DNA replication in budding yeast : Link between chromatin

DNA replication in budding yeast : Link between
chromatin conformation and kinetics of
replication
Claire PANCIATICI
(Ligne SWING, Synchrotron SOLEIL, Gif sur Yvette, France)
Mardi 6 décembre 2016 – 14h30
Amphithéatre INSTN - CEA
Genetic information carried in the cell nucleus must be faithfully duplicated to be
transmitted to daughter cells during cell division. The preparation and execution of the
DNA replication program is restricted to specific phases of the cell cycle. DNA replication
occurs on a complex template of DNA associated with proteins called chromatin that is
both influencing and influenced by DNA replication. This work aims at investigating the
link between chromatin conformation and the kinetics of DNA replication. In order to do
so, we combine several techniques.
Using flow cytometry, we follow the evolution of a cell population in the cell cycle with
regards to their DNA content. Combining small-angle scattering of neutrons (SANS) and
X-rays (SAXS), we analyze entire replicating cell population. Our results suggest an
increasing organisation of the chromatin before division. Moreover, we performed DNA
combing to study the replication program in single cells that suggests that initiation
events are more frequent than previously measured.
The jury members are :
Dr Arach Goldar, CEA, Thesis Director
Dr Javier Perez, Synchrotron Soleil, Co-supervisor
Pr Sebastien Bloyer, Paris Sud, Jury Charman
Dr Benoit Le Tallec, ENS, Reporter
Dr Jean-Marc Victor, UPMC, Reporter
Dr Benjamin Audit, ENS Lyon, Examiner
Pr Nick Gilbert, University of Edimburgh, Examiner
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