MiDas - Gilson

Transcription

MiDas - Gilson

MiDas™
User’s Guide
Gilson, Inc. | PO Box 620027 | Middleton, WI 53562-0027 | Tel: 608-836-1551 OR 800-445-7661 | Fax: 608-831-4451
Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 Villiers-le-bel, FRANCE
www.gilson.com | [email protected] | [email protected] | [email protected]
LT319231-01
Table of Contents
Safety
Electronic and Hazard Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Safety Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Chemical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Protective Covers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Stacking Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Sécurité
Symboles Électroniques et de Dangers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Notes de Sécurité . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Risques Chimiques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Précautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Les Capots de Protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Sécurité de l’empilement des appareils . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
1 Introduction
Inspect Shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Splitter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Make Up Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Direct Sampling Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Customer Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Terms and Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
MiDas™ | User’s Guide
Table of Contents
2 Installation
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Front Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Plumbing Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Direct Sampling Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Splitter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Make Up Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Pressure Bypass Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Pressure Relief Valve Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Rear Panel Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
USB Cable Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Power Cord Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
3 Operation
Prime the Make Up Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Set Make Up Pump Flow Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Set Splitter Split Ratio . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
4 Maintenance
Maintenance Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Cleaning and Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Direct Sampling Valve Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Position Valve for Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Remove Valve Driver . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Install Replacement Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Splitter Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Disassemble Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Reassemble Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Make Up Pump Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Backflush the Pistons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Switch Off the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Replace the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Clean the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Safe State Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Table of Contents | MiDas™
Troubleshooting Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Repair and Return Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Before Calling Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Warranty Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Warranty for Non-Gilson Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Non-Warranty Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Return Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Unit End-of-Life . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
A Replacement Parts
B Chemical Compatibility Chart
C Appropriate Delivery Rates
Table of Contents
5 Troubleshooting
Safety
Read this chapter before installing and operating the instrument.
Only trained technical personnel in a laboratory environment may use the instrument for non-medical, liquid
handling purposes. For safe and correct use of the instrument, operating and service personnel must follow
all instructions contained in this guide when installing, cleaning, and maintaining the instrument. All safety
precautions must be observed during all phases of operation, service, and repair of the instrument.
Failure to comply with these precautions or with warnings described in the user’s guide violates safety
standards of design, manufacture, and intended use of the instrument. Gilson assumes no liability for
customers failing to comply with these requirements.
The instrument has been certified to safety standards required in Canada, Europe, and the United States.
Refer to the rear panel label on the instrument and the Declaration of Conformity document for the current
standards to which the instrument has been found compliant.
7
Safety
Electronic and Hazard Symbols
The following safety symbols and notices may appear on the instrument or in this document:
Symbol
~
Explanation
Alternating Current
Caution
Attention
Vorsicht
WARNING
Caution, risk of electric shock
Attention, risque de choc électrique
Vorsicht, Elektroschockgefahr
WARNING
Warning, hazardous voltage
Avertissement de tensions dangereuses
Hochspannung Vorsicht
Warning, corrosive chemical
Avertissement de substances corrosives
Achtung, korrosiven Chemikalien
Safety Notices
The following safety notices may appear in this document:
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, may result
in serious injury
CAUTION
CAUTION indicates a potentially hazardous situation which, if not avoided, may result in
minor or moderate injury
NOTICE
NOTICE indicates a potentially hazardous situation which, if not avoided, may result in
equipment damage
Chemical Hazards
Any chemicals used for analysis should be handled according to good laboratory practice. They should also
be stored, used, and disposed of in accordance with the manufacturer’s specifications, as well as local and
national regulations. Potentially hazardous chemicals can be used with the MS detector. Use care when
handling chemicals and wear appropriate personal protective equipment (PPE).
The responsible individual must ensure that personnel are not exposed to hazardous levels of toxic
substances as outlined in the Material Safety Data Sheets (MSDS), or any documentation provided by
local governing bodies such as The Health Protection Agency (UK) or The Occupational Safety and Health
Administration (US).
8
Safety | MiDas™
The following safety precautions concerning the operation of the MS detector and the site in which it is
used must be observed at all times in order to prevent injury and damage to the system and associated
instruments.
Safety
Precautions
Do not use the instrument if there are signs of visible damage.
Do not operate if site conditions are not within specifications.
WARNING
WARNING
Do not override any safety interlock.
Do not attempt to operate the instrument with the covers removed.
Do not attempt to adjust or replace components other than those that have been
described in this user’s guide.
The instrument is not designed to be operated in a potentially explosive atmosphere.
It is the responsibility of the customer to verify the operating environment meets
the requirements of a “non-hazardous area” with regards to potentially explosive
atmosphere.
WARNING
At least two people are required to lift or move the MS detector.
Protective Covers
Unless specifically instructed, do not remove any protective covers. The product is sealed at the factory.
Damage or removal of the warranty seals leads to the loss of liability and warranty entitlements.
Stacking Safety
Allow sufficient spacing around the MiDas™ for proper cooling and for the connection
of mains plug, syringe pump, etc.
CAUTION
The center of gravity for the MiDas™ is situated towards the front of the unit. Ensure
this is observed when positioning or moving the unit. Lifting the unit from the front is
recommended.
9
Sécurité
Merci de lire attentivement cette section avant toute installation ou utilisation de l’instrument.
Cet instrument est exclusivement destiné à être utilisé dans un environnement de laboratoire, par un
personnel qualifié, à des fins de manipulations de liquides non-médicales. Pour une utilisation correcte
et en toute sécurité de l’instrument, il est nécessaire que le personnel qui utilise et réalise la maintenance
de l’instrument, suive les instructions contenues dans ce guide lors de l’installation, du nettoyage et de la
maintenance de l’instrument. Toutes les consignes de sécurité doivent être respectées durant toutes les
phases de fonctionnement, d’entretien ou de réparation de l’instrument.
Le non-respect de ces précautions ou des avertissements spécifiques mentionnés dans ce guide compromet
les normes de sécurité de conception, de fabrication et d’utilisation prévue de l’instrument. Gilson décline
toute responsabilité en cas d’incapacité du client à se conformer à ces exigences.
L’instrument a été certifié conformément aux normes de sécurité en vigueur au Canada, en Europe et aux
Etats-Unis. Merci de vous reporter aux indications mentionnées sur le panneau arrière de l’instrument
ainsi qu’au document de Déclaration de Conformité aux normes pour lesquelles l’instrument a été déclaré
conforme.
11
Sécurité
Symboles Électroniques et de Dangers
Les symboles électroniques et de dangers suivants peuvent apparaitre sur l’instrument:
Symbole
Signification
Alternating current
Courant alternatif
Wechselstrom
~
Caution
Attention
Vorsicht
Caution, risk of electric shock
Attention, risque de choc électrique
Vorsicht, Elektroschockgefahr
WARNING
Warning, hazardous voltage
Avertissement de tensions dangereuses
Hochspannung Vorsicht
Warning, corrosive chemical
Avertissement de substances corrosives
Achtung, korrosiven Chemikalien
Notes de Sécurité
Les notes de sécurité suivantes peuvent apparaitre dans ce document:
AVERTISSEMENT
AVERTISSEMENT: indique une situation potentiellement dangereuse qui, si
elle n’est pas évitée, peut entraîner des blessures graves
ATTENTION
ATTENTION: indique une situation potentiellement dangereuse qui, si elle
n’est pas évitée, peut entraîner des blessures mineures à modérées
AVIS
12
Sécurité | MiDas™
AVIS: indique une situation potentiellement dangereuse qui, si elle n’est pas
évitée, peut entraîner des dégâts matériels
Tous les produits chimiques utilisés pour l’analyse doivent être manipulés conformément aux bonnes
pratiques de laboratoire. Ils doivent être stockés, utilisés et éliminés conformément aux recommandations
du fabricant ainsi qu’à la réglementation locale et nationale. Des produits chimiques potentiellement
dangereux peuvent être utilisés avec le détecteur du spectromètre de masse (détecteur MS). Manipuler ces
produits avec précaution et porter un équipement de protection individuelle adapté (EPI).
