10825 APT Agar (All Purpose Tween Agar) - Sigma

G9663 Glucose Yeast Peptone Agar
Glucose Yeast Peptone Agar is recommended for the isolation of yeasts from soil specimens. This is a
highly nutritious medium, which may be use for other fastidious organisms.
Composition:
Ingredients
Peptic Digest of Animal Tissue
Yeast Extract
Dextrose
Agar
Final pH (at 25 °C) 7.0 ± 0.2
Grams/Litre
10.0
5.0
20.0
15.0
Store prepared media below 8°C, protected from direct light. Store dehydrated powder in a dry place in
tightly-sealed containers at 2-25°C.
Appearance:
Light yellow colored homogeneous, free flowing powder.
Gelling:
Firm
Color and Clarity:
Light to medium amber colored, slightly opalescent gel forms in petri plates
Directions:
Suspend 50 g of Glucose Yeast Peptone Agar in 1000 ml of distilled water. Heat to boiling to dissolve the
medium completely. Sterilize by autoclaving at 15 lbs. (121°C) for 15 minutes.
Principle and Interpretation:
The peptic digest of animal tissue provides nitrogenous nutrients, especially amino acids and peptides.
The yeast extract provides Vitamin B complex. Dextrose is a readily available source of energy and a
good carbohydrate source for yeasts.
Cultural characteristics after 18-24 hours at 35-37°C.
Organisms (ATCC)
Saccharomyces cerevisiae (9763)
Growth
+++
References:
1. Subba Rao, N.S., (1977). Soil Microorganisms and Plant Growth. Oxford and IBM Publishing.
New Delhi, India.
2. American Type Culture Collection, Manassas, Va., USA.
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