MOLECULAR BIOLOGY OF THE CELL 2012 - 2013 - HKU

MOLECULAR BIOLOGY OF THE CELL
2012 - 2013
(From 14 January to 15 February, 2013)
Directors of the course
Roberto BRUZZONE
HKU - Pasteur Research Centre
Hong Kong
Philippe CHAVRIER
Institut Curie - Research Section
Paris
Head of Studies
Chiara ZURZOLO
Institut Pasteur
Paris
Location
Centre d'Enseignement de l'Institut Pasteur
Pavillon « Louis Martin »
28, rue du Docteur Roux
75724 Paris Cedex 15
Lectures: Room No 1
Practical Work: Room on the first floor
DESCRIPTION OF THE COURSE
The Molecular Biology of the Cell course is an intensive laboratory and lecture course of five
weeks divided into weekly modules, each focusing on a cutting-edge aspect of cell biology. It is
composed of lectures given by internationally renowned scientists, and of two practical sessions
organized together with teams from the Curie and the Pasteur Institutes. The main topics of the
course alternate between the cell biology of infection, cancer and intracellular trafficking
emphasizing new experimental approaches. The availability of the core Imaging Platform at
Institut Pasteur will introduce students to advanced techniques for the dynamic visualization of
cells in health and disease.
The maximum number of participants is 24, which includes a selected number of Master 2
students from the University of Paris 6, Paris 7 and Paris XI and foreign postgraduate students
in an interactive classroom setting. The course is intended to be a platform of excellence in
which students can meet and closely interact with worldwide top-level scientists to discuss,
exchange ideas and establish valuable contacts in the perspective of establishing a network of
young cell biologists at an early stage in their careers. Students will be able to understand the
importance of basic research and of a broad interdisciplinary approach to improve human
health. We also expect to provide orientations and mentoring to help students in their future
career.
The 2012-2013 course includes two practical sessions of one week each, focused respectively on
endocytosis and signaling and on tracking bacterial invasion and identification of virulence
factors. At the end of each session students are expected to present and discuss their
experiments. At the end of the course there is an exam consisting in the presentation of an
imaginary follow-up project based on the critical analysis of a recent scientific article. Students
submit a written document (4-5pages) and then discuss their project following a slide
presentation. The final evaluation is based on the participation in the course, the presentation of
the practical work and the final exam.
Practical work 1
MECHANISMS OF ENDOCYTOSIS
Endocytosis plays a fundamental role in cellular homeostasis and is required for the cellular
uptake of nutrients, controls plasma membrane turnover, the signaling response to hormones
and growth factors, antigen presentation or transport of molecules through epithelia. Moreover,
many infectious virus, bacteria, or protozoa take advantage of endocytosis to infect cells directly
or indirectly via toxins with intracellular activities.
Shiga toxin is getting endocytosed in a clathrin independent manner and is reaching the
endoplasmatic reticulum via the retrograde route.
First, the students will study retrograde trafficking of Shiga toxin and learn how localized
perturbation of cellular machinery can influence trafficking of Shiga toxin.
Second, the students will use a quantitative assay to study endocytosis of Shiga toxin and
transferrin, which are clathrin-independent and clathrin-dependent cargos, respectively.
Third, a lipid model membrane (giant unilamellar vesicles, GUVs) will be introduced and the
students will learn how to generate GUVs, study phase separation and STxB driven membrane
mechanics.
Practical work 2
CYTOSKELETAL REGULATION DURING ASTROCYTE POLARIZATION AND MIGRATION
Cell migration not only participates in morphogenetic movements during development, but also
contributes to tissue renewal, immune responses, wound healing and cancer cell dissemination
in adult organisms. Migration is a polarized cellular process, which opposes a protrusive front
edge to a retracting trailing edge. From the front to the rear, cytoskeletal rearrangements play a
key role in promoting the different processes leading to cell migration.
The students will use primary astrocytes in a scratch-induced migration assay to
- Identify the changes in morphology and in cytoskeletal organization in migrating astrocytes.