Sécurité
Risques Chimiques
Le responsable doit veiller à ce que le personnel ne soit pas exposé à des niveaux dangereux de substances
toxiques comme indiqué dans les fiches de données de sécurité (FDS), ou toute documentation fournie par
les instances locales telles que l’Agence de protection de la santé (Royaume-Uni) ou l’Administration de la
sécurité et de la Santé (US).
Précautions
Les consignes de sécurité concernant le fonctionnement du détecteur MS ainsi que l’endroit où il est
utilisé, doivent être respectées à tout moment, afin d’éviter tout risque de blessure des utilisateurs, ou tout
dommage au système lui-même ou aux instruments associés.
Ne pas utiliser le détecteur MS en cas de détérioration visible.
Ne pas faire fonctionner si le site présente des conditions non conformes aux
spécifications.
WARNING
AVERTISSEMENT
Ne pas désactiver les dispositifs de verrouillage et de sécurité.
Ne pas faire fonctionner le détecteur MS sans les capots.
Ne pas tenter de régler ou de remplacer des éléments autres que ceux décrits
dans le guide de l’utilisateur.
Le détecteur MS n’est pas conçu pour une utilisation en atmosphère
potentiellement explosive. Il est de la responsabilité du client de vérifier
que l’environnement d’exploitation répond aux exigences de ‘’zone non
dangereuse’’ en ce qui concerne le risque d’atmosphère potentiellement
explosive.
AVERTISSEMENT
Un minimum de deux personnes est requis pour soulever ou déplacer le
détecteur MS.
Les Capots de Protection
Sauf mention contraire, ne pas retirer les capots de protection. Le produit est scellé à l’usine. Des scellés de
garantie endommagés ou absents entraînent l’annulation de la responsabilité et des droits de garantie.
13
Sécurité
Sécurité de l’empilement des appareils
Laisser un espace suffisant autour de MiDas™ pour le refroidissement et pour la
connexion de prise secteur, pompe seringue, etc.
ATTENTION
14
Sécurité | MiDas™
Le centre de gravité du MiDas™ est situé vers l’avant de l’appareil. Prenez en compte
lors du positionnement ou pendent le déplacement de l’apparei. Il est recommandée
de soulever l’unité par l’avant.
1 Introduction
Chapter One
This chapter provides information on the following:
●● Inspect Shipment on page 16
●● Inspect Shipment on page 16
●● Customer Service on page 17
●● Terms and Abbreviations on page 17
●● Trademarks on page 17
●● Technical Specifications on page 18
15
Inspect Shipment
Inspect Shipment
Upon receipt of the instrument, inspect the exterior of all shipping cartons. All cartons should arrive
unopened and undamaged. If examination reveals that damage has occurred in shipment, notify the carrier,
and Gilson immediately.
Description
MiDas™ is an accessory for the VERITY® 1900 MS Detector that includes an integrated make up pump, splitter,
and direct sampling valve. The components divert a small portion of flow from the HPLC system to the
MS detector, which allow analytes of high concentration to be sampled, allow the reduction of the flow rate
to a manageable level and allow flow from the HPLC system to be diluted in an ESI-compatible solvent.
Figure 1: MiDas™
Splitter
The function of the splitter is to divert a small portion of a flow so that it can be diluted in the make up pump
solvent. This allows the MiDas™ to accept samples at saturated concentrations and in non-polar solvents that
would be otherwise incompatible with electrospray ionization (ESI).
Make Up Pump
MiDas™ incorporates a make up pump that dispenses from an external reservoir. This reservoir is filled with
an ESI compatible solvent (e.g., water, methanol, acetonitrile, etc.) with the conventional modifiers (such as
formic acid, ammonium acetate, etc.).
NOTE
Care should be taken to ensure that the analyte has moderate solubility in the make up pump
solvent so that precipitation does not occur within MiDas™.
Direct Sampling Valve
MiDas™ features a 2-position, 6-port valve. The recommended operation has a storage loop and syringe
port configured as a loop injector. The valve body is polyetheretherketone (PEEK) chosen for its chemical
compatibility.
NOTE
16
Care should be taken to ensure that the sample does not contain solid particulates that could
damage the moving parts. It is recommended to extract the sample through a filter prior to
injecting into the valve.
Introduction | MiDas™
Gilson, Inc. and its worldwide network of authorized representatives provide customers with the following
types of assistance: sales, technical support, applications, and instrument repair.
If you need assistance, please contact your local Gilson representative. Specific contact information can be
found at www.gilson.com. To help us serve you quickly and efficiently, please refer to Before Calling Us on
page 51.
Inspect Shipment
Customer Service
Terms and Abbreviations
The following table defines terms and abbreviations that may appear in this document.
Term
Definition
ESI
Electrospray Ionization
SFI
Split Flow Interface
m/z ratio
Mass-to-charge ratio
MSDS
Material Safety Data Sheet
LED
Light Emitting Diode
PRV
Pressure Relief Valve
Trademarks
The following trademarks may appear in this document:
●● Microsaic®, Masscape®, and ionchip® are registered trademarks of Microsaic Systems plc in the European
Community and the United States.
●● spraychip® is a registered trademark of Microsaic Systems plc in the European Community.
●● vac-chip™ and MiDas™ are trademarks of Microsaic Systems plc in the European Community and the
United States.
All other trademarks within are trademarks or registered trademarks of Gilson, Inc.
17
Technical Specifications
MiDas™ Technical Specifications
Pump
Technical Specification
Description
Flow Accuracy
±1% at 1 mL/min
Flow Range
0.001–9.999 mL/min
Max Pressure
400 bar (5802 psi) at 9.999 mL/min
Splitter
Description
Max Pressure
69 bar (1001 psi)
Switching Frequency Range
0.2–2.0 Hz
Description
Max Pressure
414 bar (6005 psi)
Port to Port Volume
0.28–0.50 µL
Stop – Stop Actuation Speed
100–280 ms
MiDas™ Technical Specifications (continued on page 19)
18
Introduction | MiDas™
Other Specifications
Description
Connections
USB controlled via VERITY® 1900 MS Detector
Dimensions (L x W x H)
36 x 35.5 x 16.5 cm (14.2 x 14 x 6.5 in.)
Environmental Conditions
Indoor use
Altitude: Up to 2000 m
Power Requirements
Line Voltage: 100–240V AC ±10%
Line Frequency: 50/60 Hz
Power: 50 W
Overvoltage Category II: For transient overvoltages typically present on the
mains supply
For temporary overvoltage on the mains supply
Relative Humidity
Operation: 40%–80%
Pollution Degree 2: Normally only non-conductive pollution occurs. Occasionally,
however, a temporary conductivity caused by condensation must be expected
Storage: 10%–90%
Temperature
Operation: 15°C–30°C
Storage : 4°C–40°C
Weight
11.8 kg (26 lbs.)
