ROMAINS)
Gli acidi grassi nel siero di sangue di vitelli dalla nascita allo
svezzamento
REFERENCE IN FOREIGN LANGUAGE (NAME OF BOOK OR PUBLICATION) IN FULL. TRANSLITERATE FOREIGN CHARACTERS.
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Atti de_l1a societ%â. italiana delle scienze veterinarie
REFERENCE IN EtiGLiSH - RéFÉREnCE EN ANGLAIS
Acta of the Italian Society of Veterinary Sciences
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1972
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n-71
2 1 1974
Catarsini, O., De Pasquale, F., Meli, F. and Penton, V:
FATTY ACIDS IN THE BLOOD SERUM OF CALVES FROM BIRTH TO
WEANING
Gas-chromatographic research (Institute of Special Pathology and Veterinary Medicine of the University of Messina)
In five calves of the Frisian breed, the behaviour of the
fatty acids of the blood serum was studied from birth to weaning by
means of gas-chromatography in the gaseous phase.
During the first week of life, the animals were fed by the
mothers; at the beginning of the second week, they were moved to
separate stalls and fed artificially, with a mechanical wet-nurse
regulated in such a way that each calf could suck about 2.5 liters
of milk at each feeding.
At the same time, whole compound feed and
hay of alfalfa were placed at their disposal.
The blood samples (in all, nine were taken for each animal,
at the rate of one a week) were always taken between 9:00 and 10:00
a.m., after the animals had received their first ration of milk.
The fatty acids were extracted from the serum using the method
of DE PASQUALE.
505-200-10-31
7530-21-029-5332
The methyl esters were separated by means of a Carlo
,
_ it-t
f"1
2
Erba gas chromatograph, serial 200, mod. GV, with flame ionization
0)
detectors and a stainless steel double column (2 m x 2 mm inner
The conditions
filled with 20% EAS on silanized chromosorb 60-80 mesh.
of separation were the following: temperature of columns 95 °-155C,
o
increase 5 ° C/mi, initial isotherm 5', temperature of detector 300 C,
2
temperature of injectors 270°C, carrier gas N 2 1.35 kg/c equal to
2
50 ml/min., combustible gas H 0.5 kg/c equal to 11 ml/min., combustible
2
2
gas air 1.5 kg/c equal to 340 ml/min., inlet pressure 100 outlet
pressure 16, bottom of graph scale 2.5 mV, speed of paper 1/5 inch/min.
The gas-chromatographic separation revealed the constant
presence of 32 fatty acids; in some samples, up to 35 fatty acids
were found, due to the appearance of acids not constantly present.
Of
C , C , C , C , C ,
these fatty acids, 20 were identified (C , C
11
12
9
10
6
7' 8
C
C
13'
iso C
C
14'
18'
C
15'
anteiso C
15'
C
16'
iso C
anteiso C
17'
17'
C
C
17'
18,
, iso C20). The unidentified fatty acids are indicated
18
by an X and numbered progressively from 1 to 12.
On the graph, the pds-
C6 ,
ition of the unidentified acids was the following: X 1 precedes•
X2 between C
6
and C
and
X 6 between C
C
and C
C
11
12'
•X
10
9
7'
•X
and C
and X
3
between C
10'
11
•X
7
and X
between C
and C
7
8
12
8'
•X
4
between C
and C
13'
between C
10
•X
12
and C
8
11'
and C
•X
between C
9'
• X5
between
9
16
and anteiso
17 .
In many cases, between C
and C
13'
10
and C
as well as between C
11'
one acid appeared instead of two.
X
1 , X3'
X
4
and X
9'
12
in some
samples, were accompanied by the appearance of one or two unidentifeed
acids
.
•
,
3
Ir.
From the examination of the results, which for the sake of
brevity, we are not reporting in this note, we found that, among the
fatty acids identified, C 7 , C 8 , C 9 and C 10 underwent increasing variations,
In particular,
between the beginning and the end of the experiment.
