5210 B. 5-Day BOD Tnt

Source . bup '"'ww!!llfMCO wPl'btmVI.bfteS! OIedpds. ~;IObfp hml
5210 BIOCHEMICAL OXYGEN DEMAND IBODr
5210 B. 5-Day BOD Tnt
1. a.n.tJIl DI.eulllon
•.
Pt~"""'.'
The mettIod 00tIIitlt or /IIng wIlh NITlple. 10 lM!l1Iowing, ., Ilrlighl bolJle of the speafIecl
IÎz. and Il1OJbltinog • • the
Il*iJ'Jed lempll1Ilul1I /0( 5 d. Disoso/Yed OXV'iIen Is rnusuAlll lnillIIlly Ind Iller
Il1OJbllIon.1I'Ill1tM 800" oomponedl'rom lhe clifI"eterlllIl befWr!en lnItiII and I\PIII 00. 8ecIUH the Intill
00 Il del.rrnIntO IhofIly .n.... 1IIe dilllllon Il mIde.lIIlllll)'gell UpIKlI oc.aJrmg illier Ihismel5Uremelllls
lnd~ ln !hl
BOO mnSlnmet'il.
II. San,..", lIId PlJ{1II: SImpIIIs lor BOO .n.1ysÎ$ fMy
_Ille
aignifieantly ôurinO ltotlQI bel' un
CCII'Idkln .ncI_ly:sI$. Ie5lJllng ln Iow BOO vllues. Minma. redI.Iction 01 BOO ~ lnI/yljng IImple
prompWy or by ooollnglt ID lIIIar·fnlezîng Idi.,e.1Itunl during stol1Ige. fkrwe __• _ " kM lempll'ltln.
UIp holding lime ID 1 iRllirTun. Wmn Cl'illed Nl'llpIe$ II) 20 1 3"c belote _lysiI.
1) GniD lII'I1lHS - Il' lnalysis il begun witIlin 2 "
01 çollec;tion, cclcIllGl1lQI .. Ullneeessllty. If
il nDt Il.rted ....... 2 Il oIlI~e eoIIIelion. kllep $IIllpIII. or b&Iow 4'C
8IgIn Il.)'IiI wilItin e Il 01 eoIecliort: 'flflItfI1tIis "
nDt
.,.Iytb
'rom lhe tirnI of CDIII<llon.
poss/bfI8 bIcIouM lM ...mping th .. dlsl.,. mm
lhlllborlllory. tIOI'I" or beIDw 4"C and rwp:)rt Itngl" and IltPlperlt.... d 1t0f'lllll""" ltM ~ ln no
cne SlIl1.f\IlyIlJ l'I'lCl1Ilh1n 24 Il Ill.... gtIlb $IlTlpIII eollllc:liQn. Wllen IlImpllls.re 10 bD used lor
In.1ysIs wIlhln e Il d eoIe-etlon.
2) CciiiipOSÎtll_ilflleS _Keep~ 81 orbetllw.OC durIrç ~ UTIIl c:ornposltlng perlod 10
regulalDly
IUT
Ils
mMe BYelY efb1 kJ deIver Simples for
24 1\. lille the SIITlll altllfla es for 5lcnge Dl' \If1Ib semples. startlng the measuntment oIllokllng
and of OOi.$!illIililll periQd. SIàe 5ltnge tme and 00 dtioIiS IS
2.
~
tme l'rom
of the reeul!lI.
Appamui
1. ~ tr:oitJIIs: Use gI_ bOItlel hlving eo ml or OlMlet CIIl*iIy (3OO-fnl bolIlII ~ 1
~Uli
Sloppllf and 1
n.ecl rnouùl . . plef-.d). Clun boldII wiU'I 1 ~Ifllent. "'l'lM l!lofouGhly.
and dreln beftlre use. A$ 1 pteCI1.IIion 1lIIin$l1ll'''i:1lI Iif lfIo Ille diIuIkIn boIllI dI.rlng lncublilofl. USI •
Wller se". otllalll SI1Isfac:lOlY waler iUIs by ~k'Q bOIt... In 1 WItIl" tlIltI or bot lCldIllg will. la t!II
Il.AllI
lTI(lljh
01 speciII 900 bollles. PlIee • paper or pIIstIc: eup or kIII cap _
n....ed l'llOlAh cf bl:taIe ID
r«1uee &laponllon of the wlt« HII duI1nQ lM"""!on.
