S1 Fig

A
B
1,000
157
157
108
Sc
gr
146
fl
ILs
Fe
yl
ILs
Sc
gr
Cu
107
132
136
1
Sc
ILs
Sc
gr
ILs
yl
Sc
ILs
fl
Sc
gr
107
Mn
107
128
128
102
100
10
ILs
100
10
fl
107
1,000
Sc
Sc
fl
102
137
ILs
ILs
yl
D
136
Sc
Sc
124
108
ILs
107
10
ILs
124
Sc
1
E
143
102
114
123
ILs
yl
100
10
Sc
132
0.0
136
1
fl
Sc
G
gr
Mg
108
10.0
ILs
100.0
Ca
150
yl
Sc
115
ILs
108
Sc
150
ILs
F
131
ILs
Sc
yl
ILs
Sc
fl
ILs
Sc
gr
148
0.1
145
1.0
128
134
1.0
112
10.0
107
concentration
(µg g-1 dry biomass)
115
115
0.1
C
concentration
(µg g-1 dry biomass)
Zn
100
ILs
concentration
(mg g-1 dry biomass)
Cd
108
1.0
128
concentration
(µg g-1 dry biomass)
10.0
0.1
ILs
Sc
yl
ILs
Sc
fl
ILs
Sc
gr
S1 Fig. Element accumulation in tissues of barley introgression lines by comparison to the
recurrent parent Scarlett. Shown are summarizing boxplots of (A) Cd, (B) Zn, (C) Fe, (D) Cu,
(E) Mn, (F) Mg, and (G) Ca concentrations measured by ICP-AES in young leaf (yl), flag leaf
(fl) and grains (gr) of all measurements of 54 introgression lines (ILs) and all replicate
individuals of the recurrent parent Scarlett (Sc). Diamonds show arithmetic means, boxes show
the 25-to-50-to-75 percentiles, whiskers show the 10-to-90 percentiles and ‘x’ symbols show
the minimum and maximum measurements (n = 3 to 7 replicate individuals for each of 54 ILs;
n = 144 for recurrent parent Scarlett). Circes to the left and right of the IL data show the median
± 25-to-75%-tile of the single IL exhibiting the lowest and highest median (n = 3 to 7),
respectively, with the number identifying the corresponding IL given adjacent to each symbol.
Note that medians and percentiles of individual ILs were calculated from raw data without
accounting for positional effects, different from least-square means presented elsewhere. Plants
were cultivated in a greenhouse on soil collected in a heavy-metal contaminated agricultural
field, with harvest of yl at the five-leaf stage and harvest of fl and gr at maturity. Data are from
the same experiment as shown in Fig 1.