C 8 descended from a basic mean value of 12.330%
to 1.142% at the 4th
sample and then gradually rose again to 32.836% at the 9th sample.
In the course of the research, the C 11' C 12, C 14' C 15' iso C 16' iso
C 18 and iso C 20 diminished.
were observed for C
11'
C
14'
The fatty acids C
C
20
13
In this group, the most consistent decreases
iso C
18
, iso C
and iso C20 .
14
, anteiso C
15
, C 18 and anteiso
varied considerably, showing increases and decreases, but return-
ing, at the end of the experiment, to values quite close to those of
the 1st sample taken.
C6 , C 16 ,C17 andC18 7.showed inconsistent variations.
Among the unidentified fatty acids, we found a constant
increase in X
4
which rose from a mean percentage value of 1.4% in
the first sample taken, to one of 10.436% in the 9th sample taken.
X
5'
showed a tendency to increase and X6 ,(decrease.
X
12
, of which
the mean percentage value in the first sample taken was 5.20%, rose
to 9.058% in the 3rd sample, after which it gradually decreased, until
it dropped to 0.32 in the 9th sample taken.
These results show that in calves, between birth and weaning,
there is a modification in the percentage of fatty acids of the serum,
which generally consists in an increase in fatty acids with a lower number
of C atoms (particularly C 8 and X4 , this latter appearing between C8
4
and C ) and in a decrease which is more or less marked in those with
9
a larger number of C atoms (in addition to those identified and listed
previously, there is the X
C
17
12
which appears between C 16 and anteiso
).
The results of our research lead us to suppose that the modifications we observed during the first 75 days of the life of the
calves are a physiological phenomenon due either to the type of feed,
or to the progressive development of the rumen which permits
use of foods other than milk even if only partially,
the
In later
researches, we shall attempt to verify this hypothesis.
SUMMARY
RÉSUMÉ — Les acides gras dans le sérum de sang des z7caux de lait après la
naissance jusqu'au sevrage. Récherches par la chromatographie in phase gazeuse Les an. ont étudié, dans un groupe des veaux de lait, par la chromatographie • in phase
gazeuse, les acides gras du sértuu après la naissance jusqu'au sevrage. Pendans les 75
jours (l'observation on a régistré cettes modiphications:
et C17 sont augmentés;
Cu, Cu, Cu, C,,, isoC,, et isoC is sont diminués; Cly, isoC,,, anteisoC u, Cu et anteisoC e
sontreépqugalbin'oservédaitnmrespluo
moins large; C6, C16, C17 et CuL--- sont changés irrégulièrement. Dans les 12 acides gras
ne pas identifiés on a régistré l'augmentation de X, (cet acide, dans les diagramme, se
trouve parmi C, et C9) et la diminution de X u (qui se trouve parci CI, et anteisoC u).
Les aa. pensent que les changements enregistrées soint un fait phisiologique pendant les premièrs 75 jours de vie.
•
SUMMARY — Patty acids in blood serum of calves from their birth to their :yeaning-gas.chromatography researches - The authors have stutied, through gas-chromatography, te behavior of the fatty acids of the serum in a group of calves front their
birth to their weaning. During the 75 days of their observations they have checked the
following modifications: C7, C3, C9 and C 19 have increased; C11, C19.9 C14, CIS, ISOC16, iSOC19
have dirninaiSiled C13, i20C11, anteis.oCu, C.:, and anteisoC u have remained almost 'invariable, although intermediate variations more or lees large had been observed, and
CS, C16, C17 and C a -z-- have changed without any rule. Among the 12 unidentificated.
fatty acids have been observed both a considerable increase of X, (this acid, in the
diagram, appears between C, and CO and a reduction of X12 (it appears between Cu
and anteisoC u).
The authors think that the modificaticns are concerned with a physiological fact
during the first 75 days of life.
Per la biblioarafia si rimanda ai lavoro in e.ttensc