~. ..., ~ 01: wdw biIttI, Ihermostltleal, eontrolIed " 20 .. 1"C. El<dUde Il lIgIIIlo prewm
poMibi~y d
p/I<ll<lSynthel
PfOàueIlOn 01 00.
3. RIIgtlnl$
GCH-6'2995 8101eC1uoo&osie elI\WnolCfllCflUlc H~ : 5.\.1 52lo.S
~lœl
PfePlre relgents ln """nce but cbcard If ~ Il.-.y sIgn cA IftC4Àllltion
Of
bioIogie,ll orowth ln the
SIOCIt bottles.. COm!TIer1HI equinlerts cA tI-. "'lIgII'â .... KCIlI:bble ..cIllitf_nt a10ek conc:enlf1Ition
m.y 'De ",S«! il lloses .-e .,.usted PfOPOl1ioM1y.
••
~
bcItrer soMlion.: omotve $.5" KH~..
21.7~ Iill(~•.
33.-41il ,....HPO.. 7~,.nd 1.7
iii NHoCIln .blIllt ~ ml dislilled • • MId diIule to 1 L The pH s/IoUld Ile 7.2 wiUIOIA fWÙ8" MljUSlment.
AltemaIlivfIl)', cf
4... Q5 Il KHPQ. or 54.3 Il I(,HPO.ln
'lritll ~ NIOH .nd dilute to 1 L
b. AlagrJN.Îl.m ~ 1OhJtiotI.
Dis$llIVe
.bol,II7lll1","~_ .
AdjUll pH 10 7.2
22.5 Il MgSO..7H;:O ln diICiIed ...Ier Md dllllllO 1 L.
1:.
ea.tiwn dobldlr MIIl.tiDn: OlSSOlYe 27.5 0
d.
~ cNoIfdIr ~.
Ol5soIYI 0.25 Il
~
ln ftiIed __ Md dlute 10 1 L
F~
ln (hliled ....... lInll dllull 10 1 L
1. Add and tIIkaII ~ lN. lof' MWl4iullon of CIUItic or .cid ~e s-mples.
1) Add _ SlDWly while stirrlng, .cid 28 ml oonc tulfllrie .cid 10 distllecl_er. 0IIUle to 1 L
2) ADuII_1JissoIve -40 1l5Odlum lI\Ôwdcle ln dIIlhd ...1....
f. SOdIiIm wtfë.autJoo: Di1«1M11.575"
N~
OlIl.rte to 1 L
ln 1llllO ml dlsmed wat~. Thl!l sokMoo III nat
stable: pœPllnt dety.
Il. Mtrî/IctJtiorI ~
2-ehIoto-6-(lrichlorometll~ ~ne.·
h, ~ fI(:id aoUiorI. Dry "'egenlll~ gllJOO5e n
ref\lelltllrtMn 01l.Un'*: edd" lOS"e
!or 111.,I,dd 150 malilkl::oslt.nd 150 ma glLUmle ~ 10 clsliled water.m ôlute 10 1 L F'fegeN frwIl
1mmel:I.."Y beloN lIMI.
~ Amrrlof*m r:NoildlllOldlcn: lJls3<We 1.15 Il NH.C11n.blIllt ~ ml. diItiI«t WllIIf,
!MIll NIOH 1OIutlDn, n
J.
dlule to 1 L Solution conI.lrtI 0.3 mg
~ust
pH 10 7.2
~
DIIlli::Wl weteI'; Use dem/l.... aIzed. dislilled, IIp, Of I\IblI'IIIwat_ for ITIIIklng semple dilutions.
"N1trtIc:alIon Ir4llblor. Formull2533. HICh Co.. lO'iela'ld. CO, or 1lI"'~
-4. Proudllft
•. PrePf/flllM ofrflutbn -'-r.P\M» dl 1 MlIlOkmll 01.,..", 3iI11n. 6IIilItlII boaIl
_Ir
elldl 01 pI\OIphlite bulfer. UllSO.. ClIC!" .nd FeQ, ~ 01
u de=1bed ln 14<:1. Test cIlutlon
..."'.
