GE_DF_MidGee HoopCF_B

GE Healthcare
Data file 18-1168-04 AC
Title subhead
MidGee and MidGee Hoop
cross flow cartridges
MidGee™ and MidGee Hoop cross flow cartridges are
designed for small scale processing, rapid laboratory
concentration and/or diafiltration of critical biological solution
volumes up to 200 ml with convenience and speed
impossible to achieve using stirred cells or dialysis tubing.
These disposable hollow fiber cartridges minimize membrane
polarization due to the “sweeping action” generated by a
recirculation pump. Data derived from tests with these
cartridges can be used to linearly scale up to larger cartridge
and system designs or for scale down process optimization
and troubleshooting experiments.
Two MidGee Hoop cartridge path lengths are offered that
correspond to the lengths of Amersham Biosciences
pilot/production scale cartridges. Within those two lengths,
there is a complete series of MidGee cartridges to match
laboratory, pilot and production scale designs. The uniform
flow path design from laboratory to production scale makes
hollow fibers the most attractive of the various cross flow
configurations for linear scale-up.
Figure 1. MidGee Hoop cross flow cartridges are available in two sizes to
match the path lengths of GE Healthcare pilot and production scale hollow
fiber cartridges. MidGee Hoops are perfect for scale-down trials.
MidGee cartridges are optimized for use in our compact
MidJet™ Cross Flow Filtration System, which includes a
miniature peristaltic pump with exchangeable saddles to
accommodate size 14 and 16 tubing; reservoirs for retentate,
diafiltrate and permeate; precision backpressure control
valve; and a convenient platform for mounting the cartridge
and pump with recesses to accommodate three reservoirs.
An advanced MidJet system with two pressure transducers
and digital panel meters is also available. Low system holdup volumes allow concentration to volumes as small as 2-5
ml. Autoclavable reservoirs and a three-port (feed, retentate,
permeate) pressure transducer with a temperature probe are
optional items.
Typical applications
MidGee hollow-fiber cartridges are available in a wide range
of ultrafiltration and microfiltration pore sizes for a wide
variety of laboratory applications. When used with a MidJet
system for precise control of recirculation rate and pressure,
MidGee cross flow cartridges assure high product recoveries
with minimal shear denaturation.
• Cell harvesting/clarification
• Monoclonal antibody clarification/concentration
• Plasmid concentration/diafiltration
• Protein concentration
• Suspended solids removal
• Vaccine concentration/purification
• Virus concentration/purification/removal
• Bacteria/pyrogen removal
• Plasma purification/concentration
• Buffer depyrogenation
• Colloidal suspension concentration/diafiltration
• Continuous buffer exchange
• Continuous cell culture perfusion
• Hybridoma cell culture clarification
• Liposome purification
• Lysate clarification
• Mammalian cell clarification
Figure 2. Basic MidJet System shown with Hoop cartridge.
2
Data File 18-1168-04 AC
MidGee cartridge features
MidGee cartridge specifications
• Rapid concentration and/or diafiltration of critical
biological solutions and suspensions
Diameter:
Length:
Endfitting connections:
Permeate connection:
0.3 cm (0.125 in.)
30.8 cm (12.125 in.)
Male Luer-Lok
Male Luer-Lok
Membrane area (nominal):
16-26 cm2 (2.325 - 4.03 in2)
Hold-up volume (nominal):
Lumen side:
Shell side:
0.25 ml
0.25 ml
• Maximum product recovery due to cross flow design
• Sealed system permits convenient, continuous
“hands free” diafiltration
• Low hold-up volume for concentration to 2 ml to 5 ml
(cartridge hold-up volume 0.5 ml)
• Quick, convenient Luer-Lok connections
• Attaches to syringe for easy removal of retentate
• UF pore sizes from 1,000 to 750,000 NMWC
Autoclavable:
Materials of Construction:
Hollow Fibers:
Housing Components:
Reservoir:
• MF pore sizes from 0.1 to 0.65 micron
• Membrane area ranges from 16 to 26 cm2 depending
on lumen ID
Reservoir fittings/tubing:
All except 1,000 NMWC
models
Polysulfone
Polysulfone/Polycarbonate
Polystyrene (standard
Polycarbonate (autoclavable)
PVC, nylon, silicone
• Fits MidJet Labscale Membrane Separations System
MidGee Hoop cartridge additional
features
• Ultra-compact design
• 60 cm and 110 cm path lengths match
pilot/production scale designs
• Autoclavable
• High product recoveries with minimal shear
denaturation
• No “wall effects” to distort scale-up projections as
with plate and frame devices
• Replaces stirred cells and dialysis tubing for
laboratory separations
Data File 18-1168-04 AC
3
MidGee cartridge selection
Hoop scale-up comparison
Ultrafiltration
Membrane Fiber Inner
Diameter
Nominal Molecular
Weight Cutoff
0.5 mm
0.5 mm
0.25, 0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
1 mm
1,000
3,000
10,000
30,000
50,000
100,000
300,000
500,000
750,000
Microfiltration
Membrane Fiber Inner
Diameter
Pore
Size
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
0.75 mm
0.1 micron
0.2 micron
0.45 micron
0.65 micron
MidGee cartridges are packaged in quantities of 6, 12 and 24. Single units are
available for trial orders.
