EATLAS DATA CATALOGUE (AIMS)

EATLAS DATA CATALOGUE (AIMS)
Acute toxicity of photosystem II herbicides to tropical
seagrass 2013. (NERP TE 4.2, AIMS and JCU)
Métadonnées | Métadonnées (XML)
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Titre
Acute toxicity of photosystem II herbicides to tropical seagrass 2013. (NERP TE 4.2, AIMS
and JCU)
Date
2015-09-08
Type de date
Publication
Résumé
This dataset shows the measured response of the photosystems of seagrasses to herbicides
in experiments conducted in 2012-2013. The data is provided as a multi-sheet spreadsheet.
The aim of this study was to apply standard ecotoxicology protocols to quantify the
concentrations of four priority PSII herbicides that inhibit photochemistry by 10, 20 and 50%
(IC10, IC20 and IC50) over 72 hrs in two common seagrass species from the GBR lagoon.
This data will enable improved assessment of the risks posed by PSII herbicides to tropical
seagrass for both regulatory purposes and for comparison with other taxa.
Methods:
Four seagrass species (H. uninervis, C. rotundata, T. hemprichii and Z. muelleri) were used
in preliminary studies to determine the time taken for PSII herbicides to affect photosynthesis,
while more detailed ecotoxicology studies were undertaken with two of these species (H.
uninervis and Z. muelleri). H. uninervis, C. rotundata and T. hemprichii were collected
from intertidal seagrass meadows (<1 m) from Cockle Bay, Magnetic Island (19°10.88’ S,
146°50.63’ E) while Z. muelleri was collected from Pelican Banks, Gladstone, Australia
(23°46.005’ S, 151°18.052’ E).
Halodule uninervis, Cymodocea rotundata Ascherson (Cymodoceaceae) and Thalassia
hemprichii Ascherson (Hydrocharitaceae) are tropical seagrass species widely distributed
throughout the Indo-West Pacific while Zostera muelleri Irmisch ex Ascherson (Zosteraceae),
(syn Zostera capricorni) is a tropical to temperate species found in Australia and New
Zealand.
Potted seagrasses were exposed to dissolved herbicides in static-replacement seawater (24 h
water changes).
All experiments were conducted under 273 ± 17 µmol photons m-2 s-1 (12h light:dark
photoperiods, Aqua Illumination LED). This light intensity was chosen as the median daily
irradiance at the Magnetic Island collection site. The glass aquaria were placed into water
baths and maintained at 25.8 ± 0.3°C (range), equivalent to the annual average temperature
in the GBR.
Herbicide concentrations ranged from 0.1 to 300 µg herbicide l-1 depending on the potency
of the herbicide. Duplicate tanks were used for each herbicide concentration and each tank
contained individually potted seagrass plant of each species.
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Inhibition of photosynthesis was measured after 72 h exposure using pulse amplitude
modulation (PAM) fluorometry. Two parameters were measured (effective quantum yield,
deltaF/F’m and maximum quantum yield, Fv/Fm). The inhibition of photosynthetic yields
relative to controls were plotted as dose-response curves by fitting inhibition data with
measured concentrations using a 4 parameter logistic model (SigmaPlot 11). The herbicide
inhibition concentrations (ICxx) that inhibited deltaF/F’m and Fv/Fm by 10, 20 and 50% (IC10,
IC20 and IC50, respectively) were determined from each curve.
Initially a series of pilot studies were performed to measure the time it takes for the four PSII
herbicides to illicit 90% steady state (maximum) inhibition of effective quantum yield (deltaF/
F’m) in Z. muelleri at single herbicide concentrations. These findings were used to ensure that
the exposure duration of later dose-response curves was sufficient. The nominal herbicide
concentrations used were 10 µg l-1 Diuron, 50 µg l-1 Atrazine, 10 µg l-1 Hexazinone and 400
µg l-1 Tebuthiuron. This data can be found in the “Kinetics” worksheet.
We also exposed all four species of seagrass to 10 µg l-1 Diuron to examine the consistency
of response times between species. Inhibition of deltaF/F’m by the herbicides compared with
carrier controls were conducted at multiple times up to 24 h. This data can be found in the “4
seagrasses at Diuron 10ug_L” worksheet.
Further information can be found in this publication:
Flores F, Collier CJ, Mercurio P, Negri AP (2013) Phytotoxicity of Four Photosystem
II Herbicides to Tropical Seagrasses. PLoS ONE 8(9): e75798. doi: 10.1371/
journal.pone.0075798
Format:
Acute_seagrass_toxicity_data_(Flores_et_al_2013).xlsx is an Excel SpreadSheet containing
multiple worksheets.
Each sheet has a description such as this:
- Tank Rep: tank replicate (two tanks A and B)
- deltaF/F’m: effective quantum (light adapted) yield measured by a Pulse Amplitude
Modulated (PAM) fluorometer
- Fv/Fm: maximum quantum (dark adapted) yield measured by a Pulse Amplitude Modulated
(PAM) fluorometer
- Solvent cont: no herbicide but contains less than 0.03% v/v ethanol carrier as per the
treatments
- Halodule uninervis: a seagrass species
- Zostera muelleri: a seagrass species
- Thalassia hemprichii: a seagrass species
- Halophila ovalis: a seagrass species
- 72 h: the duration of the herbicide exposure in some experiments
- Time (hr): duration of exposure in hours
Langue des métadonnées
eng
Jeu de caractère
Utf8
Type de la ressource
Jeu de données
Ressource en ligne
Adresse Internet
http://eatlas.org.au/data/uuid/e1f65eed-5d30-413c-b70c-af85d776d25b
Protocole
WWW:LINK-1.0-http--metadata-URL
Adresse Internet
http://eatlas.org.au/nerp-te/gbr-aims-pesticide-effects-4-2
Protocole
WWW:LINK-1.0-http--related
Adresse Internet
http://eatlas.org.au/pydio/data/public/nerp-te-4-2_aims_herbicide-toxicity-to-seagrass_2013zip.php
Protocole
WWW:LINK-1.0-http--downloaddata
Adresse Internet
http://dx.doi.org/10.1371/journal.pone.0075798
Protocole
WWW:LINK-1.0-http--link
Contact pour la ressource
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Nom de la personne
Negri, Andrew, Dr
Nom de l'organisation
Australian Institute of Marine Science (AIMS)
Rôle
Point de contact
Catégorie ISO
Biologie, faune et flore
Mot Clé
Mot Clé
marine
Mot Clé
seagrass
Mot Clé
Diuron
Mot Clé
Atrazine
Mot Clé
Hexazinone
Mot Clé
Tebuthiuron
Mot Clé
herbicide
Type de mot clé
Thème
Etendue
Description
Australian Institute of Marine Science (AIMS)
Description
Pelican Banks, Gladstone, Australia
Collection site for: Z. muelleri
Description
Cockle Bay, Magnetic Island, Australia
Collection site for: H. uninervis, C. rotundata and T. hemprichii
Emprise géographique
Ouest
147.05599
Est
147.05599
Sud
-19.27229
Nord
-19.27229
Ouest
151.3009
Est
151.3009
Sud
-23.76675
Nord
-23.76675
Ouest
146.8438
Est
146.8438
Sud
-19.18133
Nord
-19.18133
Identifiant de la fiche
e1f65eed-5d30-413c-b70c-af85d776d25b
Langue des métadonnées
eng
Jeu de caractère
Utf8
Contact
Nom de la personne
eAtlas Data Manager
Nom de l'organisation
Australian Institute of Marine Science (AIMS)
Rôle
metadataContact
Date des métadonnées
2015-09-17T19:44:13
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