- Determine how alteration of cytoskeletal rearrangements modulate astrocyte migration
- Assess the role of RhoGTPases in the regulation of the cytoskeletal elements and adhesion
complexes during astrocyte migration and conclude on their functions during astrocyte
polarization and directed migration
*****
GENERAL INTRODUCTION
THE ORGANIZATION OF THE CELL
1st WEEK
Doctoral School module 1 “Organization of the cell”
Monday, 14 January
9:00 - 16:00
Introduction
Welcome and administrative matter
Institut Pasteur Registration Office
Presentation of the scope and overall
organization of the course
► Presentation of the practical sessions with
organizing teams
► Presentation of the exam and paper assignment
►
Roberto BRUZZONE
(HKU-Pasteur Research Centre)
Philippe CHAVRIER
(Institut Curie, France)
Chiara ZURZOLO
(Institut Pasteur, France)
Student presentations
16:00 -18:00
Chromatin assembly from nucleosome
to nuclear organization
Tuesday, 15 January
Geneviève ALMOUZNI
(Institut Curie, France)
Nucleus and Epigenetics
INSTITUT CURIE DAY
Location : Amphithéâtre Lacassagne,
Institut Curie
26 rue d’Ulm
75005 Paris
9:00 - 11:00
11:00 - 13:00
Chromosomes dynamics and epigenetic changes
during X inactivation
Nuclear organization during tissue differentiation:
dealing with heterochromatin
13:00 - 14:15
Lunch for students and speakers (Chez Marie)
14:30 - 16:30
The epigenome: images from reality
Edith HEARD
(Institut Curie, France)
Susan GASSER
(FMI, Switzerland)
Valerio ORLANDO
(Dulbecco Telethon Institute, Italy)
16:30 - 17:15
Epigenetics during bacterial infections
Mélanie HAMON
(Institut Pasteur, France)
Wednesday, 16 January
9:00 - 10:30
Cell division and cytoskeleton
How cells divide
Helder MAIATO
(IBMC, Lisbon, Portugal)
11:00 - 12:30
14:00 - 15:30
Cytoskeleton and membrane dynamics in the final
steps of cell division
Actin dynamics and cell migration
Arnaud ECHARD
(Institut Pasteur, France)
Alexis GAUTREAU
(CNRS, France)
16:00 - 17:30
Posttranslational modifications of tubulins: more
than simple switches
Thursday, 17 January
8:45 - 10:00
Carsten JANKE
(Institut Curie, France)
Frontiers in microscopy
Quantitative biologicals imaging
Jean-Christophe OLIVO-MARIN
(Institut Pasteur, France)
10:15 - 11:15
11:45 - 12:45
14:30 - 15:30
15:45 - 16:45
The targeting of host cell compartments
by intracellular bacterial pathogens
Jost ENNINGA
(Institut Pasteur, France)
Dissecting immune responses using intravital
two-photon imaging
Philippe BOUSSO
Intravital microscopy: a power tool to study
biological processes in live animals
Roberto WEIGERT
Dynamic imaging of cellular function
(Institut Pasteur, France)
(National Institute of Health, USA)
David DIGREGORIO
(Institut Pasteur, France)
17:15 - 18:15
Following single molecules in live and fixed cells
to understand transcription regulation
Friday, 18 January
Xavier DARZACQ
(Ecole Normale Supérieure, France)
Institut Pasteur - Institut Curie Day
Location: Auditorium Centre François Jacob (building 26)
13:30 - 14:30
14:30 – 15:30
Single-molecule biology and visualization of
endocytosis
Intravital microscopy as a tool to study membrane
traffic and cell biology in live animals
15:30 - 16:00
Coffee Break
16:00 - 16:35
Glycosphingolipid function in the initiation
of clathrin-independent endocytosis
16:35 - 17:10
Cortical remodelling and cell division
Tom KIRCHHAUSEN
(Harvard Medical School, USA)
Roberto WEIGERT
(National Institute of Health, USA)
Ludger JOHANNES
(Institut Curie, France)
Arnaud ECHARD
(Institut Pasteur, France)
17:10 - 18:10
18:15 - 19:00
Endosomal sorting: from mechanisms of membrane
deformation and cargo selection, through too
functional outcomes for organism development
Cocktail
*****
Peter CULLEN
(University of Bristol, UK)
MECHANISM
OF INTRACELLULAR TRAFFICKING,
ENDOCYTOSIS AND BACTERIAL INFECTION
2nd WEEK
Practical work: L. Johannes
Doctoral School module 2 “Membrane mechanics of endocytosis”
Monday, 21 January
9:00 - 11:00
11:00 -12:00
13:30 - 19:00
Molecular mechanism of clathrin coat
formation
Tom KIRCHHAUSEN
(Harvard Medical School, USA)
Endocytosis and retrograde trafficking:
mechanisms and applications
Ludger JOHANNES
Practical work 1:
Mechanisms of uptake into cells:
Shiga toxin endocytosis and trafficking via
the retrograde route
Ludger JOHANNES, Guylène K’OUAS
and Christian WUNDER
(Institut Curie, France)
Covalent conjugation of Shiga toxin with a fluorescent
probe and chromatographic purification
Tuesday, 22 January
9:00 - 11:00
11:00 - 19:00
Multiple functions of myosin motor proteins
in membrane trafficking pathways
Folma BUSS
(University Cambridge, UK)
Practical work 1 (continuation):
Study the retrograde transport of fluorescent Shiga toxin from the plasma membrane
to the endoplasmatic reticulum, including perturbation by ATP-depletion or the Retro-2
inhibitor. Immunofluorescence staining and microscopy.
