Quick Start

Quick Start
FLUOVIEW
FV1000
CONFOCAL LASER SCANNING
BIOLOGICAL MICROSCOPE
FV10-ASW 【Ver1.2c】
Petition
Thank you for your purchase of Olympus microscope at this time.
Prior to using this microscope, read this instruction manual for sure to utilize full-fledged
performance of this microscope and ensure customer’s safety.
In addition, read section “Safety Guide” and “Hardwarel” of User’s Manual – FLUOVIEW FV1000
as well as instruction manual for microscope to understand how to use the equipment thoroughly.
To use laser system correctly, read instruction manuals that come with each laser equipment and
light power system.
Hold this manual by your side when using this microscope all the time and keep it with care after reading.
AX7284
Caution
1. Part or whole of this software as well as manual shall not be used or duplicated without consent.
2. Contents described in this manual are subject to change without notice in future.
Registered trademark
OLYMPUS, FLUOVIEW and LSM are of our registered trademark.
㪚㪦㪥㪫㪜㪥㪫㪪
1 About this manual㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㪈
2 Software Start㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㪈
3 Window configuration㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㪈
3-1 Image acquisition and 2D View window 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪉
3-2 Image analysis and Image calculation windows 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪋
3-3 Image processing and 3D View windows 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪍
4 Basic operation procedures㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㪏
4-1 Acquiring image (XY observation) 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪏
4-2 Specifying dyeing method and setting spectral detector 䍃䍃䍃䍃䍃䍃䍃 㪈㪇
4-2-1 SPECIFYING DYEING METHOD 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪇
4-2-2 SETTING SPECTRAL DETECTOR 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪈
4-3 Acquisition of series image 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪉
4-3-1 ACQUISITION OF XYZ IMAGE 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪉
4-3-2 ACQUISITION OF XYT IMAGE 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪊
4-4 Opening file and saving file 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪋
4-4-1 OPENING FILE 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪋
4-4-2 SAVING FILE 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪋
4-5 2D View 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪍
4-5-1 DISPLAYING IN TILED WINDOW 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪍
4-5-2 DISPLAYING IN ENLARGED SIZE 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪎
4-5-3 LUT SETTING 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪏
4-6 3D View 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪈㪐
4-7 Image analysis 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪉㪇
4-7-1 ROI MEASUREMENT (ANALYSIS OF 1 FRAME) 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪉㪇
4-7-2 SERIES ANALYSIS (ANALYSIS OF 1 SERIES) 䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃䍃 㪉㪈
5 Exit of software㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㪉㪉
About this manual
1 About this manual
This manual describes method of software start, windows that can be displayed and procedure of basic
operation.
For detailed explanation of each window, see Online Help that is displayed through [Help] – [Help].
2 Software Start
1.
Double-click icon on desktop.
2.
Input [User ID] and [Password] and click <OK> button.
TIP
Software would start up.
User ID assigned to Administrator is the ID with administrative right. Unless
administrative right is given, ID assigned to other users comes with general
user right. When software is started with Administrator-right ID, it is
possible to add, delete or change information of ID’s assigned to general
users.
NOTE
ID’s displayed in [User ID] drop down list are as follows.
x
General user right ID’s
x
ID assigned to user who logged in last time
3 Window configuration
Window configuration of FV10-ASW (application software) is explained.
Windows that appear are displayed in 3-page spread.