19
2 Installation
Chapter Two
This chapter includes information about setup, including:
●● Setup on page 22
●● Front Panel on page 23
●● Plumbing Connections on page 25
●● Rear Panel Connections on page 32
21
Setup
Setup
The diagram shows the main components of
the MiDas™. Refer to the labels listed in the
table below. The MiDas™ can be stacked on the
VERITY® 1900 MS Detector.
MiDas™ Components
Connection
Description
M
MiDas™
N
Front Cover
O
Gutter
P
Drain Tube
To set up the MiDas™:
Figure 2: Exploded View of the MiDas™
1. Connect the drain tube (P) to the outlet on the
gutter.
2. Place the front feet of the MiDas™ through the holes in the gutter.
3. Attach the front cover. Magnets hold it in place and it fits loosely.
22
NOTICE
The drip tray (not pictured) is designed to catch minor leaks and should not be relied
upon as a permanent part of the fluidics handling. Make sure the fluidics path is
constructed out of the correct components and is leak free prior to extended operation.
Fix all leaks immediately upon discovery.
AVIS
Le bac de récupération montré est conçu pour attraper les petites fuites et ne doit pas
être perçu comme une partie intégrante de la manipulation fluidique. Assurez-vous
que le chemin fluidique construit à partir des composants est correct et sans fuite avant
l’utilisation prolongée. Réparez les fuites immédiatement après la découverte.
Installation | MiDas™
The figures on the following pages show the ports on the front panel of the MiDas™. Refer to the labels listed
below during operation.
MiDas™ Front Panel
Connection
Front Panel
Front Panel
Description
C
Direct Access Opening
D
Communications LED
Figure 3: Front Panel with Fluidic Ports Covered
E
Refer to Direct Sampling Valve on page 27.
F
Splitter
Refer to Splitter on page 27.
G
Make Up Pump Outlet
Refer to Make Up Pump on page 28.
H
Make Up Pump Inlet
Refer to Make Up Pump on page 28.
Figure 4: Front Panel without Cover Showing Fluidic Ports
23
Front Panel
MiDas™ Front Panel
Connection
Description
I
Pressure Bypass Assembly
Refer to Pressure Bypass Assembly on page 30.
J
Pressure Relief Valve Assembly
Refer to Pressure Relief Valve Assembly on page 31.
Figure 5: Location of Pressure Bypass and Pressure Relief Valve Assemblies
NOTICE
AVIS
24
Never disconnect the tubing while the unit is in operation. Liquid splashing might
cause physical damage.
Ne pas débrancher les tuyaux de l’outil durant l’opération. Les éclaboussures
peuvent causer d’importants dégâts.
Refer to the following diagram when making the plumbing connections described in this chapter.
C
D
D
9A
7B
7C
A
1A
2A
B
A
A
3
A
2
4
1
A
A
3
7A
6
A
4
Plumbing Connections
A
5
2B
A
T
1B
2C
E
A
Z
U
A
D
S
D
10
F
G
F
10Plumbing Connections
Figure 6:
9B
6
Connection
Tubing
Fitting
Input
Destination
1A
1/16" OD PEEK
Coned 10-32 PEEK
Splitter
Column
Coned 10-32 PEEK
Splitter
UV/VIS
Coned 10-32 PEEK
Splitter
4-Way Tee,
0.002" ID Bore
2B
Coned 10-32 PEEK
Splitter
3-Way Tee,
0.002" ID Bore
2C
Coned 10-32 PEEK
¼-28 Flangeless
3-Way Tee, 0.002" ID Bore
Backpressure
Regulator,
100 psi
1B
2A
1/16" OD
0.005" ID PEEK
Plumbing Connections Table (continued on page 26)
25
26
Connection
Tubing
Fitting
Input
Destination
3
1/16" OD
0.005" ID PEEK
Coned 10-32 PEEK
Make Up Pump
Outlet
4
1/16" OD
0.0025" ID PEEK
Coned 10-32 PEEK
3-Way Tee
6
1/8" OD PTFE
¼–28 Flangeless
1/8" Tefzel
Make Up
Pump Inlet
Reservoir
7A
3 mm OD
1 mm ID Silicone
Make Up Pump
Waste Outlet
Waste
7B
Make Up Pump
Backflushing Inlet
Waste
7C
Make Up Pump
Backflushing Inlet
Waste
Split Flow Interface
Waste
8
1/32" OD
0.010" ID PEEK
9A
1/16" OD
0.020" ID ETFE
Coned 10-32 PEEK
¼-28 Flangeless
Backpressure
Regulator,
750 psi
9B
1/16" OD
0.020¼ ID ETFE
1/4-28 Flangeless
Backpressure
Regulator, 750 psi
Waste
10
1/32" OD
0.005" ID PEEK
¼-28 Flangeless
Coned 6/32 PEEK
Backpressure
Regulator, 100 psi
Inline Microfilter
Split Flow
Interface
The direct sampling valve has six fluidic ports numbered counterclockwise from 1 as shown in the figure.
Refer to the port description in the table below.
Direct Sampling Valve Port Specification
Ports
Description
Fluidic Ports
Pressure:
Tubing:
Fitting:
0–60 bar (0–1000 psi)
1/16" PEEK
Coned 10-32 PEEK
spanner
Figure 7: Direct Sampling Valve Diagram
Operation
Attenuator
Flow
2
6
4
5
Inject
1
3
6
4
Syringe
2
1
3
Attenuator
Flow
Waste
Syringe
Position 1
5
Load
Waste
Position 2
Figure 8: Direct Sampling Valve Positions (States)
This can be set in two states as shown:
1. Inject: the loop is connected to the splitter and an external pump can deliver the sample for analysis.
2. Load: the loop is connected to the syringe port and waste ready for filling with sample.
Splitter
The splitter has four fluidics ports as shown. Refer to the port
descriptions in the table below and then make the tubing
connections.
Port Specification for the Splitter
Ports
Description
Fluidic Ports
Pressure:
Tubing:
Fitting:
Replacement
Part Numbers:
Sample side
From
column
Make Up side
From make‑up
pump
To UV/VIS
0–60 bar (0–1000 psi)
1/16" OD PEEK
Coned 10-32 PEEK
Sample Side 49040130
Make Up Side 490410120
To pressure
bypass
Figure 9: Splitter Ports Diagram
27
Make Up Pump
The make up pump has four fluidics ports as shown below. Refer to the port descriptions in the table below:
Backflushing Inlets
Make Up Outlet
Purge Valve
Pump Head
Waste Outlet
Make Up Inlet
Figure 10: Make Up Pump Ports Diagram
Make Up Pump Ports
28
Port
Description
Make Up Pump Outlet
Tubing:
Fitting:
Replacement Part Number:
1/16" ID PEEK
Coned 10-32 PEEK
490410120
Waste Outlet
Tubing:
3 mm OD 1 mm ID Silicone
Backflushing Inlets
Pressure :
Tubing:
0 bar (0 psi)
3 mm OD 1 mm ID Silicone
Make Up Pump Inlet
Pressure:
Tubing:
Fitting:
0 bar (0 psi)
1/8" OD PTFE
¼-28 Flangeless 1/8" Tefzel
NOTE
Make Up Line to Pump Head
Make sure that the tapered side of the ferrule is pointed
towards the fitting.
1. Push the tubing (D) through the fitting (C) and ferrule (B).
2. Insert the tubing end as far as possible into the inlet of the pump head (A).
3. Tighten the fitting by hand.
Add Solvent to the Make Up Pump Reservoir Bottle
WARNING
AVERTISSEMENT
Potentially hazardous chemicals can be used with
the MiDas™. Use care when handling chemicals
and wear appropriate PPE.