Il rte:K:i1bert ln 1411
iJO
lI'I8t
Seed dilution WlI1er, il ct • Ml,
of -.1'«1 qulllil)' ~ Il 00'1
Before use blIng rtilUIion . . . . 1Im9I~10 20.!: 3'C. S811Jn11' wîth 00
_.
botIle or br llerallrç wlth organlc.free lilIeted • . Meml\Mll)'. 110«1 in
/or waler 10
Incl Idd 1 ml..
br $I'Illking ln. PlrIiIII)' fllMl
~~
lDng lIIClUQh
bet;u,,,, SBllDIMl 'lritll DO. Protect water qullil)' b)' uMlg delln glas.swlIN, bJbinliI. 'nc1
/). 0illAIi0rt....,.,. .... y . Sourol_er 113IJ ml)' Ile SlOAKl befonl U51U 1ang.5 ~ pœpIllICl dilution
...Ier mee\$ qu.lIy c:orItrol Clil.lrIIln the dilution WIller bIInlt (1 ~. Sud'I stcnoe lT'lIY improye the quIllIy
of tome -.rel W1lt1tnl llLC lTIIIy .."". bIologIelll "rowItllO Cluse detefMnIIon ln lIlI'leB. Pi~bIy dO nat
,
Sten lA ....,-.lllllullon _ _ for monI tIIa'l 2. h .nef tdding
~
mlrMmIls. .nd buff&r unlllss diutlon
""". blMks COOIlslentlY meelqutlity control imiII;. Oiseard $toted suœ _ter il diIuIion water bIlInk
sI'lOWS more Ih.1'I 0.2 mgIl 00 deplelion ln 5 d.
c.
Gll.co~ lQd ctre<;Ir:
ll~ bVthe
PI IStICe oIlOldc1n1s Of by _
torUmlrIMed wtIh ooppet; _
WÎ\ll6\ldl
a-- Il'lII BOO lest Is • bIoaslty ilI 1'8....11. ClIn bI irIfIueneed
of. pool' IIedIl'lll material. DisIilled
- . SMds _
feII\Iwty' lnIctMl. Law ~Itt
we'_
~.re
.twlIys .re oblIinId
seeâs end ....at... p ~ dleck <IUlon ""'Ir queUty, eeed en.::ii. Il.
, end an.1ytICI1
tedwllqlle by mllkll'lll8OD...-nnwu 01'\ a rrilàunI 01150 mg ll~IMM'1....fld 150 mg glutamk; lC:.ldo\.
e" ""'ndlrd" ct1edl t:OlUlion. GlllllllSll hIs an exceptionally hlQh.fld vart.bIe œddlltlol'l ntl. llI.t when.
Is used INiIh glulamlc acid. u. œdd8llon nII.ls slabllize(J end it .simiIIl" 10 lhSII otllained wtIh l'l'IIny
rl'lUrIIciI* W1l5l1S. AIlem8lNePy, •• psr1b.ll.-YA:SlewItef œnt.... III idInCiIIlllH m8jor OClI'\SIllIellllhtl
conu1tu.. 1o the aoo.
USfl
tIK eon'lP)I.IncI ln pleot oIflellluc:oMillUlamlc Kld.
oeltrmkle the SolI 2O"C BOO of. 3
cjkJIlon oIlhell~"
•
"h.tImIc .ckl5llf1d.llni dIedlllllution
lI5ing the leC:h1'l~ lMIl'llld ln Ils 4(J.J. AdjuIl ~ flf conwnerdI' rnxwtes 10 QMI 3 no\.
lllllCOllll ancl 3
mwt gkümieacidln Nd! GGA .... boille. E'JllIUaIe dIII
as ...... ibed ln 10, Predeion
and Bies.
d.
SIl~1g;
1) SMd lIOUl'ee _ . it nec
'Il')'
to h.ave pre$Il'l' popcûIlon flf fI"'UOIllIO';SUl' tIPI"" of 0lCillizîng
the ~8deble OfQIlIiC:meuer ln !he $ARlple. Dl1meslIt ....estewtter. unc:hlOfWlllled 0l"0lM0....
undbll'lfedld 1f'IluenI, frum bIologk:aIlNaSll trutmenI pltnts, 'nc1
~
oontIln SlIIIstSdary mlaoblal
PGPolIttlon (JlIf eKample,
!IOlM
popu~. Sorne
surT~
1NllI_ ree.M-og WISl_M.
IIlImplee do nol. contIin 1 sul'litlenllftaOOill
UI'lIl'Nled ~ INllMII.lieinlll:lld \JrIUles, h1gh-lemperMlJl'e_stes.
arWiSIes wtIh eJdmne pH wIllesl. Far -.ch
, ... seecll/le dillllion WlIIerar saonple by.cdng.