Page
1
Window configuration / Image acquisition and 2D View window
3-1 Image acquisition and 2D View window
Buttons for image acquisition
Buttons for 2D Setting
LUT setting pane open/close
Transmitted light ON/OFF
Spectrometer setting panel open/close
Fluorescent light ON/OFF
Bleach setting panel open/close
Digital zoom
Font/color of ROI
Dye method setting
Image acquisition info panel open/close
ROI Manger
Filter path panel open/close
Thumbnail image registration
Loading acquisition condition
Image acquisition window
Window to set image
acquisition
Preview Start/Stop
Acquisition Start/Stop
<Lambda> button: Lambda series
image acquisition
<Depth> button: Z series image
acquisition
<Time> button: T series image
acquisition
Bleach Start/Stop
Acquisition
setting
Scan mode/Speed
Image acquisition
Number of pixels
Zoom/Pan/Rotate
Detection mode
Noise filter
Sequential scan
Image acquisition control panel
Laser intensity
Spectral Detector setting
Bleach setting
Lambda series setting
Start wavelength setting
Z series setting
Wavelength width setting
T series setting
Window to set bleaching
2
Page
Window to set spectral detector that
can be opened with use of
button.
Window configuration / Image acquisition and 2D View window
Buttons for 2D View
ROI tool display/nondisplay
Each channel display/nondisplay
Single display/
3 plane display/
Tiled display
Reversal replay
Start image setting
Step backward
End image setting
Step forward
Projection OFF
Zoom display (drag on image)
Normal replay
Magnification display
MAX speed/
1 per 1sec/
3 per 1 sec/
Real speed
Loop replay/
Bound replay
Tailor to window size
Window to set 2D
View
Z/
2 per 1 sec/
T/
Lambda/
Topo. Z/
Topo. Lambda
3D Display
Vertical intensity
profile of 2D
appears.
Window to display 2D View. When “Live” is
displayed, image being acquired will appear.
For 3D
Display
2D Setting
2D View
Series image setting
Tiled display setting
Projection setting
LUT setting
Intensity profile setting
Grid line setting
Horizontal intensity
profile of 2D appears.
LUT setting window to
change display color
of image.
Page
3
Window configuration / Image analysis and Image calculation windows
3-2 Image analysis and Image calculation windows
[Processing] – [Spectral Deconvolution]
Window that separates fluorescence from
multicolor dyeing specimen (spectral
deconvolution).
Spectral deconvolution (Fluorescence separation)
ROI Spectral List
Spectral Profile
Spectral DB List
Spectral information
Dye DB List
Series analysis/Live Plot
ROI Measurement
Selection of series axis
Lambda, Time, Z
ROI measurement value
Selection of display value
Average: Average
Max
: Max. Value of ROI
Min
: Min. Value of ROI
Integral : Integral of ROI
Statistical value at each row
[Analysis] – [Single] – [Measurement]
Window to execute ROI (Region Of Interest)
measurement
4
Page
[Analysis] – [Series]
This is a window to execute series image
analysis. Change of average value, integral
value and time for intensity value can be
displayed in graph. Set image applicable to
“Live” and graph can be displayed, real time.
Window configuration / Image analysis and Image calculation windows
This is a window to call
image by selecting folder
that the image is saved.
Double-click thumbnail
image so that file is
called out and displayed.
[Processing] – [Image Calculation]
This is a window to execute calculation between
images. Image intensity calculation can be
executed.
Logic calculation: AND, OR, XOR, NAND, NOR,
NOT
Explorer
Calculation between images
Image 1
Image 2
Preview
Folder selection
Ratio/Concentration
Histogram
Calculation
Image list
Ratio type setting
Thumbnail image
Baseline image selection
[Analysis] – [Single] –
[Histogram]
This is a window to display
Histogram.
Background image selection
[Processing] – [Ratio/Concentration]
1. In creates image that converts intensity
ratio between Ch1 and Ch2 to intensity
value.
2. It creates image that converts ion density
of specimen to intensity value.
This is a window to
display thumbnail and
property of the image
currently called out.
Page
5
Window configuration / Image processing and 3D View windows
3-3 Image processing and 3D View windows
Buttons for 3D Setting
[3D Setting] display/nondisplay
3D property display/nondisplay
Outline display/nondisplay
Comment display/nondisplay
Center mark display/nondisplay
Tile display setting
Scale display/nondisplay
[Processing] – [Threshold]
This is a window to binarize image.