Figure 11: Make Up Line to
Pump Head Connection
Des produits chimiques potentiellement
dangereux peuvent être utilisés avec le MiDas™.
Faire preuve de prudence lors de la manipulation
des produits chimiques et porter des EPI
appropriés.
1. Place the make up reservoir bottle in a suitably ventilated area.
2. Add make up solvent to the make up reservoir bottle.
3. Degas the solvent by sonicating it for ten minutes.
4. Carefully reattach the tubing to the pump and make sure that the filter is immersed in the solvent.
5. It is recommended to prime the make up pump, refer to Prime the Make Up Pump on page 36.
29
Connect a pressure bypass to improve signal stability. This arrangement (shown below) serves to smooth out
pressure changes over the splitter cycling.
To Splitter Inlet
From Splitter Outlet
Backpressure
Regulator
Bypass Outlet
To Pressure Relief
Valve Assembly
Bypass
To VERITY® 1900 MS Detector
From Make Up Pump Outlet
Figure 12: Details of the Fluidic Path in the Pressure Bypass
(Part Number 14417010)
30
Component
Description
From Make Up Pump Outlet
Length:
Fitting:
1/16" 0.005" ID PEEK x 150 mm
1 x Coned 10-32 PEEK
Bypass
Tubing:
Fitting:
1/16" OD 0.0025" ID PEEK x 90 mm
Splitter Inlet/Outlet
Tubing:
Fitting:
Replacement Part Number:
1/16" OD 0.005" ID PEEK x 60 mm
490410120
Bypass Outlet
Tubing:
Fitting:
1/16" OD 0.005" ID PEEK x 60 mm
Coned 10-32 PEEK, ¼-28 Flangeless
4-way Tee
Fitting:
Bore:
Coned 10-32 PEEK
0.020" ID
3-way Tee
Fitting:
Bore:
Coned 10-32 PEEK, ¼-28 Flangeless
0.020" ID
Backpressure Regulator
Fitting:
Pressure:
¼-28 Flangeless
7 bar, 100 psi
To protect the splitter from overpressure by the make up pump, a pressure relief valve is present between
the pump and the splitter. A pressure in excess of 750 psi (52 bar) causes the relief valve to open and divert
flow to waste. The configuration of the pressure relief assembly is shown below.
Figure 13: Flow Path Through Pressure Relief Valve
(Part Number 14417011)
Component
Description
Relief Valve Inlet
Length:
1/16" OD 0.020" ID ETFE x 50 mm
Relief Valve
(Backpressure Regulator)
Fitting:
Pressure:
¼-28 Flangeless
52 bar, 750 psi
Relief Valve Outlet
Tubing:
1/16" OD 0.020" ID ETFE x 1000 mm
31
Rear Panel Connections
Refer to the rear panel diagram and instructions that follow when making rear panel connections.
USB communication port
Power socket
Figure 14: Rear Panel Diagram
USB Cable Connection
To make the USB cable connection between the MiDas™ and the
VERITY® 1900 MS Detector, use the supplied USB cable. Use the
end with the “A-type” (flat) connector to connect to the
VERITY® 1900 MS Detector and use the end with the “B-type”
(square) connector to connect to the MiDas™.
Figure 15: USB Cable
with “A-Type” Connector
Power Cord Connection
Locate the appropriate power cord for your line voltage, connect
the power cord to the power socket on the MiDas™, and then to a
grounded power outlet.
The power supply in the MiDas™ accepts any line voltage in the
range 100–240 V. There is no voltage selector at the rear of the
MiDas™ and there are no externally accessible fuses.
Figure 16: USB Cable
with “B-Type” Connector
After powering up both the VERITY® 1900 MS Detector and the MiDas™, the communications LED should
display as solid green. A flashing LED indicates no communication (refer to the Troubleshooting chapter).
32
WARNING
CAUTION
WARNING
ATTENTION
When installing or operating the instrument you must ensure that there is
sufficient space behind the instrument to unplug the power cord.
Lors de l’utilisation MiDas™ il doit être possible de déconnecter l’appareil
de l’alimentation secteur à tout moment. En cas d’urgence, le connecteur
d’alimentation de l’instrument doit être facilement accessible et
débranchable.
When operating MiDas™ it must be possible to disconnect the instrument
from mains supply at any time. In the event of an emergency the power
connector of the instrument must be easily accessible and removable.
Lors de l’installation ou l’utilisation de l’instrument vous devez vous assurer
qu’il ya suffisamment d’espace derrière l’appareil pour débrancher le cordon
d’alimentation.
Never use any cabling not supplied or recommended by Gilson. Use of
unspecified cabling may lead to improper operation or failure to comply with
safety or EMC regulations.
MiDas™ must never be operated from a power outlet that has no ground
connection. The absence of a ground connection can lead to electric shock or
short circuit.
WARNING
Never use a power cord other than that supplied by Gilson. The use of an
inadequately rated power cord can lead to electric shock or short circuit.
WARNING
The MiDas™ is used to analyze liquid samples, liquid contact with external
equipment may lead to the risk of electric shock or short circuit. The user must
ensure that fluidic connections are not close to ancillary equipment and are
checked for leaks prior to use. In the event of a leak, any ancillary equipment
not designed for use with liquids must be turned off until the liquid is
removed.
Ne jamais utiliser des câbles non fournis ou recommandés par Gilson.
L’utilisation de câbles non spécifié peut entraîner un mauvais fonctionnement
ou du non-respect de la sécurité ou de la compatibilité électromagnétique.
MiDas™ ne doit jamais être utilisé à partir d’une prise de courant qui n’a pas
de connexion à la terre. L’absence de mise à la terre peut provoquer un choc
électrique ou un court-circuit.
WARNING
AVERTISSEMENT
N’utilisez jamais un cordon d’alimentation autre que celui fourni par Gilson.
L’utilisation d’un cordon d’alimentation non-adéquat peut provoquer un choc
électrique ou un court-circuit.
MiDas™ est utilisé pour analyser des échantillons liquides, le contact du
liquide avec un équipement externe peut entraîner un risque de choc
électrique ou de court-circuit. L’utilisateur doit s’assurer que les connexions
fluidiques ne sont pas près de l’équipement.
33
3 Operation
Chapter Three
This chapter contains information about:
●● Prime the Make Up Pump on page 36
●● Set Make Up Pump Flow Rate on page 37
●● Set Splitter Split Ratio on page 37
NOTICE
AVIS
Condensation caused by relocating MiDas™ to new ambient conditions could cause
hardware failure within MiDas™. Allow device to acclimatise for three hours before
powering on a recently moved system.
La condensation provoquée par le déplacement du MiDas™ dans de nouvelles
conditions ambiantes pourrait provoquer une défaillance. Laissez MiDas™ s’acclimater
pendant three heures avant de mettre l’appareil sous tension.
35
Prime the Make Up Pump
Prime the Make Up Pump
NOTICE
Running the pump when dry can cause damage to the pump. Ensure that the pump
has been primed and pistons have been backflushed (refer to Backflush the Pistons
on page 43) before starting pumping.
Check regularly that the make up pump solvent reservoir contains enough fluid and
does not run dry during operation.
Utiliser la pompe à l’état sec peut causer des dommages à la pompe. Assurezvous que la pompe a été amorcée et les pistons ont été contre-rincés
(Backflush the Pistons on page 43) avant de commencer le pompage.