PGPoll8Uol1 01 ~niIms. The prefl8rl'ld MId Is etnuent
Of
mlIlId Ilquof from a Ilk*IgbIlrNImenI
.)'Item ~ l/'Ie _ste. 'MIese suctllIlI«I it nor '~ailebll. use supernat.nI frum domestic
.....81....... Ifl. eetdlrQ at room tempel'llUl1l for et Ite$t 1 h but no Io~ !h,n 30 1\. When eII\lerrt Of
nitril'lCIdon"
lTWId ~fJom' ~"lf1lSlJTIeflllllo.- ' le used, irIlIl*on or
rec:ommenôed.
Sorne SItIlpIn n-.y COOll.1n rNIle~s not deflraded II: normal rlIIl!S by Ille ITIÎCI'OOfVllnlem5ln MttIed
cIomestIe waI_. s.Id sucIl $oimpe wIlh 11'\ ldIIlIed mIcroblal PGPolIation OIll.nId!rom the
uncllsinfeded etlllIerrt Of ml:oced Ilquar DI. blcqIcal proc:-.e ll'NIil'llllhe .......... 11'I1IM.1lMncI or suctI.
1«:My, DOlain seed l'rom th. reoeMng _ _ beIuw (jM"""~, 310 a km) Ille point
eueh seed 5OlonlI!S eI50 _
tef'IItll'lllll semple cf seIIIed
uN 1 soit _pension Of
not IV""'*'.
d~ elop ln
œ~.
WI'lIIn
IdIplld SIed in the IIbolstoo) by ClInIInucluIly
doi."......eINllllf .rw:lldd!ng emeI dilly Il\tlemests cf ....SlI. OIlllonlly
IiCIÎIfIIld sIudge,
Of a
COI'I' ,,1'CiI1 and prepel'lllon 10 oIltaIn the InlIl.el mcroblll
PGPollllion. Oe!ei"iÎ1 '" the 1I:lCist&l'lte œ, selisfeaoty popuIlltion b)' lesting Ille per!ormlll'lCe of Ille seed ln
800 .... 01'\ Ille umple. 800 v...... lhIllnc>'u$e fIIftIl tme cf
to , steMty hlgh Wllue lndIcate
.,.pl.Ition
succeuf\ll HIIlldllPllllïon.
2) Seed oonlIOl- Oetiw" .... BOO of \he seedilll maerial as 101' any othet $ample. Tl1Is Is U. SfHId
~.
From ua. v",,", of lhe teed c:ontrolend e klla..1
_I~ deI~ Med
l:ôM of \he Meding matertlll dlullon On ua. dIulIon
DO upteke.lôNItY. melœ dlutlons 01 seed lUth Ih.' Ille I.rgesl
q ~ resub
ln
.1e15l 50'" DO depletiCln. A plol of DO depletion, ln ITIlIgfwns per liler..... l'$IlS mlIIlIeIs 01 seecllor"
bolIIel hmng 1 2-mg/l 'lelllllion and Il.lkrQ/l mininun rflIdlill DO lhoUld pl
lM 1 Sll'aIghlIlne for
wIIIch the lIope indicaI.es DO depIellon pel" millier ol SMd. TIle ~ illelœpt Is axygen depIlIIIon
tI\ISoId by the dlküJn waler end shDllId Ile . . . Ih.... 0 1 ~ ft 411). Al,""""""
divlde DO depIetIon
by velume 01 $Hel in millilers for Iec/I seed coMrOI bollIe having e 2-4!9'L depletlon end e
1.G-<'r'llII\.
reslduel DO.... ~ dle resub lor el bOttleI meeting mirWnU'll depll!t10li end resIdllf,l DO Clleria. The
DO upleke alIl!.. • .... lothe seed lIdded 10 eedl bollIe $IlOI*l Ile betl"een 0.11100 1.0 mgIl. lM \he
emount of sr:ed lICldlId $IlOI*l Ile edjusted from lhk l'Inge k11t1e118:l11lfed 10 provide gJul:os&.Qlutarnic
edd cheell reslAls ln \he l'Inge of let!,j, 3O.S rr9L To dItefmlllll 00 llpllke fol" 1 lest bollle. M.IOIfIe::t 00
trom totel DO updIte (_1 S).
