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Institut de Génétique et Développement de Rennes
UMR 6061, Équipe « Génétique du chien »
2 av du Pr Léon Bernard 35043 Rennes Cedex - FRANCE
Tél : 02 23 23 45 09
Fax : 02 23 23 44 78
Mail : [email protected]
Réservé au
CNRS
Internet web site : http://dog-genetics.genouest.org/
Search of the genetic causes of Footpad Hyperkeratosis
As part of the research studies we conduct on the genetic causes of Footpad Hyperkeratosis
in the Dogue de Bordeaux, we would like to collect :
- blood samples : 5 ml EDTA tube (purple cap). Mix well by inversion to prevent clotting
of blood. Indicate on the tube : the name and breed of dog.
- the pedigree and the tattoo or chip if possible
- a clinical questionnaire provided by us
And if the dog is sick :
-
biopsies of pads (and nose if possible) affected and normal tissues (to be collected
at excision or biopsy or very soon after the death of the dog within 15 minutes) in the
solutions provided.
In 2 separate tubes:
- removal of affected tissue : cut the sample into two smaller fragments. Put the first in the tube
containing RNAlater solution, and the second as in formalin for histological analysis.
- removal of healthy tissue (same organ but healthy) : idem in the tube containing RNAlater
solution and in formalin.
The volume of tissue must correspond to 1/10th of the volume of the solution. Use gloves for
protection from RNA later solution and formalin. Label each tube : the type of sample (tissue,
tumor or healthy), the dog's name and date.
Once the samples taken, put at 4 ° C and quickly send by mail at room temperature except
weekends and holidays. Send at
Dr. Catherine André
Institut de Génétique et Développement de Rennes
UMR 6061, Équipe « Génétique du chien »
2 avenue du Professeur Léon Bernard
35043 Rennes Cedex - FRANCE
The samples sent to us anonymously come in the framework of a comprehensive study.
There will be no individual results. We thank you warmly for your cooperation. This stage of
recruitment is essential to the success of this research. So feel free to contact us if you know an
other case.
Dr Catherine ANDRE
Indice C (13/12/10)