Binarizing
2D Display
Original image
Preview
Filter process
Intensity profile display
Original image
Preview
[Processing] – [Filter setting]
This is a window to filter image.
6
Page
[Analysis] – [Single] – [Intensity Profile]
This is a window to display intensity profile inside ROI
selected.
Window configuration / Image processing and 3D View windows
Buttons for 3D View
Slice move/
Image rotation
Each channel display/nondisplay
Single display/
Tile display
X-axis rotation
Y-axis rotation
Z-axis rotation
Slice creation
XZ/
YZ/
XY/
Free/
Box slice
Slice rear display/nondisplay
Engaging tiled image
Slice fixed
Displayed image save
This is a window to set
3D View.
button opens this window. It is a window to display 3D view. Volume
rendering and bird’s eye view can be displayed.
3D Setting
3D View
Projection
display/nondisplay
Display position
of Box Slice
Animation
· Slice move
· 4D display
· Angle rotation
Online Help
Display height/Brightness
[Help] - [Help]
Online Help. See here for operation method.
Page
7
Basic operation procedures / Acquiring image (XY observation)
4 Basic operation procedures
4-1 Acquiring image (XY observation)
The figure, shown below, illustrates an example to acquire single dyeing color image of
green fluorescence (FITC).
4
5
6
1
2
3
See page 10.
5
(1)
(2)
(1) Adjustment of image brightness
HV: Sensitivity of detector is set. (1-1250V).
When it is set at 700V or more, random (dot) noise
will be increased.
Gain: Amplifier gain is set.
Offset: Background is turned darker.
(2) Adjustment of confocal aperture
Click <Auto> button and use it while it is set in
“pressed-in” state ( ).
(3)
(3) Adjustment of laser output
In case that output is set high, the ratio of fluorescent
discoloring will be increased.
Use it with the lowest value as it can possibly be set.
Procedures
(Adjust focus of object with visual observation, beforehand.)
1. Click
and turn it to “pressed-out” state ( ).
2. Click
and turn it to “pressed-out” state ( ).
3. Select fluorescent dye to be observed (see page 10).
4. Start repeat scanning. [LiveView] window will appear.
5. Adjust image.
6. Stop scanning.
8
Page
Basic operation procedures / Acquiring image (XY observation)
7
8
9
10
7. Click <Auto HV> button.
8. Select [Scan Speed].
9. Acquire image.
10. When image acquisition is completed, the image acquired will appear in [2D View –
Live Image_(x)] window.
11. Save image file. (See page 14).
TIP
Click
㩷located at upper right part of window so that scanning will be
started and appropriate HV that suits to specimen will be set.
TIP
If
is clicked, the same condition applied to the image being displayed
in [2D View] window can be set. (See page 16 - 2D Setting)
Page
9
Basic operation procedures / Specifying dyeing method and setting spectral detector
4-2 Specifying dyeing method and setting spectral detector
4-2-1 Specifying dyeing method
Select fluorescent dye that meets with specimen.
Light path to suit to the fluorescent dye selected will automatically be set.
Image acquisition
1
2
3
4
Procedures
1. Click. [DyeList] window will appear.
2. Select fluorescent dye from the list and double-click it.
3. Dye selected will appear. When redoing selection of dye, double-click the dye and
delete it.
4. Apply the setting and close the window.
10
Page
Basic operation procedures / Specifying dyeing method and setting spectral detector
4-2-2 Setting spectral detector
Set range of fluorescent wavelength that each channel acquires.
When setting is changed, it will immediately be reflected to microscope.
Image acquisition
1
2
3
4
5
7
6
Procedures
1. Click. [Spectral Setting] will appear.
2. Set start wavelength of CHS1.
3. Set wavelength width of CHS1.
4. Set start wavelength of CHS2.
5. Set wavelength width of CHS2.
6. Select filter of CH3 from drop down list.
6. Select filter of CH4 from drop down list.
7. Close window by clicking <Close> button.
Page
11
Basic operation procedures / Acquisition of series image
4-3 Acquisition of series image
4-3-1 Acquisition of XYZ image
This section describes the method to acquire the image by setting uppermost and
lowermost limit in contiguous tomography.