TeeI1niques for eddlng seedfrç mateMl te dilution WIler .re desalbed Ior lvfO . . . . <IIWoIl methods
uptelte et!1IblltIIbIll 10 the seed
('f .1).
e, .sampIe ~ Chedl: pH ol" Hmple, belore lestlng unIeM pll.h lS experlencAt Indtc.lIIU
IhM pH If; withln 1IM _ptatH l'Inge.
1)
sa'lJlIescon.eirinll ça,at;c eIkeIInIy (pH ~ 1.5) orecdty!PH '" 8.0)' Neulrelize Jamples 10 pH8.S
10 1.5 wiItl • -*'lion of IUlturtc acld (H28Oo) or 5Od1l.m IlylllOldde ~ of lUdI strength 1tIeI\he
quentily of retgllIIl doe:$ llOl djlute!he
sen1Pe by lTIO!tI thIn O."'. TM pH 01 d~utlon WllIer $hOUId nol Ile
etfected by the lowesI umple dlutlon.. ~ seed SIJIlp!el 1tIeI heve been pIoMdfuillld.
2)
8a'nPes oonteIr*lg resldual dllorlne œmpoundI-1f pl 'bill, lV'Olcl ~ lXIMelr*lg ~
dIlorInlI by samplng .hNd ol ctJIclfirl (~1
dellldeble ehlofIlllI rnlduel Il pl
pl'"
~Tt. seed
'II.
Il' Ille semple htlS beel'I eIlI<lmItecllM no
Ihe dikl:1on ",'er,1I resk\uel dlklf'o"is present. oecnlollneto
Hmple and MId lM diIllllon WIler(f./). 00 IlOllest
ctJlOf1~semplea
witIlout lIHding
!he dill1110n water. In tome ..""*,, cIlkWlne .... dlsslpaü VI'ltt*ll 10 2 h ol Sianding in 1Ile Iight. ThIl ont"
oocurs dl.ring _,.,... tn"'flOllend llendllng. For semples ln wtlIcIl et...... resil:Iual does nol dissll*e in
e ree5CMlllb/y short tîme. deltlllY cIllollne resldllal b'J' ecbIng ~ lIOkIlion. Oetetn'ène ~ YOIumt
01 N~ ~Ion Ol'I e 100. 10 10l:l1)..mL porlIoo 01 neulnIlzed semple lIy Mklilg 10 ml.. 011 + 1 - ' c
eetd or 1 + 50 H,SO.. 10 ml.. pollsslum locIde (XI) 5OlImon (10 (il00 ml) per 1000 ml.. portion, end
ItrMIng wIltI Ne,SQ, solution 10 !he SUidi>lodlne
n
poirIl for rniduel. Adcllo neutreliHd ~!he
rel.-veVOhme 01 ~soIutIon do!t&"iÎIlild by ~ lbovelest, mbl, and e/Ilf 10 10 20 n*l dIlidr.
semple lor reslduer dllortne.(NOTE: F" •
CIfl8ln
Ne~
""eltS .... 0ll)'Q8f1 dM\II'Id and mets slowIy with
orverllç eNol ..,.... toliipounds Illet mly Ile IQ$CInt in chlol1nellld _iipl",)
3) SlImple:s: eortelrq olher IlIllIc substenon· C«1eIn industriel WQtes. forell8lT1Pe. plellng w85tH,
oonteln lOIde meteb. SUctI semples olten requirtl IPtcillltudy end 1tUilmtflt.
4) ~ SlJpetSltutllted oMlh 00 -
SamPes toIUIning moN Ihan 1 mg DOi L al
eneourll-.d ln llDld wMefS or ln ...cetwl'lere
phoI~ oocu.....
2O"C rnay be
To prnent 1055 or 0X'flIl!'I1 dul1ng
lneul*ion 01' such s.amples. reduee 00 10 satul'\I6on" 2O"C II)' brlnging samPie 10 aoout 2O"C in pnaly
a.d tIIIlu...m1le
8lI~8tI~
II)' 'ofIgorous sNIUlg
.5) S8mpI8lmnpet1ltUflllIdjusIrnerI. BrrQ
Of
II)' MntifIg wilh de8n,lV1erecl eon1PlU5ed er.