Wednesday, 23 January
9:00 - 11:30
Some physical aspects of membrane trafficking
Patricia BASSEREAU
(Institut Curie, France)
11:30 - 19:00
Practical work 1 (continuation):
Quantitative analysis of endocytosis (Shiga toxin and transferrin) by ELISA
Thursday, 24 January
9:00 - 11:30
11:30 - 19:00
The fascinating strategies used by Listeria
during infection
Pascale COSSART
(Institut Pasteur, France)
Practical work 1 (continuation):
Continuation of ELISA and microscopy of immunofluorescence samples (day2)
Friday, 25 January
9:00 - 11:30
11:30 - 19:00
Transendothelial leukocyte migration: integration of
adhesive and chemotactic cues under shear forces
Ronen ALON
(Weizmann Inst. of Science, Israel)
Practical work 1(continuation):
Generation of giant unilamellar vesicles (GUVs) by electroformation.
Study of phase separation and STxB driven membrane mechanics on GUVs.
*****
CELL MIGRATION, CYTOSKELETON AND TUMOR INVASION
3rd WEEK
Practical work: S. Etienne-Manneville
Doctoral School module 3 “Cell migration, cytoskeleton and tumor invasion”
Monday, 28 January
8:30 - 9:30
Practical work 2: “Cytoskeletal regulation
during astrocyte polarization and migration”
Sandrine ETIENNE-MANNEVILLE
and Guylène K’OUAS
Day 1 morning: cell treatment and scratch assay
9:30 - 11:30
Rho GTPase signaling and cell migration
Anne RIDLEY
(King’s College London, UK)
12:00 - 13:00
14.00 - 18.00
seminar BCI
Roles of Rho GTPases in cancer cell invasion
Practical work 2 (continuation):
Day 1 afternoon:
- si RNA nucleofection
- End of the scratch assay. Cell fixation
- Beginning of videomicroscopy (Gr 2, 6, 10)
Anne RIDLEY
Sandrine ETIENNE-MANNEVILLE
and Guylène K’OUAS
Tuesday, 29 January
9:00 - 11:30
13:00 - 19:00
Astrocyte polarization and
migration
Sandrine ETIENNE-MANNEVILLE
(Institut Pasteur, France)
Practical work 2 (continuation) Day 2:
- Immunofluorescence
- Preparation of primary astrocytes
- Beginning of videomicroscopy (Gr 4, 8, 12)
Wednesday, 30 January
9:00 - 11:30
13:00 - 19:00
Neuronal migration in the developing
brain
Oscar MARIN PARRA
(Instituto de Neurociencias, UMH, Spain)
Practical work 2 (continuation) Day 3
- Microscopy
- Result analysis
- Beginning of videomicroscopy (Gr 1, 5, 9)
Thursday, 31 January
9:00 - 9:30
Practical work 2 (continuation) Day 4 morning
Scratch assay on nucleofected cells
9:30 - 12:00
Cell biology of tumor cell invasion
Philippe CHAVRIER
(Institut Curie, France)
13:30 - 19:00
Practical work 2 (continuation) Day 4 afternoon
- Cell lysis, electrophoresis and transfert
- End of the scratch assay. Cell fixation.