AVIS
Vérifiez régulièrement que le réservoir de maquillage contient suffisamment de
liquide et ne tarit pas en cours de fonctionnement.
Back Flushing Inlets
Refer to the diagram when following this procedure.
1. Make sure that the filter is
immersed in the make up
reservoir.
Make Up Outlet
2. Twist the black purge valve
one turn counterclockwise to
open it.
3. Attach the supplied plastic
syringe using the metal
adaptor and silicone tubing
to the waste outlet.
Purge Valve
Pump Head
4. Draw 5–10 mL of solvent from
the reservoir by repeatedly
filling the syringe, detaching
Waste Outlet
it, and then emptying to an
appropriate waste container.
5. Connect one end of the
silicone tubing to the metal
pipe of the purge valve and
the other to a solvent waste
container.
Make Up Inlet
Figure 17: Make Up Pump Ports Diagram
6. Prime the pump for a few minutes at high flow (5–10 mL/min).
7. When satisfied that the line to the pump is filled and liquid is being drawn through the pump in a
continuous stream, stop the pump and then shut the purge valve by turning it fingertight clockwise.
The pump is now primed and ready to dispense make up pump solvent.
NOTE
36
This operation should be performed on any change to the make up composition to avoid the
possible appearance of solvent fronts mid-experiment.
Inappropriately fast flow rates can lead to sample outgassing and bubble formation. These
bubbles may alter dispensed volume and hence sample accuracy.
Operation | MiDas™
Set the make up pump flow rate at an appropriate level so that after splitting in the
split flow interface (SFI), the spraychip receives no more than 2 µL/min. The standard split ratio supplied
with the VERITY® 1900 MS Detector is 1000:1. With the supplied spilt the maximum should therefore be
2000 µL/min (2 mL/min). If greater dilution is required then the VERITY® 1900 MS Detector split can be
adjusted to a higher ratio (refer to the VERITY® 1900 MS Detector User’s Guide for details on adjusting the split).
NOTE
Use of higher source flow rates than 2 µL/min can lead to unreliable
electrospray ionization (ESI) conditions and shortened spraychip® and vac-chip™ lifetimes.
Set Splitter Split Ratio
Set Make Up Pump Flow Rate
Set Make Up Pump Flow Rate
The split ratio of the splitter should be set to a level appropriate for the application, and can be set by
program number or by allowing the software to calculate the split factor. Care should be taken to ensure
resultant flow rates are compatible with the VERITY® 1900 MS Detector (refer to the VERITY® 1900 MS Detector
User’s Guide).
H PLC Flow Rate (mL/min)
Split Ratio
Figure 18: Recommended Split Factor Based on Flow Rate and How to Calculate a Split Ratio
Source: Data from MRA™ Operating Manual, Rheodyne, L.P., 2320949A(1), 8/01.
37
4 Maintenance
Chapter Four
The following maintenance may be carried out by users:
●● Direct Sampling Valve Replacement on page 41
●● Splitter Service on page 42
●● Replace the Pump Head on page 44
●● Clean the Check Valves on page 46
You are only permitted to perform the maintenance tasks described in this user’s guide. If additional
maintenance is required, contact your local Gilson representative (refer to Customer Service on page 17).
39
Maintenance Preparation
Maintenance Preparation
Before maintenance can begin, the MiDas™ must be decontaminated by removing any hazardous chemicals
used by the instrument. Further, the instrument should be disconnected from any other instrument and the
unit MUST be disconnected from the power supply. To protect the electronic components of the MiDas™
from ESD, the rear chassis of the MiDas™ should be connected to an electronic ground either through the
ground pin in the mains power supply or a grounding clip. After disconnecting the unit from the mains, wait
at least one minute before attempting any maintenance.
WARNING
CAUTION
WARNING
ATTENTION
When operating MiDas™ it must be possible to disconnect the instrument from
mains supply at any time. In the event of an emergency, the power connector of the
instrument must be easily accessible and removable.
Lors de l’utilisation MiDas™ il doit être possible de déconnecter l’appareil de
l’alimentation secteur à tout moment. En cas d’urgence, le connecteur d’alimentation
de l’instrument doit être facilement accessible et débranchable.
Cleaning and Decontamination
Even if contamination is not suspected or seen, the exterior surfaces of the MiDas™ should be wiped
down with 10% aqueous IPA to remove any residues. If any parts of the assemblies need to be removed or
disassembled then these parts should also be checked for signs of contamination. The fluid lines should be
flushed with 10% aqueous IPA to remove any residues inside the system.
Potentially hazardous chemicals can be used with the MiDas™. Use care when
handling chemicals and wear appropriate PPE.
WARNING
AVERTISSEMENT
40
Maintenance | MiDas™
Des produits chimiques potentiellement dangereux peuvent être utilisés avec le
MiDas™. Faire preuve de prudence lors de la manipulation des produits chimiques
et porter des EPI appropriés.
Position Valve for Replacement
Ensure that the valve driver is in the correct orientation for replacement of the valve (position 1).
Remove Valve Driver
After positioning the valve as described above, remove all fluidics connectors and loosen the spanner that
holds the valve in place. Remove spanner completely. Do not use a wrench for this step. Spanner should be
tightened and loosened using only your hand.
Direct Sampling Valve Replacement
Direct Sampling Valve Replacement
Figure 19: Correct Orientation of the Direct Sampling Valve with Ports 1, 2, and the Clocking Groove at the Top
Install Replacement Valve
The shaft of the replacement valve must be oriented in Position 1 prior to insertion into the valve driver
body. In Position 1, the flat of the shaft is oriented perpendicular to the marking on the reverse of the
valve body. Insert the valve into the valve driver in the orientation shown in the figure above and note the
orientation of the numbered ports. Ports 1, 2, and narrow clocking groove will always be at the top when the
valve driver body is oriented correctly.
41
Splitter Service
Splitter Service
Normal use of the splitter will provide one million samplings. The main cause of early failure, which is seen
as valve leakage, is abrasive particles in the sample and/or mobile phase or precipitation of concentrated
solutions. Either can cause scratches on the rotor seal and stator face assembly. In order to service the
splitter, you will need to obtain a splitter maintenance kit (part number 18007044).
Disassemble Valve
To disassemble the splitter valve, refer to the
figure above, and then proceed as follows:
1. With the hex key provided in the splitter
maintenance kit, remove the stator screws
from the stator.
2. Remove the stator and stator face assembly
from the stator ring. The stator face
assembly usually remains on the stator.
3. Remove the stator ring.
4. Remove the rotor seal from the valve body.
The rotor seal is mounted on three pins and
can be pulled off.
Reassemble Valve
NOTE
Before reassembly, use clean,
filtered compressed gas to blow
out the shaft assembly area of
the valve.
1. Mount the new rotor seal with the slots
facing the stator. The three pins on the
shaft assembly fit into the mating holes in
the rotor seal only one way.
Figure 20: Exploded View of the Splitter
2. Replace the stator ring so the two short pins enter the mating holes in the body.
3. Mount the new stator face assembly onto the stator. The pins on the assembly fit into the mating holes
on the stator only one way.
4. Replace the stator and stator face assembly on the valve so that the pin in the stator ring enters the
mating hole in the stator.
5. Replace the stator screws into the stator. Tighten each an equal amount until the screws are fingertight,
then turn another half turn.
42
Make Up Pump Service
Backflush the Pistons
When you flush the pistons regularly, the service life of the
seals and pistons increases. While flushing, contaminants are
washed from the rear piston area. Refer to the figure below
when attempting this operation.