_'IPM 10 20 '" l·C before rNIking 61utions.
e) NItrWIc:a1lotllnhlbtlon -. nIlrtIIc8lion inhibition it lIalfed iIdd 3 mg 2-e1llclro-6-(\riehloIo metllyl)
pyrIdlne (TCMP) 10 eldl3OO-mL boIIllI befOI'\l e.ppng Of iIdd
~ ernomls mlhe
61U1ion ~151 to
make a ~I c.onc:el'llratlon 0110 mWL (NOTE. PIA TCMP may dissoMl 5Iowly and cali noal OIlIOP orlha
sample. Sorne CGlii""dal fom1ul.IlIorts l;\i&6otve n'lOI'IIl'\Iadlly 1llA . . nollOO'1lo TCMP; acljusl. closaoe
fl!JQ\àflI nitriIIcallon ltIh1bl1on lndude, but . . nol hmiled 10, bioIoglcaly
truted tiIIuenls, Mm'" .alded wiIh bIoIoglc:.Dly!tulfld 8ftklen\$, anc:t river
NoM lhe lIM 01'
l'IIlrugan ln/ibilioI'I iI'I reporting ruuha.
M:COIdlrVY.) 5am1lle5 thaI m.y
waI'"",
(0I\tl0tI M$~: Mlk. _rII cIlullons of semple Ihet -..il fil...." ln 1 flI$ldUlil 00 or alleasll/T9'L
and. 00
U~.
01 al1eall21'1'O'\.1fter • .5--4lneub8tlol1. fMllliMIonI . . '8ClOi,".... lded UI'IIe:5I
IJlpIf!ellCl with • PlIrticUar S8ITlI)le sIlOIWs Ihl! use of • srnaIer number oIl!1ution1 procIuee. al Iell5t Iwo
bottin gImg aeœp_ble m1nmum 00 deplellon 8IId res!du81 ....... A lIlIlR rapkl.nlllysls, sucll •• cao,
may b8 00lTIl8I1Kl .,pwdl'''eIy oMlh l!IOO and serve. 'lIl1ide ln "'ldl~ dtulIons. In the absence of
pI10r knowIellge, use ltIe 1'*""10111 dilulion5: 0.0 10 1.11... for lIlJOng il'ldUSll1l111""'stes, 1 10 5" for IWW 8nd
settled ........ ewater,.510 ~~ for bioIogkaly ttPlad aflbanl, end 2S 10100'1l0 for poIUled ItYMtmllB.
P1epfIre cHutionI eiIher ln 1Ill1l1ual8Cl eytlndiarS Of lIOlumalr'oc: g\a$SWlIfII, am Iht!n trans1er to l!IOO
boftles or prePll'll dii6dl) ln BOO bo«leI. Eilher dlullOIl INUlOll çan be c;ombinld wilh eoy 00
m e ~ lechniq.....
l ~ and
WIlen
Tlle numller 01 bolIIl!:!. 10 be prepared for elCll llNtion llependl OIltlle 00
thll'll.mb8r of l'1IflIlçaln ·d!5111lll
U$I"" ~Id cyInde~ or YOlurnelItc: 18Sk$\0 PfePlIfII dlutillnt, and lfIIIleI'I M,(tiolll Il
_ l ' l , edd seed eflllerdir8ic:lly 10 dilullon Willer or 10 InllMllual
~rd8rs or
l\Is.ks berore dlllllon.
5eedlng 0I1ndiYidu8l ctloideiw or.-u awIda a deciniolg raio ol"Mell aD urJ1lIlI8$1IlauYIg dlutlone
'rI rTlICle. 'MIen dilutions 8r1 prepared dinK::IIy if'I800 boIIleI end when ~Inv b I*'n.ry, aôll seed
dlreal)' 10 cliIlAIOn WlI\eI" or dinldly 10 the l!IOO bolIleI. \Mlefl. bolIIe 00Ill81ns Il1OI1Ilhan e~ d Iht!
NmPI8 SIller dikI\ion. IlUIrienu m.y b8 1Imit., if'Iltle cliIuIed sample and $Ubsequently leduoe lliologIl;aII
actIYIy.1n sudI aemplas. edd tlle nUlI1lnt.
Ind bulrer sc*IIIonI ri 38 through al difllQl)' l<I
mlnel".
Irdri:luel BOO bon/IM al 'l'SiI.e 011 mlA. (0.33 mu 3Ol).mL boille) or lJ5e oommeru.r prepared
solutiorls dMigned 10 ÔOiM tIlIappropriale boille slza.
