Cat. No. GP62-C - PROGEN Biotechnik GmbH

Cat. No. GP62-C - PROGEN Biotechnik GmbH

PROGEN Biotechnik GmbH  Maaßstraße 30  69123 Heidelberg  Germany
Tel.: +49 (0) 6221 8278-0  Fax: +49 (0) 6221 8278-24  Email: [email protected]  Web: www.progen.de
Pab to
p62 Protein, C-terminal specific
Category
Guinea pig polyclonal (GP62)
Form/Purification
Stabilized antiserum
Antigen
C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ
YSK HPP PL) of human p62 protein, coupled to KLH. This
peptide sequence is identical in human, monkey, bovine,
mouse, and rat.
Reactive Polypeptide (Specificity)
Human 62 kD (p62) protein, is present in intracytoplasmic
inclusions (e.g. hyaline bodies) of hepatocellular carcinoma.
p62 protein (also described as ubiqutin-binding protein;
sequestosome 1; SQSTM1) has been found in many tissues
and cells, including lymphoid cells, serving probably a
common cellular signal transduction mechanism (e.g.
ubiquitin-associated degradation and autophagy). The
antiserum stains also neurofibrillary tangles in the brain of
patients suffering from Alzheimer’s disease.
Antigen Recognized in
Species (tested so far)
Human, mouse, rat
Application
Suitable for
- immunohistochemistry on frozen and paraffin*
sections (*enhanced after microwave treatment)
- cytological material
- immunoblotting
Working Dilution
1: 100 – 1:200
1:1,000 – 1:2000
Incubation Time
1 h at RT; extended with paraffin sections
Stability / Storage
At 2-8°C
Volume
100 µl; contains 0.09% sodium azide
Cat. No.
GP62-C
for immunohistochemistry
for Western blotting (ECL)
GP62-C/DS-270515; Page 1 of 2
PROGEN Biotechnik GmbH  Maaßstraße 30  69123 Heidelberg  Germany
Tel.: +49 (0) 6221 8278-0  Fax: +49 (0) 6221 8278-24  Email: [email protected]  Web: www.progen.de
References.
Stumptner C, Heid H, Fuchsbichler A, Hauser H, Mischinger H-J, Zatloukal K and Denk H: Analysis of
intracytoplasmic hyaline bodies in a hepatocellular carcinoma. Demonstration of p62 as major constituent.
Am J Pathol 154, 1701-1710 (1999).
Zatloukal K, Stumptner C, Fuchsbichler A, Heid H, Schnoelzer M, Kenner L, Kleinert R, Prinz M, Aguzzi A
and Denk H: p62 is a common component of cytoplasmic inclusions in protein aggregation diseases. Am
J Pathol 160, 255-263 (2002)
Stumptner C, Fuchsbichler A, Heid H, Zatloukal K, Denk H: Mallory Body - a disease-associated type of
sequestosome. Hepatology 35, 1053-1062 (2002)
Kuusisto E, Salminen A and Alafuzoff I: Early accumulation of p62 in neurofibrillary tangles in Alzheimer’s
disease: possible role in tangle formation. Neuropathology & Applied Neurobiology 28, 228-237 (2002)
Arai T, Nonaka T, Hasegawa M, Akiyama H, Yoshida M, Hashizume Y, Tsuchiya K, Oda T, Ikeda K:
Neuronal and glial inclusions in frontotemporal dementia with or without motor neuron disease are
immunopositive for p62. Neuroscience Letters 342, 41-44 (2003)
Furukawa Y, Iseki E, Hino H, Kanai A, Odawara T, Kosaka K: Ubiqitin and ubiquitin-related proteins in
neurons and dendrites of brains of atypical Pick’s disease without Pick bodies. Neuropathology 24, 38-45
(2004)
Müller T, Langner C, Fuchsbichler A, Heinz-Erian P, Ellemunter H, Schlenck B, Bavdekar AR, Pradhan
AM, Pandit A, Müller-Höcker J, Melter M, Kobayashi K, Nagasaka H, Kikuta H, Müller W, Tanner MS,
Sternlieb I, Zatloukal K, Denk H: Immunohistochemical analysis of Mallory Bodies in Wilsonian and NonWilsonian hepatic copper toxicosis. Hepatology 39, 963-969 (2004)
Janig E, Stumptner C, Fuchsbichler A, Denk H, Zatloukal K: Interaction of stress proteins with misfolded
keratins. Eur J Cell Biol 84, 329-339 (2005)
Kuusisto E, Kauppinen T, Alafuzoff I: Use of p62/SQSTM1 antibodies for neuropathological diagnosis.
Neuropathology & Applied Neurobiology pp 1-12 (2007)
BjørkøyG, Lamark T, Pankiv S, Øvervatn A, Brech A, Johansen T: Monitoring autophagic degradation of
p62/SQSTM1. Methods Enzymol 452, 181-197 (2009)
Aishima S, Fujita N, Mano Y, Iguchi T, Taketomi A, Maehara Y, Oda Y, Tsuneyoshi M: p62+ hyaline
inclusions in intrahepatic cholangiocarcinoma associated with viral hepatitis or alcoholic liver disease. Am j
Clin Pathol 134,457-465 (2010)
Itakura E, Mizushima N: p62 targeting to the autophagosome formation site requires self-oligomerization
but not LC3 binding. J Cell biol 192, 17-27 (2011)
Zatloukal B, Kufferath I, hueringer A, Landegren U, Zatloukal K, Haybaeck J: Sensitivity and Specificity of
In situ Proximity Ligation for Protein Interaction Analysis in a Model of Steatohepatitis with Mallory-Denk
Bodies. PLOS One; 5;9(5):e96690 (2014)
Ciuffa R, Lamark T, Tarafder AK, Guesdon A, Rybina S, Hagen WJH, Johansen T, Sachse C : The
selective autophagy receptor p62 forms a flexible filamentous helical Scaffold. Cell Reports 11, 748-758
(2015) ; http://dx.doi.org./10.1016/j.celrep.2015.03.062
Cat. No.
GP62-C
GP62-C/DS-270515; Page 2 of 2