Flushing Solution
The following solvents are recommended for flushing the
pistons:
Figure 21: Flushing the Pistons in the Pump Head
●● Water
●● Mixture of 80% water and 20% ethanol
●● Isopropanol
1. Open the black purge valve adjacent to the pump head.
2. Connect the outlet to the waste container with a length of supplied silicone tubing (1).
3. Connect the inlet to the syringe filled with flushing solution with the supplied silicone tubing (2).
4. Fill up flushing solution with the syringe through the pump head until there are no more air bubbles
running through the waste bottle.
5. Afterwards, remove the tubing 1 and tubing 2 and connect inlet and outlet with a tubing.
Switch Off the Pump
If you want to switch off the pump for a longer term, flush the pump head with isopropanol.
43
Replace the Pump Head
Aggressive or toxic solvent residue can irritate the skin! Wear appropriate PPE.
WARNING
Les résidus de solvant agressif ou toxique peuvent irriter la peau! Porter des
EPI appropriés
AVERTISSEMENT
NOTICE
AVIS
Mishandled piston rods can break. Before disassembling the pump head,
remove the two piston rods and deposit in the correct orientation. When
assembling the pump head, the piston rods must be inserted on the same
side they have been removed from.
Les tiges de piston maltraitées peuvent se briser. Avant de démonter la tête
de pompe, enlever les deux tiges de piston et posez-les dans le bon sens. Lors
du montage de la tête de pompe, les tiges de piston doivent être insérés sur
le même côté dont ils ont été retirés.
Remove the Pump Head
NOTE
Ensure that the pump head has been
flushed with suitable solvent.
1. Unscrew inlet fitting (2) and outlet fitting (1).
2. Unscrew the inlet fitting (5).
3. Unscrew the outlet fitting (4) to the pressure sensor.
4. Alternately unscrew the four fastening screws (3).
5. Hold the pump head and consecutively pull out all
fastening screws.
Figure 22: Pump Head
44
1. Insert the fastening screws (3) and tighten alternately.
2. Tighten the outlet fitting (4) to the pressure sensor.
3. Tighten the inlet fitting (5).
4. Tighten the inlet fitting (2) and the outlet fitting (1) of the piston.
NOTICE
AVIS
Overtightening capillary fittings can damage the pump head. Check the torque of
screw fittings. Appropriate torques is 5 Nm for stainless steel fittings and 0.5 Nm for
PEEK fittings.
Install the Pump Head
Le serrage des raccords capillaires peut endommager la tête de la pompe. Vérifiez le
couple des raccords vissés. Les couples appropriés sont de 5 Nm pour des raccords en
acier inoxydable et 0,5 Nm pour les raccords en PEEK.
45
Clean the Check Valves
Dirty check valves do not open and close properly. They can cause pressure fluctuations and irregular flow.
Figure 23: Pump Head
NOTE
Ensure that the pump head has been flushed with suitable solvent.
Remove the Check Valves
NOTE
Loosen the screw fittings of the capillary connections alternately, to prevent the capillaries
from bending.
Pump head should be flushed prior to procedure.
1. Unscrew the inlet fitting (1) and the outlet fitting (2). Remove the check valve.
2. Unscrew the inlet fitting (5) and remove the check valve.
Clean the Check Valves
1. Fill a beaker with solvent.
2. Place the valve in the beaker.
3. Put the beaker in an ultrasonic bath for at least ten minutes.
46
NOTE
Install the Check Valves
Insert the valves in the direction of flow as shown in the figure below.
Figure 24: Check Valve Showing
Ball (1) and Flow Direction (2)
1. Insert the check valve and tighten the inlet fitting (5).
2. Insert the check valve. Tighten the inlet fitting (1) and the outlet fitting (2).
3. Tighten the outlet fitting (2) and the inlet fitting (5) with 7.5 Nm.
47
Safe State Verification
Safe State Verification
After maintenance, the MiDas™ should be tested to verify that it is in a safe state.
Monitor the make up pump pressure at 1000 µL/min (1 mL/min) when infusing methanol, 0.1% formic acid
through the split flow interface (SFI). If the pressure is not within 10–25 bar, then immediately switch off the
MiDas™ and disconnect the instrument from the mains power supply and any attached equipment. Do not
use the instrument. Contact Gilson.
WARNING
CAUTION
WARNING
ATTENTION
When operating MiDas™ it must be possible to disconnect the instrument
from mains supply at any time. In the event of an emergency the power
connector of the instrument must be easily accessible and removable.
Lors de l’utilisation MiDas™ il doit être possible de déconnecter l’appareil
de l’alimentation secteur à tout moment. En cas d’urgence, le connecteur
d’alimentation de l’instrument doit être facilement accessible et
débranchable.
Potentially hazardous chemicals can be used with the MiDas™. Use care when
handling chemicals and wear appropriate PPE.
WARNING
AVERTISSEMENT
48
Des produits chimiques potentiellement dangereux peuvent être utilisés avec
le MiDas™. Faire preuve de prudence lors de la manipulation des produits
chimiques et porter des EPI appropriés.
5 Troubleshooting
Chapter Five
Make sure the VERITY® 1900 MS Detector is fully operational first. Refer to the VERITY® 1900 MS Detector
User’s Guide troubleshooting chapter for details.
Troubleshooting Table
Symptom
Cause
Remedy
MiDas™ does not respond to
commands.
No communications
(communication LED on
MiDas™’ front panel flashes
constantly).
Check the USB connection
between the MiDas™ and the
VERITY® 1900 MS Detector. Check that
the USB smartlock software dongle
has been connected and then reboot
the software.
MiDas™ is not powered up
(communication LED on
MiDas™ front panel is off ).
Check power connection.
I have no signal during
sampling acquisition.
The
VERITY® 1900 MS Detector
is not ready for a sampling
experiment.
Refer to the VERITY® 1900 MS Detector
User’s Guide for troubleshooting
advice.
I have signal, but cannot see
my direct sample.
Excessive chemical noise is
swamping signal.
Refer to the VERITY® 1900 MS Detector
User’s Guide for troubleshooting
advice.
The splitter is not
operating (MiDas™ is
generating a constant
‘pulsing’ sound)
Contact Gilson.
The loop injection is not
operating (MiDas™ should
emit an audible ‘click’ when
switching the valve).
Contact Gilson.
49

Symptom
Cause
Remedy
Make up pump solvent
is not dispensed or
flow rate/pressure is erratic.
Pump not primed.
See Prime the Make Up Pump on
page 36.
Inlet solvent flow blocked.
Check reservoir level.
Check reservoir filter and replace, if
necessary.
Check valves blocked.
See Clean the Check Valves on
page 46.
Check valves defective.
See Clean the Check Valves on
page 46.
Pump head seals defective.
If liquid emerges behind the pump
head in the back flush, contact Gilson.
The pressure rating of the
valve has been exceeded.
Check that the flow path is not
blocked causing abnormal back
pressure, and then reduce flow rates
to the splitter to reduce pressure.
The rotor seal and stator
face assembly have been
damaged by abrasive
particles in the sample
and/or mobile phase or
crystallization of buffer
solutions.
Replace the stator/rotor and contact
Gilson for a maintenance kit.
Note: Flush the valve frequently to
prevent crystallization.
Splitter leaks between the
stator and stator ring.