1) 0i1Wor'lll prel*ed Î'lg""." c:yirodeI1i
Of
vQlOOJelric I\a$k$ - !fIlle azIde modilleallon 01 tha
tlJ'rnetric:: iodomettk; .. 18tI1Od (S8dion 4500-0.C) Il uMd. carwtull)' slphon dlution Willer....lled If
naœslll')'. inIo e 1- 10 2-\. Il «l, lluk or C)'IFoIter. fil liait ru. wltr'oouI entrWnÎllll8Îr. Add deHed
quartlly 01 cal'llf~ miqd Nmple end dilule 10 aP9f'OlXlale Ievel 'IiiltI dlution l'fat«. Mill wei wiltI.
plwIgfi-I)'pII1ftoIng rad,
iIYOIll8t'llfa1nl~ air.
SIphon mîlœd 61ubon lnto Iwo BOO
bo"*. Determine Inll\al
•
1000
. l, m .
1
r r: sea •
0
d Incubate 10 5
.p on dnutlo rn ,ra nta 0
elearœl~'
,20·C. 1
BOO
aœd co tents WIIh sampi ,dnutlo
01' e'
te for 5 d at 20·0.
th
ne
OU,
dl
no bubbTes.
For d uUo
en uslng
. Calcul 1·0
G
For uctIlest boille
celculela
mea"'" Ille 2.o.nv'l
~
DO de'pIetIon .-1 Ille I.D-mgIL reslduel DO,
BClOo es lolIows:
'Nhan cIlullon w..... IlOt "I~:
0, • DO Of dlllllld .-npIe lmmedlately llfIlH" prepltetlon, rngIl,
D.l-
DO cl dlluted ..m~ aner S d lneubatlon el
2O"C, rr9'\-,
P - cIeclr'Nl vollM'llellk fnK::l1on Of 5lImpie used,
e, - 00 Of seed oon1rol betore Incubllllon, mgA.. (14d),
~.
DO cl M«I eottrol eIl~ IneubliltlDn mgA..114dl, eoo
, - ratio 01 ued ln dik.ted ~a 10 lelld in ued control- {'At ued in diklled NmPe)/("lt Mad ln &Md
""""'
'16'I"Id
~. edded
dÎlec:tIJ 10 semple «10 ued eontn>l boilles:
,. (Volume cl eMd ln dîIultd NrTll*)/(Yokme cl Mec! in ued COllltOl)
AiIpOfl r.-ullS .. CSiOO, If nitriflcetion
'_bn _
~
Inhibhd.
umple dlulion l'I'IMIS Ille CIlefla Of a residual 00 Of al 1e15l1
mg/\. end 1 00
Cle9letlol. Of li! leesl: 2 "9'l endltHtte is no ~ Of loxlcity li! ~Ilet $ample c:onoent~« lhe
exilIlence Of en otMous enomlIPf, _ age resulls ln the lIDClIlptebIe range.
ln Ihfle ceIculeIIons, do IlOt mile UM.edIoi.s for 00 upteke by lhe dIuIIon -œr Ill.nt du~ng
Ino.lbelion. TIwe COIftIdlon bi unlMlœssery • dilIAIon _ter meefs!he blInk a'ilelte SlIDUllled aboYa.
If!he
dllr..tlon wM'" doM IlOt meeI. lhe5e aùIriI, IIIOl)er UM.ections are dil'lk:ult : do IlOt reeonl resulls Of, es a
mlnlmum, merIt. \lIem as not meetirQ <pJ&lity conII'CIl eneN.
Trlef1l1s no me-m lot esl'bisIlirIg blIs Oft:lltl BOO prooedure Tllt gh- ~ ia g1l1lerrie edd c:ladt
PlellQôbed ln 1 4c Is Inlended la be • f1lfeRflDI poInl for lNeluelion cl dUIon WIter qualIty. seed
e~,
eeld 50hrtlOll
eoo
~
~
teeh.-qu.. B;ngle
1
h_1lory lesb UIinlIe
~
mtud glucose glutllmlc
Ille Iobo'Ing Itilllls:
NImber cl motlths; 14
Nwnbef clll1pl1c:;ileS: 421
"~1IlOfIltIy .eco.ary.
