High Fat Diet avant et durant la gestation : Répercussions sur le
Transcription
High Fat Diet avant et durant la gestation : Répercussions sur le
Obesity induced by High Fat Diet before and during gestation: evaluation of maternal and offspring repercussions on metabolics and oxidative parameters. DAL S. 1, Ratheau L2,3, Seyfritz E1, Bietiger W1, Pinget M2,3, Jeandidier N2,3, Sigrist S1. 1. 2. Centre Européen d’Etude du Diabète (CEED), Strasbourg, France Service d’Endocrinologie, Diabète, Maladies Métaboliques, Hôpitaux Universitaires de Strasbourg, Strasbourg, France. 3. Université de Strasbourg, Strasbourg, France. [email protected] Objectives: The “metabolic imprinting” reflects the consequences of the intrauterine environment on the future metabolism of offspring, contributing to the development of obesity and gestational diabetes mellitus (GDM). Oxidative stress (OS) could play a role. High fat diet (HFD)-induced obesity in female’s rats was used to evaluate the metabolic and oxidative effects in the mother and offspring. Methods: Fourteen female Wistar rats (200g) fed Normal diet (ND) or HFD during 3 months before/during gestation and ND after birth. Metabolic parameters (weight, fasting glycaemia, insulin-resistance), plasmatic (total antioxidante capacity, CAOT) and tissular oxidative parameters were assessed on mothers and offspring. Results: 3 months of HFD induced insulin-resistance without fasting glycemia in mothers. During gestation, metabolic parameters don’t differ; increasing CAOT observed in ND mothers was abolished in HFD mothers. Gestation induced-global OS, notably in placenta, continue after birth in HFD mothers (pancreas). In offspring, weight gain was equal; glycemia was significantly lower at weaning in HFD offspring but tended to increase at 6 weeks and HFD offspring have pancreatic OS. Discussion: HFD induced obesity and hyperinsulinemia in females. Compensatory mechanisms involved in gestation-induced OS are failing in HFD mothers. Increase of CAOT is necessary to abolished pancreatic OS after birth. OS in mothers and HFD-offspring raises the risk of subsequent metabolic disorders.