- Beginning of videomicroscopy (Gr 3, 7, 11)
Friday, 01 February
9:00 - 11:30
13:00 - 19:00
Using vaccinia virus a tool to dissect cytoskeletal
dynamics and signalling networks
Practical work 2 (continuation) Day 5
- Western blot
- Immunofluorescence
*****
Michael WAY
(Cancer Research, UK)
ANALYTICAL DISSECTION OF THE PRACTICAL WORKSHOPS
4th WEEK
Monday, 4 February
9:00 - 12:00
Practical work 2 (end): microscopy, result analysis
and preparation of general discussion
12:00 - 13:00
Seminar BCI
The diaries of a seasoned traveller:
axonal transport and sorting of signalling endosomes
14:00 - 18:00
Giampietro SCHIAVO
(Cancer Research, UK)
Practical work 2 (end): microscopy, result analysis
and preparation of general discussion
Tuesday, 5 February
9:00 - 10:30
Motor proteins
Giampietro SCHIAVO
(Cancer Research, UK)
11:00 - 13:00
14:00 - 16:00
General discussion of Practicals 2
(presentations of students)
Giampietro SCHIAVO
(Discussion animator)
General discussion of Practicals 2
(presentations of students)
Wednesday, 6 February
9:00 - 19:00
Practical work 1(end):
Microscopy of immunofluorescence samples (day 2).
11:00 - 13:00
Optogenetic dissection of neural circuits function
and development
Filippo DEL BENE
(Institut Curie, France)
Thursday, 7 February
9:00 - 10:30
11:00 - 12:30
A career around science: bringing
science out of the lab
Katja-Stina MAGNUSSON
(ISARIC, UK)
On scientific writing and the making of a presentation
Roberto BRUZZONE
(Pasteur Research Center, Hong Kong)
Friday, 8 February
9:00 - 11:00
11:30 - 13:00
14:00 - 16:00
Dynamics and mechanics of intracellular
signaling: role of caveolae
Christophe LAMAZE
General discussion of Practicals 1
(presentations of students)
Christophe LAMAZE
General discussion of Practicals 1
(Institut Curie, France)
(Discussion animator)
(Institut Curie, France)
PROJECT SUBMISSION AND EXAMS
5th WEEK
Monday 11 to Tuesday 12 February
Project preparation. The written project has to be submitted to the Course Commitee by Tuesday
night.
Wednesday 13 to Thursday 14 February
Preparation of the oral exam
Friday, 15 February
9:00 - 18:00
Final examination
18:00
Closing party
*****
Detailed description of the examination
Oral exam on Friday 15 February, 2013 (mark on a 1-20 scale, coefficient 1):
Critical analysis of a scientific article and presentation of an imaginary 3-year research project as
follow-up of the results of the article.
Presentation: 13 minutes, questions: 7 minutes, total duration: 20 minutes
Organization of the oral presentation:
- The presentation is open to the public
- Slides (Powerpoint or other supported formats)
The scientific articles will be given to the students at the end of the first week of the course. As
follow-up of the article received each student will write a fictional project in a 4/5 page
document that should be submitted to the members of the jury no later than Tuesday 12
February at 20:00. This document should include:
- Summary of the article (max 1 page)
- Aims and description of the project (max 3 pages including figures if appropriate)
- References (max 1 page)
Description détaillée de l’examen
Examen oral le vendredi 15 février 2013 (note sur 20, coefficient 1) :
Présentation critique d’un article et discussion d’un projet fictif sur 3 ans découlant de ces
résultats.
Présentation : 13 minutes, questions du jury : 7 minutes, durée totale : 20 minutes.