2lM mgIL
pap:7dcl
AYet'Ige monill/y tindalll deYI8tion: IO.4/f9\.
of ÎIUII18tlOl'IIlSlIldin,' ellCh IlMIImg 2 to 112 lllbOlaloriel (and 85 many ..,alysts and
III a _
seed !IOO~, 5-d 800 trlNSIJremenll were lMlle on synlh~ic Wlltr .."pel OOllI"ni'lg 1 1:1 l!'tI4ufe
of gIllOOMlnd gloMmle Iâd ln thl104ll concent~ renge of 3.3 10 231 mgJI.. The regresslon eqUlliDnI
for mun vllue, X; Ind
$laIldard deIIIItion,
S, from I r - Sludiel ... ~
x- 0.&.S8 (1dUed lev&\. mw'lJ. 0.280 mgIL
S- 0.100 {8dded Ievel,
For lIM! ~ milled
mwLl • 0.547 mgIL
PI1marY 5llIndard. the ....... 5-d 80D WlMl be 198 mgIl with IIlIndIIll
dtr.1111oo of 30.5 mg,IL \MIen
~
inhibitDn''' lIMd, GGA ln resuItI fallinog outIide the 188.
30.5 œnllOllnjl qUle oIlen IndJc:lle use of lncomra _!'Its 01
Ille GOA tut 10 Kt1IIMIlMIJb IlIlrG Mllin I/lÎS
a.
~ 1I1W$;
t1I,..
s.eeo. Adjust IITIOUnt of MId Idl*llo
Beeause of IN", laaors Ifredlng 900 lests ln mulIllbolMOly MuClie$1IId I/Ie
resuIllng exil" ... variabillty in telIl resulls. 0lIl standard devIItIon, as delll .. iÎiMid by Intll1ootloi'1ltol'y lestS.
le I.oonlne..dtd. 1 oontroIlimlI fat lndIvlduIlliobiHllviltts. AlIl1'llliwly, fol' .1dI1lbclrMllC'1, estlbllsh Ils
COl'W1llIImltI by petfon'nlng 1 mîntTun 01 25 gI~ut.am;ç acid c:hIcb ('( "c) __ a period of seVItIl
we.... or lTICll'Il'-lIId e..... 4l1ing Ule meln Ind slIndard ~on. usa tI'llI meln '" 3 s1andM'll deYtIlIonI
IilIllle
COI'bOI'" forfUtln lib'
' 1I11Umk: lÔIl dledlI. eœ
laboflltory lests ~ e d Ibove Ind 10 1nIl1f1aboiwlOi,
1lMl % 30_5,
c:IIlcl.Q1td COl'W1llIIrrils lo the slngIo­
00l"Ilnll1L<nl\$ Ire outslôe tfla I1Inge of
_",,*e lhe contrul ~ Ind inYeeliO'" -.rel of the probIem. ,,~~ BOO ror.
lllucose-glllllmîe Idd eheek Il Olltsbe the ao:epted oonlroI ionII range, rtIjed. tests medlI with lhIl MId
.nd dIutlon _el".
b.
~ l1IfJQl'
and dfJf«;(W)j,ImI:"ThI WOf\.II'lg raI'QIls ecp,IIoIlO Ille dirreilllœ bIt....n the
_ m Initill DO (7 10 Il mgIl) and mirlimum DO re5ldual of 1 mgII. ~ by lhe dlution fldor.'"
~ dltlCllon
limlI of 2 mgJI. Ii lSlablihld by the ~ll'" ,MIfllIor 1 mîlWl1um DO deplellon of 2
"9'1-.
7.R~n«
1. U.S. EIM_IolEHTAL P ~ N;;.ucf, CIma! 011 Rf.~ _ OEvFt.oPwiNT. 1lllM1. MdllOd by
Me\IIod ~Iœ lrom W&ef ~ ('M') l.Ibcntory Pertonnllnel EVllualkln Stldes. Quafty
Assuranee ~neh. EnvIrunmenlII Monllomg.nd Suppon Lab.. ClndnMd. OhIo.
&.
Biblioll~hy
YouNO, J.C" G."'. MeOfJlloOl'T" O.~. 1881 ....."Mlons ln 1he BOO P10e1ô1n for Ille 15th Idllion 01
SLandatd Melhods for Ille ExIn'irIatIon 01 Waler and wa!ll.lWIler. J. w.ter PoIuf. Con/n;II Fed 5'1:1253.
C6tllndllllliettlClds fortl'lll Enminallon 01 Wller and w.stewIl•• 2OU'I Ed. Amerlean Publc He.1th
.t.1IOd'll1on.1>.mIrieIn Will.... WorkI As5oclIlIon, W~ ErMroomenl FIdII1IIÎOfI.
plJtlclel
•