Hoop cartridge specifications
Diameter:
Endfitting connections:
Permeate connections:
0.3 cm (0.125-in)
Male Luer-Lok
Male Luer-Lok
Hold-up volume (nominal):
60 cm model:
0.5 to 1.0 ml each
(lumen & shell side)
110 cm model:
0.6 to 20 ml each
(lumen & shell side)
Materials of Construction:
Hollow fibers:
Shell:
Luer Lok:
4
Data File 18-1168-04 AC
Polysulfone
Polysulfone
Polycarbonate
Hoop cartridges are provided in nominal 60 cm and 110 cm
path lengths to match the path lengths of Amersham
Biosciences pilot and process scale hollow fiber cartridges.
The following charts are provided as a comparison of the
membrane areas for scale-up cartridges with equivalent path
lengths.
Nominal 60 cm (2 ft) path length
Housing size
0.5 mm ID
Area
0.75 mm ID 1 mm ID
H22
H24
4X2
6
9
55
65
152
—
42 cm2
0.14 m2
0.48 m2
1.15 m2
3.25 m2
6.1 m2
13 m2
29 cm2
—
—
0.37 m2
0.93 m2
2.5 m2
—
—
38 cm2
—
0.085 m2
0.28 m2
0.84 m2
2.1 m2
4.4 m2
9.5 m2
Nominal 110 cm (4 ft) path length
Housing size
Area
0.5 mm ID 1 mm ID
H42
75
85
154
41 cm2
6 m2
13 m2
28 m2
73 cm2
3.7 m2
9.0 m2
20 m2
Hoop cartridge ordering information
Ultrafiltration
Code
number
Model
number*
Membrane fiber
inner diameter
NMWC‡
4101-00
56-4101-06
56-4100-88
56-4101-07
UFP-3-C-H24LA
UFP-3-C-H42LA
UFP-3-E-H22LA
UFP-3-E-H42LA
0.5 mm
0.5 mm
1 mm
1 mm
3,000
3,000
3,000
3,000
56-4101-01
56-4101-08
56-4100-89
56-4101-09
UFP-10-C-H24LA
UFP-10-C-H42LA
UFP-10-E-H22LA
UFP-10-E-H42LA
0.5 mm
0.5 mm
1 mm
1 mm
10,000
10,000
10,000
10,000
56-4101-02
56-4101-10
56-4100-90
56-4101-11
UFP-30-C-H24LA
UFP-30-C-H42LA
UFP-30-E-H22LA
UFP-30-E-H42LA
0.5 mm
0.5 mm
1 mm
1 mm
30.000
30,000
30,000
30,000
56-4101-03
56-4101-13
56-4100-92
56-4101-14
UFP-100-C-H24LA
UFP-100-C-H42LA
UFP-100-E-H22LA
UFP-100-E-H42LA
0.5 mm
0.5 mm
1 mm
1 mm
100,000
100,000
100,000
100,000
56-4101-05
56-4101-17
56-4100-94
56-4101-18
UFP-500-C-H24LA
UFP-500-C-H42LA
UFP-500-E-H22LA
UFP-500-E-H42LA
0.5 mm
0.5 mm
1 mm
1 mm
500,000
500,000
500,000
500,000
‡Nominal molecular weight cutoff
Microfiltration
Code
number
Model
number*
Membrane fiber
inner diameter
Pore
size
56-4100-96
56-4100-97
56-4100-98
56-4100-99
CFP-1-E-H22LA
CFP-2-E-H22LA
CFP-4-E-H22LA
CFP-6-D-H22LA
1 mm
1 mm
1 mm
0.75 mm
0.1μ
0.2μ
0.45μ
0.65μ
*Legend:
H22 = 60 cm path length/2 fibers
H24 = 60 cm path length/4 fibers
H42 = 110 cm path length/2 fibers
Data File 18-1168-04 AC
5
MidGee cartridge accessories
Code
number
Model
number
Description
56-4105-75
RBFL-1
Female Luer to 1/8-in barb, nylon, pkg of 10
56-4105-76
RBFL-2
Female Luer to 3/32-in barb, nylon, pkg of 10
56-4105-77
RBFL-ML
Female Luer to male Luer adaptor, nylon, pkg of 10
56-4105-78
RBFL-SC
Female Luer to solid cap, nylon, pkg of 10
56-4105-79
KMDG-1
MidGee Starter Kit (use with PRP-07WM pump)
56-4105-82
KMDG-2
MidJet System Accessory Kit
56-4105-83
KMRV-030
MidGee Reservoir Kit, 30ml capacity, polystyrene
56-4105-84
KMRV-120
MidGee Reservoir Kit, 120ml capacity, polystyrene
56-4105-86
KMDG-175R01A
Autoclavable MidGee Reservoir Kit**
56-4105-87
KMDG-175R02A
Autoclavable MidGee Reservoir Replacement Kit**
56-4105-88
VT-06
Precision backpressure valve, nylon, size 14 & 16 tubing
56-4105-16
PTSL02-10
Pump tubing, Size 14, silicone, 3.1 m (10 ft)
56-4105-17
PTSL03-10
Pump tubing, Size 16, silicone, 3.1 m (10 ft)
**See description of MidJet
6
Data File 18-1168-04 AC
Ordering information
MidJet system and accessories
Code No.