Organisation de la présentation orale :
- Exposé public de chaque étudiant devant le jury
- Diapositives (logiciel Powerpoint ou autre format compatible)
Les articles scientifiques seront donnés aux étudiants à la fin de la première semaine de cours. Le
projet fictif est présenté dans un document de 4/5 pages à remettre au jury au plus tard le mardi
12 février à 20h00, comprenant :
- Résumé de l’article (max 1 page)
- Objectifs et description du projet (max 3 pages, figures incluses)
- Bibliographie (max 1 page)
*****
Centre d’Enseignement
MOLECULAR BIOLOGY OF THE CELL COURSE
2012 - 2013
ADDRESS DETAILS
*****
Name
First name
email
Institution
Address
City
Country
Telephone
ORGANIZERS OF THE COURSE
Mr
BRUZZONE
Roberto
[email protected]
HKU-Pasteur
Research Centre
1/F Dexter HC
Man Building,
8 Sassoon Road
Pokfulam,
Hong Kong
Hong Kong
852-28168403
26, rue d'Ulm
75248 Paris
Cedex 05
France
33-(0)156246359
25/28 rue du Dr
Roux
75724 Paris
Cedex 15
France
33-(0)145688277
Mr
CHAVRIER
Philippe
[email protected]
Membrane & Actin Dynamics
Lab,
CNRS/UMR144,
Institut Curie
Ms
ZURZOLO
Chiara
[email protected]
Membrane Trafficking and
Pathogenesis Unit,
Institut Pasteur
LECTURERS
Ms
ALMOUZNI
Geneviève
[email protected]
UMR 218 CNRS
Institut Curie
26, rue d'Ulm
75248 Paris
Cedex 05
France
33-(0)156246701
Mr
ALON
Ronen
[email protected]
Dept. of Immunology,
Weizmann Institute of Science
Wolfson Bldg.,
Room 330
Rehovot 76100
Israel
972-8 934 2482
Ms
BASSEREAU
Patricia
[email protected]
Physico-chimie Curie,
Institut Curie Research Center
26, rue d'Ulm
75248 Paris
Cedex 05
France
Ms
BOUSSO
Philippe
[email protected]
75724 Paris
Cedex 15
France
33-(0)1 45 68 85 51
Ms
BUSS
Folma
[email protected]
Cambridge CB2
0XY
UK
44-1223 763348
Ms
COSSART
Pascale
[email protected]
Unité des Interactions
Bactéries-Cellules,
Institut Pasteur
75724 Paris
Cedex 15
France
33-(0)1 45 68 88 41
[email protected]
Henry Wellcome Integrated
Signaling Laboratories,
Department of Chemistry,
School of Medical Sciences,
University of Bristol
Bristol BS8 1TD
UK
44-117 3312193
Mr
CULLEN
Peter
Unité des Dynamiques des
25/28 rue du Dr
Réponses Immunes,
Roux
Institut Pasteur
University of Cambridge,
Cambridge Institute for
Hills Road
Medical Research,
Wellcome Trust/MRC Building,
25/28 rue du Dr
Roux
Name
Mr
DARZACQ
First name
Xavier
email
Institution
Address
City
Country
[email protected]
Functional Imaging of
Transcription,
Institut de Biologie de l'Ecole
Normale Supérieure,
CNRS UMR 8197
46, rue d'Ulm
75230 Paris
Cedex 05
France
26, rue d'Ulm
75248 Paris
Cedex 05
France
Telephone
Mr
DEL BENE
Filippo
[email protected]
Génétique & Biologie du
Développement,
CNRS UMR3215 / INSERM
U934,
Institut Curie
Mr
DIGREGORIO
David
[email protected]
Unit of Dynamic Neuronal
Imaging,
Institut Pasteur
25/28 rue du Dr
Roux
75724 Paris
Cedex 15
France
33-(0)1 45 68 80 54
Mr
ECHARD
Arnaud
[email protected]
Membrane Traffic & Cell
Division Lab
Institut Pasteur
25/28 rue du Dr
Roux
75724 Paris
Cedex 15
France
33-(0)1 44 38 94 09
Mr
ENNINGA
Jost
[email protected]
Groupe Dynamique
25/28 rue du Dr
Interactions Hôte-Pathogène,
Roux
Institut Pasteur
75724 Paris
Cedex 15
France
33-(0)1 44 38 94 13
Ms
ETIENNEMANNEVILLE
Sandrine
[email protected]
25/28 rue du Dr
Roux
75724 Paris
Cedex 15
France
33-(0)1 40 61 39 05
Ms
GASSER
Susan
[email protected]
Friedrich Miescher Institute for Maulbeerstrasse
Biomedical Research
66
4058 Basel
Switzerland
41-61 697 5025
Mr
GAUTREAU
Alexis
[email protected]
Laboratoire d'Enzymologie et
Biochimie Structurales,
UPR3082 CNRS
Bât. 34,
Avenue de la
Terrasse
91198 Gif-surYvette Cedex
France
33-(0)1 69 82 35 08
Ms
HAMON
Mélanie
[email protected]
Unité des Interactions
Bactéries-Cellules,
Institut Pasteur
25/28 rue du Dr
Roux
75724 Paris
Cedex 15
France
33-(0)145688995
Ms
HEARD
Edith
[email protected]
INSERM U934 / CNRM
UMR3215,
Institut Curie
26, rue d'Ulm
75248 Paris
Cedex 05
France
33-(0)156246829
Unité de Polarité Cellulaire,
Migration & Cancer,
Institut Pasteur
Name
Mr
Mr
JANKE
JOHANNES
First name
Carsten
Ludger
email
Institution
Address
City
Country
Telephone
[email protected]
Institut Curie,
Dept. Signalling, Neurobiology
& Cancer,
CNRS UMR3306 - INSERM
U1005
Bat 110,
Centre
Universitaire
91405 Orsay
Cedex
France
33-(0)1 69 86 31 27
[email protected]
Laboratoire Trafic,
Signalisation & Criblage
Intracellulaire,
UMR 144,
Institut Curie
26, rue d'Ulm
75248 Paris
Cedex 05
France
33-(0)1 56 24 63 51
Boston,
MA 02115
USA
1-617 713 8682
33-(0)1 56 24 69 66
Mr
KIRCHHAUSEN
Tom
[email protected]
Immune Disease Institute,
Harvard Medical School
W. Alpert
Building,
Room 133,
200 Longwood
Ave.
