Protein crystallography without crystals: A novel molecular sieve for

Protein crystallography without crystals: A novel molecular sieve for easy and rapid
crystal structure determination of proteins
Initiative: "Experiment!"
Ausschreibung: Explorative Phase
Bewilligung: 08.12.2015
Laufzeit: 1 Jahre 6 Monate
Structure determination by protein crystallography is limited by the ability of proteins to crystallize.
Since many proteins cannot be crystallized, high resolution X-ray structure information is often
missing. The applicant proposes to develop a novel and rapid approach for structure determination
by making use of a molecular framework for protein crystallization. This will eliminate the need
to crystallize proteins in the conventional way but still would provide high-resolution structural
information. The molecular framework will act like a molecular sieve, which will trap proteins. The
structure of the protein can then be easily determined in complex with the sieve. If successful, this
will open a universal, easy, fast and low sample consuming approach that will eliminate the biggest
hurdle of protein crystallography.
Projektbeteiligte
Dr. Masato Akutsu
Universität Frankfurt am Main
Buchmann Institute for Molecular Life Sciences
Frankfurt am Main
Es werden die Institutionen genannt, an denen das Vorhaben durchgeführt wurde, und nicht die aktuelle
Adresse.
16.01.2017
1