50
Troubleshooting | MiDas™
Before Calling Us
Your local Gilson representative will be able to serve you more efficiently if you have the following
information:
●● Serial number and model number of the instruments involved
○○ The serial number is located on the rear panel
●● Installation procedure you used
●● List of concise symptoms
Repair and Return Policies
●● List of operating procedures and conditions you were using when the problem arose
●● List of other devices connected to the instrument and a description of those connections
●● List of other electrical connections in the room
●● Diagnostics report
Warranty Repair
Units covered under warranty will be repaired and returned to you at no charge. If you have any questions
about applicability, please contact your local Gilson representative.
Warranty for Non-Gilson Equipment
Contact your local Gilson representative. Gilson, Inc. will coordinate with the manufacturer for handling
warranty-related service. The warranties are those provided by the manufacturer.
Non-Warranty Repair
For out-of-warranty repairs, contact your local Gilson representative who will discuss service options with
you and can assist in making arrangements to return the equipment, if necessary.
51
Return Procedure
Contact your local Gilson representative to obtain authorization before returning any Gilson equipment. To
return a piece of equipment:
●● Carefully pack the unit to prevent damage in transit. Check with your local Gilson representative
regarding proper method of shipment. No responsibility is assumed by Gilson or your local Gilson
representative for damage caused by improperly packaged instruments. Indicate the authorization on
the carton and on the packing slip.
●● Always insure for the replacement value of the unit.
●● Include a description of symptoms, your name, address, phone number, and purchase order to cover
repair costs, return and shipping charges, if your institution requires it.
Unit End-of-Life
When a unit reaches the end of its useful life, refer to www.gilson.com for directions and
information on the end-of-life policy. This is in accordance with the European Union Directive
2002/96/EC on Waste Electrical and Electronic Equipment (WEEE).
52
Troubleshooting | MiDas™
A Replacement Parts
Appendix A
Part Number
Description
14417010
VERITY 1900, MIDAS PRESSURE BYPASS ASSY
14417011
VERITY 1900, MIDAS PRES RELIEF ASSY
14417012
VERITY 1900, MIDAS INJ VLV REBUILD KIT
14417013
VERITY 1900, MIDAS PUMP CHECK VLV
18007044
MRA SPLITTER MAINTENANCE KIT
490410120
F120, FINGERTIGHT FITTING
Used for Make Up Pump Outlet and Splitter Make Up Side
49040130
NUT,LONG FINGER TIGHT,PEEK NAT, (F-130)
Used for Splitter Sample Side
53
B Chemical Compatibility Chart
Appendix B
This appendix allows you to either check which materials will work with the chemicals you are using or
planning to use; or to view what compatibility rating given to a material you are planning to use. Provided
courtesy of IDEX Health & Science LLC
Chemical rating key:
A = Minor Issue.
NOTE
The substance has little interaction with the material. Prolonged exposure may cause material
deterioration.
B = Use Caution.
NOTE
The substance has slight interaction with the material. Be cautious when using with tubing
and frits. Exposure may cause material deterioration
C = Incompatible.
NOTE
The substance is not recommended to be used with the material.
Chemical Compatibility Chart
Substance
Fraction
Acetic anhydride
Aluminum acetate
100%
Aluminum chloride
Severity
Material
Notes
B
Stainless Steel
B
Stainless Steel
C
Stainless Steel
Aluminum hydroxide
10%
B
Stainless Steel
Aluminum sulfate
100%
B
Stainless Steel
Ammonium bicarbonate
90%
B
Stainless Steel
Chemical Compatibility Chart (continued on page 56)
55
Substance
Fraction
Ammonium carbonate
Severity
Material
Notes
B
Stainless Steel
Elevated temperatures may
reduce resistance.
reduce resistance.
Ammonium chloride
10%
B
Stainless Steel
Ammonium oxalate
10%
B
Stainless Steel
Ammonium sulfite
10%
B
Stainless Steel
Aniline
10%
B
Stainless Steel
Anisole
B
UHMWPE
Aqua regia
C
PEEK, UHMWPE
Barium carbonate
B
Stainless Steel
B
Stainless Steel
Barium nitrate
B
Stainless Steel
Barium sulfate
B
Stainless Steel
Benzene
B
Stainless Steel,
UHMWPE
Benzene sulfonic acid
C
PEEK
B
Stainless Steel
B
UHMWPE
B
Stainless Steel
C
PEEK
C
PEEK, UHMWPE
Bromine water
C
Stainless Steel
Bromochloromethane
C
UHMWPE
Barium chloride
Benzoic acid
10%
60%
Benzoyl chloride
Boric acid
100%
Bromine (dry)
Bromine (wet)
saturated
reduce resistance.
Butyric acid
100%
B
Stainless Steel
Calcium carbonate
100%
B
Stainless Steel
Calcium chlorate
10%
B
Stainless Steel
Calcium chloride
10%
C
Stainless Steel
56
Chemical Compatibility Chart | MiDas™
Substance
Fraction
Calcium hydroxide
50%
Severity
Material
Notes
B
Stainless Steel
B
Stainless Steel
B
Stainless Steel
Carbon disulfide
B
Stainless Steel,
UHMWPE
Carbon tetrachloride
B
Stainless Steel,
UHMWPE
Carbonic acid
B
Stainless Steel
Chloric acid
C
Stainless Steel
B
Stainless Steel
Chlorine
C
PEEK ,UHMWPE
Chlorine gas
B
Stainless Steel,
UHMWPE
Chlorine liquid
B
PCTFE, UHMWPE
reduce resistance.
Chlorobenzene
B
PCTFE
Elevated temperatures reduce
resistance.
Chloroform
B
PCTFE
A
PEEK
Not recommended for use
with PEEK rotor seals.
B
UHMWPE
C
PEEK, UHMWPE
B
PCTFE
B
Stainless Steel
C
PEEK
Calcium sulfate
Carbolic acid
Chlorinated water
Chlorosulfonic acid
100%
saturated
10%
Chlorotrifluoroethylene
Chromic acid
conc.
Citric acid
10%
B
Stainless Steel
Copper carbonate
10%
B
Stainless Steel
Copper chloride
5%
aerated
C
Stainless Steel
Subject to pitting.
Elevated temperatures and
prolonged exposure may
reduce resistance.
Subject to pitting.
Subject to pitting.
57
Substance
Fraction
Severity
Material
Notes
Copper cyanide
100%
B
Stainless Steel
Copper sulfate
100%
B
Stainless Steel
Creosote oil
B
Stainless Steel
Cyanogen gas
B
Stainless Steel
Dichlorobenzene (o-)
B
UHMWPE
reduce resistance.
Dichlorobenzene (p-)
B
UHMWPE
reduce resistance.
Dichloroethylene
C
UHMWPE
Dimethylsulfoxide
(DMSO)
B
PEEK
Be cautious when using with
tubing and frits.
Diphenylsulfone (DPS)
B
PEEK
Be cautious when using with
tubing and frits; elevated
temperatures reduce
resistance.
Dowtherm A
C
PEEK
Dowtherm G
B
PEEK
Dowtherm HT
B
PEEK
Dowtherm LF
B
PEEK
Ethyl acetate
B
Stainless Steel
Ethyl ether
B
PCTFE
Ethyl glycol
B
Stainless Steel
Ethylene bromide
B
PCTFE
Ethylene chloride
(dichloroethane)
B
UHMWPE
Ethylene oxide
C
PCTFE
Ferric chloride
B
PEEK
C
Stainless Steel
resistance.
resistance.
