High Fat Diet avant et durant la gestation : Répercussions sur le
Obesity induced by High Fat Diet before and during gestation: evaluation of maternal
and offspring repercussions on metabolics and oxidative parameters.
DAL S. 1, Ratheau L2,3, Seyfritz E1, Bietiger W1, Pinget M2,3, Jeandidier N2,3, Sigrist S1.
Centre Européen d’Etude du Diabète (CEED), Strasbourg, France
Service d’Endocrinologie, Diabète, Maladies Métaboliques, Hôpitaux Universitaires de
Strasbourg, Strasbourg, France.
Université de Strasbourg, Strasbourg, France.
Objectives: The “metabolic imprinting” reflects the consequences of the intrauterine
environment on the future metabolism of offspring, contributing to the development of
obesity and gestational diabetes mellitus (GDM). Oxidative stress (OS) could play a role.
High fat diet (HFD)-induced obesity in female’s rats was used to evaluate the metabolic and
oxidative effects in the mother and offspring.
Methods: Fourteen female Wistar rats (200g) fed Normal diet (ND) or HFD during 3 months
before/during gestation and ND after birth. Metabolic parameters (weight, fasting glycaemia,
insulin-resistance), plasmatic (total antioxidante capacity, CAOT) and tissular oxidative
parameters were assessed on mothers and offspring.
Results: 3 months of HFD induced insulin-resistance without fasting glycemia in mothers.
During gestation, metabolic parameters don’t differ; increasing CAOT observed in ND
mothers was abolished in HFD mothers. Gestation induced-global OS, notably in placenta,
continue after birth in HFD mothers (pancreas). In offspring, weight gain was equal; glycemia
was significantly lower at weaning in HFD offspring but tended to increase at 6 weeks and
HFD offspring have pancreatic OS.
Discussion: HFD induced obesity and hyperinsulinemia in females. Compensatory
mechanisms involved in gestation-induced OS are failing in HFD mothers. Increase of CAOT
is necessary to abolished pancreatic OS after birth. OS in mothers and HFD-offspring raises
the risk of subsequent metabolic disorders.