8
9
10
3
2
4
5
11
6
7
: 1 click 1.0um
: 1 click 0.1um
Procedures
(Adjust focus of object with visual observation, beforehand.)
1.
2.
Perform procedures 1 through 8 described in page 8.
3.
Start repeat scanning.
4.
5.
Using
6.
Input in [StepSize] and check checkbox located to its immediate left.
, bring uppermost image of specimen in focus.
When uppermost image of specimen appears, click <Set> button to determine.
Using
, bring lowermost image of specimen in focus.
7. When lowermost image of specimen appears, click <Set> button to determine.
8. Stop scanning.
9. Click and make it in “pressed-in” state.
10. Acquire image.
11. When image acquisition is completed, the button will change to
.
When it is clicked, the image acquired will appear in [2D View – Live Image_(x)]
window.
12.
12
Page
Save image file. (See page 14).
Basic operation procedures / Acquisition of series image
4-3-2 Acquisition of XYT Image
This section describes procedures from XYT image acquisition to save method.
5
6
7
2
3
4
Procedures
(Adjust focus of object with visual observation, beforehand.)
1.
2.
3.
Perform procedure 1 through 8 described in page 8.
4.
5.
6.
Click [Time View] window and check scanning condition.
7.
When image acquisition is completed, the button will change to
Input time interval for scanning in [Interval].
Input number of images to be scanned in [Num].
Click and make it in “pressed-in” state.
Acquire image.
. When it
is clicked, the image acquired will appear in [2D View – Live Image_(x)] window.
8.
Save image file. (See page 14).
Page
13
Basic operation procedures / Opening file and saving file
4-4 Opening file and saving file
4-4-1 Opening file
1
Procedures
1. Select folder where image exists with Explorer.
2. Double-click the file to open.
Image will appear in [2D View] window.
2
4-4-2 Saving file
In case that image file is saved
Procedures
1
1.
Click mouse right button over thumbnail image that
appears in [Data Manager] window and select “Save
As”.
[Save As] window will appear.
2. Select folder where the file should be saved.
3. Input file name in “File Name” and select file format to
2
3
save the file from “Save as type”.
4. Click <Save> button so that the image is saved.
4
One Point!
Formats that files can be saved are as follows.
· OIF(*.oif)/OIB(*.oib)
File formats dedicated for microscope.
· Bitmap(*.bmp)
· JPEG(*.jpg, *.jpeg)
· TIFF(*.tif, *.tiff)
14
Page
Basic operation procedures / Opening file and saving file
In case that the image is saved in the same state as “displayed” in BMP
Procedures
1.
2.
Click mouse right button over the image.
Select “Save Display” and assign name and save.
In case that animation image is converted to AVI format and saved
Procedures
1.
2.
Click mouse right button over the image.
Select “Save As AVI” and assign name and save.
Page
15
Basic operation procedures / 2D View
4-5 2D View
4-5-1 Displaying in tiled window
Series image can be displayed in tiled window.
2D setting
2
3
4
5
6
7
Procedures
1.
Open XYZ image as described in procedures for “Opening file” in page 14.
2.
Click and select
3.
Click and bring 2D Setting window to appear.
4.
Define how many rows and columns the tiled display should appear with click and
.
drag action of the cell.
5.
Select what should be used as axis for row and column.
selected is any one of “Ch”, “Z”, “T” or “Lambda”.
The axis that can be
(In case of XYZ image, “Z”only
can be used as axis.)
6.
Click to update the setting.
(7. In case that projection image is displayed together, check the box and perform
procedure 6.)