Model No.
Description
56-4106-10
PRT-DPM-2
Two Pressure Transducers with Digital Panel Meter Readout
56-4106-11
PRT-DPM-3T
Three Pressure Transducers and temperature probe with Digital
Panel Meter Readout
56-4106-16
PTSL02-10
Recirculation Pump Tubing, silicone, size 14,1.6 mm (0.06 in) ID,
3.1 m (10 ft)
56-4106-17
PTSL03-10
Recirculation Pump Tubing, silicone, size 16, 3.1 mm (0.12 in) ID,
3.1 m (10 ft)
56-4105-75
RBFL-1
Female Luer to 1/8-in barb; nylon, pkg of 10
56-4105-76
RBFL-2
Female Luer to 3/32-in barb; nylon, pkg of 10
56-4105-83
KMRV-030
MidGee Reservoir Kit, 30 ml capacity, includes: (12) each
reservoirs and caps; (2) sets cap fittings; (12) sets reservoir
internal tubing
56-4105-84
KMRV-120
MidGee Reservoir Kit, 120 ml capacity, includes: (12) each
reservoirs and caps; (2) sets cap fittings; (12) sets reservoir
internal tubing
56-4105-86
KMDG-175R01A
Autoclavable MidGee Reservoir Kit, includes: (3) polycarbonate
reservoirs, 175 ml capacity, conical bottom; (3) solid reservoir
caps; (3) caps with Luer ports; (3) reservoir bases to fit MidJet
system; (1) standard reservoir base
56-4105-87
KMDG-175R02A
Autoclavable MidGee Reservoir Replacement
Kit.Includes: (6) polycarbonate reservoirs,
175 ml capacity, conical bottom; (3) solid reservoir caps; (6) caps
with three Luer ports; silicone tubing
Note: Kit KMDG-175R01A required for reservoir bases
56-4106-15
PT-109-02
Replacement Pressure Transducers (set of two)
Note: Requires calibration
56-4106-37
MDG-3SP
Basic MidJet System, includes: miniature peristaltic pump* with
plug-in power supply; exchangeable saddles for size 14 and 16
tubing; mounting platform**; (6) 30 ml reservoirs, caps, internal
tubing sets; (12) 120 ml reservoirs, caps, internal tubing sets; (4)
sets cap fittings; (1) backpressure valve; (10) Luer to 1/8-in tubing
adaptors; (10) Luer to 3/32-in tubing adaptors; silicone tubing,
sizes 14 and 16, 1.5 m each
56-4106-38
MDG-4SP
Advanced MidJet System, same as MDG-3SP plus: PRT-DPM-2
Pressure Transducers and Digital Panel Meter Readout
*Maximum nominal recirculation rate: 50 ml/min with size 14 tubing;
140 ml/min with size 16 tubing. Pump not sold separately.
**Mounting platform not sold separately.
Data File 18-1168-04 AC
7
www.gehealthcare.com
Global Headquarters GE Healthcare
Little Chalfont
Buckinghamshire, U.K. HP7 9NA
GE tagline and GE Monogram are trademarks of
General Electric Company.
All goods and services are sold subject to the terms and
conditions of sale of the company within GE Healthcare which
supplies them. GE Healthcare reserves the right, subject to any
regulatory and contractual approval, if required, to make changes
in specifications and features shown herein, or discontinue the
product described at any time without notice or obligation.
© 2005 General Electric Company–All rights reserved.
Amersham Biosciences AB, a General Electric Company going to
market as GE Healthcare.
GE Healthcare Amersham Biosciences AB
Björkgatan 30, 751 84 Uppsala, Sweden
GE Healthcare Amersham Biosciences Europe GmbH
Munzinger Strasse 9, D-79111 Freiburg, Germany
GE Healthcare Amersham Biosciences UK Ltd
Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
GE Healthcare Amersham Biosciences Corp
800 Centennial Avenue, P.O. Box 1327, Piscataway,
NJ 08855-1327, USA
GE Healthcare Amersham Biosciences KK
Sanken Bldg. 3-25-1, Hyakunincho, Shinjuku-ku,
Tokyo 169-0073, Japan
GE Healthcare Amersham Biosciences Ltd
13/F, Tower 1, Ever Gain Plaza, 88 Container Port Road
Kwai Chung, New Territories, HONG KONG
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18-1168-04 AC 02/2006