Mr
LAMAZE
Christophe
[email protected]
UMR 144 Curie / CNRS,
Institut Curie
26, rue d'Ulm
75248 Paris
Cedex 05
France
[email protected]
ISARIC Project Manager,
University of Oxford,
Nuffield Department of
Medicine
Churchill
Hospital,
Old Road
Oxford OX3 7LJ
UK
Rue do Campo
Alegre 823
4150-180 Porto
Portugal
351-22 6074 900
Ms
MAGNUSSON
Kajsa-Stina
Mr
MAIATO
Helder
[email protected]
Chromosome Instability &
Dynamics Lab.,
Instituto de Biologia Molecular
e Celular,
Universidade do Porto
Mr
MARIN PARRA
Oscar
[email protected]
Instituto de Neurociencias,
UMH-CSIC
Avda. Ramon y
cajal s/n
03550 Sant Joan
d'Alacant
Spain
34-965 91 9415
Mr
OLIVO-MARIN
Jean-Christophe
[email protected]
Unité d'Analyse d'Images
Quantitative,
Institut Pasteur
25/28 rue du Dr
Roux
75724 Paris
Cedex 15
France
33-(0)1 45 68 85 06
Mr
ORLANDO
Valerio
[email protected]
Dulbecco Telethon Institute,
IRCCS Santa Lucia
Via del Fosso di
Fiorano 64
00143 Roma
Italy
39-06 501 703260
Name
First name
email
Institution
Address
City
Country
Telephone
Guy's Campus
London SE1 1UL
UK
44-(0)20 7848 6209
Ms
RIDLEY
Anne
[email protected]
King's College London,
Randall Division of Cell and
Molecular Biophysics,
New Hunt's House
Mr
SCHIAVO
Giampietro
[email protected]
Cancer Research UK,
London Research Institute
44 Lincoln's Inn
Fields
London WC2A
3LY
UK
44-207 269 3300
Mr
WAY
Michael
[email protected]
Cell Motility Laboratory,
Cancer Research UK,
London Research Institute
44 Lincoln's Inn
Fields
London WC2A
3LY
UK
44-207 269 3733
[email protected]
Intracellular Membrane
Trafficking Unit,
Oral and Pharyngeal Cancer
Branch,
National Institute of Dental
and Craniofacial Research,
NIH
30 Convent Dr.
Room 303A
Bethesda,
MD 20892-4340
USA
Mr
WEIGERT
Roberto
PRACTICAL WORK
Ms
Mr
K'OUAS
WUNDER
Guylène
Christian
[email protected]
Centre d'Enseignement,
Institut Pasteur
25/28 rue du Dr
Roux
75724 Paris
Cedex 15
France
[email protected]
Laboratoire Trafic,
Signalisation & Criblage
Intracellulaire,
UMR 144,
Institut Curie
26, rue d'Ulm
75248 Paris
Cedex 05
France
25/28 rue du Dr
Roux
75724 Paris
Cedex 15
France
33-(0)1 40 61 30 02
SECRETARY
Ms
COURMARCEL
Fabienne
[email protected]
Centre d'Enseignement,
Institut Pasteur
33-(0)1 40 61 38 79