16
Page
Basic operation procedures / 2D View
4-5-2 Displaying in enlarged size
Image, tile displayed in page 16, is shown below as an example.
1
2D setting
4
2
3
Procedures
1.
Click this button and make it in “pressed-in” state.
2.
Drag the place over the image from upper left side to lower right side where
enlargement is required.
3.
4.
When enlarged place is moved up, down, left or right, slide the mouse.
When scrolling tiled display, slide this button.
Page
17
Basic operation procedures / 2D View
4-5-3 LUT setting
Color of each image channel can be set to the desired color.
2D setting
Select channel to be set
Set color for
channel selected
Select intensity range to be set
Set gamma value
Select R, G or B
Set intensity
for R, G or B selected
Select contrast for
R, G or B selected
Close window
Save setting
Load setting
18
Page
Return to default
setting
Basic operation procedures / 3D View
4-6 3D View
The illustration, as shown below, is an example of 3D View operation.
2D View
1
2
6
5
3
4
Procedures
1.
Click here.
2. Click and select
.
3. Turn to “pressed-in” state and link four (4) images.
4. Select
and drag mouse over the image so that the image will rotate.
5. Keep pressing so that animation image that turns around center of each axis (X, Y
and Z) will appear.
6.
When clicked, the image can be displayed as 2D image.
Page
19
Basic operation procedures / Image analysis
4-7 Image analysis
4-7-1 ROI measurement (Analysis of 1 frame)
Perimeter length of ROI and intensity average inside the image, etc. can be measured.
Procedures
1. Open a file as described in page 14 – “Opening
2
file”.
3
2. Click
and bring ROI tool bar to appear and
then, select ROI to draw.
3. Draw ROI over the image.
4. Select [Analysis] – [Single] – [Measurement] from
menu bar.
4
5.
Click and indicate all measurement results of
ROI.
6. Save measurement results in CSV format.
5
Measurement value of
Each ROI
Measurement value of
each ROI
6
20
Page
Basic operation procedures / Image analysis
4-7-2 Series analysis (Analysis of 1 series)
Average intensity value and max. intensity value, etc. inside ROI can be measured and
displayed in each frame respectively.
Procedures
2
1. Open a file as described in page 14 – “Opening file”.
3
2. Click
and bring ROI tool bar to appear and select
ROI to draw.
3. Draw ROI over the image.
4. Select [Analysis] – [Series] from menu bar.
5. Select horizontal axis of graph (T, Z or Lambda)
6. Select vertical axis of graph.
Average: Average intensity value of each frame
Integration: Integration intensity value of each frame
Max:
Max. intensity value of each frame
Min:
Min. intensity value of each frame
7. Graph will appear.
8. Measurement result can be saved as image file or data
4
file.
5
6
7
8
Page
21
Exit of software
5 Exit of software
Procedures
1. Select [File] – [Exit] from menu bar.
2. Click <OK> button.
3. Dialog box will appear, asking whether
the image
should be saved or not.
Yes:
Image applicable is saved.
Yes All: All images are saved.
No:
Image applicable is not saved.
No All: All images are not saved.
1
4. File is saved with name attached.
When all saves are completed, the software will
2
exit.
3
4
22
Page
OLYMPUS CORPORATION
2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan
OLYMPUS EUROPA GMBH
Postfach 10 49 08, 20034, Hamburg, Germany
OLYMPUS AMERICA INC.
2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A.
OLYMPUS SINGAPORE PTE LTD.
491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373
OLYMPUS UK LTD.
2-8 Honduras Street, London EC1Y OTX, United Kingdom.
OLYMPUS AUSTRALIA PTY. LTD.
31 Gilby Road, Mt. Waverley, VIC 3149, Melbourne, Australia.
OLYMPUS LATIN AMERICA, INC.
6100 Blue Lagoon Drive, Suite 390 Miami, FL 33126-2087, U.S.A.
This publication is printed on recycled paper.
Printed in Japan 2004 07 1.3