58
Substance
Fraction
Severity
Material
Notes
Ferric nitrate
10%
B
Stainless Steel
Ferric sulfate
100%
B
Stainless Steel
Ferrous chloride
C
Stainless Steel
Ferrous sulfate
B
Stainless Steel
Fluorine
C
PEEK, UHMWPE
Formic acid
B
Stainless Steel,
PEEK
Freon 11
B
PCTFE
Furfural
B
Stainless Steel
Room temperature only.
Gallic acid
100%
B
Stainless Steel
Glycerin
100%
B
Stainless Steel
B
UHMWPE
Halothane
Hydrobromic acid
10%
conc.
C
reduce resistance.
PEEK
Hydrochloric acid
C
Stainless Steel
Hydrocyanic acid
B
Stainless Steel
Hydrofluoric acid (40%)
C
PEEK
Hydrofluosilic acid
C
Stainless Steel
Hydrogen peroxide
100%
B
Stainless Steel
Hydrogen sulfide
100%
B
Stainless Steel
B
PEEK
C
Stainless Steel
Iodine
Lactic acid
100%
B
Stainless Steel
Magnesium chloride
50%
B
Stainless Steel
Magnesium sulfate
100%
B
Stainless Steel
B
Stainless Steel
Malic acid
59
Substance
Fraction
Mercuric chloride
10%
Severity
Material
Notes
B
PCTFE
C
Stainless Steel
Mercury
100%
B
Stainless Steel
Methyl alcohol
100%
B
Stainless Steel
Methylcyclohexane
B
UHMWPE
reduce resistance.
Methylene chloride
A
PEEK
B
UHMWPE
Be cautious when using
with tubing and frits; Room
temperature only. Not
recommended for use with
PEEK rotor seals.
C
Stainless Steel
Muriatic acid
Naptha
100%
B
Stainless Steel
Nickel chloride
100%
B
Stainless Steel
Nitric acid
30%
conc.
B
PEEK, Stainless
Steel, UHMWPE
Prolonged exposure may
cause deterioration.
Nitrogen tetroxide
B
PCTFE
Below Room Temperature
Oils (linseed and olive)
B
UHMWPE
Oleic acid
B
Stainless Steel
Oleum
C
PEEK, UHMWPE
Oxalic acid
10%
B
Stainless Steel
reduce resistance.
Ozone
liquid
B
UHMWPE
reduce resistance.
Phenol
100%
B
Stainless Steel
C
PEEK
with tubing and frits; room
temperature only.
B
Stainless Steel
Picric acid
B
Stainless Steel
Potassium bichromate
B
Stainless Steel
Phosphoric acid
10%
60
Substance
Fraction
Severity
Material
Notes
Subject to pitting.
Potassium bromide
B
Stainless Steel
Potassium carbonate
B
Stainless Steel
Potassium chloride
10%
B
Stainless Steel
Potassium ferricyanide
10%
B
Stainless Steel
Potassium ferrocyanide
10%
B
Stainless Steel
Potassium hydroxide
10%
B
Stainless Steel
(C @ 100%)
Potassium nitrate
100%
B
Stainless Steel
reduce resistance.
B
Stainless Steel
B
Stainless Steel
Potassium sulfide
B
Stainless Steel
Pseudocumene
B
UHMWPE
Pyrogallic acid
B
Stainless Steel
Quinine Bisulfate
B
Stainless Steel
Quinine sulfate
B
Stainless Steel
Rosin
B
Stainless Steel
Silver chloride
C
Stainless Steel
Silver nitrate
B
Stainless Steel
Sodium (hot)
C
PEEK
Sodium acetate
B
Stainless Steel
Potassium oxalate
Potassium permanganate
10%
Sodium carbonate
10%
B
Stainless Steel
Sodium chloride
10%
B
Stainless Steel
Sodium chlorite Bleach
50%
B
UHMWPE
Sodium hyposulfite
100%
B
Stainless Steel
Sodium nitrate
50%
B
Stainless Steel
reduce resistance.
61
Substance
Fraction
Sodium peroxide
Severity
Material
Notes
B
UHMWPE
Sodium sulfate
20%
B
Stainless Steel
Sodium sulfide
50%
B
Stainless Steel
Stannic chloride
C
Stainless Steel
Stearic acid
B
Stainless Steel
Sulfur chloride
B
Stainless Steel
Sulfur dioxide
100%
B
Stainless Steel
Sulfur trioxide
10%
C
UHMWPE
Sulfuric acid
> 40%
conc.
B
PEEK
Prolonged exposure may
cause deterioration. (C @
100%)
Sulfuric acid (aerated)
B
Stainless Steel
reduce resistance.
Sulfuric acid (air free)
B
Stainless Steel
reduce resistance.
B
Stainless Steel
C
UHMWPE
Sulfurous acid
100%
Sulfuryl chloride
Tanic acid
100%
B
Stainless Steel
Tartaric acid
50%
B
Stainless Steel
Tetrabromoethane
B
UHMWPE
Elevated temperatures and
prolonged exposure may
reduce resistance.
Tetrahydrofuran (THF)
A
PEEK
with tubing and frits; room
temperature only. Not
recommended for use with
PEEK rotor seals.
Tetrahydrofuran (THF)
B
PCTFE
Thionyl chloride
C
UHMWPE
Thionyl chloride
B
PCTFE
Thiophene
B
UHMWPE
62
Substance
Fraction
Severity
Material
Notes
reduce resistance.
Toluene
B
UHMWPE
Trichlorethylene
B
Stainless Steel
Trifluoromethyl sulfonic
acid
C
Xylene (p-)
B
PEEK
UHMWPE
reduce resistance.
63
C Appropriate Delivery Rates
Appendix C
At low attenuation, hence high attenuator frequency, sample has to be supplied at a higher rate to fill the
rotor groove on each cycle. If this is not ensured, then a higher attenuation than expected is observed. Refer
to the table below when selecting the delivery rate.
Recommended Minimum Delivery Rate for Various Attenuation Levels
Attenuation at
0.5 mL/min
Make-up Pump Rate
Attenuation at
1 mL/min
Make-up Pump Rate
Attenuation at
2 mL/min
Make-up Pump Rate
Minimum required
delivery rate (µL/min)
1669
3337
6675
50
1250
2500
5001
50
984
1968
3935
50
833
1665
3330
50
681
1363
2725
50
644
1288
2577
50
493
985
1970
50
341
682
1364
50
265
530
1060
50
189
379
758
50
167
333
667
100
133
267
533
100
125
250
500
100
100
200
400
100
83
167
333
100
Recommended Minimum Delivery Rate for Various Attenuation Levels Table (continued on page 66)
65
Appropriate Delivery Rates
Recommended Minimum Delivery Rate for Various Attenuation Levels
Attenuation at
0.5 mL/min
Make-up Pump Rate
Attenuation at
1 mL/min
Make-up Pump Rate
Attenuation at
2 mL/min
Make-up Pump Rate
Minimum required
delivery rate (µL/min)
67
133
267
100
50
100
200
100
42
83
167
100
33
67
133
200
25
50
100
200
19
39
78
200
17
33
67
200
13
25
50
200
In Direct Sampling, a fixed volume of sample is being delivered to attenuator. This will be analyzed by the
VERITY® 1900 MS Detector over a number of scans obtained from the following relation:
Scans =
60 ∙ Vloop
Ft
Where F is the delivery rate (in µL/min) and t is the scan time (in seconds). It is recommended that analysis is
performed over at least 10 scans for reproducible results.
66
Appropriate Delivery Rates | MiDas™

MiDas - Gilson

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